Detection system and method based on anaerobic ammonia oxidation sludge

Abstract

The invention provides a detection system and method based on anaerobic ammonia oxidation sludge, and relates to an anaerobic ammonia oxidation biological denitrification technology for industrial wastewater, municipal sewage and the like, the detection system comprises a biological gas production detection module, a laser-induced fluorescence detection module and a data acquisition and processing module; the biological gas production detection module comprises a constant-temperature water bath unit, a fermentation reaction unit, a gas drying unit, a gas production metering unit and a single-chip microcomputer data acquisition and processing unit; the fermentation reaction unit is provided with a feeding hole and a liquid outlet. The fermentation reaction unit is communicated with the gas drying unit through a gas guide pipe, the gas drying unit is communicated with the produced gas metering unit through a gas guide pipe, and the single-chip microcomputer data acquisition and processing unit is respectively in circuit connection with the fermentation reaction unit, the gas drying unit and the produced gas metering unit; the laser-induced fluorescence detection module is communicated with the fermentation reaction unit through a liquid outlet. The problem that an existing activity determination method for detecting the activity of the anaerobic ammonia oxidation sludge is low in efficiency is solved.

Description

Technical Field

[0001] The present application relates to anaerobic ammonium oxidation biological denitrification technology for industrial wastewater, municipal sewage, etc., and in particular to a detection system and method based on anaerobic ammonium oxidation sludge. Background Art

[0002] At present, anaerobic ammonium oxidation is a microbial reaction that produces nitrogen gas and some nitrates under anaerobic conditions, using ammonia as an electron donor and nitrite as an electron acceptor. Compared with the traditional nitrification-denitrification denitrification process, the anaerobic process based on this reaction has significant advantages such as low oxygen consumption, no need for external organic carbon source and sludge emission reduction. It has been recognized as one of the most economical biological denitrification processes. Anaerobic ammonium oxidation mainly transforms ammonia through the anaerobic ammonium oxidizing bacteria in the anaerobic ammonium oxidation sludge. The activity of the anaerobic ammonium oxidation sludge is the key to the successful operation of the anaerobic ammonium oxidation process. For this reason, the rapid perception of changes in the activity of the anaerobic ammonium oxidation sludge and the optimization and adjustment of the process operating parameters in combination with the influent load and water quality characteristics are the key to achieving long-term stable operation of the above process.

[0003] In the prior art, due to the slow growth rate of anaerobic ammonium-oxidizing bacteria, the strictly anaerobic growth environment, and the susceptibility to inhibition by external factors, there are many challenges in the stable operation of the anaerobic ammonium-oxidizing process. The growth characteristics of anaerobic ammonium-oxidizing sludge make it difficult to control and apply it in the engineering operation process. There is currently no specific online activity detection method, and it is impossible to use the conventional activated sludge activity identification method to judge. The commonly used activity determination methods are not directly tested in the culture environment, resulting in a time lag between the measured activity and the actual activity, and the detection time is long, requiring long-term operation, which is easy to cause data distortion and increase the detection cost. That is, the existing activity determination method cannot quickly detect the activity of anaerobic ammonium-oxidizing sludge, and the detection efficiency is low. Summary of the invention

[0004] The present application provides a detection system and method based on anaerobic ammonium oxidation sludge, which is used to solve the technical problem that the existing activity determination methods are inefficient in detecting the activity of anaerobic ammonium oxidation sludge.

[0005] In a first aspect, the present application provides a detection system based on anaerobic ammonium oxidation sludge, the detection system comprising a biogas production detection module, a laser induced fluorescence detection module, and a data acquisition and processing module;

[0006] The biogas production detection module includes a constant temperature water bath unit, a fermentation reaction unit, a gas drying unit, a gas production metering unit and a single chip computer data acquisition and processing unit; the fermentation reaction unit has a feed inlet and a liquid outlet;

[0007] The fermentation reaction unit, the gas drying unit and the gas production metering unit are located in the constant temperature water bath unit; the fermentation reaction unit and the gas drying unit are connected via an air duct, the gas drying unit and the gas production metering unit are connected via an air duct, the single-chip computer data acquisition and processing unit are respectively connected in circuit with the fermentation reaction unit, the gas drying unit and the gas production metering unit; the laser induced fluorescence detection module is connected in circuit with the fermentation reaction unit via the liquid outlet, and the data acquisition and processing module is respectively connected in circuit with the single-chip computer data acquisition and processing unit and the laser induced fluorescence detection module;

[0008] The single-chip computer data acquisition and processing unit is used to control the fermentation reaction unit to ferment the initial mixed liquid entering through the feed inlet to generate fermented gas, and transport the gas to the gas drying unit; control the gas drying unit to dry the gas and transport the dried gas to the gas production metering unit; control the gas production metering unit to determine the gas pressure value of the gas;

[0009] The laser induced fluorescence detection module is used to obtain the fermented mixed liquid through the liquid outlet, perform laser fluorescence detection on the fermented mixed liquid, and obtain fluorescence intensity data; wherein the fluorescence intensity data represents the growth information of the anaerobic ammonia-oxidizing bacteria in the mixed liquid during the fermentation process;

[0010] The data acquisition and processing module is used to obtain the air pressure value sent by the single-chip data acquisition and processing unit and the fluorescence intensity data sent by the laser induced fluorescence detection module; and determine the activity index of the anaerobic ammonia oxidation sludge in the mixed liquid according to the air pressure value and the fluorescence intensity data.

[0011] Further, the determining of the activity index of the anaerobic ammonium oxidation sludge in the mixed liquid according to the air pressure value and the fluorescence intensity data includes:

[0012] Determine the linear fitting slope value of the gas production during the fermentation process and the linear fitting slope value of the protein-like concentration in the fermented mixed liquid according to the gas pressure value and the fluorescence intensity data;

[0013] Determine the linear fitting slope value of the activity of the anaerobic ammonium oxidation sludge according to a preset slope formula, the linear fitting slope value of the gas production, and the linear fitting slope value of the protein-like concentration;

[0014] Determining a target activity level corresponding to the linear fitting slope value of the activity of the anaerobic ammonium oxidation sludge according to a preset mapping relationship between the linear fitting slope value and the activity level;

[0015] Determine the linear fitting slope value of the activity of the anaerobic ammonium oxidation sludge, the target activity level, and the gas production, which are all activity indicators of the anaerobic ammonium oxidation sludge in the mixed liquid;

[0016] The slope formula is as follows:

[0017] When K Nx ≥K N , M=K Nx / K N *R; when K Nx ≤K N , M=-K N / K Nx *R;

[0018] Wherein, M represents the linear fitting slope value of the activity of the anaerobic ammonium oxidation sludge and the activity index of the anaerobic ammonium oxidation sludge; R is a preset correction coefficient; K N is the baseline linear slope of gas production in anammox sludge, K Nx is the linear slope of the gas production in the anaerobic ammonium-oxidizing granular sludge to be measured;

[0019] The preset correction coefficient R is the linear fitting slope ratio between the protein concentration and the gas production of the anaerobic ammonium oxidation sludge during the fermentation process. The calculation formula of the correction coefficient R is as follows:

[0020] When K Nx ≥K N When R = 1-|(K Qx / K Q -K Nx / K N ) / (K Qx / K Q )|;

[0021] When K Nx ≤KN, R=1-|(K Q / K Qx -K N / K Nx ) / ( / K Q / K Qx )|;K Q Indicates the reference value of fluorescence intensity detection, K Qx Represents the fluorescence intensity of the protein-like substance to be detected.

[0022] Further, the constant temperature water bath unit includes a constant temperature water bath and a temperature sensor located in the constant temperature water bath;

[0023] The fermentation reaction unit includes a reaction tank, a first sealing cover arranged on the upper part of the reaction tank, a stepping motor, a coupling, a stirring rod, a stirring blade; a feed sealing ball valve, a pneumatic throttle valve, a three-way solenoid valve, a first straight solenoid valve, a second straight solenoid valve, a third straight solenoid valve, a discharge sealing ball valve, and a coarse filter membrane;

[0024] A feed inlet is provided at the upper part of one side of the reaction tank, and the feed inlet is connected to the liquid outlet of the feed sealing ball valve through a first liquid inlet pipe; the liquid inlet of the feed sealing ball valve is communicated with the configured mixed liquid container through a second liquid inlet pipe;

[0025] A circulating liquid outlet and a circulating liquid inlet are provided on one side of the reaction tank, and the positions of the circulating liquid outlet and the circulating liquid inlet are both lower than the feed inlet; coarse filter membranes are provided at the outlets of the circulating liquid outlet and the circulating liquid inlet, and the circulating liquid outlet is connected to the first straight electromagnetic valve through the first circulating liquid pipe, and the circulating liquid inlet is connected to the second straight electromagnetic valve through the second circulating liquid pipe;

[0026] A discharge port is provided at a position close to the bottom of the reaction tank and away from the circulating liquid outlet, and the discharge port is connected to a discharge sealing ball valve through an eighth air guide pipe.

[0027] Furthermore, an aeration air inlet is provided on one side of the upper portion of the first sealing cover, and the aeration air inlet is connected to the air outlet of the pneumatic throttle valve through a first air guide pipe; the air inlet of the pneumatic throttle valve is connected to the inert gas cylinder through a second air guide pipe;

[0028] An air outlet is provided on the other side of the upper part of the first sealing cover, and the air outlet is connected to the air inlet of the three-way solenoid valve through the fifth air duct. The first air outlet of the three-way solenoid valve is connected to the atmospheric environment through the sixth air duct, and the second air outlet of the three-way solenoid valve is connected to the gas drying unit through the seventh air duct.

[0029] Furthermore, a three-way joint is connected to the first air duct, a first interface of the three-way joint is connected to a second interface of a third direct solenoid valve through a third air duct, and a third interface of the third direct solenoid valve is connected to a fourth interface of the gas drying unit through a fourth air duct.

