Fluorescence detection device

Abstract

The present invention relates to the field of molecular biology detection, and discloses a fluorescence detection apparatus, which comprises a reaction flat tube, a fluorescence exciter and a fluorescence detector, the fluorescence exciter comprises a first light emitting member, a second light emitting member, a third light emitting member, a fourth light emitting member and a beam combining assembly, the first light-emitting part, the second light-emitting part, the third light-emitting part and the fourth light-emitting part can respectively emit excitation light beams with different colors, and any one or more of the first light-emitting part, the second light-emitting part, the third light-emitting part and the fourth light-emitting part can independently emit excitation light beams with different colors; and the fluorescence detector can combine excitation light beams emitted by the first light-emitting part, the second light-emitting part, the third light-emitting part and the fourth light-emitting part respectively and emit the combined excitation light beams to the sample reagent along the light outlet, and is used for receiving and detecting the fluorescence light beams. A driving mechanism is not needed, the manufacturing cost of the fluorescence detection device is effectively reduced, the reliability of the fluorescence detection device is improved, and in addition, more-channel detection can be realized without adding an additional hardware structure.

Description

Technical Field

[0001] The invention belongs to the field of molecular biology detection, and in particular, relates to a fluorescence detection device. Background Art

[0002] The fluorescence detection device in the prior art is generally provided with a driving mechanism, and the driving mechanism drives the filter to rotate and switch, or the driving mechanism drives the optical element or sample to move and scan to achieve multi-channel detection. However, the setting of the driving mechanism not only increases the manufacturing cost of the fluorescence detection device, but also may bring hidden dangers to the reliability of the device during long-distance transportation and long-term use. Summary of the invention

[0003] In view of the above-mentioned deficiencies or defects in the prior art, the present invention provides a fluorescence detection device, aiming to solve the technical problems of high manufacturing cost and low reliability of the existing fluorescence detection devices.

[0004] To achieve the above object, the present invention provides a fluorescence detection device, comprising:

[0005] A flat reaction tube having a detection cavity for storing sample reagents;

[0006] A fluorescence exciter, comprising an excitation box body, a first light-emitting member, a second light-emitting member, a third light-emitting member, a fourth light-emitting member and a beam-combining component, wherein a first side wall of the excitation box body in a lateral direction is provided with a light outlet facing the side wall of the detection cavity, the first light-emitting member, the second light-emitting member, the third light-emitting member and the fourth light-emitting member are accommodated in the excitation box body and can respectively emit excitation light beams of different colors, and any one or more of the first light-emitting member, the second light-emitting member, the third light-emitting member and the fourth light-emitting member can individually emit excitation light beams of multiple different colors, the beam-combining component is accommodated in the excitation box body, can combine the excitation light beams respectively emitted by the first light-emitting member, the second light-emitting member, the third light-emitting member and the fourth light-emitting member and emit them along the light outlet to the sample reagent, so as to excite the sample reagent to emit a fluorescence light beam;

[0007] The fluorescence detector is used to receive and detect the fluorescence light beam.

[0008] Optionally, the first light-emitting element can independently emit blue and red excitation light beams, the second light-emitting element can independently emit purple and crimson excitation light beams, the third light-emitting element can independently emit a yellow excitation light beam, and the fourth light-emitting element can independently emit a green excitation light beam.

[0009] Optionally, the fluorescence exciter also includes a first excitation filter, a second excitation filter, a third excitation filter and a fourth excitation filter, the first excitation filter is arranged on the light-emitting side of the first light-emitting component and is set to a dual-bandpass filter with a central wavelength of 470nm and 630nm and a bandwidth of 20nm, the second excitation filter is arranged on the light-emitting side of the second light-emitting component and is set to a dual-bandpass filter with a central wavelength of 405nm and 682nm and a bandwidth of 20nm, the third excitation filter is arranged on the light-emitting side of the third light-emitting component and is set to a single bandpass filter with a central wavelength of 580nm and a bandwidth of 20nm, and the fourth excitation filter is arranged on the light-emitting side of the fourth light-emitting component and is set to a single bandpass filter with a central wavelength of 530nm and a bandwidth of 20nm.

[0010] Optionally, the first light-emitting member, the second light-emitting member, the third light-emitting member and the fourth light-emitting member are arranged in sequence along the longitudinal direction of the excitation box body, and the light-emitting sides thereof face the first side wall of the excitation box body.

[0011] Optionally, the fluorescence stimulator also includes a PCB board electrically connected to the first light-emitting element, the second light-emitting element, the third light-emitting element and the fourth light-emitting element respectively, the excitation box body has a second side wall opposite to the first side wall in the transverse direction, and the PCB board is attached to the outer side surface of the second side wall of the excitation box body and is detachably connected to the second side wall of the excitation box body.

[0012] Optionally, one of the outer side surfaces of the PCB board and the second side wall of the excitation box body is provided with a snap-fitting protrusion, and the other is provided with a snap-fitting groove, and the snap-fitting protrusion is snap-fitted with the snap-fitting groove.

