Analysis method and device for migration life history of coilia mystus

Abstract

The invention discloses a method and device for analyzing the migration life history of coilia ectenes, and relates to the field of fish ecology. The otolith is subjected to embedding, grinding and polishing treatment, and the embedding, grinding and polishing treatment processes are improved, so that the efficiency and quality of the otolith grinding piece are improved; according to the method, the otolith mill is taken as a raw material, the element content of the otolith mill is measured through an analysis technology, an element distribution curve with smaller errors can be obtained more quickly and more accurately, then the migration life history of the coilia mystus is analyzed based on the element distribution curve, and a scientific basis is provided for breeding management of the coilia mystus and sustainable utilization of fishery resources. According to the method, the analysis efficiency is improved, errors are reduced, the coilia mystus migration life history research based on the otolith is more reliable, and important data are contributed to protection and development of fishery resources of coilia mystus.

Description

Technical Field

[0001] The invention relates to the field of fish ecology, and in particular to an analysis method and device for the migratory life history of Coilia chrysogena. Background Art

[0002] As an economic migratory fish, the anchovy has become an important fishery resource in the Yangtze River Estuary and the Pearl River Estuary due to its rich nutritional value and delicious taste. However, in recent years, due to factors such as overfishing, environmental pollution and habitat degradation, the resources of the anchovy have dropped sharply and there is almost no fishing season. The otoliths of fish record the characteristics of the water environment in which they live throughout their life cycle, and changes in the water environment lead to changes in the trace elements of the otoliths. By analyzing the relevant information of the surrounding water environment and the trace elements in the otoliths, it plays an important role in analyzing the life history of fish migration, reproduction, spawning, etc., as well as reconstructing the habitat such as temperature, salinity, and food.

[0003] At present, the research on the migratory life history of the anchovy based on otoliths includes particle induced X-ray fluorescence analysis, electron probe analysis and laser ablation inductively coupled plasma mass spectrometry analysis of the anchovy. However, the existing technology still has defects, such as the processing method for the anchovy is too complicated, the damage to the anchovy sample is great, and the different processing methods of the anchovy lead to poor analysis results. Therefore, a new research method for the migratory life history of the anchovy based on otoliths is urgently needed to solve the defects of the existing technology. Summary of the invention

[0004] The invention provides a method and device for analyzing the migratory life history of anchovies, so as to solve the technical problem that the prior art cannot efficiently and accurately analyze the migratory life history of anchovies based on otoliths.

[0005] In order to solve the above technical problems, an embodiment of the present invention provides a method for analyzing the migratory life history of Coilia striata, comprising:

[0006] Step S101: According to a preset otolith processing method, the initial otoliths are cleaned, embedded, ground and polished to obtain a smooth otolith grinding disc;

[0007] Step S102: Obtaining an element distribution curve according to a preset analysis technique in combination with a smooth otolith grind;

[0008] Step S103: Analyze the migratory life history of Coilia spp. according to the element distribution curve.

[0009] It can be understood that, compared with the prior art, the present invention embeds, grinds and polishes otoliths, and improves the efficiency and quality of otolith grinding discs by improving the embedding, grinding and polishing processes; and by analyzing the element content of the otolith grinding discs through analytical technology, it is possible to obtain element distribution curves with smaller errors more quickly and accurately, and then analyze the migratory life history of the silver anchovy based on the element distribution curve, making the research on the migratory life history of the silver anchovy based on otoliths more reliable, contributing important data to the protection and development of the fishery resources of the silver anchovy, and providing a scientific basis for the breeding management of the silver anchovy and the sustainable utilization of fishery resources.

[0010] As a preferred solution, the initial otoliths are cleaned, embedded, ground and polished according to a preset otolith processing method to obtain a smooth otolith grinding disc, which specifically includes:

[0011] collecting initial otoliths, and cleaning the initial otoliths to obtain cleaned otoliths;

[0012] The cleaned otoliths are embedded according to the preset curing material to obtain an otolith acrylic block;

[0013] According to the preset grinding tools and suspension, the otolith acrylic block is ground and polished in sequence to obtain a smooth otolith grinding disc.

[0014] This preferred solution effectively reduces the time required for the embedding process and reduces the embedding cost by selecting a specific solidifying material; at the same time, the use of preset grinding tools and suspensions significantly improves the quality of the grinding disc and the efficiency of grinding and polishing.