[0030] Further, the gas drying unit includes a U-shaped tube, a sealing plug located at the upper part of the U-shaped tube, a layered sheet, and a gas drying area and an indication area obtained by dividing the layered sheet; one side of the gas drying unit is connected to the gas production metering unit through a ninth gas guide pipe;

[0031] The gas production metering unit includes a gas collecting bottle and a second sealing cover arranged on the upper part of the gas collecting bottle, and a high-precision pressure transmitter is installed on the upper part of the second sealing cover; a gas outlet on one side of the gas collecting bottle is connected to the gas inlet of the exhaust solenoid valve through the tenth air duct, and the gas outlet of the exhaust solenoid valve is connected to the atmospheric environment through the eleventh air duct; the exhaust solenoid valve is provided with a preset pressure value for exhaust.

[0032] Further, the data acquisition and processing module includes a personal computer;

[0033] The single chip data acquisition processing unit is used to obtain the air pressure value of the gas through the high-precision pressure transmitter and send the air pressure value to the personal computer;

[0034] The personal computer is used to obtain the air pressure value and the fluorescence intensity data sent by the laser induced fluorescence detection module.

[0035] Further, the laser induced fluorescence detection module includes a circulating liquid outlet unit, a laser induced fluorescence detection unit, and a laser induced fluorescence detection control unit;

[0036] The circulating liquid outlet unit and the laser induced fluorescence detection unit are separated by a preset light projection distance, and the laser induced fluorescence detection unit is circuit-connected to the laser induced fluorescence detection control unit;

[0037] The laser induced fluorescence detection control unit is used to control the circulating liquid outlet unit to circulate and obtain the fermented mixed liquid; control the laser induced fluorescence detection unit to perform laser fluorescence detection on the fermented mixed liquid in the circulating liquid outlet unit to obtain fluorescence intensity data.

[0038] Furthermore, the circulating liquid outlet unit includes a precision peristaltic pump, a one-way valve, a fine filter with a preset concentration value, and a liquid pool;

[0039] The liquid inlet of the precision peristaltic pump is connected to the outlet of the first straight electromagnetic valve through the third circulating liquid pipe; the liquid outlet of the precision peristaltic pump, the one-way valve, the fine filter, and the liquid pool are connected in series through the fourth circulating liquid pipe, the fifth circulating liquid pipe, and the sixth circulating liquid pipe; the liquid pool is connected to the liquid inlet of the second straight electromagnetic valve through the seventh circulating liquid pipe;

[0040] The laser induced fluorescence detection unit includes a laser light source, a laser filter, a spectroscope, a fluorescence filter, and a photodetector;

[0041] The laser-induced fluorescence detection control unit comprises a microcontroller, and the microcontroller controls the precision peristaltic pump and the laser-induced fluorescence detection unit and performs data processing.

[0042] In a second aspect, the present application provides a detection method based on an anaerobic ammonium oxidation sludge detection system, wherein the detection system includes a biogas production detection module, a laser induced fluorescence detection module, and a data acquisition and processing module; applied to the data acquisition and processing module, the method includes:

[0043] In the process of fermenting and gasifying the mixed liquid by the biological gas production detection module, obtaining the gas pressure value of the fermented gas;

[0044] Performing laser-induced fluorescence detection on the mixed liquid in the fermentation process by the laser-induced fluorescence detection module to obtain fluorescence intensity data;

[0045] Determining the activity index of the anaerobic ammonium oxidation sludge in the mixed liquid according to the air pressure value and the fluorescence intensity data;

[0046] Wherein, the detection system is the system as described in the first aspect.

[0047] The present application provides a detection system and method based on anaerobic ammonia oxidation sludge, wherein the detection system includes a biological gas production detection module, a laser induced fluorescence detection module, and a data acquisition and processing module; wherein the biological gas production detection module includes a constant temperature water bath unit, a fermentation reaction unit, a gas drying unit, a gas production metering unit, and a single-chip data acquisition and processing unit; the fermentation reaction unit has a feed inlet and a liquid outlet. The fermentation reaction unit, the gas drying unit, and the gas production metering unit are located in the constant temperature water bath unit; the fermentation reaction unit is connected to the gas drying unit through an air duct, the gas drying unit is connected to the gas production metering unit through an air duct, and the single-chip data acquisition and processing unit is respectively connected to the fermentation reaction unit, the gas drying unit, and the gas production metering unit; the laser induced fluorescence detection module is connected to the fermentation reaction unit through the liquid outlet, and the data acquisition and processing module is respectively connected to the single-chip data acquisition and processing unit and the laser induced fluorescence detection module. The single-chip microcomputer data acquisition and processing unit is used to control the fermentation reaction unit to ferment the initial mixed liquid entering through the feed port, generate fermented gas, and transport the gas to the gas drying unit; control the gas drying unit to dry the gas and transport the dried gas to the gas production metering unit; control the gas production metering unit to determine the gas pressure value. The laser induced fluorescence detection module is used to obtain the fermented mixed liquid through the liquid outlet, perform laser fluorescence detection on the fermented mixed liquid, and obtain fluorescence intensity data; wherein the fluorescence intensity data characterizes the growth information of the anaerobic ammonia-oxidizing bacteria in the mixed liquid during the fermentation process. The data acquisition and processing module is used to obtain the air pressure value sent by the single-chip microcomputer data acquisition and processing unit and the fluorescence intensity data sent by the laser induced fluorescence detection module; and determine the activity index of the anaerobic ammonia-oxidizing sludge in the mixed liquid based on the air pressure value and the fluorescence intensity data. In this scheme, the pressure value of the fermented gas is obtained through the single-chip data acquisition and processing unit, and the pressure value is sent to the data acquisition and processing module; the laser induced fluorescence detection module performs laser fluorescence detection on the fermented mixed liquid in real time to obtain fluorescence intensity data, and the fluorescence intensity data is sent to the data acquisition and processing module; the data acquisition and processing module couples the pressure value and the fluorescence intensity data to calculate the activity index of the anaerobic ammonium oxidation sludge in the mixed liquid. Therefore, without destroying the sludge structure, the intelligent real-time and rapid determination of the activity index is realized, solving the technical problem of low efficiency of the existing activity determination method in detecting the activity of anaerobic ammonium oxidation sludge. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present disclosure and, together with the description, serve to explain the principles of the present disclosure.

[0049] Figure 1A schematic diagram of the overall structure of an anaerobic ammonium oxidation sludge detection system provided in an embodiment of the present application;

[0050] Figure 2 A schematic diagram of the structure of a fermentation reaction unit provided in an embodiment of the present application.

[0051] Description of reference numerals: 1-biological gas production detection module; 11-constant temperature water bath unit; 111-constant temperature water bath pot; 112-temperature sensor; 12-fermentation reaction unit; 121-reaction tank; 122-first sealing cover; 123-feeding port; 124-first liquid inlet pipe; 125-feeding sealing ball valve; 126-second liquid inlet pipe; 127-circulating liquid outlet; 128-circulating liquid inlet; 129-coarse filtration membrane; 1210-first circulating liquid pipe; 1211-second circulating liquid pipe; 1212-first straight-through solenoid valve; 1213-second straight-through solenoid valve; 12 14-motor connector; 1215-stepping motor; 1216-coupling; 1217-stirring rod; 1218-stirring blade; 1219-aeration air inlet; 1220-first air pipe; 1221-pneumatic throttle valve; 1222-second air pipe; 1223-third air pipe; 1224-third direct electromagnetic valve; 1225-fourth air pipe; 1226-air outlet; 1227-fifth air pipe; 1228-three-way electromagnetic valve; 1229-sixth air pipe; 1230-seventh air pipe; 1231-discharge port; 1232-eighth air pipe; 1 233-discharge sealing ball valve; 13-gas drying unit; 131-U-shaped tube; 132-sealing plug; 133-layered sheet; 134-granular desiccant; 135-color-changing silica gel indicator; 136-ninth air pipe; 14-gas production metering unit; 141-gas collecting bottle; 142-second sealing cover; 143-high-precision pressure transmitter; 144-tenth air pipe; 145-exhaust solenoid valve; 146-eleventh air pipe; 15-single-chip computer data acquisition and processing unit; 16-atmospheric pressure sensor; 2-laser-induced fluorescence detection module; 21-circulating liquid outlet unit; 211-precision peristaltic pump; 212-one-way valve; 213-fine filter; 214-liquid pool; 215-third circulating liquid pipe; 216-fourth circulating liquid pipe; 217-fifth circulating liquid pipe; 218-sixth circulating liquid pipe; 219-seventh circulating liquid pipe; 22-laser induced fluorescence detection unit; 221-laser light source; 222-laser filter; 223-beam splitter; 224-fluorescence filter; 225-photodetector; 23-laser induced fluorescence detection control unit; 231-microcontroller; 3-data acquisition and processing module; 31-personal computer.

[0052] The above drawings show clear embodiments of the present disclosure, which will be described in more detail below. These drawings and text descriptions are not intended to limit the scope of the present disclosure in any way, but to illustrate the concepts of the present disclosure to those skilled in the art by referring to specific embodiments. DETAILED DESCRIPTION

[0053] Here, exemplary embodiments will be described in detail, examples of which are shown in the accompanying drawings. When the following description refers to the drawings, unless otherwise indicated, the same numbers in different drawings represent the same or similar elements. The implementations described in the following exemplary embodiments do not represent all implementations consistent with the present disclosure.

[0054] At present, anaerobic ammonium oxidation is a microbial reaction that produces nitrogen gas and some nitrates under anaerobic conditions, using ammonia as an electron donor and nitrite as an electron acceptor. Compared with the traditional nitrification-denitrification denitrification process, the anaerobic process based on this reaction has significant advantages such as low oxygen consumption, no need for external organic carbon source and sludge emission reduction. It has been recognized as one of the most economical biological denitrification processes. Anaerobic ammonium oxidation mainly transforms ammonia through the anaerobic ammonium oxidizing bacteria in the anaerobic ammonium oxidation sludge. The activity of the anaerobic ammonium oxidation sludge is the key to the successful operation of the anaerobic ammonium oxidation process. For this reason, the rapid perception of changes in the activity of the anaerobic ammonium oxidation sludge and the optimization and adjustment of the process operating parameters in combination with the influent load and water quality characteristics are the key to achieving long-term stable operation of the above process.