[0013] Optionally, the light emitting side of the third light-emitting component is opposite to the light outlet; the beam combining component includes a first excitation dichroic mirror, a second excitation dichroic mirror, a third excitation dichroic mirror, a fourth excitation dichroic mirror and a fifth excitation dichroic mirror, the first excitation dichroic mirror, the second excitation dichroic mirror and the third excitation dichroic mirror are arranged side by side along the longitudinal direction of the excitation box body and are respectively located on the light paths of the first light-emitting component, the second light-emitting component and the third light-emitting component, the fourth excitation dichroic mirror and the fifth excitation dichroic mirror are arranged side by side along the longitudinal direction of the excitation box body and are respectively located on the light paths of the third light-emitting component and the fourth light-emitting component, and the third excitation dichroic mirror and the fourth excitation dichroic mirror are arranged at intervals on the light path of the third light-emitting component,

[0014] Among them, the first excitation dichroic mirror is used to reflect the first excitation light beam emitted by the first light-emitting component toward the third excitation dichroic mirror, the second excitation dichroic mirror is used to transmit the first excitation light beam and reflect the second excitation light beam emitted by the second light-emitting component toward the third excitation dichroic mirror, the fifth excitation dichroic mirror is used to reflect the fourth excitation light beam emitted by the fourth light-emitting component toward the fourth excitation dichroic mirror, the fourth excitation dichroic mirror is used to transmit the third excitation light beam emitted by the third light-emitting component and reflect the fourth excitation light beam toward the light exit, and the third excitation dichroic mirror is used to transmit the third excitation light beam and the fourth excitation light beam and reflect the first excitation light beam and the second excitation light beam toward the light exit.

[0015] Optionally, the excitation box body includes a detachably connected light-emitting component base and an exciter base, the light-emitting component base and the exciter base respectively include a light-emitting component accommodating cavity and an exciter accommodating cavity that are interconnected, the first light-emitting component, the second light-emitting component, the third light-emitting component and the fourth light-emitting component are accommodated in the light-emitting component accommodating cavity, the first excitation dichroic mirror, the second excitation dichroic mirror, the third excitation dichroic mirror, the fourth excitation dichroic mirror and the fifth excitation dichroic mirror are accommodated in the exciter accommodating cavity, and the light outlet is opened on the exciter base and is connected to the exciter accommodating cavity.

[0016] Optionally, the fluorescence detector includes a detection box body, a first detection component, a second detection component, a third detection component, a fourth detection component and a beam splitting assembly, the detection box body has a first side wall in the lateral direction with a light inlet facing the side wall of the detection cavity to receive the fluorescent light beam, the first detection component, the second detection component, the third detection component and the fourth detection component are accommodated in the detection box body, the beam splitting assembly is accommodated in the detection box body, and can split the fluorescent light beam and respectively direct it to the first detection component, the second detection component, the third detection component and the fourth detection component.

[0017] Optionally, the fluorescence detector also includes a first detection filter, a second detection filter, a third detection filter and a fourth detection filter, the first detection filter is arranged on the signal receiving side of the first detection element and is set to a dual-bandpass filter with a central wavelength of 520nm and 665nm and a bandwidth of 20nm, the second detection filter is arranged on the signal receiving side of the second detection element and is set to a dual-bandpass filter with a central wavelength of 470nm and 730nm and a bandwidth of 20nm and 40nm respectively, the third detection filter is arranged on the signal receiving side of the third detection element and is set to a single bandpass filter with a central wavelength of 610nm and a bandwidth of 20nm, and the fourth detection filter is arranged on the signal receiving side of the fourth detection element and is set to a single bandpass filter with a central wavelength of 565nm and a bandwidth of 20nm.

[0018] Optionally, the first detection member, the second detection member, the third detection member and the fourth detection member are arranged in sequence along the longitudinal direction of the detection box body and the signal receiving sides thereof are all facing the first side wall of the detection box body.

[0019] Optionally, the fluorescence detection device also includes a PD board electrically connected to the first detection member, the second detection member, the third detection member and the fourth detection member respectively, and the PD board is installed on the outside of the detection box body and the orientation of a side surface facing away from the detection box body is consistent with the orientation of the light-emitting side of the first light-emitting member, the second light-emitting member, the third light-emitting member and the fourth light-emitting member.

[0020] Optionally, the signal receiving side of the third detection element is opposite to the light inlet; the beam splitting assembly includes a first detection dichroic mirror, a second detection dichroic mirror, a third detection dichroic mirror, a fourth detection dichroic mirror and a fifth detection dichroic mirror, the first detection dichroic mirror, the second detection dichroic mirror and the third detection dichroic mirror are arranged side by side in the longitudinal direction of the detection box body and are respectively located on the signal receiving paths of the first detection element, the second detection element and the third detection element, the fourth detection dichroic mirror and the fifth detection dichroic mirror are arranged side by side in the longitudinal direction of the detection box body and are respectively located on the signal receiving paths of the third detection element and the fourth detection element, and the third detection dichroic mirror and the fourth detection dichroic mirror are arranged at intervals on the optical path of the third detection element,

[0021] Among them, the third detection dichroic mirror is used to reflect the first fluorescent beam and the second fluorescent beam corresponding to the first detection component and the second detection component respectively in the fluorescent beam incident along the light inlet to the first detection dichroic mirror and the second detection dichroic mirror respectively, and is also used to transmit the third fluorescent beam and the fourth fluorescent beam corresponding to the third detection component and the fourth detection component respectively in the fluorescent beam incident along the light inlet, the second detection dichroic mirror is used to reflect the second fluorescent beam to the second detection component and transmit the first fluorescent beam, the first detection dichroic mirror is used to reflect the first fluorescent beam to the first detection component, the fourth detection dichroic mirror is used to reflect the fourth fluorescent beam to the fifth detection dichroic mirror and transmit the third fluorescent beam, and the fifth detection dichroic mirror is used to reflect the fourth fluorescent beam to the fourth detection component.

[0022] Optionally, the direction of the light outlet of the excitation box body and the direction of the light inlet of the detection box body are arranged at an angle of 90°.

