Propagation method of boluo red beans

Abstract

The invention provides a propagation method of boluo red beans. The propagation method of the ormosia esculenta comprises the following steps: acquiring ormosia esculenta branches, wherein the ormosia esculenta branches are lateral branches growing on ormosia esculenta main branches; carrying out girdling operation on the boleya azalea branches so as to form annular cuts in the circumferential direction of the boleya azalea branches, and stripping the annular cuts to xylem; carrying out shading treatment on the annular incision so as to form calluses at the annular incision; performing tree-leaving treatment on the boleya ormosia branches subjected to shading treatment; and transplanting the boleya ormosia branches subjected to tree separation treatment. By means of the propagation method of the ormosia esculenta, rapid and efficient propagation of the ormosia esculenta can be achieved.

Description

Technical Field

[0001] The invention relates to the technical field of plant propagation, in particular to a propagation method of Boluo red beans. Background Art

[0002] There are very few Boluo red beans. It is a very small species belonging to the genus Red Bean of the Fabaceae family. It has simple leaves, panicles or racemes, obovate to oblong or rhombus pods, heavy seeds, and red seed coat. Affected by the natural distribution and population size, the natural flowering and fruiting rate of Boluo red beans is low, the seed germination time is long, and the germination rate is low. The mature seeds are brightly colored and are easily eaten by birds, squirrels, pests, etc., resulting in a low natural reproduction rate. If Boluo red beans are allowed to reproduce naturally, it will be difficult to meet the needs of population rejuvenation and social development, and there is a risk of endangerment. Therefore, it is urgently necessary to use other technical means to achieve the goal of rapid and efficient breeding. Summary of the invention

[0003] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a method for breeding Boluo red beans which can realize rapid and efficient breeding of Boluo red beans.

[0004] The objective of the present invention is achieved through the following technical solutions:

[0005] A breeding method for Boluo red beans comprises the following steps:

[0006] Obtaining a Boluo Red Bean branch, wherein the Boluo Red Bean branch is a side branch growing on a Boluo Red Bean main branch;

[0007] Performing a girdling operation on the Boluo Red Bean branch, so that a circular incision is formed in the circumference of the Boluo Red Bean branch, and the circular incision is peeled off to the wood part;

[0008] The annular incision is subjected to light shielding treatment so that callus tissue is formed at the annular incision;

[0009] The shading-treated red bean branches are removed from the tree;

[0010] The Boluo red bean branches after the tree removal treatment are transplanted.

[0011] In one embodiment, the Boluo red bean branches are semi-lignified, strong branches of the current year selected from the south, with complete terminal buds and intact leaves.

[0012] In one embodiment, the diameter of the Boluo red bean branch is 0.5 cm to 0.7 cm, and the length is about 20 cm to 30 cm.

[0013] In one embodiment, the annular incision is formed at the base of the Boluo red bean branch.

[0014] In one embodiment, the width of the annular incision is 0.3 cm to 0.5 cm.

[0015] In one embodiment, the annular cutout is subjected to light shielding treatment, which specifically comprises the following steps:

[0016] Performing a wrapping and covering process on the annular incision so as to place the annular incision in a light-shielding environment;

[0017] The annular incision after the winding and covering treatment is subjected to a generation treatment so as to generate callus tissue at the annular incision.

[0018] In one embodiment, the annular incision after the wrapping and covering process is subjected to a generation process, and the processing time is 20 days to 30 days.

[0019] In one embodiment, the shading-treated red bean branches are removed from the tree, and the specific steps are as follows:

[0020] Pruning is performed at the base of the Boluo red bean branch and between the annular incision and the main branch to separate the Boluo red bean branch from the main branch.

[0021] In one embodiment, the transplanting operation of the Boluo red bean branches after the tree removal treatment specifically comprises the following steps:

[0022] The base of the Boluo red bean branch is moistened to make the annular incision moist environment;

[0023] Carrying out planting treatment on the Boluo red bean branches after the branch and leaf pruning treatment;

[0024] The Boluo red bean branches after the pruning of branches and leaves are subjected to a planting treatment so that the Boluo red bean branches are cut into a substrate.

[0025] In one embodiment, the matrix includes perlite and vermiculite.

[0026] Compared with the prior art, the present invention has at least the following advantages:

[0027] The Boluo red bean propagation method of the present invention forms annular incisions on the circumference of the Boluo red bean branches, the annular incisions are peeled off to the wood part, and the callus tissue of the Boluo red bean is directly formed in an in vitro state, and the formation of the annular incisions effectively reduces the influence of environmental factors on the generation of the callus tissue at the annular incisions of the Boluo red bean, and the annular incisions are conducive to the formation of annular callus tissue on the Boluo red bean branches, promote the formation of annular root growth areas, are conducive to the cutting rooting growth of the Boluo red bean branches, and are conducive to improving the full generation of the callus tissue at the annular incisions of the Boluo red bean, that is, not only the influence of environmental factors on the generation of the callus tissue is reduced, but also the growth of the callus tissue of the annular incisions of the Boluo red bean is facilitated, thereby improving the later differentiation strength of the callus tissue of the annular incisions of the Boluo red bean, thereby improving the rooting ability of the Boluo red bean branches, and improving the propagation survival rate of the Boluo red bean. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without creative work.

[0029] Figure 1 A flow chart of a method for propagating Boluo red beans according to an embodiment of the present invention;

[0030] Figure 2 It is a structural schematic diagram of a cutting fixture according to an embodiment of the present invention;

[0031] Figure 3 for Figure 2 Another structural schematic diagram of the cutting fixture shown;

[0032] Figure 4 for Figure 2 A cross-sectional view of the cutting fixture shown;

[0033] Figure 5 This is a recorded picture of Example 13 of the present invention. DETAILED DESCRIPTION

[0034] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the relevant drawings. The preferred embodiments of the present invention are given in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thoroughly understood.

[0035] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only and do not represent the only implementation method.

[0036] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0037] The present application provides a method for propagating Boluo red bean. The above-mentioned method for propagating Boluo red bean includes the following steps: obtaining Boluo red bean branches, which are side branches growing on the main branches of Boluo red bean; performing a ring cutting operation on the Boluo red bean branches so that a circular incision is formed in the circumference of the Boluo red bean branches, and the circular incision is peeled to the wood part; performing a light-shielding treatment on the circular incision so that callus tissue is formed at the circular incision; performing a tree-leaving treatment on the Boluo red bean branches after the light-shielding treatment; and performing a transplanting operation on the Boluo red bean branches after the tree-leaving treatment.

[0038] The above-mentioned propagation method of Boluo red bean forms an annular incision on the circumference of the Boluo red bean branch, and the annular incision is peeled to the wood part, and the callus tissue of Boluo red bean is directly formed in an in vitro state, which cooperates with the formation of the annular incision, effectively reducing the influence of environmental factors on the generation of callus tissue at the annular incision of Boluo red bean, and the annular incision is conducive to the formation of annular callus tissue on the Boluo red bean branch, promoting the formation of annular root growth area, which is conducive to the rooting growth of the Boluo red bean branch and is conducive to improving the full generation of callus tissue at the annular incision of Boluo red bean, that is, not only reducing the influence of environmental factors on the generation of callus tissue, but also conducive to the growth of callus tissue of the annular incision of Boluo red bean, thereby improving the later differentiation intensity of the callus tissue of the annular incision of Boluo red bean, thereby improving the rooting ability of the Boluo red bean branch and improving the propagation survival rate of Boluo red bean.