[0055] In one example, due to the slow growth rate of anaerobic ammonium-oxidizing bacteria, the strictly anaerobic growth environment, and susceptibility to inhibition by external factors, there are many challenges in the stable operation of the anaerobic ammonium-oxidizing process. The growth characteristics of anaerobic ammonium-oxidizing sludge make it difficult to control and apply it during engineering operation. There is currently no specific online activity detection method, and it is impossible to use conventional activated sludge activity identification methods to judge. Currently, the commonly used activity determination methods are not directly tested in the culture environment, resulting in a time lag between the measured activity and the actual activity, and the detection time is long, requiring long-term operation, which is easy to cause data distortion and increase detection costs. That is, the existing activity determination methods cannot quickly detect the activity of anaerobic ammonium-oxidizing sludge, and the detection efficiency is low.

[0056] In addition, there is an essential difference between anaerobic ammonium oxidation sludge and activated sludge. Activated sludge is generally a biochemical treatment under aerobic conditions, while anaerobic processes require anoxic or anaerobic conditions. In addition, there are essential differences between anaerobic ammonium oxidation sludge and activated sludge in terms of treatment effect, operational complexity, and adaptability to the environment. The commonly used monitoring methods and means for activated sludge cannot be applied to anaerobic ammonium oxidation sludge. In summary, rapid determination of anaerobic ammonium oxidation sludge activity and anaerobic ammonium oxidation process engineering are one of the core technical issues that need to be solved urgently.

[0057] The present application provides a detection system and method based on anaerobic ammonium oxidation sludge, which aims to solve the above technical problems of the prior art.

[0058] The technical solution of the present application and how the technical solution of the present application solves the above-mentioned technical problems are described in detail below with specific embodiments. The following specific embodiments can be combined with each other, and the same or similar concepts or processes may not be repeated in some embodiments. The embodiments of the present application will be described below in conjunction with the accompanying drawings.

[0059] Figure 1 The overall structural diagram of an anaerobic ammonium oxidation sludge detection system provided in the embodiment of the present application is as follows: Figure 1 As shown, the detection system includes a biological gas production detection module, a laser induced fluorescence detection module, and a data acquisition and processing module; wherein the biological gas production detection module includes a constant temperature water bath unit, a fermentation reaction unit, a gas drying unit, a gas production metering unit and a single-chip microcomputer data acquisition and processing unit; the fermentation reaction unit has a feed inlet and a liquid outlet.

[0060] The fermentation reaction unit, the gas drying unit and the gas production metering unit are located in the constant temperature water bath unit; the fermentation reaction unit and the gas drying unit are connected through an air duct, the gas drying unit and the gas production metering unit are connected through an air duct, and the single-chip computer data acquisition and processing unit is respectively connected to the fermentation reaction unit, the gas drying unit and the gas production metering unit by circuits; the laser induced fluorescence detection module is connected to the fermentation reaction unit through a liquid outlet, and the data acquisition and processing module is respectively connected to the single-chip computer data acquisition and processing unit and the laser induced fluorescence detection module by circuits.

[0061] The single-chip microcomputer data acquisition and processing unit is used to control the fermentation reaction unit to ferment the initial mixed liquid entering through the feed port, generate fermented gas, and transport the gas to the gas drying unit; control the gas drying unit to dry the gas and transport the dried gas to the gas production metering unit; control the gas production metering unit to determine the gas pressure value.

[0062] The laser induced fluorescence detection module is used to obtain the fermented mixed liquid through the liquid outlet, perform laser fluorescence detection on the fermented mixed liquid, and obtain fluorescence intensity data; wherein the fluorescence intensity data represents the growth information of the anaerobic ammonia-oxidizing bacteria in the mixed liquid during the fermentation process.

[0063] The data acquisition and processing module is used to obtain the air pressure value sent by the single-chip data acquisition and processing unit and the fluorescence intensity data sent by the laser-induced fluorescence detection module; and determine the activity index of the anaerobic ammonia oxidation sludge in the mixed liquid according to the air pressure value and the fluorescence intensity data.

[0064] For example, Figure 1 As shown, the detection system includes a biological gas production detection module 1, a laser induced fluorescence detection module 2, and a data acquisition and processing module 3. Among them, the biological gas production detection module 1 includes a constant temperature water bath unit 11, a fermentation reaction unit 12, a gas drying unit 13, a gas production metering unit 14 and a single-chip data acquisition and processing unit 15; the biological gas production detection module 1 also includes an atmospheric pressure sensor 16, and the fermentation reaction unit 12 has a feed inlet and a liquid outlet.

[0065] The fermentation reaction unit 12, the gas drying unit 13 and the gas production metering unit 14 are all located in the constant temperature water bath unit 11; the fermentation reaction unit 12 and the gas drying unit 13 are connected via an air duct, and the gas drying unit 13 and the gas production metering unit 14 are connected via an air duct; the single-chip computer data acquisition and processing unit 15 is respectively connected to the constant temperature water bath unit 11, the fermentation reaction unit 12, the gas drying unit 13 and the gas production metering unit 14 by circuits; the laser induced fluorescence detection module 2 is connected to the fermentation reaction unit 12 via a liquid outlet, the data acquisition and processing module 3 is connected to the single-chip computer data acquisition and processing unit 15 by circuits, and the data acquisition and processing module 3 is connected to the laser induced fluorescence detection module 2 by circuits.

[0066] The single-chip data acquisition and processing unit 15 is used to control the constant temperature water bath unit 11, the fermentation reaction unit 12, the gas drying unit 13, and the gas production metering unit 14 to perform corresponding operations. Specifically, the single-chip data acquisition and processing unit 15 can control the temperature of the constant temperature water bath unit 11 to maintain a preset temperature value; control the fermentation reaction unit 12 to ferment the initial mixed liquid entering through the feed inlet, generate fermented gas, and transport the gas to the gas drying unit 13; control the gas drying unit 13 to dry the gas and transport the dried gas to the gas production metering unit 14; control the gas production metering unit 14 to determine the gas pressure value of the gas.

[0067] The laser induced fluorescence detection module 2 is used to obtain the fermented mixed liquid through the liquid outlet during the fermentation process, perform laser fluorescence detection on the fermented mixed liquid, and obtain fluorescence intensity data; wherein the fluorescence intensity data represents the growth information of the anaerobic ammonium oxidizing bacteria community in the mixed liquid during the fermentation process, and the anaerobic ammonium oxidizing bacteria community is related to the activity of the anaerobic ammonium oxidizing sludge in the mixed liquid.

[0068] The data acquisition and processing module 3 is used to obtain the air pressure value sent by the single-chip data acquisition and processing unit 15 and the fluorescence intensity data sent by the laser induced fluorescence detection module 2; and determine the activity index of the anaerobic ammonia oxidation sludge in the mixed liquid according to the air pressure value and the fluorescence intensity data.

[0069] In the embodiment of the present application, the detection system includes a biological gas production detection module, a laser induced fluorescence detection module, and a data acquisition and processing module; wherein the biological gas production detection module includes a constant temperature water bath unit, a fermentation reaction unit, a gas drying unit, a gas production metering unit, and a single-chip data acquisition and processing unit; the fermentation reaction unit has a feed inlet and a liquid outlet. The fermentation reaction unit, the gas drying unit, and the gas production metering unit are located in the constant temperature water bath unit; the fermentation reaction unit and the gas drying unit are connected through an air duct, and the gas drying unit and the gas production metering unit are connected through an air duct, and the single-chip data acquisition and processing unit is respectively connected to the fermentation reaction unit, the gas drying unit, and the gas production metering unit; the laser induced fluorescence detection module is connected to the fermentation reaction unit through the liquid outlet, and the data acquisition and processing module is respectively connected to the single-chip data acquisition and processing unit and the laser induced fluorescence detection module. The single-chip microcomputer data acquisition and processing unit is used to control the fermentation reaction unit to ferment the initial mixed liquid entering through the feed port, generate fermented gas, and transport the gas to the gas drying unit; control the gas drying unit to dry the gas and transport the dried gas to the gas production metering unit; control the gas production metering unit to determine the gas pressure value. The laser induced fluorescence detection module is used to obtain the fermented mixed liquid through the liquid outlet, perform laser fluorescence detection on the fermented mixed liquid, and obtain fluorescence intensity data; wherein the fluorescence intensity data characterizes the growth information of the anaerobic ammonia-oxidizing bacteria in the mixed liquid during the fermentation process. The data acquisition and processing module is used to obtain the air pressure value sent by the single-chip microcomputer data acquisition and processing unit and the fluorescence intensity data sent by the laser induced fluorescence detection module; and determine the activity index of the anaerobic ammonia-oxidizing sludge in the mixed liquid based on the air pressure value and the fluorescence intensity data. In this scheme, the pressure value of the fermented gas is obtained through the single-chip data acquisition and processing unit, and the pressure value is sent to the data acquisition and processing module; the laser induced fluorescence detection module performs laser fluorescence detection on the fermented mixed liquid in real time to obtain fluorescence intensity data, and the fluorescence intensity data is sent to the data acquisition and processing module; the data acquisition and processing module couples the pressure value and the fluorescence intensity data to calculate the activity index of the anaerobic ammonium oxidation sludge in the mixed liquid. Therefore, the intelligent real-time and rapid determination of the activity index is realized, solving the technical problem of low efficiency of the existing activity determination method in detecting the activity of anaerobic ammonium oxidation sludge.