[0023] In the fluorescence detection device of the present invention, the first light-emitting component, the second light-emitting component, the third light-emitting component and the fourth light-emitting component can respectively emit excitation light beams of different colors, and the beam combining component can combine the excitation light beams respectively emitted by the first light-emitting component, the second light-emitting component, the third light-emitting component and the fourth light-emitting component and emit them along the light outlet to excite the sample reagent stored in the detection cavity of the reaction flat tube to emit a fluorescence light beam. In this way, at least four different fluorescence excitation channels can be formed, and corresponding fluorescence detectors can be configured to achieve four-channel detection without setting any driving mechanism, effectively reducing the manufacturing cost of the fluorescence detection device and improving the reliability of the fluorescence detection device; in addition, any one or more of the first light-emitting component, the second light-emitting component, the third light-emitting component and the fourth light-emitting component can independently emit excitation light beams of multiple different colors. In this way, the fluorescence excitation channels can be expanded to five, six or more than six. In this way, more channel detection can be achieved without adding additional hardware structure, that is, more channel detection can be achieved under the premise of ensuring that the fluorescence detection device is small in size and low in manufacturing cost.

[0024] Other features and advantages of the present invention will be described in detail in the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the following specific embodiments, they are used to explain the present invention but do not constitute a limitation of the present invention. In the accompanying drawings:

[0026] Figure 1 is a schematic structural diagram of a fluorescence detection device in one embodiment of the present invention;

[0027] Figure 2 for Figure 1 Schematic diagram of the internal structure of the fluorescence exciter;

[0028] Figure 3 for Figure 1 Schematic diagram of the external structure of the fluorescence exciter;

[0029] Figure 4 for Figure 1 Schematic diagram of the internal structure of the fluorescence detector;

[0030] Figure 5 for Figure 1 Schematic diagram of the external structure of the fluorescence detector.

[0031] Description of reference numerals:

[0032] 1 Reaction flat tube

[0033] 11. Detection chamber

[0034] 2 Fluorescence excitation

[0035] 21 Excitation box 21a Light-emitting element base

[0036] 21b Activator base 21c Activator cover

[0037] 22 first light emitting element 23 second light emitting element

[0038] 24 third light emitting element 25 fourth light emitting element

[0039] 26 First excitation dichroic mirror 27 Second excitation dichroic mirror

[0040] 28 Third excitation dichroic mirror 29 Fourth excitation dichroic mirror

[0041] 210 fifth excitation dichroic mirror 211 PCB board

[0042] 212 First excitation filter 213 Second excitation filter

[0043] 214 third excitation filter 215 fourth excitation filter

[0044] 3 Fluorescence detector

[0045] 31 Detection box body 31a Detection base

[0046] 31b Detection cover 32 First detection piece

[0047] 33 Second detection part 34 Third detection part

[0048] 35 Fourth detection element 36 First detection dichroic mirror

[0049] 37 Second detection dichroic mirror 38 Third detection dichroic mirror

[0050] 39 Fourth detection dichroic mirror 310 Fifth detection dichroic mirror

[0051] 311 PD board 312 first detection filter

[0052] 313 second detection filter 314 third detection filter

[0053] 315 Fourth detection filter DETAILED DESCRIPTION

[0054] The specific implementation of the present invention is described in detail below in conjunction with the accompanying drawings. It should be understood that the specific implementation described here is only used to illustrate and explain the present invention, and is not used to limit the present invention.

[0055] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features of the embodiments may be combined with each other.

[0056] In the present invention, unless otherwise specified, directional words such as "up, down, top, bottom" are usually used with reference to the directions shown in the drawings or are used to describe the relative positional relationships of components in the vertical, perpendicular or gravity direction.

[0057] The present invention will be described in detail below with reference to the accompanying drawings and in conjunction with embodiments.

[0058] The present invention first provides a fluorescence detection device.

[0059] In one embodiment, referring to Figure 1 To Attachment Figure 5As shown, the fluorescence detection device includes a reaction flat tube 1, a fluorescence exciter 2 and a fluorescence detector 3. The reaction flat tube 1 has a detection cavity 11 for storing sample reagents. The fluorescence exciter 2 includes an excitation box body 21, a first light-emitting member 22, a second light-emitting member 23, a third light-emitting member 24, a fourth light-emitting member 25 and a beam combining component. The excitation box body 21 has a first side wall in the horizontal direction with a light outlet facing the side wall of the detection cavity 11. The first light-emitting member 22, the second light-emitting member 23, the third light-emitting member 24 and the fourth light-emitting member 25 are accommodated in the excitation box body 21. And can respectively emit excitation light beams of different colors, and any one or more of the first light-emitting component 22, the second light-emitting component 23, the third light-emitting component 24 and the fourth light-emitting component 25 can individually emit excitation light beams of multiple different colors. The beam combining component is accommodated in the excitation box body 21, and can combine the excitation light beams respectively emitted by the first light-emitting component 22, the second light-emitting component 23, the third light-emitting component 24 and the fourth light-emitting component 25 and emit them along the light outlet to the sample reagent to excite the sample reagent to emit a fluorescent light beam. The fluorescence detector 3 is used to receive and detect the fluorescent light beam.

[0060] In this embodiment, the first light-emitting element 22, the second light-emitting element 23, the third light-emitting element 24 and the fourth light-emitting element 25 can respectively emit excitation light beams of different colors, and the beam combining component can combine the excitation light beams respectively emitted by the first light-emitting element 22, the second light-emitting element 23, the third light-emitting element 24 and the fourth light-emitting element 25 and emit them along the light outlet to excite the sample reagent stored in the detection cavity of the reaction flat tube 1 to emit a fluorescent light beam. In this way, at least four different fluorescence excitation channels can be formed, and the corresponding fluorescence detector 3 can be configured to achieve four-channel detection, and no driving mechanism is required, which effectively reduces the manufacturing cost of the fluorescence detection device and improves the reliability of the fluorescence detection device; in addition, any one or more of the first light-emitting element 22, the second light-emitting element 23, the third light-emitting element 24 and the fourth light-emitting element 25 can independently emit a plurality of different colors of excitation light beams. In this way, the fluorescence excitation channels can be expanded to five, six or more. In this way, more channel detection can be achieved without adding additional hardware structure, that is, more channel detection can be achieved under the premise of ensuring that the fluorescence detection device is small in size and low in manufacturing cost.

