Composition for attracting fall armyworm and its application

Abstract

The present invention relates to a composition for attracting fall armyworm and its application. The active components in the composition for attracting fall armyworm include eucalyptol, ALPHA-pinene and D-limonene. In addition, verbenol can enhance the active components.

Description

Technical Field

[0001] The invention relates to the field of odor compounds, and in particular to a combination for attracting fall armyworm and application thereof. Background Art

[0002] Fall Armyworm Spodoptera frugiperda ) is a major invasive pest with a particularly wide range of host plants. It can feed on more than 76 families and more than 350 species of plants. Its larvae can eat a large number of crop leaves, causing serious economic losses. Among them, the fall armyworm is extremely harmful to corn and can cause a large-scale reduction in corn production. The female fall armyworm has a strong egg-laying ability, and each female can lay about 1,500 eggs in its lifetime. At present, most methods for preventing and controlling fall armyworms are chemical control, but in recent years, reports have begun to appear that fall armyworms have developed resistance to pesticides. At the same time, chemical pesticides will bring a series of problems such as environmental pollution and pesticide residues. In addition, the resistance of fall armyworms to Bacillus thuringiensis biocontrol agents has also been reported. It can be seen that traditional methods, whether chemical pesticides or genetically modified crops, will face severe challenges in controlling fall armyworms.

[0003] Screening of volatile compounds that are attractive to fall armyworm is of great significance for the development of efficient and eco-friendly attractants. Summary of the invention

[0004] One of the present inventions provides a composition for attracting fall armyworm, which comprises active components, wherein the active components include eucalyptol, ALPHA-pinene and D-limonene.

[0005] In a specific embodiment, based on the total mass of the active components as 100%, the mass of the eucalyptol accounts for 33% to 50%, the mass of the ALPHA-pinene accounts for 33% to 45%, and the mass of the D-limonene accounts for 5% to 33%.

[0006] In a specific embodiment, based on the total mass of the active components as 100%, the mass of the eucalyptol accounts for 44% to 50%, the mass of the ALPHA-pinene accounts for 40% to 45%, and the mass of the D-limonene accounts for 5% to 15%.

[0007] In a specific embodiment, the composition further comprises verbenol.

[0008] In a specific embodiment, based on the total weight of the active components as 100%, the weight amount of verbenol is 20% to 40%.

[0009] The second aspect of the present invention provides the use of the composition according to any one of the first aspects of the present invention in luring fall armyworm.

[0010] The third aspect of the present invention provides the use of verbenol as a synergist for attracting fall armyworm.

[0011] In a specific embodiment, the active ingredients for attracting fall armyworm include eucalyptol, ALPHA-pinene and D-limonene.

[0012] Beneficial effects of the present invention: The present invention finds that the combination of eucalyptol, ALPHA-pinene and D-limonene can synergistically attract fall armyworm; further, verbenol has a synergistic effect on the combination of eucalyptol, ALPHA-pinene and D-limonene. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 The number of fall armyworms captured by each bait is shown. DETAILED DESCRIPTION

[0014] The above contents of the present invention are further described in detail below in the form of preferred implementation cases, but they do not constitute a limitation to the present invention.

[0015] Unless otherwise specified, the reagents in the embodiments of the present invention can be purchased through commercial channels.

[0016] Blank desulfurized rubber stopper carrier was purchased from Zhongjie Sifang Biotechnology Co., Ltd.

[0017] Using n-hexane as solvent, verbenainol, eucalyptol, ALPHA-pinene and D-limonene were prepared into 1 μg / μl solutions respectively.

[0018] Taking n-hexane as a negative control, 100 μl of n-hexane was dripped onto a blank devulcanized rubber plug carrier of 240 mm×340 mm size to prepare a lure core, which was recorded as CK.

[0019] 100 μL of the 1 μg / μL verbena alcohol solution prepared above was added to a blank devulcanized rubber plug carrier to prepare the attractant core S1.

[0020] 100 μL of the 1 μg / μL eucalyptol solution prepared above was added to a blank devulcanized rubber plug carrier to prepare the attractant core S2.

[0021] 100 μL of the 1 μg / μL ALPHA-pinene solution prepared above was added to a blank devulcanized rubber plug carrier to prepare the attractant S3.

[0022] 100 μL of the 1 μg / μL D-limonene solution prepared above was added to a blank devulcanized rubber plug carrier to prepare the attractant S4.

[0023] 33.3 μl of the 1 μg / μl eucalyptol solution, 33.3 μl of the 1 μg / μl ALPHA-pinene solution and 33.3 μl of the 1 μg / μl D-limonene solution prepared above were added to a blank devulcanized rubber plug carrier to prepare the bait core T1.

[0024] 44 μl of the 1 μg / μl eucalyptol solution, 42 μl of the 1 μg / μl ALPHA-pinene solution and 14 μl of the 1 μg / μl D-limonene solution prepared above were added to a blank devulcanized rubber carrier to prepare the attractant T2.

[0025] 45 μl of the 1 μg / μl eucalyptol solution, 40 μl of the 1 μg / μl ALPHA-pinene solution and 15 μl of the 1 μg / μl D-limonene solution prepared above were added to a blank devulcanized rubber plug carrier to prepare the attractant T3.