[0070] In one example, the activity index of the anaerobic ammonium oxidation sludge in the mixed liquid is determined according to the air pressure value and the fluorescence intensity data, including: determining the linear fitting slope value of the gas production in the fermentation process and the linear fitting slope value of the protein-like concentration in the fermented mixed liquid according to the air pressure value and the fluorescence intensity data; determining the linear fitting slope value of the activity of the anaerobic ammonium oxidation sludge according to a preset slope formula, the linear fitting slope value of the gas production, and the linear fitting slope value of the protein-like concentration; determining the target activity level corresponding to the linear fitting slope value of the activity of the anaerobic ammonium oxidation sludge according to the mapping relationship between the preset linear fitting slope value and the activity level; determining the linear fitting slope value of the activity of the anaerobic ammonium oxidation sludge, the target activity level, and the gas production, which are all activity indicators of the anaerobic ammonium oxidation sludge in the mixed liquid;

[0071] The slope formula is as follows:

[0072] When K Nx ≥K N , M=K Nx / K N *R; when K Nx ≤K N , M=-K N / K Nx *R;

[0073] Wherein, M represents the linear fitting slope value of the activity of the anaerobic ammonium oxidation sludge and the activity index of the anaerobic ammonium oxidation sludge; R is a preset correction coefficient; K N is the baseline linear slope of gas production in anammox sludge, K Nx is the linear slope of the gas production in the anaerobic ammonium-oxidizing granular sludge to be measured;

[0074] The preset correction coefficient R is the linear fitting slope ratio between the protein concentration and the gas production of the anaerobic ammonium oxidation sludge during the fermentation process. The calculation formula of the correction coefficient R is as follows:

[0075] When K Nx ≥K N When R = 1-|(K Qx / K Q -K Nx / K N ) / (K Qx / K Q )|;

[0076] When K Nx ≤KN, R=1-|(K Q / K Qx -K N / K Nx ) / ( / K Q / K Qx )|;KQ Indicates the reference value of fluorescence intensity detection, K Qx Represents the fluorescence intensity of the protein-like substance to be detected.

[0077] Exemplarily, in the process of determining the activity index of the anaerobic ammonium oxidation sludge in the mixed liquid, the data acquisition and processing module 3 comprehensively considers the sludge functional effect, gas production rate, effluent dissolved organic matter and other indicators to perform a semi-quantitative evaluation of the sludge, and uses the linear fitting slope of the N2 gas production per unit time and the protein-like concentration in the effluent dissolved organic matter obtained by the metabolism of the oxygen-ammonium oxidation granular sludge per unit time as the judgment of the activity level of the anaerobic ammonium oxidation sludge, and the calculation formula is as follows:

[0078] Anaerobic ammonium oxidation granular sludge activity M = K Nx / K N *R(K Nx ≥K N ), M=-K N / K Nx *R(K Nx ≤K N ), where R is the correction factor, K Nx and K N are the slopes of gas production, K N is the baseline linear slope of gas production in anammox granular sludge, K Nx is the linear slope of the gas production in the anaerobic ammonium oxidation granular sludge to be tested, and M is the linear fitting slope value of the activity of the anaerobic ammonium oxidation sludge.

[0079] Through the data of activity M, the activity index of anaerobic ammonia oxidation granular sludge is pre-defined into multiple levels, that is, the sludge in inactivated, unstable, healthy and rapidly reproducing states is divided into multiple activity levels, specifically level L1, level L2, level L3, level L4, level L5, level L6, level L7, level L8, level L9, level L10, etc., and there is no limitation on this.

[0080] Based on the mapping relationship between the preset linear fitting slope value and the activity level, a correction coefficient based on the linear fitting slope value of the gas production volume and the linear fitting slope value of the protein-like concentration during the fermentation process is determined, and the activity index of the anaerobic ammonium oxidation sludge in the fermented mixed liquor is calculated. For example, when M < -10, the sludge is inactive; when -10 ≤ M < -8, the target activity level of the anaerobic ammonium oxidation granular sludge activity is the L1 level; when -8 ≤ M < -6, the target activity level of the sludge activity is the L2 level; when -6 ≤ M < -4, the target activity level of the sludge activity is the L3 level; when -4 ≤ M < -2, the target activity level of the sludge activity is the L4 level; when -2 ≤ M ≤ -1 or 1 ≤ M ≤ 2, the target activity level of the sludge activity is the L5 level; when 2 < M ≤ 4, the target activity level of the sludge activity is the L6 level; when 4 < M ≤ 6, the target activity level of the sludge activity is the L7 level; when 6 < M ≤ 8, the target activity level of the sludge activity is the L8 level; when 8 < M ≤ 10, the target activity level of the sludge activity is the L9 level; when M > 10, the target activity level of the sludge activity is the L10 level.

[0081] Taking the detection data of the anaerobic ammonium oxidation granular sludge under the normal load state after domestication engineered by the applicant as the preset evaluation standard rule information, M = 1 or -1, corresponding to the anaerobic ammonium oxidation granular sludge activity level L5.

[0082] Among them, as Figure 1 shown, the measurement methods of K Nx and K N : Through the precise measurement of the pressure change, the gas production mass of N2, N = PV0 / RT, is calculated, where P is obtained by detecting with the high-precision pressure transmitter 143, V0 is the volume of the gas measurement system, and R is the preset well-known molar gas constant. The N2 gas production mass N at each time point Ti within 10 - 30 min of the L5 anaerobic ammonium oxidation granular sludge is measured i (i = 1…n, n ≥ 10), i is the number of detections; according to the measured n N i and Ti, a linear equation is fitted to obtain the slope K N ; the anaerobic ammonium oxidation granular sludge to be tested is taken, and the N2 mass N at each time point Ti within 10 - 30 min is measured xi (i = 1…n, n ≥ 10); according to the measured n N xi and Ti, a linear equation is fitted to obtain the slope K Nx .

[0083] The correction coefficient is measured by the ratio of the fluorescence intensity of protein-like to the linear fitting slope of the nitrogen gas production volume. When K Nx ≥ K N , R = 1 - |(K Qx / K Q - K Nx / K N ) / (K Qx / K Q )|; When K Nx ≤K N When R = 1-|(K Q / K Qx -K N / K Nx ) / ( / K Q / K Qx )|. K Q Indicates the reference value of fluorescence intensity detection, K Qx It represents the fluorescence intensity of the protein-like substance to be tested. The protein-like fluorescence intensity is obtained by detecting the protein-like fluorescence concentration of the dissolved organic matter in the liquid pool 214 after the metabolism of the anaerobic ammonium oxidation granular sludge by the laser induced fluorescence detection module 2. Under the condition of a certain sludge concentration, the fluorescence intensity of the microbial extracellular matrix is ​​related to the concentration of the group, which is linearly proportional to the activity of the microorganism (or the growth rate of the microorganism). That is, under the same microbial activity, the concentration of the microbial extracellular matrix will increase with the metabolism of the microorganism, showing an increase in fluorescence intensity. Therefore, K Qx and K Q The measurement method is: detect the fluorescence intensity Qi (i = 1...n, n ≥ 5) of the protein-like substance in L5 anaerobic ammonium oxidation sludge at each time point i within 10-30 minutes. By fitting multiple groups of fluorescence intensity data, the linear slope K of the protein-like fluorescence intensity of L5 anaerobic ammonium oxidation sludge is obtained. Q Take the anaerobic ammonium oxidation granular sludge to be tested, and measure the fluorescence intensity Qxi (i=1…n, n≥5) of the protein-like substance at each time point i within 10-30min; fit the measured n Qxi into a linear equation to obtain the slope K Qx .

[0084] In the embodiment of the present application, the linear fitting slope value of the gas production in the fermentation process and the linear fitting slope value of the protein-like concentration in the fermented mixed liquid are determined according to the air pressure value and the fluorescence intensity data; the linear fitting slope value of the activity of the anaerobic ammonium oxidation sludge is determined according to the preset slope formula, the linear fitting slope value of the gas production, and the linear fitting slope value of the protein-like concentration; according to the mapping relationship between the preset linear fitting slope value and the activity level, the target activity level corresponding to the linear fitting slope value of the activity of the anaerobic ammonium oxidation sludge is determined; the linear fitting slope value of the activity of the anaerobic ammonium oxidation sludge, the target activity level, and the gas production are all activity indicators of the anaerobic ammonium oxidation sludge in the mixed liquid. Therefore, by detecting the gas production rate of anaerobic ammonium oxidation granular sludge and the concentration of dissolved organic matter in the effluent after its metabolism, an activity grade system is established by utilizing the internal correlation characteristics between the two and the sludge activity. The activity of anaerobic ammonium oxidation sludge is graded according to the activity grade system, and the activity indicators of anaerobic ammonium oxidation sludge are quickly and intelligently identified. The activity indicators of anaerobic ammonium oxidation sludge can be quickly and semi-quantitatively determined without destroying the sludge particles and microbial structure. The measuring device is easy to operate and is suitable for a variety of engineering scenarios. It solves the technical problem of low efficiency of existing activity determination methods in detecting the activity of anaerobic ammonium oxidation sludge.

[0085] In an example, Figure 1 As shown, the constant temperature water bath unit includes a constant temperature water bath pot and a temperature sensor located in the constant temperature water bath pot.

[0086] For example, Figure 1 As shown, the constant temperature water bath unit 11 includes a constant temperature water bath pot 111 and a temperature sensor 112 installed in the constant temperature water bath pot 111. The fermentation reaction unit 12, the gas drying unit 13, and the gas production metering unit 14 are all located in the constant temperature water bath unit 11. The fermentation reaction unit 12, the gas drying unit 13, and the gas production metering unit 14 are connected in sequence through an air duct.

[0087] In the embodiment of the present application, the constant temperature water bath unit includes a constant temperature water bath and a temperature sensor located in the constant temperature water bath. Therefore, the constant temperature water bath and the temperature sensor ensure that the fermentation reaction and the gas production measurement are both carried out in a set constant temperature environment.

[0088] In one example, Figure 2 A schematic diagram of the structure of a fermentation reaction unit provided in an embodiment of the present application is shown in FIG. Figure 2 As shown, the fermentation reaction unit includes a reaction tank, a first sealing cover arranged on the upper part of the reaction tank, a stepper motor, a coupling, a stirring rod, a stirring blade; a feed sealing ball valve, a pneumatic throttle valve, a three-way solenoid valve, a first straight-through solenoid valve, a second straight-through solenoid valve, a third straight-through solenoid valve, a discharge sealing ball valve, and a coarse filter membrane.

[0089] A feed port is arranged on the upper part of one side of the reaction tank, and the feed port is connected to the liquid outlet of the feed sealing ball valve through a first liquid inlet pipe; the liquid inlet of the feed sealing ball valve is communicated with the configured mixed liquid container through a second liquid inlet pipe.