[0039] It should be noted that the principles of cuttings and tissue culture are both to produce new plants through asexual reproduction of plants, and both involve callus tissue. Specifically, cuttings are to insert the stems, leaves, buds and other parts of the plant into the growth medium, and use the growth hormones in the incision band to promote plant cell regeneration and proliferation to produce new plants. Its advantages are simple operation, high survival rate and easy mastery; its disadvantages are that the process of callus formation and further rooting of the stems, leaves, buds and other parts of the plant is easily affected by the environment, and the survival rate is not high; and tissue culture is to differentiate the nutritional tissue of the plant under sterile conditions, and then create an environment suitable for plant growth to cultivate seedlings. Its advantage is that it can quickly reproduce plants of the same variety in large quantities, and at the same time, it can improve the plants and cultivate new varieties; its disadvantages are that mutations are prone to occur, seedlings grow slowly, and the planting cost is high. Therefore, in the present application, in order to retain the shape of wild Boluo red beans, the cutting method is adopted to realize the reproduction of Boluo red beans, thereby realizing the rapid reproduction of Boluo red beans. In order to further reduce the impact of the environment on the cuttings of Boluo red beans and thus improve the survival rate of the cuttings, callus tissue is formed at the base of the undetached branches of Boluo red beans, which effectively reduces the impact of environmental factors on the formation of callus tissue at the annular incision of Boluo red beans. At this time, the reproduction survival rate of Boluo red beans is improved to a certain extent. Further, a circular incision is formed at the base of the Boluo red bean branches, and the circular incision is peeled to the xylem, which is conducive to improving the full generation of callus tissue at the annular incision of Boluo red beans, and improving the later differentiation intensity of the callus tissue of the annular incision of Boluo red beans, thereby improving the rooting ability of the Boluo red bean branches, and further improving the reproduction survival rate of Boluo red beans, thereby realizing the efficient reproduction of Boluo red beans.

[0040] In order to better understand the propagation method of Boluo red beans of the present application, the propagation method of Boluo red beans of the present application is further explained below:

[0041] See also Figure 1 A method for propagating red beans in Boluo in one embodiment comprises the following steps:

[0042] S100, obtaining Boluo red bean branches. The Boluo red bean branches are side branches growing on the main branches of Boluo red bean. A large number of branches are obtained, and the growth of the original Boluo red bean is less affected after the materials are obtained. In addition, the materials are obtained from naturally grown Boluo red bean, which better preserves the characteristics of Boluo red bean.

[0043] S200, girdling operation is performed on the Boluo red bean branches, so that the Boluo red bean branches are formed with annular incisions in the circumferential direction, and the annular incisions are peeled off to the wood part. It can be understood that the formation of annular incisions is conducive to the formation of annular callus on the Boluo red bean branches, promotes the growth area of ​​the root formed in annular shape, and is conducive to the cutting rooting growth of the Boluo red bean branches. If the incision is only a part of the Boluo red bean branches, i.e., an annular incision not around the periphery of the branches, the callus of the Boluo red bean branches will be further differentiated into the vascular cambium more difficult to control after generation, and then affect the cutting rooting of the Boluo red bean branches, that is, the callus formed by the Boluo red bean branches will be affected. Differentiation in the direction of the root.

[0044] S300, performing light shielding treatment on the annular incision so that callus tissue is formed at the annular incision, which is conducive to the rapid generation of callus tissue.

[0045] S400, the shading treated Boluo red bean branches are separated from the tree. It can be understood that the callus of Boluo red bean is directly formed in the in vitro state, and the formation of the annular incision is coordinated, which effectively reduces the influence of environmental factors on the generation of the callus at the annular incision of Boluo red bean, and is conducive to improving the full generation of the callus at the annular incision of Boluo red bean, that is, not only the influence of environmental factors on the generation of the callus is reduced, but also the growth of the callus of the annular incision of Boluo red bean is also conducive, thereby improving the differentiation intensity of the callus of the annular incision of Boluo red bean in the later stage, thereby improving the rooting ability of the Boluo red bean branches, and improving the reproduction survival rate of Boluo red bean.

[0046] S500, transplanting the branches of Boluo red bean after the tree removal treatment, thereby achieving better reproduction and growth of Boluo red bean.

[0047] The above-mentioned propagation method of Boluo red bean forms an annular incision on the circumference of the Boluo red bean branch, and the annular incision is peeled to the wood part, and the callus tissue of Boluo red bean is directly formed in an in vitro state, which cooperates with the formation of the annular incision, effectively reducing the influence of environmental factors on the generation of callus tissue at the annular incision of Boluo red bean, and the annular incision is conducive to the formation of annular callus tissue on the Boluo red bean branch, promoting the formation of annular root growth area, which is conducive to the rooting growth of the Boluo red bean branch and is conducive to improving the full generation of callus tissue at the annular incision of Boluo red bean, that is, not only reducing the influence of environmental factors on the generation of callus tissue, but also conducive to the growth of callus tissue of the annular incision of Boluo red bean, thereby improving the later differentiation intensity of the callus tissue of the annular incision of Boluo red bean, thereby improving the rooting ability of the Boluo red bean branch and improving the propagation survival rate of Boluo red bean.

[0048] In one embodiment, the branches of Boluo red bean are selected from the south and are semi-lignified, have complete terminal buds, and are strong branches of the current year with intact leaves, which better ensures the ability of the branches of Boluo red bean to form callus tissue, and further better ensures that the influence of environmental factors on the formation of callus tissue at the ring incision of Boluo red bean is reduced.

[0049] In one embodiment, the diameter of the Boluo red bean branch is 0.5 cm to 0.7 cm, and the length is about 20 cm to 30 cm, which better ensures the cutting propagation ability of the Boluo red bean branch.

[0050] In one embodiment, the annular incision is formed at the base of the Boluo red bean branch. Further, the inner wall of the annular incision is smooth and flat, which better ensures that the callus tissue is fully formed and the formed callus tissue has a higher differentiation strength.

[0051] In one embodiment, the distance between the annular incision and the main branch is 1 cm to 5 cm. Further, the distance between the annular incision and the main branch is 2 cm, which better reduces the impact on the original Boluo red bean and better ensures that the formed callus has a higher differentiation intensity.

[0052] In one embodiment, the width of the circular incision is 0.3 cm to 0.5 cm, which better ensures the amount of callus tissue formed, and further better ensures that the formed callus tissue has a higher differentiation intensity.

[0053] In one embodiment, the annular cutout is subjected to light shielding treatment, which specifically includes the following steps:

[0054] The annular incision is wrapped and covered to put it in a light-shielding environment.

[0055] Furthermore, the annular incision after the winding and covering treatment is subjected to a generation treatment so that callus tissue is generated at the annular incision.

[0056] In one of the embodiments, black tape is used to wrap the annular incision to shield light, which is beneficial to improving the adequacy and efficiency of callus tissue generation.

[0057] In one embodiment, the width of the black tape is at least the width of the annular cut. Further, the black tape is used to wrap the annular cut for at least two weeks to block light, which better ensures the air permeability in the annular cut and better ensures the shading effect at the annular cut.

[0058] In one embodiment, the annular incision after the winding and covering treatment is subjected to a forming treatment, and the treatment time is 20 days to 30 days, which better ensures the full formation of callus tissue.

