Nanoscale hair growing and nourishing composition and preparation method, pharmaceutical preparation and application thereof

Abstract

The invention provides a nanoscale hair growing and nourishing composition as well as a preparation method, a pharmaceutical preparation and application thereof, and relates to the technical field of traditional Chinese medicine compositions. The nanoscale hair growing and nourishing composition is prepared from the following components in parts by weight: 1 to 20 parts of radix polygoni multiflori, 1 to 15 parts of radix angelicae sinensis, 1 to 15 parts of rhizoma chuanxiong, 1 to 18 parts of radix paeoniae alba, 1 to 12 parts of radix rehmanniae praeparata, 1 to 10 parts of poria cocos, 1 to 10 parts of radix salviae miltiorrhizae and 1 to 15 parts of herba menthae. The preparation method comprises the following steps: grinding and mixing the radix polygoni multiflori, the angelica sinensis, the ligusticum wallichii, the radix paeoniae alba, the prepared rehmannia root, the poria cocos, the salvia miltiorrhiza and the mint according to the prescription dosage. The invention also provides a medicinal preparation containing the nanoscale hair growing and nourishing composition, and application of the composition and the medicinal preparation in preparation of products for preventing alopecia, growing hair and nourishing hair. The technical scheme adopts the traditional Chinese medicine composition, has the functions of promoting hair growth, enhancing hair root health, improving scalp environment and the like, and is safe and effective.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine compositions, and in particular to a nanometer-level hair growth and nourishing composition, a preparation method thereof, a pharmaceutical preparation and application thereof. Background Art

[0002] Hair is part of human tissue and is an organism composed of cells. Hair cells divide from the hair follicles and multiply. New hair will be pushed from the hair follicles to the surface of the scalp skin. It is a normal metabolic symptom to lose less than 30 hairs a day. Each hair follicle will produce new hair within a year. If the hair lacks the necessary active protein (hair cell growth protein), the hair will gradually shrink after each renewal and replacement, gradually changing from thick to thin, from long to short, and then to thin and fine, shrinking until the white hair is invisible to the naked eye, forming the baldness symptoms we see in daily life.

[0003] Hair loss is a common scalp disease that seriously affects the patient's quality of life and mental health. Traditional treatments mainly include topical medications, oral medications and laser therapy, but they have limitations and side effects. Western medicine has a certain effect, but the side effects are large, the effect is not long-lasting and it is easy to relapse, and the overall cure rate is low. Traditional Chinese medicine has the advantages of overall regulation, treating both the symptoms and the root cause, and few side effects, and has been widely accepted and adopted by people. As a traditional treatment method, traditional Chinese medicine is safe, mild, and has comprehensive regulation characteristics, and has certain advantages in the treatment of hair loss.

[0004] Chinese invention CN104940116A discloses an anti-hair loss shampoo, which belongs to the technical field of daily chemical products. It is prepared from the following raw materials in parts by weight: 20-25 parts of Polygonum multiflorum, 10-12 parts of Astragalus, 15-20 parts of Acanthopanax, 15-20 parts of Gastrodia elata, 15-20 parts of yeast, 10-12 parts of black beans, 15-20 parts of Aucklandia lappa, 10-15 parts of ginger, 12-16 parts of Morus alba, 12-15 parts of Lycium barbarum, 0.8-1.0 parts of Camellia oil, 1-1.2 parts of sodium dodecylbenzene sulfonate, 0.5-0.8 parts of coconut oleic acid monoethanolamide and 0.1-0.5 parts of essence. By extracting active ingredients from natural raw materials and various Chinese medicinal herbs, and using them in combination, the prepared shampoo has a good anti-hair loss effect. Although the anti-hair loss effect of this product can reach 70%, it is only from the perspective of preventing hair loss. It does not solve the problem of less hair from the root. It is still necessary to develop products for hair growth and hair care.

[0005] Chinese invention CN110269911A discloses a Chinese medicine formula and preparation method thereof for reducing hair loss and turning white hair into black hair by regulating scalp circulation metabolism and nutrient supply, the formula comprising wolfberry, dodder seed, raspberry, schisandra, plantain seed, ginseng, atractylodes, tuckahoe, liquorice, angelica, chuanxiong, prepared rehmannia root, white peony root, salvia miltiorrhiza, safflower, peach kernel, sappan wood, gastrodia, siler, mulberry leaf, mint, polygonum multiflorum, honeysuckle vine, peony bark, oriental arborvitae, lycium bark and ginseng. The use of the above formula can invigorate blood circulation and dredge meridians, supplement hair root nutrition and regulate blood microcirculation in the head while replenishing essence and blood, dispelling wind, dispersing blood stasis and clearing away heat. The extract prepared by the above formula is directly applied to the hair, or the extract prepared by the formula is used as the main raw material to make shampoo or hair care cream, and administered through the skin, which can not only quickly turn white hair into black hair, but also effectively eliminate the symptoms of hair loss and dry hair; and it has no toxic side effects on the body. However, its formula is too complicated, including 28 kinds of traditional Chinese medicine.