[0090] A circulating liquid outlet and a circulating liquid inlet are arranged on one side of the reaction tank, and the positions of the circulating liquid outlet and the circulating liquid inlet are both lower than the feed inlet; coarse filter membranes are arranged at the outlets of both the circulating liquid outlet and the circulating liquid inlet, and the circulating liquid outlet is connected to the first straight-through solenoid valve through the first circulating liquid pipe, and the circulating liquid inlet is connected to the second straight-through solenoid valve through the second circulating liquid pipe.

[0091] A discharge port is arranged at a position close to the bottom of the reaction tank and away from the circulating liquid outlet, and the discharge port is connected to the discharge sealing ball valve through the eighth air guide pipe.

[0092] For example, Figure 2 As shown, the fermentation reaction unit 12 includes a reaction tank 121, a first sealing cover 122 arranged on the upper part of the reaction tank 121, a stepper motor 1215, a coupling 1216, a stirring rod 1217, and a stirring blade 1218 connected to the stirring rod 1217; a feed sealing ball valve 125, a pneumatic throttle valve 1221, a three-way solenoid valve 1228, a first straight-through solenoid valve 1212, a second straight-through solenoid valve 1213, a third straight-through solenoid valve 1224, a discharge sealing ball valve 1233, a coarse filter membrane 129, an air guide pipe, and a liquid pipe, etc.; wherein, the three-way solenoid valve 1228 includes "air inlet 0", "first air outlet 1", and "second air outlet 2", and these three ports are only the identification of the ports of the three-way solenoid valve 1228, which are different from the component identification 1 and 2 in the accompanying drawing description.

[0093] The reaction tank 121 and the first sealing cover 122 disposed on the upper part of the reaction tank 121 provide a place for the anaerobic ammonia-oxidizing bacteria fermentation reaction. The upper part of the first sealing cover 122 is connected to the stepper motor 1215 through the motor connector 1214, and the motor output shaft of the stepper motor 1215 is connected to the stirring rod 1217 through the coupling 1216, and the end of the stirring rod 1217 is connected to the stirring blade 1218. Therefore, the stepper motor 1215 provides stirring power by rotating during operation, driving the stirring rod 1217 and the stirring blade 1218 to stir the mixed liquid.

[0094] A feed port 123 is provided at the upper part of one side of the reaction tank 121, and the feed port 123 is used for feeding the mixed liquid; the feed port 123 is connected to the liquid outlet of the feed sealing ball valve 125 through the first liquid inlet pipe 124, and the liquid inlet of the feed sealing ball valve 125 is communicated with the configured mixed liquid container through the second liquid inlet pipe 126. Therefore, the mixed liquid in the mixed liquid container can be transported to the reaction tank 121 through the mixed liquid container, the second liquid inlet pipe 126, the feed sealing ball valve 125, the first liquid inlet pipe 124 and the feed port 123.

[0095] A circulating liquid outlet 127 and a circulating liquid inlet 128 are provided on one side of the reaction tank, and the positions of the circulating liquid outlet 127 and the circulating liquid inlet 128 are lower than the feed inlet 123; a coarse filter membrane 129 is provided at the outlets of both the circulating liquid outlet 127 and the circulating liquid inlet 128, and the circulating liquid outlet 127 is connected to the first straight-through solenoid valve 1212 through the first circulating liquid pipe 1210, and the circulating liquid inlet 128 is connected to the second straight-through solenoid valve 1213 through the second circulating liquid pipe 1211; wherein, the first straight-through solenoid valve 1212 and the second straight-through solenoid valve 1213 are opened and closed simultaneously during the detection process, and when the laser induced fluorescence detection module 2 is not working, both are in a closed state. Therefore, the fermented mixed liquid required for detection can be circulated to the laser induced fluorescence detection module 2 through the circulating liquid outlet 127 and the circulating liquid inlet 128, and during the circulation acquisition process, the fermented mixed liquid is coarsely filtered through the coarse filter membrane 129 set at the outlets of the circulating liquid outlet 127 and the circulating liquid inlet 128 to prevent granular sludge from flowing out of the reaction tank 121.

[0096] A discharge port 1231 is provided at a position close to the bottom of the reaction tank 121 and away from the circulating liquid outlet 127 . The discharge port 1231 is connected to a discharge sealing ball valve 1233 through an eighth air guide pipe 1232 .

[0097] In an example, Figure 2 As shown, an aeration air inlet is provided on one side of the upper portion of the first sealing cover, and the aeration air inlet is connected to the air outlet of the pneumatic throttle valve through a first air guide pipe; the air inlet of the pneumatic throttle valve is connected to the inert gas cylinder through a second air guide pipe.

[0098] An air outlet is provided on the other side of the upper part of the first sealing cover, and the air outlet is connected to the air inlet of the three-way solenoid valve through the fifth air duct. The first air outlet of the three-way solenoid valve is connected to the atmospheric environment through the sixth air duct. The second air outlet of the three-way solenoid valve is connected to the gas drying unit through the seventh air duct.

[0099] For example, Figure 2As shown, an aeration air inlet 1219 is provided on one side of the upper portion of the first sealing cover, and the aeration air inlet 1219 is connected to the air outlet of the pneumatic throttle valve 1221 through the first air guide pipe 1220; the air inlet of the pneumatic throttle valve 1221 is connected to the inert gas cylinder through the second air guide pipe 1222, and the inert gas cylinder can be an inert gas cylinder such as nitrogen or argon, and there is no limitation to this; wherein, the pneumatic throttle valve 1221 is used to control the flow rate of aeration.

[0100] An air outlet 1226 is provided on the other side of the upper part of the first sealing cover 122, and the air outlet 1226 is connected to the "air inlet 0" of the three-way solenoid valve 1228 through the fifth air duct 1227, the "first air outlet 1" of the three-way solenoid valve 1228 is connected to the atmospheric environment through the sixth air duct 1229, and the "second air outlet 2" of the three-way solenoid valve 1228 is connected to the gas drying unit 13 through the seventh air duct 1230.

[0101] In an example, Figure 2 As shown, a three-way joint is connected to the first air duct, the first interface of the three-way joint is connected to the second interface of the third direct solenoid valve through the third air duct, and the third interface of the third direct solenoid valve is connected to the fourth interface of the gas drying unit through the fourth air duct.

[0102] For example, Figure 2 As shown, a three-way joint is connected to the first air duct 1220, and the first interface A in the three-way joint is connected to the second interface B of the third direct solenoid valve 1224 through the third air duct 1223, and the third interface C of the third direct solenoid valve 1224 is connected to the fourth interface D of the gas drying unit 13 through the fourth air duct 1225.

[0103] In an example, Figure 1 As shown, the gas drying unit includes a U-shaped tube, a sealing plug located at the upper part of the U-shaped tube, a layered sheet, and a gas drying area and an indication area obtained by dividing the layered sheet; one side of the gas drying unit is connected to the gas production metering unit through a ninth gas guide pipe.

[0104] The gas production metering unit includes a gas collecting bottle and a second sealing cover arranged on the upper part of the gas collecting bottle, and a high-precision pressure transmitter is installed on the upper part of the second sealing cover; a gas outlet on one side of the gas collecting bottle is connected to the gas inlet of the exhaust solenoid valve through the tenth gas duct, and the gas outlet of the exhaust solenoid valve is connected to the atmospheric environment through the eleventh gas duct; the exhaust solenoid valve is provided with a preset pressure value for exhaust.

[0105] For example, Figure 1As shown, the gas drying unit 13 includes a U-shaped tube 131, a sealing plug 132 located at the upper part of the U-shaped tube, a layered sheet 133 located in the middle part of the U-shaped tube, and a gas drying area and an indication area obtained by segmentation based on the layered sheet; one side of the gas drying unit 13 is connected to the gas production metering unit 14 through the ninth gas guide pipe 136. Among them, the gas drying area in the U-shaped tube 131 is filled with a suitable granular desiccant 134, and the indication area is filled with a suitable color-changing silica gel indicator 135. Therefore, the gas drying unit 13 can further absorb moisture from the biogas through the gas drying area, and indicate the dryness of the gas through the color-changing silica gel indicator. When the color-changing silica gel indicator changes from blue to red, it indicates that a new desiccant needs to be replaced, and then the gas drying unit 13 can eliminate the influence of water vapor pressure on the metering result. In addition, by setting a certain pressure relief, the selection range of the pressure transmitter is greatly reduced, thereby improving its measurement accuracy, so that the measurement result is closer to the actual N2 production value.

[0106] The gas production metering unit 14 includes a gas collecting bottle 141 and a second sealing cover 142 arranged on the upper part of the gas collecting bottle. A high-precision pressure transmitter 143 is installed on the upper part of the second sealing cover 142, and the high-precision pressure transmitter 143 is used to detect the change of gas production pressure; the gas outlet on one side of the gas collecting bottle is connected to the gas inlet of the exhaust solenoid valve 145 through the tenth gas guide tube 144, and the gas outlet of the exhaust solenoid valve 145 is connected to the atmospheric environment through the eleventh gas guide tube 146, or the biogas discharged from the eleventh gas guide tube 146 can be collected by a gas collecting bag to further analyze the composition of the biogas; the exhaust solenoid valve 145 is provided with a preset pressure value for exhaust, that is, when the pressure value of the high-precision pressure transmitter 143 reaches the preset pressure value, the exhaust solenoid valve 145 opens quickly, and closes quickly after reaching the set time period, which greatly reduces the selection range of the pressure transmitter, thereby improving the measurement accuracy.

[0107] Furthermore, the reaction tank 121, the U-shaped tube 131, and the gas collecting bottle 141 are all sealed high-pressure resistant glass bottles, and there is sealing silicone grease evenly applied at the joints of their respective sealing covers, thereby improving the sealing of the reaction tank 121, the U-shaped tube 131, and the gas collecting bottle 141, thereby ensuring the accuracy of the activity index.

[0108] Furthermore, the volume V0 of the gas measurement system is the total volume of the biogas distribution, including the sum of the headspace volume of the U-tube 131, the volume of the gas guide tube and the volume of the gas collecting bottle 141; the gas collecting bottle 141 is usually a standard gas collecting bottle of 50 to 100 mL, and there is no limitation on this.