[0059] In one of the embodiments, the Boluo red bean branches after shading treatment are removed from the tree, and the specific steps are: pruning is performed at the base of the Boluo red bean branches and between the annular incision and the main branch to separate the Boluo red bean branches from the main branch to avoid damaging the callus tissue, and it is helpful to reduce the damage to the callus tissue during the cutting process of the Boluo red bean branches, thereby better ensuring the differentiation ability of the formed callus tissue.

[0060] In one of the embodiments, the branches are pruned at a distance of 1 cm from the main branch through the circular incision. It can be understood that the branches are pruned at a distance of 1 cm from the main branch through the circular incision, which better avoids the destruction of the callus tissue, and makes the portion from the circular incision to the base of the Boluo red bean branch serve as the force-bearing section for cutting, and makes it shield the callus tissue at the circular incision during the cutting process of the Boluo red bean branch, and better reduces the damage of the callus tissue formed at the circular incision of the Boluo red bean by the matrix during the cutting process, and thus better ensures the differentiation ability of the formed callus tissue, thereby improving the rooting ability of the Boluo red bean branch and improving the reproduction survival rate of the Boluo red bean.

[0061] In one embodiment, the transplanting operation of the Boluo red bean branches after the tree removal treatment specifically includes the following steps:

[0062] The base of the Boluo red bean branch is moistened to create a moist environment for the annular incision, thereby better ensuring the moisture of the callus tissue at the annular incision of the Boluo red bean branch, and further better ensuring the differentiation ability of the callus tissue at the annular incision of the Boluo red bean branch.

[0063] Furthermore, the moistened red bean branches are pruned to reduce the water loss of the red bean branches after cutting.

[0064] Furthermore, the Boluo red bean branches that have been pruned are subjected to a planting process so that the Boluo red bean branches are cut into the substrate.

[0065] In one of the embodiments, the base of the Boluo red bean branch is moistened, and the specific steps are: wrap the base of the Boluo red bean branch with a wet towel, and wrap the annular incision with the wet towel as well, so as to better ensure the effective moistening of the callus tissue at the annular incision of the Boluo red bean branch.

[0066] In one of the embodiments, the moistened Boluo red bean branches are subjected to branch and leaf pruning, and the specific steps are: pruning the leaf buds on the Boluo red bean branches to retain at most 3 to 5 leaf buds, and cutting the Boluo red bean branches to make the length of the Boluo red bean branches 10 cm to 13 cm, and making the end of the Boluo red bean branch close to the base be cut obliquely, and the end of the Boluo red bean branch away from the base be cut flat, which is beneficial to improving the rooting growth of the Boluo red bean branches after cutting.

[0067] In one embodiment, a cutting jig is used to plant the Boluo red bean branches after the branches and leaves have been pruned. It can be understood that even if the portion from the annular incision to the base of the Boluo red bean branch is used as the force-bearing section for cutting, and it has a shielding effect on the callus tissue at the annular incision during the cutting of the Boluo red bean branch to reduce the damage to the callus tissue during cutting, the actual matrix will still interfere with the callus tissue at the annular incision of the Boluo red bean branch and cause mechanical damage to the callus tissue. If the method of adding the matrix later for covering is adopted, it will take more time to carry out the cutting of the Boluo red bean branch, especially for large-scale rapid propagation. For the cuttings of Boluo red bean branches cultivated by cultivation, it requires more manpower and material resources, the cost is high, and the efficiency is low; or the method of drilling holes in the substrate first is adopted, which still requires more manpower and material resources, and it is still difficult to effectively reduce the interference between the substrate and the callus tissue at the annular incision of the Boluo red bean branches and cause mechanical damage to the callus tissue. Therefore, in the present application, a cutting jig is used to carry out cuttings of Boluo red bean branches to complete the planting process, so as to reduce the cost of cuttings of Boluo red bean branches and improve the efficiency of cuttings of Boluo red bean branches.

[0068] For further information, please also refer to Figures 2 to 4 In one embodiment, the cutting fixture 10 includes a connecting piece 100 and two surrounding cutting inserts 200. The two surrounding cutting inserts 200 are arranged in a mirror image. The ends of the two surrounding cutting inserts 200 can be opened and closed by the connecting piece 100, so that the end of any surrounding cutting insert 200 away from the connecting piece 100 can be rotated in a direction away from the other surrounding cutting insert 200. Further, when the two wrapped plug-ins 200 are closed, the two wrapped plug-ins 200 enclose an anti-interference cavity 201, and the anti-interference cavity 201 is used to set the Boluo red bean branches so that the Boluo red bean branches and the substrate are avoided. A cutting wedge surface 202 is formed at one end of the anti-interference cavity 201, and a portion of the cutting wedge surface 202 is located on one of the wrapped plug-ins 200, and another portion of the cutting wedge surface 202 is located on the other wrapped plug-in 200. The cutting wedge surface 202 is located on either of the wrapped plug-ins 2 00 is on one end face away from the connecting piece 100, and an opening and closing clamping hole 203 is formed at the other end of the anti-interference cavity 201. Part of the opening and closing clamping hole 203 is located on one of the wrapped insertion plugs 200, and the other part of the opening and closing clamping hole 203 is located on the other wrapped insertion plug 200. The hole wall of the opening and closing clamping hole 203 is used to clamp the end of the Boluo red bean branch away from the base; when the two wrapped money plugs are opened, the anti-interference cavity 201 is exposed to the matrix, and the opening and closing clamping hole 203 opens and releases the Boluo red bean branch.

[0069] In this way, the Boluo red bean branch is placed on one of the wrapped grafting inserts 200, and then the other wrapped grafting insert 200 is merged to place the Boluo red bean branch in the anti-interference cavity 201, and the end of the Boluo red bean branch away from the base is clamped in the opening and closing clamping hole 203, which is simple to operate, and then the matrix is ​​opened by the cutting wedge surface 202 to realize the Boluo red bean branch in the matrix, and the two wrapped grafting inserts 200 are further opened to loosen the Boluo red bean branch. At this time, the entire cutting fixture 10 is pulled out to realize the cutting of the Boluo red bean branch, and in the process of cutting the Boluo red bean branch, the Boluo red bean branch and the matrix will not produce any mechanical interference, so that the integrity of the callus tissue at the annular incision of the Boluo red bean branch is better ensured, and then the differentiation ability of the formed callus tissue is better ensured, thereby improving the rooting ability of the Boluo red bean branch and improving the reproduction survival rate of Boluo red bean.

[0070] Please also read Figures 2 to 4 In one embodiment, the connecting member 100 is a torsion spring, one of which is wrapped around the insertion plug 200 and connected to one force arm of the torsion spring, and the other is wrapped around the insertion plug 200 and connected to the other force arm of the torsion spring, so that the two wrapped insertion plugs 200 are elastically opened and closed, thereby improving the convenience of using the insertion fixture 10.

[0071] Please also read Figures 2 to 4 In one embodiment, each wrapped insertion tool 200 is connected to one end close to the connector 100 with a hand 210, and the hand 210 of each wrapped insertion tool 200 at least partially protrudes from the end surface of the wrapped insertion tool 200, further improving the ease of use of the insertion fixture 10.

[0072] In one of the embodiments, two buffer members are provided on the hole wall of the opening and closing clamping hole, one of the buffer members is connected to one of the wrapped insertion members, and the other buffer member is connected to the other wrapped insertion member, and the end of the Boluo red bean branch away from the base is clamped between the two buffer members, thereby reducing the mechanical damage of the cutting jig to the Boluo red bean branch, thereby better reducing the impact on the growth of the Boluo red bean branch.