[0006] Due to the accelerated pace of life, increased work pressure, environmental factors and other factors, androgenic alopecia is becoming more and more serious around the world, and people's demand for treatment of hair loss is also growing. Traditional Chinese medicine treatment methods have shown good results in some cases, but there are some challenges in the treatment process, such as drug permeability and effectiveness. However, with the introduction of nanotechnology, it has brought new directions to researchers in this field. In summary, developing a nano-Chinese medicine formula for anti-hair loss with a simple formula, safety and no toxic side effects is the research focus and direction of researchers in this field. Summary of the invention

[0007] In view of the above problems, the present invention provides a nano-scale hair growth and nourishing composition and a preparation method thereof. The formula comprises Polygonum multiflorum, Angelica sinensis, Ligusticum chuanxiong, White Peony Root, Rehmannia glutinosa, Poria cocos, Salvia miltiorrhiza and Menthus sinensis. By optimizing the component ratio and the preparation method, the obtained composition has the functions of promoting hair growth, enhancing the health of hair roots, improving the scalp environment, etc. The composition is safe and effective and is suitable for people of different ages and genders.

[0008] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0009] On the one hand, the present invention provides a nano-scale hair growth and nourishing composition, which comprises the following components in parts by weight: 1-20 parts of Polygonum multiflorum, 1-15 parts of Angelica sinensis, 1-15 parts of Ligusticum chuanxiong, 1-18 parts of White Peony Root, 1-12 parts of Rehmannia glutinosa, 1-10 parts of Poria cocos, 1-10 parts of Salvia miltiorrhiza and 1-15 parts of mint.

[0010] Preferably, the nano-scale hair growth and nourishing composition comprises the following components in parts by weight: 1-15 parts of Polygonum multiflorum, 1-10 parts of Angelica sinensis, 1-12 parts of Ligusticum chuanxiong, 1-15 parts of White Peony Root, 1-10 parts of Rehmannia glutinosa, 1-8 parts of Poria cocos, 1-8 parts of Salvia miltiorrhiza and 1-12 parts of mint.

[0011] Further preferably, the nano-scale hair growth and nourishing composition comprises the following components in parts by weight: 1-10 parts of Polygonum multiflorum, 1-8 parts of Angelica sinensis, 1-10 parts of Ligusticum chuanxiong, 1-12 parts of White Peony Root, 1-8 parts of Rehmannia glutinosa, 1-6 parts of Poria cocos, 1-6 parts of Salvia miltiorrhiza and 1-10 parts of mint.

[0012] More preferably, the nano-scale hair growth and nourishing composition comprises the following components in parts by weight: 5 parts of Polygonum multiflorum, 4 parts of Angelica sinensis, 1 part of Ligusticum chuanxiong, 11 parts of White Peony Root, 8 parts of Rehmannia glutinosa, 4 parts of Poria cocos, 2 parts of Salvia miltiorrhiza and 5 parts of Menthus.

[0013] On the other hand, the present invention provides a method for preparing the above-mentioned nano-scale hair growth and nourishing composition, which comprises grinding and mixing prescribed amounts of Polygonum multiflorum, Angelica sinensis, Ligusticum chuanxiong, White Peony Root, Rehmannia glutinosa, Poria cocos, Salvia miltiorrhiza and mint.

[0014] In yet another aspect, the present invention provides a pharmaceutical preparation comprising the above-mentioned nanoscale hair growth and nourishing composition.

[0015] Preferably, the dosage forms of the pharmaceutical preparation include pills, capsules, granules, oral liquids, powders, tablets, lozenges, pastes and lozenges.

[0016] Preferably, the ointment is an external ointment, and the external ointment further comprises auxiliary materials.

[0017] In another aspect, the present invention provides the use of the above-mentioned nano-scale hair growth and nourishing composition in the preparation of products for preventing hair loss, promoting hair growth and nourishing hair.

[0018] Preferably, the hair loss to be prevented includes hereditary hair loss, stress-induced hair loss or hair loss caused by drugs.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] 1. The technical solution of the present invention highlights the advantages of nano-scale Chinese medicine hair growth formula in terms of efficiency and accuracy compared with traditional methods.

[0021] 2. The technical solution of the present invention is a Chinese medicine combination: a combination of multiple Chinese herbal medicines is used to comprehensively regulate the hair growth environment and promote hair growth.

[0022] 3. Safe and effective: Chinese herbal ingredients are natural and mild, reducing side effects and suitable for long-term use.

[0023] 4. Easy to operate: The preparation is simple and easy to operate, and can be personalized according to the patient's different symptoms and age groups.

[0024] 5. Wide range of applications: Suitable for hair loss caused by various reasons, including hereditary alopecia, androgenic alopecia and stress-induced alopecia, etc. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 The cell viability graph shows that HDP cells were treated with 100, 200, 400 and 800 μM H2O2 for 4 h.

[0026] Figure 2 This is a graph showing the cell proliferation rate of damaged HDP cells in Example 1 after the HDP cells were treated with 800 μM H2O2 for 4 hours.

[0027] Figure 3 These are pictures of the volunteers' hair growth under a digital microscope at 1600 times magnification after using the anti-hair loss and hair growth essence for 0, 35 and 72 days.