[0015] As a preferred solution, the method of embedding the cleaned otoliths according to the preset solidifying material to obtain the otolith acrylic block specifically includes:

[0016] Place the cleaned otoliths in a preset mold dripped with preset glue;

[0017] According to a preset weight ratio, the acrylic powder and the acrylic curing agent are mixed and stirred, and poured into a preset mold to embed the cleaned otoliths;

[0018] After the preset embedding time, an acrylic block of otoliths is obtained.

[0019] This preferred solution greatly shortens the time and cost required for embedding by using special acrylic for cold embedding, thereby improving the efficiency of obtaining otolith grinding slices.

[0020] As a preferred solution, the otolith acrylic block is ground and polished in sequence according to the preset grinding tool and suspension to obtain a smooth otolith grinding disc, which specifically includes:

[0021] Place the otolith acrylic block on the preset grinding tool and mark the core of the otolith in the otolith acrylic block;

[0022] Using the first water-abrasive sandpaper and the second water-abrasive sandpaper in sequence to grind the single side of the otolith acrylic block until the core of the otolith in the otolith acrylic block is exposed, thereby obtaining a rough otolith grind sheet;

[0023] The preset first alumina suspension and the second alumina suspension are obtained, and the rough otolith grinding disc is polished in combination with the gradient particle size polishing method to obtain a smooth otolith grinding disc.

[0024] This preferred solution uses only two different mesh sizes of water-abrasive sandpaper for single-sided grinding. Compared with the prior art that uses multiple mesh sizes of water-abrasive sandpaper for double-sided grinding, it greatly reduces the types of sandpaper meshes and grinding steps, and significantly improves the grinding efficiency. At the same time, the combination of two different particle sizes of aluminum oxide suspension and gradient particle size polishing method effectively reduces scratches on the surface of the grinding disc, thereby improving the overall quality of the grinding disc.

[0025] As a preferred solution, the element distribution curve is obtained according to the preset analysis technology in combination with a smooth otolith grinding disc, specifically including:

[0026] The smooth otolith grinding disc is cleaned and coated to obtain the otolith grinding disc for analysis;

[0027] Using a preset electron probe micro-area analyzer and combining preset analysis parameters, the scanning line of the otolith grinding piece used for analysis is scanned to obtain an element distribution curve.

[0028] This preferred solution uses an electron probe micro-analyzer and preset analysis parameters to effectively shorten the scanning time and reduce the cost, while ensuring the accuracy of the element distribution curve.

[0029] As a preferred solution, the preset analysis parameters specifically include:

[0030] The accelerating voltage is 15 kV and the electron beam current is 5 × 10 -8 A, beam spot diameter is 1 μm, dwell time at each point is 500 ms, and dot interval is 1 μm.

[0031] This preferred solution uses an accelerating voltage of 15 kV to ensure that the analyzer can have a higher detection limit and a lower standard deviation. -8 A's electron beam current can ensure that the excitation capacity is increased while the degree of otolith damage is low. The beam spot diameter and dot interval of 1μm can increase the number of dots and shorten the dot interval to ensure the continuity and accuracy of the data and avoid the loss of environmental information due to the large dot interval. The dot efficiency can be improved through the 500ms dwell time.

[0032] As a preferred solution, the migratory life history of Coilia striata is analyzed according to the element distribution curve, specifically including:

[0033] According to the preset drawing library, a line graph was constructed with the distance from the otolith core as the horizontal axis and the element content ratio as the vertical axis, and the migratory life history of the silver carp was analyzed based on the line graph.

[0034] This preferred solution combines the longest radius of otoliths with the element content ratio to construct a line graph through a drawing library, so that the migratory life history of the golden anchovy can be analyzed intuitively.

[0035] Accordingly, the embodiment of the present invention provides an analysis device for the migratory life history of Coilia striata, comprising: an otolith grinding slice acquisition module, an element distribution curve acquisition module and a life history analysis module;

[0036] The otolith grinding disc acquisition module is used to clean, embed, grind and polish the initial otoliths according to a preset otolith processing method to obtain a smooth otolith grinding disc;

[0037] The element distribution curve acquisition module is used to obtain the element distribution curve according to a preset analysis technique in combination with a smooth otolith grinding disc;

[0038] The life history analysis module is used to analyze the migratory life history of Coilia spheniscus according to the element distribution curve.