[0061] In this embodiment, the fluorescence exciter 2 may also include a focusing lens housed in the excitation box body 21; the focusing lens is blocked at the light outlet, and is used to focus the excitation light beam emitted along the light outlet into a linear light source, thereby improving the accuracy of the light beam projected to the sample reagent in the reaction flat tube. Specifically, the focusing lens can be a cylindrical mirror.

[0062] In addition, the fluorescence stimulator 2 may also include a plurality of excitation collimating lenses housed in the excitation box 21, and the plurality of excitation collimating lenses are respectively arranged on the light-emitting sides of the first light-emitting component 22, the second light-emitting component 23, the third light-emitting component 24 and the fourth light-emitting component 25, for collimating the light beams emitted by each light-emitting component.

[0063] In one embodiment, the first light-emitting element 22 can independently emit blue and red excitation beams, the second light-emitting element 23 can independently emit purple and crimson excitation beams, the third light-emitting element 24 can independently emit a yellow excitation beam, and the fourth light-emitting element 25 can independently emit a green excitation beam. In this way, six fluorescence excitation channels, namely FAM, CY5, Atto425, Quasar705, ROX, and HEX / VIC, can be formed.

[0064] Specifically, the first light-emitting element 22 may be a blue-red dual-color LED lamp, the second light-emitting element 23 may be a purple-dark-red dual-color LED lamp, the third light-emitting element 24 may be a yellow LED lamp, and the fourth light-emitting element 25 may be a green LED lamp.

[0065] In one embodiment, referring to Figure 2 As shown, the fluorescence exciter 2 also includes a first excitation filter 212, a second excitation filter 213, a third excitation filter 214 and a fourth excitation filter 215. The first excitation filter 212 is arranged on the light-emitting side of the first light-emitting element 22 and is set to be a dual-bandpass filter with a central wavelength of 470nm and 630nm and a bandwidth of 20nm. The second excitation filter 213 is arranged on the light-emitting side of the second light-emitting element 23 and is set to be a dual-bandpass filter with a central wavelength of 405nm and 682nm and a bandwidth of 20nm. The excitation filter 214 is arranged on the light-emitting side of the third light-emitting component 24 and is set to a single bandpass filter with a central wavelength of 580nm and a bandwidth of 20nm. The fourth excitation filter 215 is arranged on the light-emitting side of the fourth light-emitting component 25 and is set to a single bandpass filter with a central wavelength of 530nm and a bandwidth of 20nm. In this way, through the filtering effect of the filter, the excitation light beams emitted by each light-emitting component can accurately meet the specific wavelength requirements, effectively improve the accuracy and sensitivity of the excitation, and then improve the detection accuracy and sensitivity.

[0066] In actual application, the first excitation filter 212, the second excitation filter 213, the third excitation filter 214 and the fourth excitation filter 215 can be arranged to be opposite to the light emitting sides of the first light-emitting element 22, the second light-emitting element 23, the third light-emitting element 24 and the fourth light-emitting element 25, respectively, so as to be located on the parallel light paths of the first light-emitting element 22, the second light-emitting element 23, the third light-emitting element 24 and the fourth light-emitting element 25, respectively. Such an arrangement can optimize the filtering effect of each excitation filter.

[0067] In one embodiment, referring to Figure 2 As shown, the first light-emitting element 22, the second light-emitting element 23, the third light-emitting element 24 and the fourth light-emitting element 25 are arranged in sequence along the longitudinal direction of the excitation box body 21 and the light-emitting sides are all facing the first side wall of the excitation box body 21. Such an arrangement facilitates the wiring between the light-emitting elements and the control circuit board and simplifies the circuit wiring structure.

[0068] In one embodiment, referring to Figure 3 As shown, the fluorescent stimulator 2 also includes a PCB board 211 electrically connected to the first light-emitting component 22, the second light-emitting component 23, the third light-emitting component 24 and the fourth light-emitting component 25 respectively. The excitation box body 21 has a second side wall opposite to the first side wall in the horizontal direction. The PCB board 211 is attached to the outer side surface of the second side wall of the excitation box body 21 and is detachably connected to the second side wall of the excitation box body 21. Such an arrangement facilitates the disassembly and assembly of the PCB board 211.

[0069] In one embodiment, one of the outer side surfaces of the PCB board 211 and the second side wall of the excitation box body 21 is provided with a snap-fitting protrusion, and the other is provided with a snap-fitting groove, and the snap-fitting protrusion is snap-fitted with the snap-fitting groove.

[0070] It can be understood that the PCB board 211 can be detachably connected to the second side wall of the excitation box body 21 by snapping together the snapping protrusion and the snapping groove, and the snapping together can realize quick disassembly and assembly, further improving the convenience of disassembly and assembly of the PCB board.

[0071] In one embodiment, referring to Figure 2 As shown, the light emitting side of the third light emitting member 24 is opposite to the light outlet; the beam combining assembly includes a first excitation dichroic mirror 26, a second excitation dichroic mirror 27, a third excitation dichroic mirror 28, a fourth excitation dichroic mirror 29 and a fifth excitation dichroic mirror 210, the first excitation dichroic mirror 26, the second excitation dichroic mirror 27 and the third excitation dichroic mirror 28 are arranged side by side in the longitudinal direction of the excitation box body 21 and are respectively located on the light paths of the first light emitting member 22, the second light emitting member 23 and the third light emitting member 24, the fourth excitation dichroic mirror 29 and the fifth excitation dichroic mirror 210 are arranged side by side in the longitudinal direction of the excitation box body 21 and are respectively located on the light paths of the third light emitting member 24 and the fourth light emitting member 25, the third excitation dichroic mirror 28 and the fourth excitation dichroic mirror 29 are arranged at intervals on the light path of the third light emitting member 24,

