Hard wood cutting breeding method for actinidia arguta

Abstract

The invention provides a hard wood cutting breeding method for actinidia arguta, and relates to the technical field of asexual propagation seedling raising, and the method comprises the following steps: (1) collecting 1-2-year-old branches, and pruning the branches; (2) the cuttings are cut into 10cm cuttings with more than two bud points, the upper openings of the cuttings are flat, the lower openings of the cuttings are inclined, and the cuttings are soaked in carbendazim for sterilization treatment; (3) selecting grass carbon mixed perlite as a cutting medium, putting the cutting medium into a plug tray, and disinfecting the plug tray; (4) preparing a rooting agent, treating the cutting slips in the step (2), inserting the cutting slips into a plug tray substrate, thoroughly watering the cutting slips with carbendazim after fixing, and covering the cutting slips with a transparent cover; (5) after cuttage, placing under the condition of 70% shading degree, increasing 20% illumination and controlling the temperature to be 10-30 DEG C after new leaves emerge, supplementing water in time after cuttage, sterilizing in combination with carbendazim, opening a transparent cover step by step in 40 days of cuttage, watering according to conditions, and spraying a foliar fertilizer every 10 days; and (6) 60 days after cuttage, transplanting and planting. The method has the advantages that the actinidia arguta hardwood cutting step is simplified, and the cutting breeding period is shortened.

Description

Technical Field

[0001] The invention provides a hard branch cutting propagation method for Actinidia rapa kiwifruit, and relates to the technical field of asexual propagation and seedling raising. Background Art

[0002] Actinidia polygama, also known as wood Tian Liao, belongs to the genus Actinidia of the family Actinidia, and is a rare plant in Shandong Province. Actinidia polygama has high medicinal value. Domestic and foreign scholars have conducted many studies on its medicinal components. It can be used to make health drinks and cosmetics. It has strong stress resistance and good waterlogging resistance. It can also be used as a kiwi rootstock, and has good application prospects.

[0003] At present, there is little research data on the breeding of Actinidia gezaoensis, mainly seed seedling, tissue culture propagation and cutting propagation. Seed seedling is small (1000-grain weight is only 1.3g), and it needs to be treated with low temperature for 40 to 50 days before sowing. The seedbed must be carefully prepared, and it needs to be shaded during the seedling stage and maintain high field humidity. The maintenance conditions are relatively high. Tissue culture propagation and cutting propagation are both asexual propagation techniques, which have the excellent characteristics of maintaining the mother plant. Li Guijun et al., Liu Yongli et al. studied the tissue culture rapid propagation system of Actinidia gezaoensis. The superior plant 'Zhemizhen No. 1' with good waterlogging resistance was propagated through tissue culture, but tissue culture propagation requires good experimental conditions and operation techniques, and has high requirements for environmental facilities and personnel.

[0004] Cutting propagation is currently widely used in plant production, but there are few public literature on the cutting propagation of Actinidia gezao. Among them, the results of Li Hongli's research on hardwood cutting propagation technology showed that the best rooting effect was achieved when fine river sand was used as the cutting medium and the base of the cuttings was soaked in 200mg / LABT for 30 minutes. Adventitious roots began to occur after 57 days, and the rooting rate reached more than 90%. However, due to the long rooting time, the new shoots of the transplanted seedlings were basically immature in the same year, and the buds were not full, so the survival rate of returning to the nursery in the second year was very low; Mu Yushan et al. used 1 / 2 perlite + 1 / 2 fine river sand as the matrix for hardwood cuttings, soaked in 100mg / LABT rooting powder solution for 24 hours or soaked in 500mg / LABT Soak in the solution for 3 hours, spray once every 10 to 15 minutes in the initial stage of cutting under full-light spray conditions, and rotate 2 weeks each time. When the temperature rises and the spikelets unfold their leaves, spray once every 5 to 7 minutes, and rotate 3 weeks each time. The cutting survival rate is above 80.1%. This technology requires the installation of full-light fog equipment and a timing device, and the literature does not mention the rooting time; Song Guiquan uses young branches as materials and river sand as the matrix, and soaks them in 4000 to 5000 mg / kg of indolebutyric acid solution for 5 minutes. It can take about 1 month to take root. The cutting method described in the literature is relatively general and does not mention the survival rate. Summary of the invention

[0005] The invention provides a hard branch cutting propagation method for Actinidia rapa, which solves the technical problems in the prior art that the rooting time of Actinidia rapa cuttings is long, the propagation cycle is long, and the cutting propagation steps are complicated.

[0006] The present invention is achieved by comprising the steps of:

[0007] (1) Collect 1-2 year old branches and prune the branches;

[0008] (2) cutting cuttings with a length of 10 cm and having more than two buds, wherein the cuttings have a flat top and a slanted bottom, and immersing the cuttings in a sterilizing solution for sterilization;

[0009] (3) Select peat mixed with perlite as the cutting medium, put it into the plug tray and disinfect it;

[0010] (4) soaking the cuttings of step (2) in a rooting agent and inserting them into the substrate of step (3), watering them thoroughly with a sterilizing solution after they are fixed, and covering them with a transparent cover to complete the cuttings; covering them with a transparent cover can play a role in heat preservation and moisture retention.