[0039] As a preferred solution, the otolith grinding piece acquisition module includes: an otolith grinding piece acquisition unit;

[0040] The otolith grinding piece obtaining unit is used to collect initial otoliths, clean the initial otoliths, and obtain cleaned otoliths;

[0041] The cleaned otoliths are embedded according to the preset curing material to obtain an otolith acrylic block;

[0042] According to the preset grinding tools and suspension, the otolith acrylic block is ground and polished in sequence to obtain a smooth otolith grinding disc.

[0043] As a preferred solution, the otolith grinding piece obtaining unit comprises: an otolith embedding subunit;

[0044] The otolith embedding subunit is used to place the cleaned otoliths in a preset mold dripped with preset glue;

[0045] According to a preset weight ratio, the acrylic powder and the acrylic curing agent are mixed and stirred, and poured into a preset mold to embed the cleaned otoliths;

[0046] After the preset embedding time, an acrylic block of otoliths is obtained.

[0047] It can be understood that the present device has made improvements in the embedding, grinding and polishing of otoliths compared with the existing technology, which has significantly improved the efficiency and quality of preparing otolith grinding discs; in addition, by improving the parameters of the machine and using the electron probe analysis technology to analyze the element content of the otolith grinding discs, it is possible to obtain the element distribution curve with smaller errors faster and more accurately. Based on the element distribution curve, the migratory life history of the silver anchovy can be deeply analyzed, and the migratory life history of the silver anchovy based on otoliths can be analyzed efficiently and accurately. This not only provides a scientific basis for the management of silver anchovy breeding and the sustainable utilization of fishery resources, but also further contributes important data to the protection and development of fishery resources of silver anchovy. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] Figure 1 : A flow chart of the steps of a method for analyzing the migratory life history of Coilia striata provided by an embodiment of the present invention;

[0049] Figure 2 : A schematic diagram of the overall appearance structure of an otolith provided by an embodiment of the present invention;

[0050] Figure 3 : A schematic diagram of the structure of fixing otoliths on a semi-automatic grinding and polishing machine provided by an embodiment of the present invention;

[0051] Figure 4 : A strontium-calcium ratio diagram of an otolith provided in an embodiment of the present invention;

[0052] Figure 5 : A schematic diagram of the structure of a device for analyzing the migratory life history of Coilia striata provided by an embodiment of the present invention;

[0053] Figure 6 : A specific connection flow chart of a device for analyzing the migratory life history of Coilia striata provided by an embodiment of the present invention;

[0054] Among them, 201: pressure head; 202: hard mold; 203: acrylic block; 204: otolith of Coilia spheniscus; 205: sample hole; 301: otolith grinding slice acquisition module; 302: element distribution curve acquisition module; 303: life history analysis module; 3011: otolith grinding slice acquisition unit; 30111: otolith embedding subunit; 30112: otolith grinding and polishing subunit; 3021: element distribution curve acquisition unit; 30211: analysis parameter setting subunit; 3031: life history analysis unit. DETAILED DESCRIPTION

[0055] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0056] Embodiment 1

[0057] Please refer to Figure 1 , is a flow chart of the steps of a method for analyzing the migratory life history of Coilia spp. provided in an embodiment of the present invention, including steps S101-S103.

[0058] Step S101: According to a preset otolith processing method, the initial otoliths are cleaned, embedded, ground and polished to obtain a smooth otolith grinding disc.

[0059] In this embodiment, the initial otoliths are cleaned, embedded, ground and polished according to the preset otolith processing method to obtain a smooth otolith grinding disc, which specifically includes:

[0060] collecting initial otoliths, and cleaning the initial otoliths to obtain cleaned otoliths;

[0061] The cleaned otoliths are embedded according to the preset curing material to obtain an otolith acrylic block;

[0062] According to the preset grinding tools and suspension, the otolith acrylic block is ground and polished in sequence to obtain a smooth otolith grinding disc.

[0063] By selecting specific curing materials, the time required for the embedding process is effectively reduced and the embedding cost is reduced; at the same time, the use of preset grinding tools and suspensions significantly improves the quality of the abrasive discs and the efficiency of grinding and polishing.

[0064] In an optional embodiment, please refer to Table 1, which is a body length and weight table of five silver anchovies collected from the Dongguan waters of the Pearl River provided in an embodiment of the present invention, wherein five silver anchovies are collected and numbered, and the left and right sagittal otoliths are removed from the inner ears of the silver anchovies using tweezers and scissors. When clamping, the edges of the otoliths should be avoided from breaking, so as to obtain initial otoliths.