[0072] Among them, the first excitation dichroic mirror 26 is used to reflect the first excitation light beam emitted by the first light-emitting component 22 to the third excitation dichroic mirror 28, the second excitation dichroic mirror 27 is used to transmit the first excitation light beam and reflect the second excitation light beam emitted by the second light-emitting component 23 to the third excitation dichroic mirror 28, the fifth excitation dichroic mirror 210 is used to reflect the fourth excitation light beam emitted by the fourth light-emitting component 25 to the fourth excitation dichroic mirror 29, the fourth excitation dichroic mirror 29 is used to transmit the third excitation light beam emitted by the third light-emitting component 24 and reflect the fourth excitation light beam to the light outlet, and the third excitation dichroic mirror 28 is used to transmit the third excitation light beam and the fourth excitation light beam and reflect the first excitation light beam and the second excitation light beam to the light outlet.

[0073] Through the reflection and / or transmission effect of the above-mentioned first excitation dichroic mirror 26, the second excitation dichroic mirror 27, the third excitation dichroic mirror 28, the fourth excitation dichroic mirror 29 and the fifth excitation dichroic mirror 210, the excitation light beams emitted by the first light-emitting component 22, the second light-emitting component 23, the third light-emitting component 24 and the fourth light-emitting component 25 are respectively combined and emitted along the light output port.

[0074] In practical applications, both the first excitation dichroic mirror 26 and the fifth excitation dichroic mirror 210 can be replaced by reflection mirrors.

[0075] In one embodiment, the excitation box body 21 includes a detachably connected light-emitting component base 21a and an exciter base, the light-emitting component base 21a and the exciter base respectively include a light-emitting component accommodating cavity and an exciter accommodating cavity that are interconnected, the first light-emitting component 22, the second light-emitting component 23, the third light-emitting component 24 and the fourth light-emitting component 25 are accommodated in the light-emitting component accommodating cavity, the first excitation dichroic mirror 26, the second excitation dichroic mirror 27, the third excitation dichroic mirror 28, the fourth excitation dichroic mirror 29 and the fifth excitation dichroic mirror 210 are accommodated in the exciter accommodating cavity, the light outlet is opened on the exciter base and is connected to the exciter accommodating cavity, such an arrangement realizes the modular installation of the excitation box body 21, and effectively improves the convenience of disassembly and assembly of the excitation box body 21.

[0076] Further, see Attachment Figure 3 As shown, the exciter base may also include an exciter base 21b and an exciter cover 21c. The exciter cover 21c can be detachably covered on the exciter base 21b to enclose the exciter base 21b to form an exciter accommodating cavity. The first excitation dichroic mirror 26, the second excitation dichroic mirror 27, the third excitation dichroic mirror 28, the fourth excitation dichroic mirror 29 and the fifth excitation dichroic mirror 210 are inserted into the exciter base 21b. Such an arrangement effectively improves the convenience of disassembly and assembly of the first excitation dichroic mirror 26, the second excitation dichroic mirror 27, the third excitation dichroic mirror 28, the fourth excitation dichroic mirror 29 and the fifth excitation dichroic mirror 210.

[0077] In one embodiment, referring to Figure 4 As shown, the fluorescence detector 3 includes a detection box body 31, a first detection member 32, a second detection member 33, a third detection member 34, a fourth detection member 35 and a beam splitting assembly. The detection box body 31 has a first side wall in the lateral direction with a light inlet facing the side wall of the detection cavity 11 to receive the fluorescent light beam. The first detection member 32, the second detection member 33, the third detection member 34 and the fourth detection member 35 are accommodated in the detection box body 31. The beam splitting assembly is accommodated in the detection box body 31, and can split the fluorescent light beam and respectively emit it to the first detection member 32, the second detection member 33, the third detection member 34 and the fourth detection member 35.

[0078] It can be understood that the first detection member 32, the second detection member 33, the third detection member 34, and the fourth detection member 35 are respectively configured corresponding to the first light-emitting member 22, the second light-emitting member 23, the third light-emitting member 24, and the fourth light-emitting member 25. That is, the fluorescent light beam excited by the excitation light beam emitted by the first light-emitting member 22 is received and detected by the first detection member 32, the fluorescent light beam excited by the excitation light beam emitted by the second light-emitting member 23 is received and detected by the second detection member 33, the fluorescent light beam excited by the excitation light beam emitted by the third light-emitting member 24 is received and detected by the third detection member 34, and the fluorescent light beam excited by the excitation light beam emitted by the fourth light-emitting member 25 is received and detected by the third detection member 34. The fluorescent light beam stimulated by the excitation light beam emitted by the optical element 25 is received and detected by the fourth detection element 35. For example, when the first light-emitting element 22, the second light-emitting element 23, the third light-emitting element 24 and the fourth light-emitting element 25 form six fluorescence excitation channels of FAM, CY5, Atto425, Quasar705, ROX, and HEX / VIC, the first detection element 32, the second detection element 33, the third detection element 34 and the fourth detection element 35 correspondingly form six fluorescence detection channels of FAM, CY5, Atto425, Quasar705, ROX, and HEX / VIC.

[0079] Specifically, the first detection element 32 , the second detection element 33 , the third detection element 34 , and the fourth detection element 35 may all be photoelectric detectors.