[0109] In an example, Figure 1As shown, the data acquisition and processing module includes a personal computer; a single-chip data acquisition and processing unit, which is used to obtain the gas pressure value through a high-precision pressure transmitter and send the gas pressure value to the personal computer; the personal computer is used to obtain the gas pressure value and the fluorescence intensity data sent by the laser induced fluorescence detection module.

[0110] For example, Figure 1 As shown, the data acquisition and processing module 3 includes a personal computer (PC) 31, and also includes the data acquisition and output parts of the single-chip data acquisition and processing unit 15 and the laser induced fluorescence detection control unit 23. The single-chip data acquisition and processing unit 15 can obtain the gas pressure value of the gas through the high-precision pressure transmitter 143, and send the gas pressure value to the personal computer 31. The personal computer 31 obtains the gas pressure value and the fluorescence intensity data sent by the laser induced fluorescence detection module 2.

[0111] The single-chip data acquisition processing unit 15 can control the biological gas production detection module 1. Specifically, the single-chip data acquisition processing unit 15 is responsible for controlling the temperature sensor 112, the stepper motor 1215, the three-way solenoid valve 1228, the first straight solenoid valve 1212, the second straight solenoid valve 1213, the third straight solenoid valve 1224, and the exhaust solenoid valve 145, and collecting biological gas production data, which includes the air pressure value collected by the high-precision pressure transmitter 143 and the atmospheric pressure value collected by the atmospheric pressure sensor 16. Finally, the biological gas production data is processed and input into the personal computer 31, so that the personal computer 31 couples the biological gas production data and the fluorescence intensity data sent by the laser induced fluorescence detection module 2, and further comprehensively determines the activity index of the anaerobic ammonia oxidation sludge.

[0112] In an example, Figure 1 As shown, the laser induced fluorescence detection module includes a circulating liquid outlet unit, a laser induced fluorescence detection unit, and a laser induced fluorescence detection control unit; the circulating liquid outlet unit and the laser induced fluorescence detection unit are separated by a preset light projection distance, and the laser induced fluorescence detection unit and the laser induced fluorescence detection control unit are circuit-connected.

[0113] The laser induced fluorescence detection control unit is used to control the circulating liquid outlet unit to circulate and obtain the fermented mixed liquid; control the laser induced fluorescence detection unit to perform laser fluorescence detection on the fermented mixed liquid in the circulating liquid outlet unit to obtain fluorescence intensity data.

[0114] For example, Figure 1As shown, the laser induced fluorescence detection module 2 includes a circulating liquid outlet unit 21, a laser induced fluorescence detection unit 22, and a laser induced fluorescence detection control unit 23. The circulating liquid outlet unit 21 and the laser induced fluorescence detection unit 22 are separated by a preset light projection distance, and the laser induced fluorescence detection unit 22 and the laser induced fluorescence detection control unit 23 are connected by a circuit.

[0115] The laser induced fluorescence detection control unit 23 can control the circulating liquid outlet unit 21 and the laser induced fluorescence detection unit 22 to realize data collection and data transmission. Specifically, the laser induced fluorescence detection control unit 23 can control the circulating liquid outlet unit 21 to circulate and obtain the fermented mixed liquid, and control the laser induced fluorescence detection unit 22 to perform laser fluorescence detection on the fermented mixed liquid in the circulating liquid outlet unit 21 to obtain fluorescence intensity data.

[0116] In an example, Figure 1 As shown, the circulating liquid outlet unit includes a precision peristaltic pump, a one-way valve, a fine filter with a preset concentration value, and a liquid pool.

[0117] Among them, the liquid inlet of the precision peristaltic pump is connected to the outlet of the first straight electromagnetic valve through the third circulating liquid pipe; the liquid outlet of the precision peristaltic pump, the one-way valve, the fine filter, and the liquid pool are connected in series through the fourth circulating liquid pipe, the fifth circulating liquid pipe, and the sixth circulating liquid pipe; the liquid pool is connected to the liquid inlet of the second straight electromagnetic valve through the seventh circulating liquid pipe. The laser induced fluorescence detection unit includes a laser light source, a laser filter, a spectroscope, a fluorescence filter, and a photodetector. The laser induced fluorescence detection control unit includes a microcontroller, which controls the precision peristaltic pump and the laser induced fluorescence detection unit 22 and processes data.

[0118] For example, Figure 1 As shown, the circulating liquid outlet unit 21 includes a precision peristaltic pump 211, a one-way valve 212, a fine filter 213 with a preset concentration value, and a liquid pool 214. Among them, the liquid inlet of the precision peristaltic pump 211 is connected to the outlet of the first straight electromagnetic valve 1212 through the third circulating liquid pipe 215; the liquid outlet of the precision peristaltic pump 211 is connected to the one-way valve 212 through the fourth circulating liquid pipe 216, the one-way valve 212 is connected to the fine filter 213 through the fifth circulating liquid pipe 217, the fine filter 213 is connected to the liquid pool 214 through the sixth circulating liquid pipe 218; the liquid pool 214 is connected to the liquid inlet of the second straight electromagnetic valve 1213 through the seventh circulating liquid pipe 219.

[0119] The laser induced fluorescence detection unit 22 includes a laser light source 221, a laser filter 222, a beam splitter 223, a fluorescence filter 224, and a photodetector 225. The laser induced fluorescence detection control unit 23 includes a microcontroller 231, and the microcontroller 231 controls and processes data for the precision peristaltic pump 211 and the laser induced fluorescence detection unit 22. For example, the laser wavelength is 300 to 400 nm, the laser output power is 30 to 80 mW, and the fluorescence spectrum range is 250 to 450 nm, which is not limited.

[0120] Therefore, the laser induced fluorescence detection control unit can control the circulating liquid outlet unit 21 to circulate the fermented mixed liquid through the liquid inlet of the precision peristaltic pump 211, and output the fermented mixed liquid through the liquid outlet of the precision peristaltic pump 211. At this time, the fermented mixed liquid passes through the one-way valve 212 and the fine filter 213 in sequence, and is finally stored in the liquid pool 214. Then the laser induced fluorescence detection unit 22 is controlled to perform laser fluorescence detection on the fermented mixed liquid in the liquid pool 214 to obtain fluorescence intensity data. The fermented mixed liquid in the liquid pool 214 is transported to the reaction tank 121 through the liquid inlet of the second straight-through solenoid valve 1213 connected to the seventh circulating liquid pipe 219.

[0121] Therefore, a vacuum feeding design is adopted to strictly keep the entire system closed and the total volume of biogas production constant; the fermented mixed liquid in the liquid pool 214 is measured by a laser induced fluorescence spectrometer, and the measurement speed is fast. In combination with the present application, the fluorescence intensity data of the protein concentration after metabolism of the anaerobic ammonia oxidation granular sludge can be measured in real time and non-contactly, without the need for multiple destructive pretreatments of the test substances. Without destroying the sludge particles and microbial structure, the purpose of rapid semi-quantitative determination of the activity of the anaerobic ammonia oxidation sludge can be achieved. The measuring device is easy to operate, suitable for a variety of engineering scenarios, and is convenient and fast.

[0122] In one example, the sensing material of the high-precision pressure transmitter 143 is a ceramic diaphragm sensor, the total accuracy can reach within 0.1%, and the range can be selected from -10 to 25 Kpa, which is not limited.

[0123] Based on the above embodiments, the detection method of the detection system for determining the activity index of anaerobic ammonium oxidation sludge is described in detail. The detection process is as follows:

[0124] (1): Prepare a mixed liquid of anaerobic ammonium oxidation granular sludge, simulated wastewater, nutrient solution and other materials, and mix the anaerobic ammonium oxidation granular sludge, simulated wastewater and nutrient solution evenly.

[0125] Specifically, the simulated wastewater consists of NO2 - -N and NH4 +-N; the nutrient solution consists of Fe (0 valent), AlCl3, CaCl2, MgSO4·7H2O, KH2PO4, and NaHCO3.

[0126] The mixture liquid is composed of anaerobic ammonium oxidation granular sludge, simulated wastewater, nutrient solution and other materials. Specifically, the added mass concentration of anaerobic ammonium oxidation granular sludge is within a preset concentration range, for example, the preset concentration range is 60-90g / L, which is not limited; NO2 - -N and NH4 + -N concentration ratio is 1.0-1.2, NH4 + -N concentration range can be 60-200mg / L, which is not limited; the composition of the nutrient solution is Fe (0 valent) 5-8mg / L, AlCl3 0.025g / L-0.1g / L, CaCl2 0.1-0.27g / L, MgSO4·7H2O0.1-0.3g / L, KH2PO4 0.01-0.015g / L, NaHCO3 0.5g / L-0.84g / L, etc. Among them, the Fe element can directly affect the activity of key enzymes in anaerobic ammonia-oxidizing bacteria, increase the heme content, strengthen the transfer rate of extracellular matrix and nutrients, and promote the improvement of microbial cell activity. Al can effectively promote the granulation process of sludge, and Ca and Mg are necessary elements for microbial metabolism and growth.

[0127] (2): Check the air tightness of the device. Manually open the pneumatic throttle valve 1221, close the feed sealing ball valve 125 and the discharge sealing ball valve 1233, and connect the eleventh air pipe 146 to the container containing pure water. Click the "Air Tightness Check" button on the PC 31, use a small-volume syringe connected to the second air pipe 1222, inject air evenly, and observe the rate at which bubbles are generated by the pure water in the container. If the bubbles are generated smoothly and orderly, it means that the air tightness of the device is good.

[0128] (3): Vacuum the biogas production detection module 1. Manually close the pneumatic throttle valve 1221, use a small vacuum pump connected to the eleven air pipes 146, set the preset time period for vacuum extraction on the PC 31 and click the "Vacuum Extraction" button, and the device can automatically extract the air in the biogas production detection module 1 within the preset time period to form a vacuum environment.