[0065] Table 1

[0066]

[0067] In an alternative embodiment, please refer to Figure 2, is a schematic diagram of the overall appearance structure of an otolith provided by an embodiment of the present invention. Ultrapure water is prepared using a MilliQ pure water system, and the ultrapure water is used to clean the mucus and capsule attached to the outside of the otolith for 3 minutes, and then alcohol is used to clean the organic matter on the surface of the otolith for 2 minutes. The cleaning is completed until no residual substances are observed under a microscope. The cleaned otoliths are distinguished into left and right, photographed using a stereo microscope, and placed in a marked centrifuge tube, dried at room temperature, and the left otoliths are uniformly picked as the cleaned otoliths.

[0068] It should be noted that using the MilliQ pure water system to produce ultrapure water is a common operation for cleaning otoliths.

[0069] In this embodiment, the cleaned otoliths are embedded according to the preset solidifying material to obtain the otolith acrylic block, which specifically includes:

[0070] Place the cleaned otoliths in a preset mold dripped with preset glue;

[0071] According to a preset weight ratio, the acrylic powder and the acrylic curing agent are mixed and stirred, and poured into a preset mold to embed the cleaned otoliths;

[0072] After the preset embedding time, an acrylic block of otoliths is obtained.

[0073] By using acrylic specially for cold mounting, the time and cost required for embedding are greatly shortened, thereby improving the efficiency of obtaining otolith grinds.

[0074] In an optional embodiment, an alcohol lamp is used to mix AB glue on aluminum foil and heat it until it melts, AB glue is dripped on the bottom of the soft mold, and the cleaned otolith is placed on the AB glue with the convex surface facing downward and the concave surface facing upward, and the grinding surface is parallel to the ground. After waiting for the AB glue to be completely cured, the acrylic powder and the acrylic curing agent are weighed and mixed according to a weight ratio of 10:8, and then stirred evenly with a stirring rod at a speed of 40-50 turns / minute, and the stirred acrylic mixture is poured into the mold; after 15 minutes, the otolith is taken out and labeled to obtain an otolith acrylic block, wherein the inner diameter of the mold is 25mm and the height is 18mm.

[0075] In this embodiment, the otolith acrylic block is ground and polished in sequence according to the preset grinding tool and suspension to obtain a smooth otolith grinding disc, which specifically includes:

[0076] Place the otolith acrylic block on the preset grinding tool and mark the core of the otolith in the otolith acrylic block;

[0077] Using the first water-abrasive sandpaper and the second water-abrasive sandpaper in sequence to grind the single side of the otolith acrylic block until the core of the otolith in the otolith acrylic block is exposed, thereby obtaining a rough otolith grind sheet;

[0078] The preset first alumina suspension and the second alumina suspension are obtained, and the rough otolith grinding disc is polished in combination with the gradient particle size polishing method to obtain a smooth otolith grinding disc.

[0079] By using only two different mesh sizes of water-abrasive sandpaper for single-sided grinding, compared with the prior art of using multiple mesh sizes of water-abrasive sandpaper for double-sided grinding, the types of sandpaper mesh sizes and grinding steps are greatly reduced, and the grinding efficiency is significantly improved; at the same time, combining two different particle sizes of aluminum oxide suspensions with the gradient particle size polishing method effectively reduces scratches on the surface of the grinding wheel, thereby improving the overall quality of the grinding wheel.

[0080] In an alternative embodiment, please refer to Figure 3 , is a schematic diagram of a structure of fixing otoliths on a semi-automatic grinding and polishing machine provided by an embodiment of the present invention, wherein the structure includes: a pressure head 201, a hard mold 202, an acrylic block 203, an otolith of anchovy 204 and a sample hole 205;

[0081] The otolith acrylic block is fixed with a hard mold 202, the near-core grinding position of the otolith is marked on the otolith acrylic block, and 600-mesh water-abrasive sandpaper is used for batch automatic rough grinding on a semi-automatic grinder and polisher; the grinding parameters of the semi-automatic grinder and polisher are designed as a pressure of 25N, a grinding and polishing head speed of 60r / min, a chassis speed of 200r / min, and the grinding and polishing head is opposite to the chassis; the grinding is stopped when the marked position is ground; then 1200-mesh water-abrasive sandpaper is used for manual grinding on the grinder and polisher, the chassis speed is 50r / min, and the degree of grinding is observed under a stereo microscope every 10s of grinding until the core is exposed and the grinding is completed.