[0073] In one of the embodiments, the depth of the Boluo red bean branches cut into the substrate is 5 cm to 6 cm, which better ensures the rooting effect of the Boluo red bean branches.

[0074] In one embodiment, the matrix includes perlite and vermiculite. Further, the volume ratio of perlite to vermiculite is (3-5):(0.5-2). Further, the volume ratio of perlite to vermiculite is 4:1, which better ensures the rooting effect of the Boluo red bean branches.

[0075] In one embodiment, before the step of planting the Boluo red bean branches after the pruning treatment, and after the step of pruning the Boluo red bean branches after the moistening treatment, the Boluo red bean propagation method further includes the following steps: immersing one end of the Boluo red bean branches near the base in indolebutyric acid. Further, the concentration of indolebutyric acid is 800ml / L to 1200ml / L, which better ensures the rooting effect of the Boluo red bean branches.

[0076] In one embodiment, one end of the Boluo red bean branch close to the base is immersed in indolebutyric acid for 30 minutes to 50 minutes, which better ensures the rooting effect of the Boluo red bean branch.

[0077] After the step of transplanting the Boluo red bean branches that have been removed from the tree, the Boluo red bean propagation method further includes the following step: performing seedling bed management on the Boluo red bean branches.

[0078] In one embodiment, the seedbed management of the Boluo red bean branches is carried out, and the specific steps are: after the Boluo red bean branches are cut into the substrate, a shade net with a shading rate of 70% to 80% is covered at 1m to 3m above the seedbed. Further, after the Boluo red bean branches are cut into the substrate, a shade net with a shading rate of 75% is covered at 1.5m above the seedbed, which better ensures the rooting effect of the Boluo red bean branches.

[0079] In one embodiment, the humidity of the seedbed is 65% to 80%, and the temperature is 20° C. to 26° C. Further, the humidity of the seedbed is 75%, which further ensures the rooting effect of the Boluo red bean branches.

[0080] In one of the embodiments, after the step of managing the seedling bed of the Boluo red bean branches, the Boluo red bean propagation method further includes the following step: transplanting the Boluo red bean branches to better ensure the growth effect of the Boluo red bean branches.

[0081] In one embodiment, the Boluo red bean branches are transplanted, and the specific steps are: the Boluo red bean branches are cultivated in a seedbed for 35 days to 45 days, and then the Boluo red bean branches are transplanted into a mixed matrix, and the mixed matrix is ​​watered thoroughly. Further, the Boluo red bean branches are cultivated in the seedbed for 40 days, which better ensures the growth effect of the Boluo red bean branches.

[0082] In one embodiment, the mixed matrix includes perlite, peat soil and sand. Further, the volume ratio of perlite, peat soil and sand is (1.5-3): (1.5-3): (0.5-2). Further, the volume ratio of perlite, peat soil and sand is 2:2:1, which better ensures the growth effect of Boluo red bean branches.

[0083] In one embodiment, the mixed matrix is ​​sterilized with potassium permanganate solution and used for transplanting the branches of Boluo red bean. Further, the concentration of the potassium permanganate solution is 0.1%, which better ensures the growth effect of the branches of Boluo red bean.

[0084] In one of the embodiments, the Boluo red bean branches are transplanted into a mixed matrix, and after the mixed matrix is ​​watered thoroughly, the fermented soy milk is irrigated into the mixed matrix by root irrigation. Furthermore, the fermented soy milk is obtained by mixing beans and water in a volume ratio of 1:200 to form soy milk, and then sealed and fermented for 3 weeks, during which exhaust and resealing are performed every 3 days, and after the fermentation is completed, the volume ratio is 2:7 to form fermented soy milk. Furthermore, the fermented soy milk is irrigated into the mixed matrix by root irrigation, and the specific operation is: 3 holes are drilled in the mixed matrix, and the 3 holes are arranged around and adjacent to the Boluo red bean branches, and the fermented soy milk is poured into the holes, and the amount used is 5% to 10% of the volume of the matrix, which improves the survival rate of the Boluo red bean branches.

[0085] Compared with the prior art, the present invention has at least the following advantages:

[0086] The Boluo red bean propagation method of the present invention forms annular incisions on the circumference of the Boluo red bean branches, the annular incisions are peeled off to the wood part, and the callus tissue of the Boluo red bean is directly formed in an in vitro state, and the formation of the annular incisions effectively reduces the influence of environmental factors on the generation of the callus tissue at the annular incisions of the Boluo red bean, and the annular incisions are conducive to the formation of annular callus tissue on the Boluo red bean branches, promote the formation of annular root growth areas, are conducive to the cutting rooting growth of the Boluo red bean branches, and are conducive to improving the full generation of the callus tissue at the annular incisions of the Boluo red bean, that is, not only the influence of environmental factors on the generation of the callus tissue is reduced, but also the growth of the callus tissue of the annular incisions of the Boluo red bean is facilitated, thereby improving the later differentiation strength of the callus tissue of the annular incisions of the Boluo red bean, thereby improving the rooting ability of the Boluo red bean branches, and improving the propagation survival rate of the Boluo red bean.

[0087] Some specific examples are listed below, and if % is mentioned, it means percentage by weight. It should be noted that the following examples do not exhaust all possible situations, and the materials used in the following examples can be obtained from commercial sources unless otherwise specified.

[0088] Example 1

[0089] Preparation of cuttings: Select healthy and pest-free adult plants, select semi-lignified, complete terminal buds, intact leaves, 0.5 cm in diameter, and about 20 cm in length from the south of the plant, and make a ring cut at the base of the branch 1 cm away from the main branch. The ring cutting width is 0.3 cm. After ring cutting, use a blade to gently peel off the bark and scrape off the cambium to ensure that the incision is smooth and flat. Then wrap it with 3 cm wide black tape for 2 circles for light protection. After callus tissue is formed at the ring cutting site, cut the branch 1 cm below the ring cutting position, remove the black tape, wrap it with a wet towel to keep it moist and bring it back to the nursery for use;

[0090] Cutting medium: Use a mixture of perlite and vermiculite (3:0.5 volume ratio) as the cutting medium;

[0091] Cutting treatment: Cut off all leaves of the cuttings collected from the wild, and keep 3 buds at most. Cut the upper incision flat and the lower incision oblique. Soak the lower end of the cutting in 800ml / L indolebutyric acid solution for 50 minutes, take it out and directly insert it into the cutting medium, with an insertion depth of 5cm;

[0092] Seedbed management: After cutting, a shade net with a shading rate of 70% should be placed 1m above the seedbed. The humidity of the seedbed should be controlled at 65% and the temperature at 20℃.

[0093] Transplantation: 40 days after cutting, transplant the cuttings with intact roots into a pre-sterilized mixed substrate of perlite, peat soil, and sand (1.5:1.5:0.5). The substrate should be sterilized with 0.1% potassium permanganate solution before use and watered immediately after transplantation.