[0129] (4): Add the mixed liquid. Insert the second liquid inlet pipe 126 into the configured mixed liquid container, manually open the feed sealing ball valve 125, click the "automatic liquid addition" button on the PC 31, and the device starts to automatically add the mixed liquid to the reaction tank 121 until the reaction tank 121 is basically filled. Finally, the remaining liquid can be injected into the reaction tank 121 using a syringe, etc., and the feed sealing ball valve 125 is closed. At this point, the feeding is completed.

[0130] Specifically, this step is vacuum feeding, and the specific implementation method is: manually close the pneumatic throttle valve 1221, the feed sealing ball valve 125, and the discharge sealing ball valve 1233, use a small vacuum pump to connect to the eleven air ducts 146, set the preset time period for vacuum extraction on the PC 31 and click the "Vacuum Extraction" button, the device starts to automatically extract the air in the biological gas production detection module 1 to form a vacuum environment.

[0131] In particular, when the device is in the process of extracting a vacuum, the single-chip microcomputer data acquisition processing unit 15 controls and issues instructions to close the first direct solenoid valve 1212, the second direct solenoid valve 1213, and the third direct solenoid valve 1224, and opens the "0, 2" two-port channels of the three-way solenoid valve 1228 and the exhaust solenoid valve 145. At this time, the stepper motor 1215 and the high-precision pressure transmitter 143 are also in a stopped state.

[0132] Furthermore, in order to improve the sealing and operability in the reaction tank 121, an automatic liquid feeding function is set, and the specific implementation method is: insert the second liquid inlet pipe 126 into the mixed liquid container, and open the feed sealing ball valve 125, and close the pneumatic throttle valve 1221 and the discharge sealing ball valve 1233; after the feeding is completed, close the feed sealing ball valve 125.

[0133] In particular, when the device is in the process of automatic feeding, the single-chip data acquisition processing unit 15 controls and issues instructions to close the first direct electromagnetic valve 1212, the second direct electromagnetic valve 1213, and the third direct electromagnetic valve 1224, and close the "0, 1, 2" port channels of the three-way electromagnetic valve 1228 and the exhaust electromagnetic valve 145. At this time, the stepper motor 1215 and the high-precision pressure transmitter 143 are also in a stopped state. In particular, the first liquid inlet pipe 124 and the second liquid inlet pipe 126 use sealing hoses with appropriately large inner diameters so that the oxygen-ammonia oxidation granular sludge can smoothly enter the reaction tank 121.

[0134] Therefore, a vacuum feeding design is adopted to strictly keep the entire system closed, and the total volume of biogas production can be kept constant, ensuring the accuracy of the activity index.

[0135] (5): Aeration of the biological gas production detection module 1. Connect an inert gas cylinder such as nitrogen or argon through the second air duct 1222, and manually adjust the pneumatic throttle valve 1221 to control the aeration flow rate. Set a preset aeration time period on the PC 31. For example, the continuous aeration time is generally 10 to 15 minutes, and there is no limit on this. Click the "aeration" button on the PC 31 to continue aeration during the aeration time period, exhaust the air in the device, and form an oxygen-free environment. After aeration is completed, close the pneumatic throttle valve 1221.

[0136] Specifically, after the mixed liquid is added into the reaction tank 121, the aeration operation is started, the inert gas cylinder such as nitrogen or argon is connected through the second air duct 1222, and the pneumatic throttle valve 1221 is manually adjusted to control the aeration flow rate. At this time, the feed sealing ball valve 125 and the discharge sealing ball valve 1233 are closed.

[0137] When the device is in the aeration process, the single-chip microcomputer data acquisition processing unit 15 controls and issues instructions to open the "0, 1" two-port channels of the three-way solenoid valve 1228, the third direct solenoid valve 1224, and the exhaust solenoid valve 145, while the first direct solenoid valve 1212, the second direct solenoid valve 1213, the stepper motor 1215, and the high-precision pressure transmitter 143 are still in a stopped state.

[0138] In particular, when the two-port channels "0, 1" of the three-way solenoid valve 1228 are opened, the two-port channels "0, 2" are immediately closed, and at this time, a part of the aeration gas enters the reaction tank 121 from the first air pipe 1220, and flows out to the atmosphere from the sixth air pipe 1229; at the same time, the third direct solenoid valve 1224 is opened, and another part of the aeration gas enters the U-shaped tube 131 through the third air pipe 1223 (AB section) and the fourth air pipe 1225 (CD section), and enters the gas collecting bottle 141 through the ninth air pipe 136, and finally flows out to the atmosphere through the tenth air pipe 144 and the eleventh air pipe 146. Therefore, through the above two-way parallel and separate aeration design, during the aeration process of the reaction tank 121, it is prevented that the water vapor is carried away by a large amount of aeration gas, causing the water vapor to enter the U-shaped tube 131, thereby improving the service life of the desiccant 134 and the indicator 135 in the U-shaped tube 131.

[0139] (6): Device operation. Click the "Start" button on the PC 31, and the biogas production detection module 1, the laser induced fluorescence detection module 2, and the data acquisition and processing module 3 start to run. The single-chip data acquisition and processing unit 15 collects and processes the pressure change collected by the high-precision pressure transmitter 143 and transmits it to the PC 31, and the laser induced fluorescence detection control unit 23 collects and processes the protein-like fluorescence intensity data detected in the liquid pool 214 and transmits it to the PC 31. The PC 31 uses a calculation formula to automatically couple the two parameters and comprehensively judge the activity index of the anaerobic ammonia oxidation sludge.

[0140] Specifically, when the biological gas production detection module 1 operates normally, the single-chip computer data acquisition processing unit 15 controls and issues instructions to close the first direct solenoid valve 1212, the second direct solenoid valve 1213, the third direct solenoid valve 1224, and the exhaust solenoid valve 145, open the two-port channels "0, 2" of the three-way solenoid valve 1228, and turn on the high-precision pressure transmitter 143 and the stepper motor 1215 in turn, so as to perform fermentation gas production and pressure measurement. The single-chip computer data acquisition processing unit 15 transmits the processed and calculated air pressure value to the PC 31 in real time. The calculation process of the single-chip computer data acquisition processing unit 15 is as follows:

[0141] When the high-precision pressure transmitter 143 reaches the preset pressure value, the exhaust solenoid valve 145 opens quickly and briefly, and the device is started to release pressure. The short time period for the exhaust solenoid valve 145 to be opened can be set, and the time period can be set to tens of milliseconds, which is not limited. When the set time period is reached, the exhaust solenoid valve 145 is quickly closed. At this time, the high-precision pressure transmitter 143 senses the upper air pressure value in the gas collection bottle 141, and the single-chip data acquisition processing unit 15 calculates the air pressure values ​​before and after the exhaust solenoid valve 145, records the drop value, and adds the drop value to the high-precision pressure transmitter 143 reading of the next cycle. The cycle is repeated in sequence to obtain a continuous curve of biological gas production pressure and time.

[0142] Therefore, by setting a preset pressure value for pressure relief, on the one hand, it can prevent the biogas production pressure from exceeding the pressure resistance value that the reaction device can withstand, causing gas leakage or even explosion of the reaction tank 121, gas collecting bottle 141, etc.; on the other hand, it can reduce the selected range of the high-precision pressure transmitter 143, thereby improving the sensitivity and accuracy of the measurement.

[0143] Furthermore, a temperature sensor 112 is arranged in the constant temperature water bath unit 11, and an atmospheric pressure sensor 16 is arranged around the device. The single-chip computer data acquisition and processing unit 15 collects the ambient temperature and atmospheric pressure values ​​at the same time during the process of collecting the biological gas production pressure data. Through real-time temperature and pressure compensation, the measured volume of biogas can be converted into a value under standard conditions (i.e., 0°C, 1 standard atmospheric pressure), thereby minimizing the measurement interference caused by changes in external environmental conditions.

[0144] It should be noted that the present application is tested in a closed circulation device. In this device, the amount of sampled anaerobic ammonium oxidation granular sludge, simulated wastewater and nutrient solution can be kept constant, and the N2 gas production can be easily obtained under a fixed total volume of biological gas distribution; the design of the circulating liquid outlet can detect the protein-like concentration after metabolism of the anaerobic ammonium oxidation granular sludge in real time online without affecting the reaction ecosystem; the N2 gas production and protein-like concentration do not interfere with each other, and can be used as corrections to the detection data.

[0145] (7): After the test is completed, the material is discharged. Click the "Finish" button on the PC 31, manually open the discharge sealing ball valve 1233, and empty the liquid in the reaction tank 121.

[0146] In one example, the embodiment of the present application also provides a detection method based on an anaerobic ammonium oxidation sludge detection system, the detection system includes a biogas production detection module, a laser induced fluorescence detection module, and a data acquisition and processing module; applied to the data acquisition and processing module, the method includes:

[0147] During the process of fermentation and gas production of the mixed liquid by the biological gas production detection module, the gas pressure value of the fermented gas is obtained; the laser induced fluorescence detection module performs laser fluorescence detection on the fermented mixed liquid to obtain fluorescence intensity data; based on the gas pressure value and the fluorescence intensity data, the activity index of the anaerobic ammonia oxidation sludge in the mixed liquid is determined; wherein the detection system is the system as indicated in the above embodiment.

[0148] Exemplarily, the detection system is a system as indicated in the above embodiments, and the structures and functions of the biological gas production detection module, the laser induced fluorescence detection module, and the data acquisition and processing module are not repeated here. The biological gas production detection module is used to perform operations such as fermentation, gas production, and gas drying on the mixed liquid. During this process, the data acquisition and processing module can collect the air pressure value of the fermented gas. During the fermentation process, the laser induced fluorescence detection module performs laser fluorescence detection on the fermented mixed liquid to obtain fluorescence intensity data. Finally, the data acquisition and processing module performs a comprehensive calculation of the air pressure value and the fluorescence intensity data to determine the activity index of the anaerobic ammonia oxidation sludge in the mixed liquid.

[0149] Those skilled in the art will readily appreciate other embodiments of the present disclosure after considering the specification and practicing the invention disclosed herein. This application is intended to cover any variations, uses or adaptations of the present disclosure that follow the general principles of the present disclosure and include common knowledge or customary techniques in the art that are not disclosed in the present disclosure. The specification and examples are intended to be exemplary only, and the true scope and spirit of the present disclosure are indicated by the following claims.