[0082] It should be noted that the semi-automatic grinding and polishing machine may be an AutoMet 250pro grinding and polishing machine.

[0083] In an optional embodiment, the grinding disc of the semi-automatic grinder and polisher is replaced with a polishing disc equipped with a fine polishing cloth, and a 70-90 g / ml alumina suspension is prepared using a 15 ml centrifuge tube; thereafter, a 3 μm alumina suspension is used for preliminary batch automatic polishing for 10 minutes, and then a 0.3 μm alumina suspension is used for batch automatic polishing for 5 minutes until there are no scratches on the surface of the grinding disc.

[0084] Step S102: Obtaining an element distribution curve according to a preset analysis technique in combination with a smooth otolith grind.

[0085] In this embodiment, the element distribution curve is obtained according to the preset analysis technology in combination with the smooth otolith grinding disc, specifically including:

[0086] The smooth otolith grinding disc is cleaned and coated to obtain the otolith grinding disc for analysis;

[0087] Using a preset electron probe micro-area analyzer and combining preset analysis parameters, the scanning line of the otolith grinding piece used for analysis is scanned to obtain an element distribution curve.

[0088] By using an electron probe microanalyzer and preset analysis parameters, the scanning time is effectively shortened and the cost is reduced, while ensuring the accuracy of the element distribution curve.

[0089] In an optional embodiment, the cleaning and coating of the smooth otolith grinding disc specifically includes:

[0090] The ground and polished otolith disc was placed in ultrapure water produced by the MilliQ pure water system for ultrasonic cleaning for 5 minutes, then covered with a petri dish and dried at room temperature for 12 hours; after being completely dried, a high vacuum evaporator was used to evaporate a carbon film to obtain an otolith disc for analysis.

[0091] In this embodiment, as a preferred solution, the preset analysis parameters specifically include:

[0092] The accelerating voltage is 15 kV and the electron beam current is 5 × 10 -8 A, beam spot diameter is 1 μm, dwell time at each point is 500 ms, and dot interval is 1 μm.

[0093] This preferred solution uses an accelerating voltage of 15 kV to ensure that the analyzer can have a higher detection limit and a lower standard deviation. -8 A's electron beam current can ensure that the excitation capacity is increased while the degree of otolith damage is low. The beam spot diameter and dot interval of 1μm can increase the number of dots and shorten the dot interval to ensure the continuity and accuracy of the data and avoid the loss of environmental information due to the large dot interval. The dot efficiency can be improved through the 500ms dwell time.

[0094] Step S103: Analyze the migratory life history of Coilia spp. according to the element distribution curve.

[0095] In this embodiment, the migratory life history of Coilia spheniscus is analyzed according to the element distribution curve, specifically including:

[0096] According to the preset drawing library, a line graph was constructed with the distance from the otolith core as the horizontal axis and the element content ratio as the vertical axis, and the migratory life history of the silver carp was analyzed based on the line graph.

[0097] Through the drawing library, the longest radius of the otolith and the element content ratio are combined to construct a line graph, which enables an intuitive analysis of the migratory life history of the golden anchovy.

[0098] In an optional embodiment, the preset drawing library may be Python's Matplotlib library, which constructs a line graph with the distance from the otolith core as the horizontal coordinate and Sr / Ca×1000 as the vertical coordinate, and analyzes the migration history of the anchovy in freshwater, estuaries and seawater based on the line graph.

[0099] In an alternative embodiment, please refer to Figure 4 , is a graph of strontium-calcium ratios of otoliths provided by an embodiment of the present invention, wherein the Sr / Ca×1000 range of freshwater environment is 0-3, the Sr / Ca×1000 range of brackish water environment is 3-7, and the Sr / Ca×1000 of seawater environment is greater than 7. Figure 4 As shown, the spawning grounds of the five anchovies of this embodiment are all in freshwater areas, among which the data of individuals numbered F02, F04 and F05 fluctuate in the range of 1-3, indicating that there is migration behavior from freshwater to near-fresh and salty water, which is a semi-migratory type; the data of individuals numbered F01 and F03 fluctuate only in the range of 1-2.5, and they are only active in freshwater areas without obvious migration behavior, which is a sedentary type.