[0094] Example 2

[0095] Preparation of cuttings: Select healthy and pest-free adult plants, select semi-lignified, complete terminal buds, intact leaves, 0.6 cm in diameter, and about 25 cm in length from the south of the plant. Ring cut the branches 2 cm away from the main branches at the base, with a width of 0.4 cm. After ring cutting, use a blade to gently peel off the bark and scrape off the cambium to ensure that the cut is smooth and flat, and then wrap it with 3 cm wide black tape for 2 circles for light protection. After callus tissue is formed at the ring cutting site, cut the branches 1 cm below the ring cutting site, remove the black tape, wrap the moisturizing tape with a wet towel and bring it back to the nursery for use;

[0096] Cutting medium: Use a mixture of perlite and vermiculite (3:0.5 volume ratio) as the cutting medium;

[0097] Cutting treatment: Cut off all leaves of the cuttings collected from the wild, and keep 3 buds at most. Cut the upper incision flat and the lower incision oblique. Soak the lower end of the cutting in 800ml / L indolebutyric acid solution for 50 minutes, take it out and directly insert it into the cutting medium, with an insertion depth of 5cm;

[0098] Seedbed management: After cutting, a shade net with a shading rate of 70% should be placed 1m above the seedbed. The humidity of the seedbed should be controlled at 65% and the temperature at 20℃.

[0099] Transplantation: 40 days after cutting, transplant the cuttings with intact roots into a pre-sterilized mixed substrate of perlite, peat soil, and sand (1.5:1.5:0.5). The substrate should be sterilized with 0.1% potassium permanganate solution before use and watered immediately after transplantation.

[0100] Example 3

[0101] Preparation of cuttings: Select healthy and pest-free adult plants, select semi-lignified, complete terminal buds, intact leaves, 0.7 cm in diameter, and about 30 cm in length from the south of the plant. Ring cut the branches 5 cm away from the main branches at the base, with a width of 0.5 cm. After ring cutting, use a blade to gently peel off the bark and scrape off the cambium to ensure that the incision is smooth and flat, and then wrap it with 3 cm wide black tape for 2 circles for light protection. After callus tissue is formed at the ring cutting site, cut the branches 1 cm below the ring cutting site, remove the black tape, wrap the moisturizing tape with a wet towel and bring it back to the nursery for use;

[0102] Cutting medium: Use a mixture of perlite and vermiculite (3:0.5 volume ratio) as the cutting medium;

[0103] Cutting treatment: Cut off all leaves of the cuttings collected from the wild, and keep 3 buds at most. Cut the upper incision flat and the lower incision oblique. Soak the lower end of the cutting in 800ml / L indolebutyric acid solution for 50 minutes, take it out and directly insert it into the cutting medium, with an insertion depth of 5cm;

[0104] Seedbed management: After cutting, a shade net with a shading rate of 70% should be placed 1m above the seedbed. The humidity of the seedbed should be controlled at 65% and the temperature at 20℃.

[0105] Transplantation: 40 days after cutting, transplant the cuttings with intact roots into a pre-sterilized mixed substrate of perlite, peat soil, and sand (1.5:1.5:0.5). The substrate should be sterilized with 0.1% potassium permanganate solution before use and watered immediately after transplantation.

[0106] Example 4

[0107] Preparation of cuttings: Select healthy and pest-free adult plants, select semi-lignified, complete terminal buds, intact leaves, 0.6 cm in diameter, and about 25 cm in length from the south of the plant. Ring cut the branches 2 cm away from the main branches at the base, with a width of 0.4 cm. After ring cutting, use a blade to gently peel off the bark and scrape off the cambium to ensure that the cut is smooth and flat, and then wrap it with 3 cm wide black tape for 2 circles for light protection. After callus tissue is formed at the ring cutting site, cut the branches 1 cm below the ring cutting site, remove the black tape, wrap the moisturizing tape with a wet towel and bring it back to the nursery for use;

[0108] Cutting medium: Use a mixture of perlite and vermiculite in a ratio of 4:1 as the cutting medium;

[0109] Cutting treatment: Cut off all leaves of the cuttings collected from the wild, and keep 3 buds at most. Cut the upper incision flat and the lower incision oblique. Soak the lower end of the cutting in 800ml / L indolebutyric acid solution for 50 minutes, take it out and directly insert it into the cutting medium, with an insertion depth of 5cm;

[0110] Seedbed management: After cutting, a shade net with a shading rate of 70% should be placed 1m above the seedbed. The humidity of the seedbed should be controlled at 65% and the temperature at 20℃.

[0111] Transplantation: 40 days after cutting, transplant the cuttings with intact roots into a pre-sterilized mixed substrate of perlite, peat soil, and sand (1.5:1.5:0.5). The substrate should be sterilized with 0.1% potassium permanganate solution before use and watered immediately after transplantation.

[0112] Example 5

[0113] Preparation of cuttings: Select healthy and pest-free adult plants, select semi-lignified, complete terminal buds, intact leaves, 0.6 cm in diameter, and about 25 cm in length from the south of the plant. Ring cut the branches 2 cm away from the main branches at the base, with a width of 0.4 cm. After ring cutting, use a blade to gently peel off the bark and scrape off the cambium to ensure that the cut is smooth and flat, and then wrap it with 3 cm wide black tape for 2 circles for light protection. After callus tissue is formed at the ring cutting site, cut the branches 1 cm below the ring cutting site, remove the black tape, wrap the moisturizing tape with a wet towel and bring it back to the nursery for use;

[0114] Cutting medium: Use a mixture of perlite and vermiculite in a ratio of 4:0.5 as the cutting medium;

[0115] Cutting treatment: Cut off all leaves of the cuttings collected from the wild, and keep 3 buds at most. Cut the upper incision flat and the lower incision oblique. Soak the lower end of the cutting in 800ml / L indolebutyric acid solution for 50 minutes, take it out and directly insert it into the cutting medium, with an insertion depth of 5cm;

[0116] Seedbed management: After cutting, a shade net with a shading rate of 70% should be placed 1m above the seedbed. The humidity of the seedbed should be controlled at 65% and the temperature at 20℃.

[0117] Transplantation: 40 days after cutting, transplant the cuttings with intact roots into a pre-sterilized mixed substrate of perlite, peat soil, and sand (1.5:1.5:0.5). The substrate should be sterilized with 0.1% potassium permanganate solution before use and watered immediately after transplantation.

[0118] Example 6

[0119] Preparation of cuttings: Select healthy and pest-free adult plants, select semi-lignified, complete terminal buds, intact leaves, 0.6 cm in diameter, and about 25 cm in length from the south of the plant. Ring cut the branches 2 cm away from the main branches at the base, with a width of 0.4 cm. After ring cutting, use a blade to gently peel off the bark and scrape off the cambium to ensure that the cut is smooth and flat, and then wrap it with 3 cm wide black tape for 2 circles for light protection. After callus tissue is formed at the ring cutting site, cut the branches 1 cm below the ring cutting site, remove the black tape, wrap the moisturizing tape with a wet towel and bring it back to the nursery for use;

[0120] Cutting medium: Use a mixture of perlite and vermiculite in a ratio of 5:2 as the cutting medium;

[0121] Cutting treatment: Cut off all leaves of the cuttings collected from the wild, and keep 3 buds at most. Cut the upper incision flat and the lower incision oblique. Soak the lower end of the cutting in 800ml / L indolebutyric acid solution for 50 minutes, take it out and directly insert it into the cutting medium, with an insertion depth of 5cm;

[0122] Seedbed management: After cutting, a shade net with a shading rate of 70% should be placed 1m above the seedbed. The humidity of the seedbed should be controlled at 65% and the temperature at 20℃.

[0123] Transplantation: 40 days after cutting, transplant the cuttings with intact roots into a pre-sterilized mixed substrate of perlite, peat soil, and sand (1.5:1.5:0.5). The substrate should be sterilized with 0.1% potassium permanganate solution before use and watered immediately after transplantation.