[0026] 45 μl of the 1 μg / μl eucalyptol solution, 45 μl of the 1 μg / μl ALPHA-pinene solution and 10 μl of the 1 μg / μl D-limonene solution prepared above were added to a blank devulcanized rubber plug carrier to prepare the attractant T4.

[0027] 50 μl of the 1 μg / μl eucalyptol solution, 40 μl of the 1 μg / μl ALPHA-pinene solution and 10 μl of the 1 μg / μl D-limonene solution prepared above were added to a blank devulcanized rubber plug carrier to prepare the attractant T5.

[0028] 50 μl of the 1 μg / μl eucalyptol solution, 45 μl of the 1 μg / μl ALPHA-pinene solution and 5 μl of the 1 μg / μl D-limonene solution prepared above were added to a blank devulcanized rubber plug carrier to prepare the attractant T6.

[0029] 10 μl of the 1 μg / μl verbena alcohol solution, 44 μl of the 1 μg / μl eucalyptol solution, 42 μl of the 1 μg / μl ALPHA-pinene solution and 14 μl of the 1 μg / μl D-limonene solution prepared above were added to a blank devulcanized rubber plug carrier to prepare the attractant M1.

[0030] 20 μl of the 1 μg / μl verbena alcohol solution, 44 μl of the 1 μg / μl eucalyptol solution, 42 μl of the 1 μg / μl ALPHA-pinene solution and 14 μl of the 1 μg / μl D-limonene solution prepared above were added to a blank desulfurized rubber plug carrier to prepare the attractant M2.

[0031] The above-prepared 30 μl of 1 μg / μl verbena alcohol solution, 44 μl of 1 μg / μl eucalyptol solution, 42 μl of 1 μg / μl ALPHA-pinene solution and 14 μl of 1 μg / μl D-limonene solution were added to a blank desulfurized rubber plug carrier to prepare the attractant M3.

[0032] 40 μl of the 1 μg / μl verbena alcohol solution, 50 μl of the 1 μg / μl eucalyptol solution, 45 μl of the 1 μg / μl ALPHA-pinene solution and 5 μl of the 1 μg / μl D-limonene solution prepared above were added to a blank desulfurized rubber plug carrier to prepare the attractant M4.

[0033] 50 μl of the 1 μg / μl verbena alcohol solution, 44 μl of the 1 μg / μl eucalyptol solution, 42 μl of the 1 μg / μl ALPHA-pinene solution and 14 μl of the 1 μg / μl D-limonene solution prepared above were added to a blank devulcanized rubber plug carrier to prepare the attractant M5.

[0034] Lure experiment: From May 29, 2024 to October 30, 2024, the corn fields of the Langfang pilot test base of the Institute of Plant Protection, Chinese Academy of Agricultural Sciences and the Sanya Nanbinhai Reclamation Group of the South China Seed Breeding Research Institute of the Chinese Academy of Agricultural Sciences were used as the experimental sites. Among them, the field lure experiment on fall armyworms was carried out in corn fields with relatively flat terrain, consistent corn growth and management conditions, and large and neat plots.

[0035] Place the prepared lure on the yellow board trap, place the middle of the yellow board trap flush with the top of the crop, and keep the distance between adjacent yellow boards at 20m. Use the n-hexane lure as the control lure. Set three replicates for each treatment (each lure) and arrange them randomly. After setting, investigate the number of fall armyworms attracted every three days. Statistical analysis of the number of fall armyworms caught by different treatments is performed. The results are shown in Figure 1 .

[0036] The results showed that verbenol could not effectively attract the fall armyworm; while eucalyptol, ALPHA-pinene and D-limonene could effectively attract the fall armyworm; further, after combining eucalyptol, ALPHA-pinene and D-limonene, it was found that the combination of the three could significantly improve (P<0.05) the attraction effect on the fall armyworm, indicating that the combination of the three could play a synergistic role; further, after combining verbenol with the three active components, it was found that verbenol could significantly improve (P<0.05) the attraction effect on the fall armyworm at a certain dosage, indicating that verbenol has a synergistic effect on the combination of the three components.

Claims

1. A composition for attracting fall armyworm, comprising active ingredients, wherein the active ingredients include eucalyptol, ALPHA-pinene and D-limonene; Taking the total weight of the active components as 100%, the weight of the eucalyptol accounts for 33% to 50%, the weight of the ALPHA-pinene accounts for 33% to 45%, and the weight of the D-limonene accounts for 5% to 33%.

2. The composition according to claim 1, characterized in that Taking the total weight of the active components as 100%, the weight of the eucalyptol accounts for 44% to 50%, the weight of the ALPHA-pinene accounts for 40% to 45%, and the weight of the D-limonene accounts for 5% to 15%.

3. The composition according to claim 1, characterized in that The composition also includes verbenol.

4. The composition according to claim 3, characterized in that Based on the total weight of the active components as 100%, the weight amount of verbenol is 20% to 40%.

5. Use of the composition according to any one of claims 1 to 4 in attracting fall armyworm.

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