[0100] In an optional embodiment, the process of constructing a line graph includes: reading target parameters of Ca and Sr, the target parameters include: root path, file name and sample name; then automatically obtaining the file path according to the path function, the path function includes: function get_target_Ca_filelist() and function get_target_Sr_filelist(); then defining the data structure according to the structure definition function, and using the data processing function to process the data, the structure definition function includes: create_group_data_structure(); the process of processing the data includes: reading data, extracting horizontal and vertical coordinate data and calculating and returning the calculation results; the data processing function includes: calculate_SrCa(); finally, using the subplots() function in the matplotlib.pyplot library to merge and draw, the process of merging and drawing includes: creating a canvas, displaying sub-graphs, setting the horizontal and vertical coordinate labels, scales, ranges and titles of the sub-graphs, adding freshwater and saltwater boundary lines, adjusting the sub-graph spacing and displaying the merged graph.

[0101] In an optional embodiment, the process of constructing a line chart also includes: importing a function library, defining global variables and functions, and executing main logic code in a code block; the function library includes: os, matplotlib.pyplot, pandas and numpy, and the global variables include: path, target_Ca_file_name, target_Sr_file_name, sample_names; the functions include: get_target_Ca_filelist(), get_target_Sr_filelist(), create_group_data_structure() and calculate_SrCa(); the execution of the main logic code in the code block includes: executing the main logic in the if_name_==”_mai n_” block.

[0102] In an optional embodiment, the process of constructing a line graph further includes: using os.wa lk to traverse the folder to obtain the file path, using pandas to read and save the data file, using matplotlib.pyplot to draw the graph, and using numpy to perform numerical calculations.

[0103] This embodiment cleans, embeds, grinds and polishes the otoliths. By improving the embedding, grinding and polishing processes, the efficiency and quality of the otolith grinding discs are improved. By analyzing the element content of the otolith grinding discs through analytical technology, the element distribution curve with smaller error can be obtained more quickly and accurately. Based on the element distribution curve, the migratory life history of the anchovy can be deeply analyzed, and the migratory life history of the anchovy can be analyzed efficiently and accurately based on the otoliths. This not only provides a scientific basis for the management of anchovy breeding and the sustainable utilization of fishery resources, but also further contributes important data to the protection and development of fishery resources of anchovy.

[0104] Embodiment 2

[0105] Please refer to Figure 5 , is a schematic diagram of the structure of an analysis device for the migratory life history of Coilia striata provided by an embodiment of the present invention, comprising: an otolith grinding slice acquisition module 301, an element distribution curve acquisition module 302 and a life history analysis module 303;

[0106] Please refer to Figure 6, which is a specific connection flow chart of an analysis device for the migratory life history of Coilia striata provided in an embodiment of the present invention, including: an otolith grinding slice acquisition module 301, an element distribution curve acquisition module 302, a life history analysis module 303, an otolith grinding slice acquisition unit 3011, an otolith embedding subunit 30111, an otolith grinding and polishing subunit 30112, an element distribution curve acquisition unit 3021, an analysis parameter setting subunit 30211 and a life history analysis unit 3031.

[0107] The otolith grinding disc acquisition module 301 is used to clean, embed, grind and polish the initial otoliths according to a preset otolith processing method to obtain a smooth otolith grinding disc.

[0108] In this embodiment, the otolith grinding piece acquisition module 301 includes: an otolith grinding piece acquisition unit 3011;

[0109] The otolith grinding piece obtaining unit is used to collect initial otoliths, clean the initial otoliths, and obtain cleaned otoliths;

[0110] The cleaned otoliths are embedded according to the preset curing material to obtain an otolith acrylic block;

[0111] According to the preset grinding tools and suspension, the otolith acrylic block is ground and polished in sequence to obtain a smooth otolith grinding disc.

[0112] In this embodiment, the otolith grinding piece obtaining unit 3011 includes: an otolith embedding subunit 30111;

[0113] The otolith embedding subunit 30111 is used to place the cleaned otoliths in a preset mold dripped with preset glue;

[0114] According to a preset weight ratio, the acrylic powder and the acrylic curing agent are mixed and stirred, and poured into a preset mold to embed the cleaned otoliths;

[0115] After the preset embedding time, an acrylic block of otoliths is obtained.