[0028] Specific implementation ratio

[0029] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in the field without creative work are within the scope of protection of the present invention. The materials, reagents, etc. used are reagents and materials available from commercial sources unless otherwise specified.

[0030] Source of raw materials: Polygonum multiflorum, Angelica sinensis, Ligusticum chuanxiong, White Peony Root, Rehmannia glutinosa, Poria cocos, Salvia miltiorrhiza, Mint, etc. were purchased from Xi'an Yuankui Biotechnology Co., Ltd.

[0031] Example 1

[0032] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 5 parts of Polygonum multiflorum extract, 4 parts of Angelica sinensis extract, 1 part of Ligusticum chuanxiong extract, 11 parts of White Peony Root extract, 8 parts of Rehmannia glutinosa extract, 4 parts of Poria cocos extract, 2 parts of Salvia miltiorrhiza extract, and 5 parts of peppermint extract.

[0033] Example 2

[0034] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 1 part of Polygonum multiflorum extract, 6 parts of Angelica sinensis extract, 8 parts of Ligusticum chuanxiong extract, 10 parts of White Peony Root extract, 2 parts of Rehmannia glutinosa extract, 2 parts of Poria cocos extract, 2 parts of Salvia miltiorrhiza extract, and 8 parts of peppermint extract.

[0035] Example 3

[0036] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 9 parts of Polygonum multiflorum extract, 2 parts of Angelica sinensis extract, 5 parts of Ligusticum chuanxiong extract, 12 parts of White Peony Root extract, 1 part of Rehmannia glutinosa extract, 4 parts of Poria cocos extract, 2 parts of Salvia miltiorrhiza extract, and 5 parts of peppermint extract.

[0037] Example 4

[0038] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 10 parts of Polygonum multiflorum extract, 8 parts of Angelica sinensis extract, 2 parts of Ligusticum chuanxiong extract, 9 parts of White Peony Root extract, 4 parts of Rehmannia glutinosa extract, 4 parts of Poria cocos extract, 4 parts of Salvia miltiorrhiza extract, and 1 part of peppermint extract.

[0039] Example 5

[0040] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 5 parts of Polygonum multiflorum extract, 1 part of Angelica sinensis extract, 10 parts of Ligusticum chuanxiong extract, 9 parts of White Peony Root extract, 4 parts of Rehmannia glutinosa extract, 2 parts of Poria cocos extract, 1 part of Salvia miltiorrhiza extract, and 8 parts of peppermint extract.

[0041] Example 6

[0042] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 10 parts of Polygonum multiflorum extract, 8 parts of Angelica sinensis extract, 2 parts of Ligusticum chuanxiong extract, 9 parts of White Peony Root extract, 1 part of Rehmannia glutinosa extract, 4 parts of Poria cocos extract, 4 parts of Salvia miltiorrhiza extract, and 2 parts of peppermint extract.

[0043] Example 7

[0044] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 10 parts of Polygonum multiflorum extract, 8 parts of Angelica sinensis extract, 5 parts of Ligusticum chuanxiong extract, 1 part of White Peony Root extract, 4 parts of Rehmannia glutinosa extract, 4 parts of Poria cocos extract, 4 parts of Salvia miltiorrhiza extract, and 2 parts of peppermint extract.

[0045] Example 8

[0046] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 5 parts of Polygonum multiflorum extract, 2 parts of Angelica sinensis extract, 2 parts of Ligusticum chuanxiong extract, 9 parts of Paeonia lactiflora extract, 4 parts of Rehmannia glutinosa extract, 4 parts of Poria cocos extract, 4 parts of Salvia miltiorrhiza extract, and 10 parts of peppermint extract.

[0047] Comparative Example 1 (the difference from Example 1 is that a single component is used)

[0048] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 40 parts of Polygonum multiflorum extract.

[0049] Comparative Example 2 (the difference from Example 1 is that a single component is used)

[0050] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 40 parts of angelica extract.

[0051] Comparative Example 3 (the difference from Example 1 is that a single component is used)

[0052] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 40 parts of chuanxiong extract.

[0053] Comparative Example 4 (the difference from Example 1 is that a single component is used)

[0054] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 40 parts of white peony root.

[0055] Comparative Example 5 (the difference from Example 1 is that a single component is used)

[0056] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 40 parts of Rehmannia glutinosa extract.

[0057] Comparative Example 6 (the difference from Example 1 is that a single component is used)

[0058] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 40 parts of Poria cocos extract.

[0059] Comparative Example 7 (the difference from Example 1 is that a single component is used)

[0060] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 40 parts of salvia miltiorrhiza extract.

[0061] Comparative Example 8 (the difference from Example 1 is that a single component is used)

[0062] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 40 parts of peppermint extract.

[0063] Comparative Example 9 (the difference from Example 1 is that it does not contain Polygonum multiflorum extract)

[0064] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 4 parts of angelica extract, 1 part of chuanxiong extract, 11 parts of white peony extract, 8 parts of rehmannia extract, 4 parts of tuckahoe extract, 2 parts of salvia miltiorrhiza extract and 5 parts of peppermint extract.