[0124] Example 7

[0125] Preparation of cuttings: Select healthy and pest-free adult plants, select semi-lignified, complete terminal buds, intact leaves, 0.6 cm in diameter, and about 25 cm in length from the south of the plant. Ring cut the branches 2 cm away from the main branches at the base, with a width of 0.4 cm. After ring cutting, use a blade to gently peel off the bark and scrape off the cambium to ensure that the cut is smooth and flat, and then wrap it with 3 cm wide black tape for 2 circles for light protection. After callus tissue is formed at the ring cutting site, cut the branches 1 cm below the ring cutting site, remove the black tape, wrap the moisturizing tape with a wet towel and bring it back to the nursery for use;

[0126] Cutting medium: Use a mixture of perlite and vermiculite in a ratio of 4:1 as the cutting medium;

[0127] Cutting treatment: Cut off all leaves of the cuttings collected from the wild, and keep a maximum of 4 buds on each cutting. Cut the upper incision flat and the lower incision oblique. Soak the lower end of the cutting in a 1000ml / L indolebutyric acid solution for 40 minutes, take it out and directly insert it into the cutting medium, with an insertion depth of 5cm;

[0128] Seedbed management: After cutting, a shade net with a shading rate of 70% should be placed 1m above the seedbed. The humidity of the seedbed should be controlled at 65% and the temperature at 20℃.

[0129] Transplantation: 40 days after cutting, transplant the cuttings with intact roots into a pre-sterilized mixed substrate of perlite, peat soil, and sand (1.5:1.5:0.5). The substrate should be sterilized with 0.1% potassium permanganate solution before use and watered immediately after transplantation.

[0130] Example 8

[0131] Preparation of cuttings: Select healthy and pest-free adult plants, select semi-lignified, complete terminal buds, intact leaves, 0.6 cm in diameter, and about 25 cm in length from the south of the plant. Ring cut the branches 2 cm away from the main branches at the base, with a width of 0.4 cm. After ring cutting, use a blade to gently peel off the bark and scrape off the cambium to ensure that the cut is smooth and flat, and then wrap it with 3 cm wide black tape for 2 circles for light protection. After callus tissue is formed at the ring cutting site, cut the branches 1 cm below the ring cutting site, remove the black tape, wrap the moisturizing tape with a wet towel and bring it back to the nursery for use;

[0132] Cutting medium: Use a mixture of perlite and vermiculite in a ratio of 4:1 as the cutting medium;

[0133] Cutting treatment: Cut off all leaves of the cuttings collected from the wild, and keep a maximum of 5 buds on each cutting. Cut the upper incision flat and the lower incision oblique. Soak the lower end of the cutting in a solution of 1200ml / L indolebutyric acid for 30 minutes, take it out and directly insert it into the cutting medium, with an insertion depth of 6cm;

[0134] Seedbed management: After cutting, a shade net with a shading rate of 70% should be placed 1m above the seedbed. The humidity of the seedbed should be controlled at 65% and the temperature at 20℃.

[0135] Transplantation: 40 days after cutting, transplant the cuttings with intact roots into a pre-sterilized mixed substrate of perlite, peat soil, and sand (1.5:1.5:0.5). The substrate should be sterilized with 0.1% potassium permanganate solution before use and watered immediately after transplantation.

[0136] Example 9

[0137] Preparation of cuttings: Select healthy and pest-free adult plants, select semi-lignified, complete terminal buds, intact leaves, 0.6 cm in diameter, and about 25 cm in length from the south of the plant. Ring cut the branches 2 cm away from the main branches at the base, with a width of 0.4 cm. After ring cutting, use a blade to gently peel off the bark and scrape off the cambium to ensure that the cut is smooth and flat, and then wrap it with 3 cm wide black tape for 2 circles for light protection. After callus tissue is formed at the ring cutting site, cut the branches 1 cm below the ring cutting site, remove the black tape, wrap the moisturizing tape with a wet towel and bring it back to the nursery for use;

[0138] Cutting medium: Use a mixture of perlite and vermiculite in a ratio of 4:1 as the cutting medium;

[0139] Cutting treatment: Cut off all leaves of the cuttings collected from the wild, and keep a maximum of 4 buds on each cutting. Cut the upper incision flat and the lower incision oblique. Soak the lower end of the cutting in a 1000ml / L indolebutyric acid solution for 40 minutes, take it out and directly insert it into the cutting medium, with an insertion depth of 5cm;

[0140] Seedbed management: After cutting, a shade net with a shading rate of 75% should be used to cover the seedbed 2m above the seedbed. The humidity of the seedbed should be controlled at 70% and the temperature at 23℃.

[0141] Transplantation: 40 days after cutting, transplant the cuttings with intact roots into a pre-sterilized mixed substrate of perlite, peat soil, and sand (1.5:1.5:0.5). The substrate should be sterilized with 0.1% potassium permanganate solution before use and watered immediately after transplantation.

[0142] Example 10

[0143] Preparation of cuttings: Select healthy and pest-free adult plants, select semi-lignified, complete terminal buds, intact leaves, 0.6 cm in diameter, and about 25 cm in length from the south of the plant. Ring cut the branches 2 cm away from the main branches at the base, with a width of 0.4 cm. After ring cutting, use a blade to gently peel off the bark and scrape off the cambium to ensure that the cut is smooth and flat, and then wrap it with 3 cm wide black tape for 2 circles for light protection. After callus tissue is formed at the ring cutting site, cut the branches 1 cm below the ring cutting site, remove the black tape, wrap the moisturizing tape with a wet towel and bring it back to the nursery for use;

[0144] Cutting medium: Use a mixture of perlite and vermiculite in a ratio of 4:1 as the cutting medium;

[0145] Cutting treatment: Cut off all leaves of the cuttings collected from the wild, and keep a maximum of 4 buds on each cutting. Cut the upper incision flat and the lower incision oblique. Soak the lower end of the cutting in a 1000ml / L indolebutyric acid solution for 40 minutes, take it out and directly insert it into the cutting medium, with an insertion depth of 5cm;

[0146] Seedbed management: After cutting, the seedbed should be covered with a shade net with a shading rate of 80% at 3m above the seedbed. The humidity of the seedbed should be controlled at 80% and the temperature at 26℃.

[0147] Transplantation: 40 days after cutting, transplant the cuttings with intact roots into a pre-sterilized mixed substrate of perlite, peat soil, and sand (1.5:1.5:0.5). The substrate should be sterilized with 0.1% potassium permanganate solution before use and watered immediately after transplantation.

[0148] Embodiment 11

[0149] Preparation of cuttings: Select healthy and pest-free adult plants, select semi-lignified, complete terminal buds, intact leaves, 0.6 cm in diameter, and about 25 cm in length from the south of the plant. Ring cut the branches 2 cm away from the main branches at the base, with a width of 0.4 cm. After ring cutting, use a blade to gently peel off the bark and scrape off the cambium to ensure that the cut is smooth and flat, and then wrap it with 3 cm wide black tape for 2 circles for light protection. After callus tissue is formed at the ring cutting site, cut the branches 1 cm below the ring cutting site, remove the black tape, wrap the moisturizing tape with a wet towel and bring it back to the nursery for use;

[0150] Cutting medium: Use a mixture of perlite and vermiculite in a ratio of 4:1 as the cutting medium;

[0151] Cutting treatment: Cut off all leaves of the cuttings collected from the wild, and keep a maximum of 4 buds on each cutting. Cut the upper incision flat and the lower incision oblique. Soak the lower end of the cutting in a 1000ml / L indolebutyric acid solution for 40 minutes, take it out and directly insert it into the cutting medium, with an insertion depth of 5cm;

[0152] Seedbed management: After cutting, a shade net with a shading rate of 75% should be used to cover the seedbed 2m above the seedbed. The humidity of the seedbed should be controlled at 70% and the temperature at 23℃.