[0116] In this embodiment, the otolith grinding piece obtaining unit includes: an otolith grinding and polishing subunit 30112; the otolith grinding and polishing subunit 30112 is used to place the otolith acrylic block on a preset grinding tool and mark the core of the otolith in the otolith acrylic block;

[0117] Using the first water-abrasive sandpaper and the second water-abrasive sandpaper in sequence to grind the single side of the otolith acrylic block until the core of the otolith in the otolith acrylic block is exposed, thereby obtaining a rough otolith grind sheet;

[0118] The preset first alumina suspension and the second alumina suspension are obtained, and the rough otolith grinding disc is polished in combination with the gradient particle size polishing method to obtain a smooth otolith grinding disc.

[0119] The element distribution curve acquisition module 302 is used to obtain the element distribution curve according to a preset analysis technology in combination with a smooth otolith grinding disc.

[0120] In this embodiment, the element distribution curve acquisition module 302 includes: an element distribution curve acquisition unit 3021;

[0121] The element distribution curve acquisition unit is used to clean and coat the smooth otolith grinding piece to obtain the otolith grinding piece for analysis;

[0122] Using a preset electron probe micro-area analyzer and combining preset analysis parameters, the scanning line of the otolith grinding piece used for analysis is scanned to obtain an element distribution curve.

[0123] In this embodiment, the element distribution curve acquisition unit includes: an analysis parameter setting subunit 30211;

[0124] The analysis parameter setting subunit is used to set the preset analysis parameters, which specifically include: the acceleration voltage is 15 kV, the electron beam current is 5×10 -8 A, beam spot diameter is 1 μm, dwell time at each point is 500 ms, and dot interval is 1 μm.

[0125] The life history analysis module 303 is used to analyze the migratory life history of Coilia spheniscus according to the element distribution curve.

[0126] In this embodiment, the life history analysis module 303 includes: a life history analysis unit 3031;

[0127] The life history analysis unit 3031 is used to construct a line graph according to a preset drawing library, with the distance from the otolith core as the horizontal axis and the element content ratio as the vertical axis, and analyze the migratory life history of the golden anchovy based on the line graph.

[0128] In this embodiment, the otoliths are cleaned, embedded, ground and polished. Through the improvements in the embedding, grinding and polishing processes, the preparation efficiency and the quality of the otolith grind are significantly improved; and the element content of the otolith grind is analyzed by analytical technology in combination with improved analytical parameters. Under the premise of ensuring the continuity and accuracy of the dot data, the element distribution curve with smaller error is obtained more quickly and accurately, and then based on the ratio of the longest radius of the otolith to the element content in the element distribution curve, the migratory life history of the silver anchovy is intuitively analyzed, and efficient and accurate analysis of the migratory life history of the silver anchovy based on the otoliths is achieved, which provides a strong scientific basis for analyzing the migratory life history of the silver anchovy, provides valuable data support for the breeding management, ecological research and fishery resource protection of the silver anchovy, and effectively enriches the research and application scope of my country's fishery resources.

[0129] In summary, the present embodiment embeds, grinds and polishes the otoliths, and improves the efficiency and quality of the otolith grinding discs by improving the embedding, grinding and polishing processes; and by analyzing the element content of the otolith grinding discs through analytical technology, it is possible to obtain an element distribution curve with a smaller error more quickly and accurately, and then analyze the migratory life history of the silver anchovy based on the element distribution curve, making the study of the migratory life history of the silver anchovy based on otoliths more reliable, contributing important data to the protection and development of the fishery resources of the silver anchovy, and providing a scientific basis for the breeding management of the silver anchovy and the sustainable utilization of fishery resources.

[0130] The specific embodiments described above further illustrate the purpose, technical solutions and beneficial effects of the present invention. It should be understood that the above description is only a specific embodiment of the present invention and is not intended to limit the scope of protection of the present invention. It is particularly pointed out that for those skilled in the art, any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the scope of protection of the present invention.

Claims

1. A method for analyzing the migratory life history of Coilia striata, characterized in that: include: Step S101: According to a preset otolith processing method, the initial otoliths are cleaned, embedded, ground and polished to obtain a smooth otolith grinding disc; Step S102: Obtaining an element distribution curve according to a preset analysis technique in combination with a smooth otolith grind; Step S103: Analyze the migratory life history of Coilia spp. according to the element distribution curve.