[0011] (5) After cutting, place under 70% shading conditions. After the new leaves are unfolded, increase the light by 20% and control the temperature at 10℃~30℃. Rehydrate in time after cutting and sterilize with bactericidal liquid. After 40 days of cutting, gradually open the transparent cover, water as needed, and spray foliar fertilizer every 10 days.

[0012] (6) Transplant and plant 60 days after cutting.

[0013] As a further preference, the mass ratio of peat to perlite is 4:1.

[0014] As further preferred, the rooting agent includes 700 mg / LIBA+300 mg / LNAA.

[0015] As a further preference, the rooting agent soaking method is to soak the base of the cuttings 3 cm for 3 minutes.

[0016] As a further preference, water is replenished once every 5 days after cutting, and sterilization is carried out by spraying a sterilizing liquid every 15 days in combination with water replenishment.

[0017] As a further preference, the bactericidal solution is a 1000-fold solution of 50% carbendazim wettable powder.

[0018] As a further preference, the foliar fertilizer used in step (5) is a 0.1% potassium dihydrogen phosphate / urea solution.

[0019] The beneficial effects of the present invention are that, in combination with the cutting characteristics of Actinidia gezaoensis, a composite rooting agent is selected, a cutting medium is limited, the cutting steps are simplified, the rooting time of the cuttings is shortened, and thus the breeding cycle is shortened. The rooting time of the cuttings is short, and the cuttings begin to take root 25 days after the cuttings, and the rooting efficiency is more than 95%. The breeding cycle is short, and the cuttings can be transplanted into the nursery 60 days after the cuttings, and can be transplanted in the same year. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a cutting effect diagram of Example 1;

[0021] Figure 2 This is a rendering of the plug tray covered with a transparent cover after cutting in Example 1;

[0022] Figure 3 This is a diagram of the rooting situation of the cuttings of Example 1 at 25 days;

[0023] Figure 4 This is a schematic diagram of the survival of cuttings 60 days after cutting in Example 1;

[0024] Figure 5 This is a schematic diagram of the roots of the cuttings 60 days after cutting in Example 1;

[0025] Figure 6 This is a schematic diagram of the root system after cleaning the soil at the root of the cuttings 60 days after cutting in Example 1;

[0026] Figure 7 This is a schematic diagram of the root system of the cuttings 60 days after cutting in Example 1. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] Example 1

[0029] The hard branch cutting propagation method of Actinidia gezaoensis comprises the following steps:

[0030] 1. Strip selection and pretreatment

[0031] In Jinan, Shandong, when the buds of Actinidia zedoaria begin to sprout in late March, mother plants with good growth are selected, and 1-2 year old strong branches are collected, and branches with a thickness of less than 0.2 cm are cut off.

[0032] 2. Cuttings cutting and disinfection

[0033] The pretreated branches were cut into cuttings with a length of about 10 cm and more than 2 buds. The upper end of the cuttings was cut flat and the lower end was cut into a horseshoe-shaped oblique end. 50 cuttings were bundled and placed in a 1000-fold solution of carbendazim (50% wettable powder) for 5 minutes for disinfection.

[0034] 3. Preparation of cutting medium and rooting agent

[0035] Use peat: perlite = 4:1 as the cutting medium, and use 1000 times diluted carbendazim (50% wettable powder) to disinfect after placing it in the plug tray; prepare a compound hormone solution of 700mg / L IBA + 300mg / L NAA as a rooting agent, and store the rooting agent solution at 5℃ in the dark for later use.

[0036] 4. Cuttings

[0037] Place the cuttings treated in step 2 in the rooting agent solution and soak them at a depth of 3 cm from the base of the cuttings. Take them out after 3 minutes of treatment, and then make a 4 cm deep and 0.5 cm diameter cutting hole in the middle of each cutting position in the plug tray. Figure 1 , put the treated cuttings into the hole, fix the cuttings, and water them thoroughly with 1000 times dilution of carbendazim (50% wettable powder). Figure 2 , cover with a transparent cover to keep warm and moisturize, and cover each hole tray with a transparent cover.

[0038] 5. Management after cutting

[0039] After cutting, place the cutting tray under 70% shading conditions, and increase the light by 20% after the new leaves are unfolded; during the cutting period, the night temperature should not be lower than 10℃, the daytime temperature should not be higher than 30℃, and the substrate moisture content should be 60%. Open the cover every 5 days to check the rooting situation and add water once, and apply 1000 times dilution of carbendazim (50% wettable powder) every 15 days to sterilize. Figure 3 , 25 days after cutting, the cuttings have taken root. 40 days after cutting, gradually open the transparent cover until it is completely removed. After removing the transparent cover, water once every 2 to 3 days according to the degree of dryness and wetness, and alternately spray 0.1% potassium dihydrogen phosphate / urea solution once every 10 days for foliar topdressing. Figure 4 , Figure 5 , Figure 6 and Figure 7 , 60 days after cutting, the seedling height, root length, root type and number, rooting rate and other indicators were investigated. The data are shown in Table 1.