[0080] In one embodiment, referring to Figure 4As shown, the fluorescence detector 3 also includes a first detection filter 312, a second detection filter 313, a third detection filter 314 and a fourth detection filter 315. The first detection filter 312 is arranged on the signal receiving side of the first detection element 32 and is set to be a dual-bandpass filter with a central wavelength of 520nm and 665nm and a bandwidth of 20nm. The second detection filter 313 is arranged on the signal receiving side of the second detection element 33 and is set to be a dual-bandpass filter with a central wavelength of 470nm and 730nm and a bandwidth of 20nm and 40nm respectively. Dual-bandpass filter, the third detection filter 314 is arranged on the signal receiving side of the third detection element 34 and is set to a single bandpass filter with a central wavelength of 610nm and a bandwidth of 20nm, the fourth detection filter 315 is arranged on the signal receiving side of the fourth detection element 35 and is set to a single bandpass filter with a central wavelength of 565nm and a bandwidth of 20nm. In this way, through the filtering effect of the detection filter, the fluorescent light beam emitted to each detection element can accurately meet the specific wavelength requirements, effectively improving the accuracy and sensitivity of the detection.

[0081] In actual application, the first detection filter 312, the second detection filter 313, the third detection filter 314 and the fourth detection filter 315 can be arranged to be opposite to the signal receiving sides of the first detection element 32, the second detection element 33, the third detection element 34 and the fourth detection element 35 respectively. Such arrangement can optimize the filtering effect of each detection filter.

[0082] In one embodiment, referring to Figure 4 As shown, the first detection member 32, the second detection member 33, the third detection member 34 and the fourth detection member 35 are arranged in sequence along the longitudinal direction of the detection box body 31 and the signal receiving sides are all facing the first side wall of the detection box body 31. Such an arrangement facilitates the wiring between the detection members and the circuit board and simplifies the circuit wiring structure.

[0083] In one embodiment, referring to Figure 5 As shown, the fluorescence detection device also includes a PD board 311 electrically connected to the first detection member 32, the second detection member 33, the third detection member 34 and the fourth detection member 35 respectively. The PD board 311 is installed on the outside of the detection box body 31 and the direction of the side facing away from the detection box body 31 is consistent with the direction of the light-emitting side of the first light-emitting member 22, the second light-emitting member 23, the third light-emitting member 24 and the fourth light-emitting member 25. With this arrangement, the PD board 311 can be consistent with the installation direction of multiple light-emitting members, which is convenient for automated production line production and installation.

[0084] In one embodiment, referring to Figure 4As shown, the signal receiving side of the third detection member 34 is opposite to the light inlet; the beam splitting assembly includes a first detection dichroic mirror 36, a second detection dichroic mirror 37, a third detection dichroic mirror 38, a fourth detection dichroic mirror 39 and a fifth detection dichroic mirror 310, the first detection dichroic mirror 36, the second detection dichroic mirror 37 and the third detection dichroic mirror 38 are arranged side by side in the longitudinal direction of the detection box body 31 and are respectively located on the signal receiving paths of the first detection member 32, the second detection member 33 and the third detection member 34, the fourth detection dichroic mirror 39 and the fifth detection dichroic mirror 310 are arranged side by side in the longitudinal direction of the detection box body 31 and are respectively located on the signal receiving paths of the third detection member 34 and the fourth detection member 35, the third detection dichroic mirror 38 and the fourth detection dichroic mirror 39 are arranged at intervals on the optical path of the third detection member 34,

[0085] Among them, the third detection dichroic mirror 38 is used to reflect the first fluorescent light beam and the second fluorescent light beam corresponding to the first detection component 32 and the second detection component 33 respectively in the fluorescent light beam incident along the light inlet to the first detection dichroic mirror 36 and the second detection dichroic mirror 37 respectively, and is also used to transmit the third fluorescent light beam and the fourth fluorescent light beam corresponding to the third detection component 34 and the fourth detection component 35 respectively in the fluorescent light beam incident along the light inlet. The second detection dichroic mirror 37 is used to reflect the second fluorescent light beam toward the second detection component 33 and transmit the first fluorescent light beam. The first detection dichroic mirror 36 is used to reflect the first fluorescent light beam toward the first detection component 32. The fourth detection dichroic mirror 39 is used to reflect the fourth fluorescent light beam toward the fifth detection dichroic mirror 310 and transmit the third fluorescent light beam. The fifth detection dichroic mirror 310 is used to reflect the fourth fluorescent light beam toward the fourth detection component 35.

[0086] In this embodiment, the fluorescence detector 3 also includes a detection collimating lens, a plurality of focusing lenses and a plurality of colored glass filters housed in the detection box body 31. The detection collimating lens is blocked at the light inlet and is used to collimate the fluorescent light beam incident along the light inlet. Specifically, the collimating lens can be a cylindrical mirror; the first detection component 32, the second detection component 33, the third detection component 34 and the fourth detection component 35 are respectively provided with a colored glass filter and a focusing lens from near to far from their respective signal receiving sides.

[0087] In practical applications, both the first detection dichroic mirror 36 and the fifth detection dichroic mirror 310 can be replaced by reflective mirrors.

[0088] In one embodiment, referring to Figure 1As shown, the direction of the light outlet of the excitation box body 21 and the direction of the light inlet of the detection box body 31 are set at an angle of 90°. This setting effectively reduces the background interference of the excitation light beam and the fluorescent light beam and improves the sensitivity of fluorescence detection.

[0089] In one embodiment, referring to Figure 5 As shown, the detection box body 31 includes a detection base 31a and a detection cover 31b, and the detection cover 31b is detachably covered on the detection base 31a to enclose the detection base 31a to form a detection accommodating cavity, and the first detection member 32, the second detection member 33, the third detection member 34, the fourth detection member 35, the first detection dichroic mirror 36, the second detection dichroic mirror 37, the third detection dichroic mirror 38, the fourth detection dichroic mirror 39 and the fifth detection dichroic mirror 310 are all accommodated in the detection accommodating cavity and inserted on the detection base 31a. Such a configuration effectively improves the convenience of disassembly and assembly of the first detection member 32, the second detection member 33, the third detection member 34, the fourth detection member 35, the first detection dichroic mirror 36, the second detection dichroic mirror 37, the third detection dichroic mirror 38, the fourth detection dichroic mirror 39 and the fifth detection dichroic mirror 310.