[0065] Comparative Example 10 (the difference from Example 1 is that it does not contain Angelica extract)

[0066] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 5 parts of Polygonum multiflorum extract, 1 part of Ligusticum chuanxiong extract, 11 parts of Paeonia lactiflora extract, 8 parts of Rehmannia glutinosa extract, 4 parts of Poria cocos extract, 2 parts of Salvia miltiorrhiza extract, and 5 parts of peppermint extract.

[0067] Comparative Example 11 (the difference from Example 1 is that it does not contain Chuanxiong extract)

[0068] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 5 parts of Polygonum multiflorum extract, 4 parts of Angelica sinensis extract, 11 parts of White Peony Root extract, 8 parts of Rehmannia glutinosa extract, 4 parts of Poria cocos extract, 2 parts of Salvia miltiorrhiza extract, and 5 parts of peppermint extract.

[0069] Comparative Example 12 (the difference from Example 1 is that it does not contain white peony root)

[0070] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 5 parts of Polygonum multiflorum extract, 4 parts of Angelica sinensis extract, 1 part of Ligusticum chuanxiong extract, 8 parts of Rehmannia glutinosa extract, 4 parts of Poria cocos extract, 2 parts of Salvia miltiorrhiza extract and 5 parts of peppermint extract.

[0071] Comparative Example 13 (the difference from Example 1 is that it does not contain Radix Rehmanniae Preparata)

[0072] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 5 parts of Polygonum multiflorum extract, 4 parts of Angelica sinensis extract, 1 part of Ligusticum chuanxiong extract, 11 parts of Paeonia lactiflora extract, 4 parts of Poria cocos extract, 2 parts of Salvia miltiorrhiza extract, and 5 parts of peppermint extract.

[0073] Comparative Example 14 differs from Example 1 in that it does not contain Poria cocos.

[0074] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 5 parts of Polygonum multiflorum extract, 4 parts of Angelica sinensis extract, 1 part of Ligusticum chuanxiong extract, 11 parts of Paeonia lactiflora extract, 8 parts of Rehmannia glutinosa extract, 2 parts of Salvia miltiorrhiza extract, and 5 parts of peppermint extract.

[0075] Comparative Example 15 differs from Example 1 in that it does not contain Salvia miltiorrhiza.

[0076] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 5 parts of Polygonum multiflorum extract, 4 parts of Angelica sinensis extract, 1 part of Ligusticum chuanxiong extract, 11 parts of Paeonia lactiflora extract, 8 parts of Rehmannia glutinosa extract, 4 parts of Poria cocos extract, and 5 parts of peppermint extract.

[0077] Comparative Example 16 differs from Example 1 in that it does not contain mint.

[0078] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 5 parts of Polygonum multiflorum extract, 4 parts of Angelica sinensis extract, 1 part of Ligusticum chuanxiong extract, 11 parts of White Peony Root extract, 8 parts of Rehmannia glutinosa extract, 4 parts of Poria cocos extract, and 2 parts of Salvia miltiorrhiza extract.

[0079] Comparative Example 17 (the difference from Example 1 is that the Polygonum multiflorum extract is replaced with Platycladus orientalis extract having similar efficacy)

[0080] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 5 parts of Platycladus orientalis extract, 4 parts of Angelica sinensis extract, 1 part of Ligusticum chuanxiong extract, 11 parts of Paeonia lactiflora extract, 8 parts of Rehmannia glutinosa extract, 4 parts of Poria cocos extract, 2 parts of Salvia miltiorrhiza extract, and 5 parts of peppermint extract.

[0081] Comparative Example 18 (the difference from Example 1 is that the Angelica extract is replaced with a Gastrodia extract having similar efficacy)

[0082] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 5 parts of Polygonum multiflorum extract, 4 parts of Gastrodia elata extract, 1 part of Ligusticum chuanxiong extract, 11 parts of White Peony Root extract, 8 parts of Rehmannia glutinosa extract, 4 parts of Poria cocos extract, 2 parts of Salvia miltiorrhiza extract, and 5 parts of peppermint extract.

[0083] Comparative Example 19 (the difference from Example 1 is that the Chuanxiong extract is replaced with a Panax notoginseng extract having similar efficacy)

[0084] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 5 parts of Polygonum multiflorum extract, 4 parts of Angelica sinensis extract, 1 part of Panax notoginseng extract, 11 parts of White Peony Root extract, 8 parts of Rehmannia glutinosa extract, 4 parts of Poria cocos extract, 2 parts of Salvia miltiorrhiza extract, and 5 parts of peppermint extract.

[0085] Comparative Example 20 (the difference from Example 1 is that the white peony root extract is replaced with a ginkgo leaf extract having similar efficacy)

[0086] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 5 parts of Polygonum multiflorum extract, 4 parts of Angelica sinensis extract, 1 part of Ligusticum chuanxiong extract, 11 parts of Ginkgo biloba extract, 8 parts of Rehmannia glutinosa extract, 4 parts of Poria cocos extract, 2 parts of Salvia miltiorrhiza extract, and 5 parts of peppermint extract.