[0040] Table 1 Example 1 Rooting of Actinidia zedoaria cuttings

[0041]

[0042] As shown in Table 1, the cuttings began to take root 25 days after cutting, with an average seedling height of 46.56 cm and a root length of 9.19 cm. The rooting type was a mixed rooting type of callus rooting and cortex rooting, and the final cutting rooting rate was 95%.

[0043] 6 Transplanting

[0044] After 60 days of cutting, the root system is well developed and can be transplanted to 20cm-30cm seedling pots to cultivate large-sized seedlings, or transplanted to resource areas in the rainy season with a planting density of 200-400 plants / mu, and the seedlings can be transplanted in the same year. The survival rate of transplanting to seedling pots is more than 99%, and the survival rate of transplanting to the field is more than 90%.

[0045] Comparative Example 1

[0046] Compared with Example 1, the difference of Comparative Example 1 is that the cutting medium used in step 3 is river sand, and the other operations are the same. After 60 days of cutting, the seedling height, root length, root type and number, rooting rate and other indicators were investigated. The investigation results are shown in Table 2.

[0047] Table 2 Comparative Example 1 Rooting of Actinidia zedoaria cuttings

[0048]

[0049] As shown in Table 2, the cuttings of Comparative Example 1 began to take root 25 days after cutting, with an average seedling height of 19.02 cm and a root length of 9.95 cm. The rooting type was a mixed rooting type of callus rooting and cortex rooting, and the final cutting rooting rate was 90%. Compared with Example 1, the rooting rate of Comparative Example 1 was acceptable, but the growth of the seedlings was significantly poor.

[0050] Comparative Example 2

[0051] Compared with Example 1, the difference of Comparative Example 2 is that the hormone used in step 3 is replaced by clean water, and the other operations are the same. After 60 days of cutting, the seedling height, root length, root type and number, rooting rate and other indicators were investigated. The investigation results are shown in Table 3.

[0052] Table 3 Comparative Example 2 Rooting of Actinidia zedoaria cuttings

[0053]

[0054] As shown in Table 3, the cuttings began to take root 30 days after cutting, with an average seedling height of 22.23 cm and a root length of 9.38 cm. The rooting type was a mixed rooting type of callus rooting and cortex rooting, and the final cutting rooting rate was 79.2%. Compared with Example 1, the growth and rooting rate of the cuttings in Comparative Example 2 were poor.

[0055] The beneficial effects of the present invention are that, in combination with the cutting characteristics of Actinidia gezaoensis, a composite rooting agent is selected, a cutting medium is limited, the cutting steps are simplified, the rooting time of the cuttings is shortened, and thus the breeding cycle is shortened. The rooting time of the cuttings is short, and the cuttings begin to take root 25 days after the cuttings, and the rooting efficiency is more than 95%. The breeding cycle is short, and the cuttings can be transplanted into the nursery 60 days after the cuttings, and can be transplanted in the same year.

[0056] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A hard branch cutting propagation method for Actinidia gezaoensis, characterized in that: The steps include: (1) Collect 1-2 year old branches and prune the branches; (2) cuttings with a length of 10 cm and having two or more buds, wherein the cuttings have a flat top and a slanted bottom, and immersing them in a sterilizing solution for sterilization; (3) Select peat mixed with perlite as the cutting medium, put it into the plug tray and disinfect it; (4) soaking the cuttings of step (2) in a rooting agent and inserting them into the substrate of step (3), watering them thoroughly with a sterilizing solution after they are fixed, and covering them with a transparent cover to complete the cuttings; (5) After cutting, place under 70% shading conditions. After the new leaves are unfolded, increase the light by 20% and control the temperature at 10℃~30℃. Rehydrate in time after cutting and sterilize with bactericidal liquid. After 40 days of cutting, gradually open the transparent cover, water as needed, and spray foliar fertilizer every 10 days. (6) Transplant and plant 60 days after cutting.

2. The hard branch cutting propagation method of Actinidia puerariae according to claim 1, characterized in that: The mass ratio of the peat to perlite is 4:

1.

3. The hard branch cutting propagation method of Actinidia rapa according to claim 1, characterized in that: The rooting agent includes 700 mg / LIBA+300 mg / LNAA.

4. The hard branch cutting propagation method of Actinidia rapa according to claim 3, characterized in that: The method of soaking the rooting agent is to soak the base of the cuttings 3cm for 3 minutes.

5. The hard branch cutting propagation method of Actinidia puerariae according to claim 1, characterized in that: After cutting, add water once every 5 days, and apply fungicide every 15 days in combination with watering.

6. The hard branch cutting propagation method of Actinidia puerariae according to claim 1, characterized in that: The bactericidal liquid is a 1000-fold solution of 50% carbendazim wettable powder.

7. The hard branch cutting propagation method of Actinidia puerariae according to claim 1, characterized in that: The foliar fertilizer used in step (5) is a 0.1% potassium dihydrogen phosphate / urea solution.

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