[0090] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

[0091] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

[0092] In addition, various embodiments of the present invention may be arbitrarily combined, and as long as they do not violate the concept of the present invention, they should also be regarded as the contents disclosed by the present invention.

Claims

1. A fluorescence detection device, characterized in that: include: A flat reaction tube (1) having a detection cavity (11) for storing sample reagents; A fluorescence exciter (2) comprises an excitation box body (21), a first light-emitting element (22), a second light-emitting element (23), a third light-emitting element (24), a fourth light-emitting element (25), and a beam combining component, wherein the excitation box body (21) has a first side wall in a lateral direction with a light outlet facing the side wall of the detection cavity (11), the first light-emitting element (22), the second light-emitting element (23), the third light-emitting element (24), and the fourth light-emitting element (25) are accommodated in the excitation box body (21) and can respectively emit excitation light beams of different colors, and the Any one or more of the first light-emitting element (22), the second light-emitting element (23), the third light-emitting element (24) and the fourth light-emitting element (25) can independently emit excitation light beams of multiple different colors. The beam combining component is accommodated in the excitation box body (21), and can combine the excitation light beams respectively emitted by the first light-emitting element (22), the second light-emitting element (23), the third light-emitting element (24) and the fourth light-emitting element (25) and emit them along the light outlet to the sample reagent, so as to excite the sample reagent to emit a fluorescent light beam; The fluorescence detector (3) is used for receiving and detecting the fluorescence light beam.

2. The fluorescence detection device according to claim 1, characterized in that: The first light-emitting element (22) can independently emit blue and red excitation light beams, the second light-emitting element (23) can independently emit purple and deep red excitation light beams, the third light-emitting element (24) can independently emit a yellow excitation light beam, and the fourth light-emitting element (25) can independently emit a green excitation light beam.

3. The fluorescence detection device according to claim 2, characterized in that: The fluorescence exciter (2) further comprises a first excitation filter (212), a second excitation filter (213), a third excitation filter (214) and a fourth excitation filter (215); the first excitation filter (212) is arranged on the light-emitting side of the first light-emitting element (22) and is configured as a dual-bandpass filter with central wavelengths of 470 nm and 630 nm and a bandwidth of 20 nm; the second excitation filter (213) is arranged on the light-emitting side of the second light-emitting element (23) and is configured as a dual-bandpass filter with central wavelengths of 470 nm and 630 nm and a bandwidth of 20 nm. The third excitation filter (214) is arranged on the light-emitting side of the third light-emitting element (24) and is arranged as a dual-bandpass filter with central wavelengths of 405 nm and 682 nm and a bandwidth of 20 nm. The third excitation filter (214) is arranged on the light-emitting side of the third light-emitting element (24) and is arranged as a single-bandpass filter with a central wavelength of 580 nm and a bandwidth of 20 nm. The fourth excitation filter (215) is arranged on the light-emitting side of the fourth light-emitting element (25) and is arranged as a single-bandpass filter with a central wavelength of 530 nm and a bandwidth of 20 nm.

4. The fluorescence detection device according to claim 1, characterized in that: The first light-emitting component (22), the second light-emitting component (23), the third light-emitting component (24) and the fourth light-emitting component (25) are arranged in sequence along the longitudinal direction of the excitation box body (21) and their light-emitting sides face the first side wall of the excitation box body (21).

5. The fluorescence detection device according to claim 4, characterized in that: The fluorescent stimulator (2) further comprises a PCB board (211) electrically connected to the first light-emitting element (22), the second light-emitting element (23), the third light-emitting element (24) and the fourth light-emitting element (25), respectively; the stimulating box body (21) comprises a second side wall opposite to the first side wall in the transverse direction; the PCB board (211) is attached to the outer side surface of the second side wall of the stimulating box body (21) and is detachably connected to the second side wall of the stimulating box body (21).

6. The fluorescence detection device according to claim 5, characterized in that: One of the outer side surfaces of the PCB board (211) and the second side wall of the excitation box body (21) is provided with a snap-fitting protrusion, and the other is provided with a snap-fitting groove, and the snap-fitting protrusion is snap-fitted with the snap-fitting groove.

7. The fluorescence detection device according to claim 4, characterized in that: The light emitting side of the third light emitting element (24) faces the light outlet; the beam combining assembly comprises a first excitation dichroic mirror (26), a second excitation dichroic mirror (27), a third excitation dichroic mirror (28), a fourth excitation dichroic mirror (29) and a fifth excitation dichroic mirror (210), wherein the first excitation dichroic mirror (26), the second excitation dichroic mirror (27) and the third excitation dichroic mirror (28) are arranged side by side in the longitudinal direction of the excitation box body (21) and are respectively located at the first excitation dichroic mirror (26), the second excitation dichroic mirror (27) and the third excitation dichroic mirror (28) On the optical paths of the first light-emitting element (22), the second light-emitting element (23) and the third light-emitting element (24), the fourth excitation dichroic mirror (29) and the fifth excitation dichroic mirror (210) are arranged side by side in the longitudinal direction of the excitation box body (21) and are respectively located on the optical paths of the third light-emitting element (24) and the fourth light-emitting element (25), and the third excitation dichroic mirror (28) and the fourth excitation dichroic mirror (29) are arranged at intervals on the optical path of the third light-emitting element (24). The first excitation dichroic mirror (26) is used to reflect the first excitation light beam emitted by the first light-emitting component (22) toward the third excitation dichroic mirror (28); the second excitation dichroic mirror (27) is used to transmit the first excitation light beam and reflect the second excitation light beam emitted by the second light-emitting component (23) toward the third excitation dichroic mirror (28); the fifth excitation dichroic mirror (210) is used to reflect the fourth excitation light beam emitted by the fourth light-emitting component (25) toward the fourth excitation dichroic mirror (29); the fourth excitation dichroic mirror (29) is used to transmit the third excitation light beam emitted by the third light-emitting component (24) and reflect the fourth excitation light beam toward the light outlet; and the third excitation dichroic mirror (28) is used to transmit the third excitation light beam and the fourth excitation light beam and reflect the first excitation light beam and the second excitation light beam toward the light outlet.