[0087] Comparative Example 21 (the difference from Example 1 is that the Radix Rehmanniae Preparata extract is replaced with Rubia cordifolia extract having similar efficacy)

[0088] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 5 parts of Polygonum multiflorum extract, 4 parts of Angelica sinensis extract, 1 part of Ligusticum chuanxiong extract, 11 parts of White Peony Root extract, 8 parts of Rubia cordifolia extract, 4 parts of Poria cocos extract, 2 parts of Salvia miltiorrhiza extract, and 5 parts of peppermint extract.

[0089] Comparative Example 22 (the difference from Example 1 is that the Poria extract is replaced with Sophora flavescens extract having similar efficacy)

[0090] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 5 parts of Polygonum multiflorum extract, 4 parts of Angelica sinensis extract, 1 part of Ligusticum chuanxiong extract, 11 parts of White Peony Root extract, 8 parts of Rehmannia glutinosa extract, 4 parts of Sophora flavescens extract, 2 parts of Salvia miltiorrhiza extract, and 5 parts of peppermint extract.

[0091] Comparative Example 23 (the difference from Example 1 is that the Danshen extract is replaced with a ginseng extract having similar efficacy)

[0092] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 5 parts of Polygonum multiflorum extract, 4 parts of Angelica sinensis extract, 1 part of Ligusticum chuanxiong extract, 11 parts of White Peony Root extract, 8 parts of Rehmannia glutinosa extract, 4 parts of Poria cocos extract, 2 parts of Ginseng extract and 5 parts of Peppermint extract.

[0093] Comparative Example 24 (the difference from Example 1 is that the peppermint extract is replaced by a ginger extract having similar efficacy)

[0094] A nano-scale hair growth and nourishing composition comprises the following raw materials in parts by weight: 5 parts of Polygonum multiflorum extract, 4 parts of Angelica sinensis extract, 1 part of Ligusticum chuanxiong extract, 11 parts of White Peony Root extract, 8 parts of Rehmannia glutinosa extract, 4 parts of Poria cocos extract, 2 parts of Salvia miltiorrhiza extract, and 5 parts of ginger extract.

[0095] Comparative Example 25 (the difference from Example 1 is that the number of components is different, and the amount of each component is the same as that of Example 1 of Chinese invention CN110269911A)

[0096] Test Example 1: DPPH free radical scavenging rate test

[0097] Take the composition prepared in the comparative example of the embodiment, dilute it with deionized water to a concentration of 0.25 mg / mL; add 100 μL of the sample solution to a 96-well plate, and then add 100 μL of 0.2 mmol / LDPPH anhydrous ethanol solution. After shaking evenly, place it in a dark place at 25°C to avoid light for 30 minutes, and then measure the absorbance A1 of the mixed solution at a wavelength of 517 nm; take 100 μL of anhydrous ethanol instead of DPPH anhydrous ethanol solution to perform the same operation; take 100 μL of anhydrous ethanol instead of the sample to perform the same operation; use vitamin C solution as a positive control, set up 3 parallel experiments in each group to calculate the average value, and calculate the free radical scavenging ability of the anti-aging repair composition of different groups according to the DPPH scavenging rate formula.

[0098] DPPH clearance rate = [1-(A1-A2) / A3] × 100%;

[0099] Where:

[0100] A1 is the absorbance value of 100 μL DPPH working solution + 100 μL sample;

[0101] A2 is the absorbance value of 100 μL anhydrous ethanol + 100 μL sample;

[0102] A3 is the absorbance value of 100 μL DPPH working solution + 100 μL anhydrous ethanol.

[0103] The test results can be found in Table 1.

[0104] Table 1. Test results of free radical scavenging ability of Examples and Comparative Examples

[0105]

[0106]

[0107] As can be seen from Table 1, the compositions obtained in Examples 1-8 all have good DPPH free radical scavenging ability, with the highest scavenging rate reaching 98.06%. The free radical scavenging effect of the compositions obtained in Examples 1-8 is much stronger than the effect of using a single component alone in Comparative Examples 1-8, and is also much stronger than the effect of any three components combined in Comparative Examples 9-17. The compositions obtained in Comparative Examples 17-24, due to the use of components with similar efficacy to replace a certain component in the composition of the present invention, have a free radical scavenging effect far lower than that of the compositions of Examples 1-8 of the present invention. Therefore, it is further explained that the components in the nano-scale hair growth and nourishing composition provided by the present invention have a synergistic effect, and the eight components are indispensable. The composition of the present invention has a strong free radical scavenging ability and can effectively improve the hair loss problem caused by free radicals.

[0108] The composition of Comparative Example 25 has a lower DPPH radical scavenging ability than Examples 1-8 due to the different numbers of components, indicating that the specific combination and ratio of the present invention achieves a better DPPH radical scavenging ability.

[0109] Test Example 2: Determination of 5α-reductase activity inhibition

[0110] The invention adopts ultraviolet spectrophotometry to determine the inhibitory effect of the compound Chinese medicine extract on 5α-reductase, measures the absorbance value of the reaction solution at 340nm, and calculates the inhibitory effect of the compound Chinese medicine extract on 5α-reductase according to the change value of the absorbance.