[0153] Transplantation: 40 days after cutting, transplant the cuttings with intact roots into a pre-sterilized mixed substrate of perlite, peat soil and sand in a ratio of 2:2:1. The substrate should be sterilized with 0.1% potassium permanganate solution before use and watered immediately after transplantation.

[0154] Example 12

[0155] Preparation of cuttings: Select healthy and pest-free adult plants, select semi-lignified, complete terminal buds, intact leaves, 0.6 cm in diameter, and about 25 cm in length from the south of the plant. Ring cut the branches 2 cm away from the main branches at the base, with a width of 0.4 cm. After ring cutting, use a blade to gently peel off the bark and scrape off the cambium to ensure that the cut is smooth and flat, and then wrap it with 3 cm wide black tape for 2 circles for light protection. After callus tissue is formed at the ring cutting site, cut the branches 1 cm below the ring cutting site, remove the black tape, wrap the moisturizing tape with a wet towel and bring it back to the nursery for use;

[0156] Cutting medium: Use a mixture of perlite and vermiculite in a ratio of 4:1 as the cutting medium;

[0157] Cutting treatment: Cut off all leaves of the cuttings collected from the wild, and keep a maximum of 5 buds on each cutting. Cut the upper incision flat and the lower incision oblique. Soak the lower end of the cutting in a solution of 1200ml / L indolebutyric acid for 30 minutes, take it out and directly insert it into the cutting medium, with an insertion depth of 6cm;

[0158] Seedbed management: After cutting, a shade net with a shading rate of 75% should be used to cover the seedbed 2m above the seedbed. The humidity of the seedbed should be controlled at 70% and the temperature at 23℃.

[0159] Transplantation: 40 days after cutting, transplant the cuttings with intact roots into a pre-sterilized mixed substrate of perlite, peat soil and sand in a ratio of 3:3:2. The substrate should be sterilized with 0.1% potassium permanganate solution before use and watered immediately after transplantation.

[0160] Embodiment 13

[0161] Preparation of cuttings: Select healthy and pest-free adult plants, select semi-lignified, complete terminal buds, intact leaves, 0.6 cm in diameter, and about 25 cm in length from the south of the plant. Ring cut the branches 2 cm away from the main branches at the base, with a width of 0.4 cm. After ring cutting, use a blade to gently peel off the bark and scrape off the cambium to ensure that the cut is smooth and flat, and then wrap it with 3 cm wide black tape for 2 circles for light protection. After callus tissue is formed at the ring cutting site, cut the branches 1 cm below the ring cutting site, remove the black tape, wrap the moisturizing tape with a wet towel and bring it back to the nursery for use;

[0162] Cutting medium: Use a mixture of perlite and vermiculite in a ratio of 4:1 as the cutting medium;

[0163] Cutting treatment: Cut off all leaves of the cuttings collected from the wild, and keep each cutting 12±0.2cm long, retain at most 4 buds, cut the upper incision flat, and cut the lower incision oblique. Soak the lower end of the cutting in a 1000ml / L indolebutyric acid solution for 40 minutes, take it out and place it in a cutting jig, insert it into the cutting medium through the cutting jig, and insert it to a depth of 5cm;

[0164] Seedbed management: After cutting, a shade net with a shading rate of 75% should be used to cover the seedbed 2m above the seedbed. The humidity of the seedbed should be controlled at 70% and the temperature at 23℃.

[0165] Transplantation: 40 days after cutting, transplant the cuttings with intact roots into a pre-sterilized mixed substrate of perlite, peat soil and sand in a ratio of 2:2:1. The substrate should be sterilized with 0.1% potassium permanganate solution before use and watered immediately after transplantation.

[0166] Embodiment 14

[0167] Preparation of cuttings: Select healthy and pest-free adult plants, select semi-lignified, complete terminal buds, intact leaves, 0.6 cm in diameter, and about 25 cm in length from the south of the plant. Ring cut the branches 2 cm away from the main branches at the base, with a width of 0.4 cm. After ring cutting, use a blade to gently peel off the bark and scrape off the cambium to ensure that the cut is smooth and flat, and then wrap it with 3 cm wide black tape for 2 circles for light protection. After callus tissue is formed at the ring cutting site, cut the branches 1 cm below the ring cutting site, remove the black tape, wrap the moisturizing tape with a wet towel and bring it back to the nursery for use;

[0168] Cutting medium: Use a mixture of perlite and vermiculite in a ratio of 4:1 as the cutting medium;

[0169] Cutting treatment: Cut off all leaves of the cuttings collected from the wild, and keep each cutting 12±0.2cm long, retain at most 4 buds, cut the upper incision flat, and cut the lower incision oblique. Soak the lower end of the cutting in a 1000ml / L indolebutyric acid solution for 40 minutes, take it out and place it in a cutting jig, insert it into the cutting medium through the cutting jig, and insert it to a depth of 5cm;

[0170] Seedbed management: After cutting, a shade net with a shading rate of 75% should be used to cover the seedbed 2m above the seedbed. The humidity of the seedbed should be controlled at 70% and the temperature at 23℃.

[0171] Transplantation: 40 days after cutting, transplant the cuttings with intact roots into a pre-sterilized mixed matrix of perlite, peat soil and sand in a ratio of 2:2:1. The matrix should be sterilized with 0.1% potassium permanganate solution before use, and watered immediately after transplantation. Then, 3 holes should be drilled in the mixed matrix, which should be set around and near the branches of Boluo red beans, and fermented soybean milk should be poured into the holes at a volume of 5% of the matrix volume.

[0172] Embodiment 15

[0173] Preparation of cuttings: Select healthy and pest-free adult plants, select semi-lignified, complete terminal buds, intact leaves, 0.6 cm in diameter, and about 25 cm in length from the south of the plant. Ring cut the branches 2 cm away from the main branches at the base, with a width of 0.4 cm. After ring cutting, use a blade to gently peel off the bark and scrape off the cambium to ensure that the cut is smooth and flat, and then wrap it with 3 cm wide black tape for 2 circles for light protection. After callus tissue is formed at the ring cutting site, cut the branches 1 cm below the ring cutting site, remove the black tape, wrap the moisturizing tape with a wet towel and bring it back to the nursery for use;

[0174] Cutting medium: Use a mixture of perlite and vermiculite in a ratio of 4:1 as the cutting medium;

[0175] Cutting treatment: Cut off all leaves of the cuttings collected from the wild, and keep each cutting 12±0.2cm long, retain at most 4 buds, cut the upper incision flat, and cut the lower incision oblique. Soak the lower end of the cutting in a 1000ml / L indolebutyric acid solution for 40 minutes, take it out and place it in a cutting jig, insert it into the cutting medium through the cutting jig, and insert it to a depth of 5cm;

[0176] Seedbed management: After cutting, a shade net with a shading rate of 75% should be used to cover the seedbed 2m above the seedbed. The humidity of the seedbed should be controlled at 70% and the temperature at 23℃.

[0177] Transplantation: 40 days after cutting, transplant the cuttings with intact roots into a pre-sterilized mixed matrix of perlite, peat soil and sand in a ratio of 2:2:1. The matrix should be disinfected with 0.1% potassium permanganate solution before use, and watered immediately after transplantation. Then, 3 holes should be drilled in the mixed matrix, which should be set around and near the branches of Boluo red beans, and fermented soybean milk should be poured into the holes at a volume of 8% of the matrix volume.