2. The method for analyzing the migratory life history of Coilia fasciatus according to claim 1, characterized in that: The method of cleaning, embedding, grinding and polishing the initial otoliths according to the preset otolith processing method to obtain a smooth otolith grinding sheet specifically includes: collecting initial otoliths, and cleaning the initial otoliths to obtain cleaned otoliths; The cleaned otoliths are embedded according to the preset curing material to obtain an otolith acrylic block; According to the preset grinding tools and suspension, the otolith acrylic block is ground and polished in sequence to obtain a smooth otolith grinding disc.

3. The method for analyzing the migratory life history of Coilia fasciatus according to claim 2, characterized in that: The method of embedding the cleaned otoliths according to the preset solidifying material to obtain the otolith acrylic block specifically includes: Place the cleaned otoliths in a preset mold dripped with preset glue; According to a preset weight ratio, the acrylic powder and the acrylic curing agent are mixed and stirred, and poured into a preset mold to embed the cleaned otoliths; After the preset embedding time, an acrylic block of otoliths is obtained.

4. The method for analyzing the migratory life history of Coilia fasciatus according to claim 2, characterized in that: The method of grinding and polishing the otolith acrylic block in sequence according to the preset grinding tool and suspension to obtain a smooth otolith grinding sheet specifically includes: Place the otolith acrylic block on the preset grinding tool and mark the core of the otolith in the otolith acrylic block; Using the first water-abrasive sandpaper and the second water-abrasive sandpaper in sequence to grind the single side of the otolith acrylic block until the core of the otolith in the otolith acrylic block is exposed, thereby obtaining a rough otolith grind sheet; The preset first alumina suspension and the second alumina suspension are obtained, and the rough otolith grinding disc is polished in combination with the gradient particle size polishing method to obtain a smooth otolith grinding disc.

5. The method for analyzing the migratory life history of Coilia fasciatus according to claim 1, characterized in that: The element distribution curve is obtained according to the preset analysis technology in combination with the smooth otolith grinding piece, which specifically includes: The smooth otolith grinding disc is cleaned and coated to obtain the otolith grinding disc for analysis; Using a preset electron probe micro-area analyzer and combining preset analysis parameters, the scanning line of the otolith grinding piece used for analysis is scanned to obtain an element distribution curve.

6. The method for analyzing the migratory life history of Coilia fasciatus according to claim 5, characterized in that: The preset analysis parameters specifically include: The accelerating voltage is 15 kV and the electron beam current is 5 × 10 -8 A, beam spot diameter is 1 μm, dwell time at each point is 500 ms, and dot interval is 1 μm.

7. The method for analyzing the migratory life history of Coilia fasciatus according to claim 1, characterized in that: The migratory life history of Coilia spheniscus is analyzed according to the element distribution curve, specifically including: According to the preset drawing library, a line graph was constructed with the distance from the otolith core as the horizontal axis and the element content ratio as the vertical axis, and the migratory life history of the silver carp was analyzed based on the line graph.

8. An analytical device for the migratory life history of Coilia striata, characterized in that: include: Otolith grinding acquisition module, element distribution curve acquisition module and life history analysis module; The otolith grinding disc acquisition module is used to clean, embed, grind and polish the initial otoliths according to a preset otolith processing method to obtain a smooth otolith grinding disc; The element distribution curve acquisition module is used to obtain the element distribution curve according to a preset analysis technique in combination with a smooth otolith grinding disc; The life history analysis module is used to analyze the migratory life history of Coilia spheniscus according to the element distribution curve.

9. The device for analyzing the migratory life history of Coilia fasciatus according to claim 8, characterized in that: The otolith grinding piece acquisition module includes: an otolith grinding piece acquisition unit; The otolith grinding piece obtaining unit is used to collect initial otoliths, clean the initial otoliths, and obtain cleaned otoliths; The cleaned otoliths are embedded according to the preset curing material to obtain an otolith acrylic block; According to the preset grinding tools and suspension, the otolith acrylic block is ground and polished in sequence to obtain a smooth otolith grinding disc.

10. The device for analyzing the migratory life history of Coilia fasciatus according to claim 9, characterized in that: The otolith grinding piece obtaining unit comprises: an otolith embedding subunit; The otolith embedding subunit is used to place the cleaned otoliths in a preset mold dripped with preset glue; According to a preset weight ratio, the acrylic powder and the acrylic curing agent are mixed and stirred, and poured into a preset mold to embed the cleaned otoliths; After the preset embedding time, an acrylic block of otoliths is obtained.

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