[0111] Take the nano-scale hair growth and nourishing composition prepared in Examples 1-3 and Comparative Examples 1-25, dissolve it ultrasonically with deionized water and 75% ethanol, prepare a sample solution with a Chinese medicine concentration of 1 mg / mL, store it at 4°C, and obtain a recurrent Chinese medicine extract as a sample solution for the determination of the inhibitory activity of the extract. The experiment was repeated three times, with 3 parallel samples each time. Dilute to a concentration of 1.00 mg / mL; add different volumes of 5α-reductase extract (1-4 μL) to 1 mL of the sample solution and the system with a substrate testosterone (T) mass concentration of 3 mg / L and an NADPH concentration of 0.5 mmol / L, respectively, add Buffer to a total volume of 500 μL, react at 37°C for 30 minutes, determine the content of T before and after the reaction, and calculate the conversion amount of T. With the amount of extract added as the horizontal axis and the amount of T conversion as the vertical axis, perform linear regression to calculate the T conversion amount when the amount of extract added is 1 mL. Each group of solutions was placed in a 1.5mL centrifuge tube, vortexed and mixed, kept at 37℃ for 30min, and 0.5mL of methanol was added to terminate the reaction. The BC group was directly used as the test solution, and the other groups were centrifuged at 12000r / min for 10min. The supernatant was taken as the test solution. The content of T was determined by HPLC. Each group was operated in triplicate. The crude drug concentration of 1mg / mL was selected as the sample concentration. The system contained 50μL of the test drug, 300μL of phosphate buffer (20mmol / L, pH=5.5), 50μL of 0.7mmol / LT, 500μL of 900μg / mL 5α-reductase (PBbuffer pH=5.5 dilution), and the reaction started after adding 100μL of 1mg / mL NADPH. The system was incubated at 37°C for 60 min, and the reaction was terminated by adding 2.5 mL of dichloromethane. The mixture was mixed and centrifuged for 15 min (5000 r / min). The organic layer was taken and spin-dried, and methanol was added to dissolve the mixture to 1 mL. The mixture was filtered with a 0.22 μm organic filter membrane for detection of T consumption.

[0112] Finasteride, as a 5α-reductase heterotypic inhibitor, competes with T for 5α-reductase binding sites, thereby irreversibly inhibiting the conversion of T into dihydrotestosterone. In this experiment, finasteride was selected as a positive control, and its effect on 5α-reductase activity was determined using an optimized in vitro enzyme activity assay system. The results showed that finasteride inhibited 5αR2 activity in a dose-dependent manner. When the concentration of finasteride was 1 μmol / L, the inhibition rate could reach 83.21±1.84%. The IC50 of finasteride was determined to be 242±2.5 nmol / L, which is not much different from the 212 nmol / L and 237 nmol / L reported in the literature, and can be used for the screening of 5α-reductase inhibitors.

[0113] Table 2. Reaction system for the study of 5α-reductase inhibitory activity

[0114] category Blank group (uL) Positive group (uL) Test drug group (uL) PB buffer (pH = 5.5) 300 300 300 5α-Reductase Solution 500 500 500 0.7mmol / LT 50 50 50 75% ethanol 50 * * Test drug * * 50 Finasteride * 50 * 1mg / mL NADPH 100 100 100

[0115] Note: “*” indicates that the item is not included.

[0116] Eight active ingredients used in cosmetics were measured using the evaluation system in Table 2. The results are shown in Table 3.

[0117] Table 3. Test results of 5α-reductase activity inhibition in Examples and Comparative Examples

[0118]

[0119]

[0120] As can be seen from Table 3, the compositions obtained in Examples 1-8 all have good 5α-reductase activity inhibition ability, and the highest inhibition rate reaches 68.57%. The 5α-reductase activity inhibition effect of the compositions obtained in Examples 1-8 is stronger than the effect of using a single component alone in Comparative Examples 1-8, and is also much stronger than the effect of any three components combined in Comparative Examples 9-16. The compositions obtained in Comparative Examples 17-24, due to the use of components with similar efficacy to replace a certain component in the composition of the present invention, have a lower 5α-reductase activity inhibition effect than the samples of Examples 1-8 of the present invention. Due to the different number of components, the 5α-reductase activity inhibition ability of the composition of Comparative Example 25 is lower than that of the samples of Examples 1-8. This shows that the plant extract composition of the present invention has a good synergistic effect on the inhibition of 5α-reductase activity. If one of the components is missing, the effect is greatly reduced. The composition of the present invention has a strong 5α-reductase activity inhibition ability and can effectively improve androgenic alopecia caused by excessive 5α-reductase.

[0121] Test Example 3. Determination of the effects of nano-scale Chinese herbal formula on cell proliferation and anti-oxidation

[0122] The composition of Example 1 was subjected to a cell proliferation evaluation experiment.