[0178] Example 16

[0179] Preparation of cuttings: Select healthy and pest-free adult plants, select semi-lignified, complete terminal buds, intact leaves, 0.6 cm in diameter, and about 25 cm in length from the south of the plant. Ring cut the branches 2 cm away from the main branches at the base, with a width of 0.4 cm. After ring cutting, use a blade to gently peel off the bark and scrape off the cambium to ensure that the cut is smooth and flat, and then wrap it with 3 cm wide black tape for 2 circles for light protection. After callus tissue is formed at the ring cutting site, cut the branches 1 cm below the ring cutting site, remove the black tape, wrap the moisturizing tape with a wet towel and bring it back to the nursery for use;

[0180] Cutting medium: Use a mixture of perlite and vermiculite in a ratio of 4:1 as the cutting medium;

[0181] Cutting treatment: Cut off all leaves of the cuttings collected from the wild, and keep each cutting 12±0.2cm long, retain at most 4 buds, cut the upper incision flat, and cut the lower incision oblique. Soak the lower end of the cutting in a 1000ml / L indolebutyric acid solution for 40 minutes, take it out and place it in a cutting jig, insert it into the cutting medium through the cutting jig, and insert it to a depth of 5cm;

[0182] Seedbed management: After cutting, a shade net with a shading rate of 75% should be used to cover the seedbed 2m above the seedbed. The humidity of the seedbed should be controlled at 70% and the temperature at 23℃.

[0183] Transplantation: 40 days after cutting, transplant the root-complete cuttings to a pre-sterilized mixed matrix of perlite, peat soil, and sand in a ratio of 2:2:1. The matrix should be sterilized with 0.1% potassium permanganate solution before use, and watered immediately after transplantation. Then, 3 holes should be drilled in the mixed matrix, which should be set around and near the branches of Boluo red beans, and fermented soybean milk should be poured into the holes, with the amount used being 10% of the volume of the matrix.

[0184] Comparative Example

[0185] Preparation of cuttings: Select healthy and pest-free adult plants, select semi-lignified, complete terminal buds, intact leaves, 0.6 cm in diameter, and about 25 cm in length from the south of the plant. Cut the branches at the base of the branches 1 cm away from the main branches, wrap them with a wet towel to keep them moist, and bring them back to the nursery for later use;

[0186] Cutting medium: Use a mixture of perlite and vermiculite in a ratio of 4:1 as the cutting medium;

[0187] Cutting treatment: Cut off all leaves of the cuttings collected from the wild, and keep a maximum of 4 buds on each cutting. Cut the upper incision flat and the lower incision oblique. Soak the lower end of the cutting in a 1000ml / L indolebutyric acid solution for 40 minutes, take it out and directly insert it into the cutting medium, with an insertion depth of 5cm;

[0188] Seedbed management: After cutting, a shade net with a shading rate of 75% should be used to cover the seedbed 2m above the seedbed. The humidity of the seedbed should be controlled at 70% and the temperature at 23℃.

[0189] Transplantation: Cuttings (40 days, plus an additional 30 days for callus formation), after 70 days, transplant the rooted cuttings to a pre-sterilized perlite, peat, sand mixed substrate of 2:2:1. The substrate was sterilized with 0.1% potassium permanganate solution before use, and watered immediately after transplanting.

[0190] It should be noted that the number of Boluo Red Bean branches used for cuttings in each of the above embodiments and comparative examples is 100. The rooting rate and survival rate of the Boluo Red Bean branches in each embodiment and comparative example are recorded below, and the data are shown in Table 1:

[0191] Table 1: Rooting rate and survival rate of Boluo red bean branches (data are average values)

[0192]

[0193]

[0194] As can be seen from Table 1, the callus formation rate of the branches of the undetached Boluo red bean plants was significantly higher than that of the branches of the detached Boluo red bean plants, and the rooting rate of the formed callus was significantly higher, and the survival rate was significantly higher, especially the rooting rate and survival rate of the Boluo red bean branches using the cutting jig were higher, indicating that the callus of the Boluo red bean branches using the cutting jig had better integrity and better differentiation ability. In addition, the survival rate of the Boluo red bean branches of Examples 14 to 16 was as high as 100%, indicating that watering with fermented soy milk can effectively improve the survival rate of the Boluo red bean branches. Figure 5As shown, a is pruning the Boluo red bean plant to obtain the Boluo red bean branch just cut from the Boluo red bean plant, b is pruning the branches of the Boluo red bean branch, c is the obtained Boluo red bean branch with callus tissue at the ring-shaped incision; d is cutting the Boluo red bean branch into the substrate, e is the rooting growth of Boluo red bean after being planted in the substrate, and f is the survival and growth after being transplanted into the mixed substrate.

[0195] The above embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for those of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A method for propagating Boluo red beans, characterized in that: The steps include: Obtaining a Boluo Red Bean branch, wherein the Boluo Red Bean branch is a side branch growing on a Boluo Red Bean main branch; Performing a girdling operation on the Boluo Red Bean branch, so that a circular incision is formed in the circumference of the Boluo Red Bean branch, and the circular incision is peeled off to the wood part; The annular incision is subjected to light shielding treatment so that callus tissue is formed at the annular incision; The shading-treated red bean branches are removed from the tree; The Boluo red bean branches after the tree removal treatment are transplanted.

2. The method for propagating Boluo red beans according to claim 1, characterized in that: The Boluo red bean branches are semi-lignified and south-oriented, and are strong branches of the current year with complete terminal buds and intact leaves.

3. The method for breeding Boluo red beans according to claim 1, characterized in that: The diameter of the Boluo red bean branch is 0.5cm-0.7cm, and the length is about 20cm-30cm.

4. The method for propagating Boluo red beans according to claim 1, characterized in that: The annular incision is formed at the base of the Boluo red bean branch.

5. The method for propagating Boluo red beans according to claim 1, characterized in that: The width of the annular incision is 0.3 cm to 0.5 cm.

6. The method for propagating Boluo red beans according to claim 1, characterized in that: The annular incision is subjected to a light shielding process, which specifically comprises the following steps: Performing a wrapping and covering process on the annular incision so as to place the annular incision in a light-shielding environment; The annular incision after the winding and covering treatment is subjected to a generation treatment so as to generate callus tissue at the annular incision.

7. The method for propagating Boluo red beans according to claim 6, characterized in that: The annular incision after the wrapping and covering process is subjected to a generation process, and the process time is 20 days to 30 days.

8. The method for propagating Boluo red beans according to claim 1, characterized in that: The shading-treated Boluo red bean branches are subjected to tree removal treatment, and the specific steps are as follows: Pruning is performed at the base of the Boluo red bean branch and between the annular incision and the main branch to separate the Boluo red bean branch from the main branch.

9. The method for propagating Boluo red beans according to claim 1, characterized in that: The Boluo red bean branches after the tree removal treatment are transplanted, which specifically comprises the following steps: The base of the Boluo red bean branch is moistened to make the annular incision moist environment; Carrying out planting treatment on the Boluo red bean branches after the pruning treatment; The Boluo red bean branches after the pruning of branches and leaves are subjected to a planting treatment so that the Boluo red bean branches are cut into a substrate.

10. The Boluo red bean propagation method according to claim 9, characterized in that: The matrix includes perlite and vermiculite.

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