8. The fluorescence detection device according to claim 7, characterized in that: The excitation box body (21) comprises a detachably connected light-emitting component base (21a) and an exciter base, wherein the light-emitting component base (21a) and the exciter base respectively comprise a light-emitting component accommodating cavity and an exciter accommodating cavity which are interconnected, wherein the first light-emitting component (22), the second light-emitting component (23), the third light-emitting component (24) and the fourth light-emitting component (25) are accommodated in the light-emitting component accommodating cavity, the first excitation dichroic mirror (26), the second excitation dichroic mirror (27), the third excitation dichroic mirror (28), the fourth excitation dichroic mirror (29) and the fifth excitation dichroic mirror (210) are accommodated in the exciter accommodating cavity, and the light outlet is opened on the exciter base and is connected to the exciter accommodating cavity.

9. The fluorescence detection device according to claim 4, characterized in that: The fluorescence detector (3) comprises a detection box body (31), a first detection component (32), a second detection component (33), a third detection component (34), a fourth detection component (35) and a beam splitting assembly. The detection box body (31) is provided with a light inlet facing the side wall of the detection cavity (11) on a first side wall in a lateral direction so as to receive the fluorescence light beam. The first detection component (32), the second detection component (33), the third detection component (34) and the fourth detection component (35) are accommodated in the detection box body (31). The beam splitting assembly is accommodated in the detection box body (31) and is capable of splitting the fluorescence light beam and emitting the fluorescence light beam to the first detection component (32), the second detection component (33), the third detection component (34) and the fourth detection component (35) respectively.

10. The fluorescence detection device according to claim 9, characterized in that: The fluorescence detector (3) further comprises a first detection filter (312), a second detection filter (313), a third detection filter (314) and a fourth detection filter (315); the first detection filter (312) is arranged on the signal receiving side of the first detection element (32) and is configured as a dual-bandpass filter having center wavelengths of 520 nm and 665 nm and a bandwidth of 20 nm; the second detection filter (313) is arranged on the signal receiving side of the second detection element (33) The third detection filter (314) is arranged on the signal receiving side of the third detection element (34) and is arranged as a single bandpass filter with a central wavelength of 610 nm and a bandwidth of 20 nm. The fourth detection filter (315) is arranged on the signal receiving side of the fourth detection element (35) and is arranged as a single bandpass filter with a central wavelength of 565 nm and a bandwidth of 20 nm.

11. The fluorescence detection device according to claim 9, characterized in that: The first detection member (32), the second detection member (33), the third detection member (34) and the fourth detection member (35) are arranged in sequence along the longitudinal direction of the detection box body (31) and the signal receiving sides thereof are all facing the first side wall of the detection box body (31).

12. The fluorescence detection device according to claim 11, characterized in that: The fluorescence detection device further comprises a PD board (311) electrically connected to the first detection member (32), the second detection member (33), the third detection member (34) and the fourth detection member (35) respectively; the PD board (311) is mounted on the outer side of the detection box body (31) and the orientation of a side facing away from the detection box body (31) is consistent with the orientation of the light-emitting side of the first light-emitting member (22), the second light-emitting member (23), the third light-emitting member (24) and the fourth light-emitting member (25).

13. The fluorescence detection device according to claim 11, characterized in that: The signal receiving side of the third detection element (34) faces the light inlet; the beam splitting assembly comprises a first detection dichroic mirror (36), a second detection dichroic mirror (37), a third detection dichroic mirror (38), a fourth detection dichroic mirror (39) and a fifth detection dichroic mirror (310); the first detection dichroic mirror (36), the second detection dichroic mirror (37) and the third detection dichroic mirror (38) are arranged side by side in the longitudinal direction of the detection box body (31) and are respectively located at the first detection The detection box body (31) is provided with a first detection element (32), a second detection element (33) and a signal receiving path of the third detection element (34); the fourth detection dichroic mirror (39) and the fifth detection dichroic mirror (310) are arranged side by side in the longitudinal direction of the detection box body (31) and are respectively located on the signal receiving paths of the third detection element (34) and the fourth detection element (35); the third detection dichroic mirror (38) and the fourth detection dichroic mirror (39) are arranged at intervals on the optical path of the third detection element (34); The third detection dichroic mirror (38) is used to reflect the first fluorescent light beam and the second fluorescent light beam corresponding to the first detection element (32) and the second detection element (33) respectively in the fluorescent light beam incident along the light inlet to the first detection dichroic mirror (36) and the second detection dichroic mirror (37), and is also used to transmit the third fluorescent light beam and the fourth fluorescent light beam corresponding to the third detection element (34) and the fourth detection element (35) respectively in the fluorescent light beam incident along the light inlet, the second detection dichroic mirror (37) is used to reflect the second fluorescent light beam to the second detection element (33) and transmit the first fluorescent light beam, the first detection dichroic mirror (36) is used to reflect the first fluorescent light beam to the first detection element (32), the fourth detection dichroic mirror (39) is used to reflect the fourth fluorescent light beam to the fifth detection dichroic mirror (310) and transmit the third fluorescent light beam, and the fifth detection dichroic mirror (310) is used to reflect the fourth fluorescent light beam to the fourth detection element (35).

14. The fluorescence detection device according to claim 9, characterized in that: The direction of the light outlet of the excitation box body (21) and the direction of the light inlet of the detection box body (31) are arranged at an angle of 90°.

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