[0123] Determination of the antioxidant effect of the composition of Example 1 on HDPC cells. HDPC cells were plated into a 96-well plate and divided into a blank group, a damaged group and a sample group, with 3 parallel wells in each group. The blank group was given normal culture medium, and the damaged group was added with H2O2 at the optimal damaging concentration; the sample group was first pretreated with different concentrations of the composition of Example 1 for 24 hours, and then H2O2 at the optimal damaging concentration was added for oxidative damage for 2 hours; then the culture medium was replaced and incubated for another 24 hours, and finally the cell proliferation rate was detected with CCK-8, and the effect of different H2O2 concentrations on the cell proliferation rate was calculated. The calculation formula is as follows:

[0124] Cell proliferation rate = (A1-A2) / (A3-A2) × 100%;

[0125] Where:

[0126] A1: contains absorbance values ​​of cells, CCK-8, samples and H2O2;

[0127] A2: absorbance value containing CCK-8 but excluding cells, samples and H2O2;

[0128] A3: Absorbance value containing cells and CCK-8, but excluding samples and H2O2.

[0129] The results are as follows Figure 1 shown.

[0130] The CCK-8 method was used to detect the activity of the composition of Example 1 on H2O2-induced HDPC cells. The HDPC cells were first treated with 800 μM H2O2 for 4 h, and then pretreated with different concentrations of the composition of Example 1 (0.5 μM, 1 μM, 10 μM and 20 μM) for 24 h. The results are as follows: Figure 2 As shown, compared with the control group, the activity of HDPC cells induced by H2O2 was significantly reduced (p<0.01). Compared with the H2O2 group, as the concentration of the composition of Example 1 increased, the activity of HDPC cells gradually increased, showing a certain dose-dependent relationship (p<0.01). The results show that the composition of Example 1 can enhance the activity of HDPC cells induced by H2O2.

[0131] Performance Test 4: Determination of Inhibition of Microorganisms Causing Scalp Inflammation

[0132] (1) Pick several Staphylococcus aureus colonies and place them in sterilized LB medium. Culture them at 37°C for 18 h and set aside.

[0133] (2) Use a sterile pipette to draw a few milliliters of P. acnes bacterial solution into 10 mL of sterilized FT medium, shake well, seal with sterile vaseline, use the gas-generating capsule method, place in an anaerobic jar and culture at 37°C for 72 hours for later use.

[0134] (3) Use a sterile pipette to draw 0.3-0.5 mL of L.furfur activation and rejuvenation medium, drop it into the ampoule tube, and shake it gently to dissolve the freeze-dried bacteria into a suspended state. Pipette all the bacterial suspension and transplant it into 10 mL of the same culture medium. Culture it at a constant temperature of 30°C for 48 hours. Continue to culture until the third generation and set aside.

[0135] (4) Elute the activated bacterial colonies with sterile saline to prepare a diluted bacterial solution and adjust its concentration to McFarland turbidimetric concentration of 0.5 (1.5×10 8 CFU / mL), and then diluted 100 times with sterile saline to a final concentration of 1×10 5 -10 6CFU / mL is reserved. All the above colony inoculation processes are carried out in a biological safety cabinet.

[0136] The MIC of the nano-scale hair growth and nourishing compositions prepared in Examples 1-3 and Comparative Examples 1-25 against microorganisms was determined by the test tube continuous dilution method. Several test tubes were taken and divided into three groups numbered 1-10 (A1, A2, A3; B1, B2, B3; C1, C2, C3; D1, D2, D3; E1, E2, E3; F1, F2, F3). LB medium was added to the A1, A2, A3 groups, FT medium was added to the B1, B2, B3, M. furfur liquid medium was added to the C1, C2, C3, LB medium was added to the D1, D2, D3, FT medium was added to the E1, E2, E3, and M. furfur liquid medium was added to the F1, F2, F3. Taking the 2-fold dilution method as an example, 1 mL of culture medium was added to each test tube, the tube was sealed with a rubber stopper, and sterilized at 121°C for 30 minutes under high pressure and wet heat. After returning to room temperature, 1 mL of the nano-scale hair growth and nourishing composition prepared in Examples 1-3 and Comparative Examples 1-25 was added to tube 1 of each group, and after being fully mixed, 1 mL was added to tube 2 of each group, and so on, 1 mL of the drug culture medium mixture was taken from tube 9 and discarded, and 1 mL of normal saline was added to tube 10 as a growth control group; all operations were performed in a sterile environment; according to this method, the extract was diluted 5 times (4 mL of culture medium in each tube, 1 mL of compound Chinese medicine extract) and 10 times (9 mL of culture medium in each tube, 1 mL of compound Chinese medicine extract). The dilution degree of the compound Chinese medicine and the corresponding drug content are shown in Tables 4-1, 4-2 and 4-3 below.

[0137]

[0138]

[0139] In each group of test tubes, freshly prepared diluted bacterial solution was added dropwise to each tube until the concentration of diluted bacterial solution in each tube was 1×10 3 -10 4 CFU / mL, shake well. All colony inoculation processes were carried out in a biosafety cabinet. S.aureus was cultured at 37°C for 18 hours; P.acnes was sealed with sterile vaseline, and the gas-producing capsule method was placed in an anaerobic tank for anaerobic culture at 37°C for 72 hours; M.furfur was cultured at 30°C for 48 hours. Observe the phenomenon and record the results. Result judgment: Through naked eye observation, the strain in the maximum drug dilution tube that does not grow is the MIC value of the compound Chinese medicine extract for the strain. When judging, place the test tube in a light-transmitted place and compare it with the growth control tube. The clear and transparent one is sterile growth.