[0150] It should be understood that the present disclosure is not limited to the exact structures that have been described above and shown in the drawings, and that various modifications and changes may be made without departing from the scope thereof. The scope of the present disclosure is limited only by the appended claims.

Claims

1. A detection system based on anaerobic ammonium oxidation sludge, characterized in that: The detection system includes a biogas production detection module, a laser-induced fluorescence detection module, and a data acquisition and processing module; The biogas production detection module includes a constant temperature water bath unit, a fermentation reaction unit, a gas drying unit, a gas production metering unit and a single chip computer data acquisition and processing unit; the fermentation reaction unit has a feed inlet and a liquid outlet; The fermentation reaction unit, the gas drying unit and the gas production metering unit are located in the constant temperature water bath unit; the fermentation reaction unit and the gas drying unit are connected via an air duct, the gas drying unit and the gas production metering unit are connected via an air duct, the single-chip computer data acquisition and processing unit are respectively connected in circuit with the fermentation reaction unit, the gas drying unit and the gas production metering unit; the laser induced fluorescence detection module is connected in circuit with the fermentation reaction unit via the liquid outlet, and the data acquisition and processing module is respectively connected in circuit with the single-chip computer data acquisition and processing unit and the laser induced fluorescence detection module; The single-chip computer data acquisition and processing unit is used to control the fermentation reaction unit to ferment the initial mixed liquid entering through the feed inlet to generate fermented gas, and transport the gas to the gas drying unit; control the gas drying unit to dry the gas and transport the dried gas to the gas production metering unit; control the gas production metering unit to determine the gas pressure value of the gas; The laser induced fluorescence detection module is used to obtain the fermented mixed liquid through the liquid outlet, perform laser fluorescence detection on the fermented mixed liquid, and obtain fluorescence intensity data; wherein the fluorescence intensity data represents the growth information of the anaerobic ammonia-oxidizing bacteria in the mixed liquid during the fermentation process; The data acquisition and processing module is used to obtain the air pressure value sent by the single-chip data acquisition and processing unit and the fluorescence intensity data sent by the laser induced fluorescence detection module; and determine the activity index of the anaerobic ammonia oxidation sludge in the mixed liquid according to the air pressure value and the fluorescence intensity data.

2. The detection system according to claim 1, characterized in that: Determining the activity index of the anaerobic ammonium oxidation sludge in the mixed liquid according to the air pressure value and the fluorescence intensity data includes: Determine the linear fitting slope value of the gas production during the fermentation process and the linear fitting slope value of the protein-like concentration in the fermented mixed liquid according to the gas pressure value and the fluorescence intensity data; Determine the linear fitting slope value of the activity of the anaerobic ammonium oxidation sludge according to a preset slope formula, the linear fitting slope value of the gas production, and the linear fitting slope value of the protein-like concentration; Determining a target activity level corresponding to the linear fitting slope value of the activity of the anaerobic ammonium oxidation sludge according to a preset mapping relationship between the linear fitting slope value and the activity level; Determine the linear fitting slope value of the activity of the anaerobic ammonium oxidation sludge, the target activity level, and the gas production, which are all activity indicators of the anaerobic ammonium oxidation sludge in the mixed liquid; The slope formula is as follows: When K Nx ≥K N , M=K Nx / K N *R; when K Nx ≤K N , M=-K N / K Nx *R; Wherein, M represents the linear fitting slope value of the activity of the anaerobic ammonium oxidation sludge and the activity index of the anaerobic ammonium oxidation sludge; R is a preset correction coefficient; K N is the baseline linear slope of gas production in anammox sludge, K Nx is the linear slope of the gas production in the anaerobic ammonium-oxidizing granular sludge to be measured; The preset correction coefficient R is the linear fitting slope ratio between the protein concentration and the gas production of the anaerobic ammonium oxidation sludge during the fermentation process. The calculation formula of the correction coefficient R is as follows: When K Nx ≥K N When R = 1-|(K Qx / K Q -K Nx / K N ) / (K Qx / K Q )|; When K Nx ≤KN, R=1-|(K Q / K Qx -K N / K Nx ) / ( / K Q / K Qx )|;K Q Indicates the reference value of fluorescence intensity detection, K Qx Represents the fluorescence intensity of the protein-like substance to be detected.

3. The detection system according to claim 1, characterized in that: The constant temperature water bath unit comprises a constant temperature water bath and a temperature sensor located in the constant temperature water bath; The fermentation reaction unit includes a reaction tank, a first sealing cover arranged on the upper part of the reaction tank, a stepping motor, a coupling, a stirring rod, a stirring blade; a feed sealing ball valve, a pneumatic throttle valve, a three-way solenoid valve, a first straight solenoid valve, a second straight solenoid valve, a third straight solenoid valve, a discharge sealing ball valve, and a coarse filter membrane; A feed inlet is provided at the upper part of one side of the reaction tank, and the feed inlet is connected to the liquid outlet of the feed sealing ball valve through a first liquid inlet pipe; the liquid inlet of the feed sealing ball valve is communicated with the configured mixed liquid container through a second liquid inlet pipe; A circulating liquid outlet and a circulating liquid inlet are provided on one side of the reaction tank, and the positions of the circulating liquid outlet and the circulating liquid inlet are both lower than the feed inlet; coarse filter membranes are provided at the outlets of the circulating liquid outlet and the circulating liquid inlet, and the circulating liquid outlet is connected to the first straight electromagnetic valve through the first circulating liquid pipe, and the circulating liquid inlet is connected to the second straight electromagnetic valve through the second circulating liquid pipe; A discharge port is provided at a position close to the bottom of the reaction tank and away from the circulating liquid outlet, and the discharge port is connected to a discharge sealing ball valve through an eighth air guide pipe.

4. The detection system according to claim 3, characterized in that: An aeration air inlet is provided on one side of the upper portion of the first sealing cover, and the aeration air inlet is connected to the air outlet of the pneumatic throttle valve through a first air guide pipe; the air inlet of the pneumatic throttle valve is connected to the inert gas cylinder through a second air guide pipe; An air outlet is provided on the other side of the upper part of the first sealing cover, and the air outlet is connected to the air inlet of the three-way solenoid valve through the fifth air duct. The first air outlet of the three-way solenoid valve is connected to the atmospheric environment through the sixth air duct, and the second air outlet of the three-way solenoid valve is connected to the gas drying unit through the seventh air duct.

5. The detection system according to claim 4, characterized in that: The first air duct is connected to a three-way joint, the first interface of the three-way joint is connected to the second interface of the third direct solenoid valve through the third air duct, and the third interface of the third direct solenoid valve is connected to the fourth interface of the gas drying unit through the fourth air duct.

6. The detection system according to claim 1, characterized in that: The gas drying unit includes a U-shaped tube, a sealing plug located at the upper part of the U-shaped tube, a layered sheet, and a gas drying area and an indication area obtained by dividing the layered sheet; one side of the gas drying unit is connected to the gas production metering unit through a ninth gas guide pipe; The gas production metering unit includes a gas collecting bottle and a second sealing cover arranged on the upper part of the gas collecting bottle, and a high-precision pressure transmitter is installed on the upper part of the second sealing cover; a gas outlet on one side of the gas collecting bottle is connected to the gas inlet of the exhaust solenoid valve through the tenth air duct, and the gas outlet of the exhaust solenoid valve is connected to the atmospheric environment through the eleventh air duct; the exhaust solenoid valve is provided with a preset pressure value for exhaust.

7. The detection system according to claim 6, characterized in that: The data acquisition and processing module includes a personal computer; The single chip data acquisition processing unit is used to obtain the air pressure value of the gas through the high-precision pressure transmitter and send the air pressure value to the personal computer; The personal computer is used to obtain the air pressure value and the fluorescence intensity data sent by the laser induced fluorescence detection module.

8. The detection system according to any one of claims 1 to 7, characterized in that: The laser induced fluorescence detection module includes a circulating liquid outlet unit, a laser induced fluorescence detection unit, and a laser induced fluorescence detection control unit; The circulating liquid outlet unit and the laser induced fluorescence detection unit are separated by a preset light projection distance, and the laser induced fluorescence detection unit is circuit-connected to the laser induced fluorescence detection control unit; The laser induced fluorescence detection control unit is used to control the circulating liquid outlet unit to circulate and obtain the fermented mixed liquid; control the laser induced fluorescence detection unit to perform laser fluorescence detection on the fermented mixed liquid in the circulating liquid outlet unit to obtain fluorescence intensity data.

9. The detection system according to claim 8, characterized in that: The circulating liquid outlet unit includes a precision peristaltic pump, a one-way valve, a fine filter with a preset concentration value, and a liquid pool; The liquid inlet of the precision peristaltic pump is connected to the outlet of the first straight electromagnetic valve through the third circulating liquid pipe; the liquid outlet of the precision peristaltic pump, the one-way valve, the fine filter, and the liquid pool are connected in series through the fourth circulating liquid pipe, the fifth circulating liquid pipe, and the sixth circulating liquid pipe; the liquid pool is connected to the liquid inlet of the second straight electromagnetic valve through the seventh circulating liquid pipe; The laser induced fluorescence detection unit includes a laser light source, a laser filter, a spectroscope, a fluorescence filter, and a photodetector; The laser-induced fluorescence detection control unit comprises a microcontroller, and the microcontroller controls the precision peristaltic pump and the laser-induced fluorescence detection unit and performs data processing.

10. A detection method based on an anaerobic ammonium oxidation sludge detection system, characterized in that: The detection system includes a biogas production detection module, a laser-induced fluorescence detection module, and a data acquisition and processing module; Applied to the data acquisition and processing module, the method comprises: In the process of fermenting and gasifying the mixed liquid by the biological gas production detection module, obtaining the gas pressure value of the fermented gas; Performing laser-induced fluorescence detection on the mixed liquid in the fermentation process by the laser-induced fluorescence detection module to obtain fluorescence intensity data; Determining the activity index of the anaerobic ammonium oxidation sludge in the mixed liquid according to the air pressure value and the fluorescence intensity data; Wherein, the detection system is a system as described in any one of claims 1-9.

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