[0140] According to the determination method, the MIC values ​​of the three strains were compared with tube No. 10, and a range of values ​​appeared in the MIC values ​​of each strain. The drug concentrations of the strains that did not grow in the maximum dilution tube of Staphylococcus aureus were 3.75 mg / mL and 6.0 mg / mL, the drug concentrations of the strains that did not grow in the maximum dilution tube of Propionibacterium acnes were 7.5 mg / mL and 6.0 mg / mL, and the drug concentrations of the strains that did not grow in the maximum dilution tube of Malassezia furfur were 7.5 mg / mL and 6.0 mg / mL. The MIC range of the compound Chinese medicine extract is now expressed in the reciprocal of the dilution multiple of the extract.

[0141] Table 4-4. MIC values ​​of compound Chinese medicine extracts and control drugs against three model bacteria

[0142]

[0143] Performance Test 5: Nano-level hair growth and nourishing Chinese medicine tested in animal experiments

[0144] The C57BL / 6 mouse animal model is often used to study the hair growth cycle and evaluate hair growth-promoting activity. The melanocytes of this mouse are only present in the hair follicles, and the production of melanin is closely related to its hair growth cycle. Melanin is synthesized during the growth phase. When the hair follicles are in the regression phase, melanin synthesis decreases, and the skin appears gray. In the resting phase, melanin synthesis stops completely, and the skin returns to pink. Most of the hair follicles of 7-week-old C57BL / 6 mice are in the resting phase. Artificial hair removal can induce their hair to enter the growth phase, and then enter the resting phase after the regression phase. Therefore, the growth cycle can be observed by observing the color changes of the back skin.

[0145] Sixty male C57BL / 6 mice were fed adaptively for one week and then randomly divided into six groups according to their weight using Excel software. Depilatory cream was used to remove hair of approximately 3×4 cm on the back. 2 The skin was pink and undamaged, confirming that the mouse hair follicles were in the resting phase. Animal modeling was performed. Except for the blank group, the mice in each group were subcutaneously injected with testosterone propionate solution (5 mg / (kg·d)) at multiple points on the depilatory area of ​​the back. After 30 minutes of testosterone propionate treatment, 200 μL of the drug solution was applied to the depilatory area on the back of the mice in each group, once a day for 35 consecutive days. The blank group and the model group were given 75% ethanol solution, the minoxidil group was given 5% minoxidil solution, and the low, medium, and high dose groups of component 1 were given Chinese medicine alcohol extract solution (75% ethanol solution, crude drug concentration of 5 mg / mL, 10 mg / mL, 20 mg / mL), and the mice were given doses of 1 mg / (mouse·d), 2 mg / (mouse·d), and 4 mg / (mouse·d), respectively.

[0146] 1. Observation of hair growth

[0147] The skin and hair growth of the depilatory area of ​​each group of mice were observed every day, and the time when the skin color of the depilatory area of ​​each mouse changed from pink to black and the time when hair began to grow were recorded to evaluate the effect of the drug on the hair growth cycle of mice. Each group was photographed and the hair growth score was recorded on days 0, 7, 14, 21, 28, and 35 of drug administration. The scoring criteria were:

[0148] 0 points: no growth, the skin in the hair removal area is pink;

[0149] 1 point: the skin in the hair removal area is gray (less than 20% growth);

[0150] 2 points: the skin in the hair removal area is black (greater than 20% and less than 40%);

[0151] 3 points: The skin in the hair removal area is black with a little hair growth (greater than 40% and less than 60%);

[0152] 4 points: the skin in the hair removal area is black and some hair grows (60-80% growth);

[0153] 5 points: The hair in the depilated area is almost completely grown (80% to 100% growth).

[0154] 2. Determination of hair quality

[0155] After blood collection, mice were killed by dislocating the neck, and a 3×4 cm hair removal area on the back of each mouse was shaved with an electric shaver. 2 The gross weight of the area is weighed on an analytical balance and the average value is calculated.

[0156] 3. Tissue Sectioning, Fixation and Staining

[0157] After shaving the hair, the skin was sampled parallel to the spine at the hair removal site, and the skin was trimmed into 1 cm wide strips. After being fixed in 4% paraformaldehyde for 24-36 hours, the paraformaldehyde was washed away, the blocks were embedded in paraffin, and the sections were sliced. Finally, after HE staining, the number and morphological differences of hair follicles were observed and compared under an optical microscope. The hair growth and hair weight of each group of mice during the experiment are shown in Table 5. Compared with the blank group, the skin discoloration time and hair growth time of the model group mice were significantly prolonged (P<0.05), and the hair weight was significantly reduced on the 35th day (P<0.05). The results showed that subcutaneous injection of testosterone propionate can significantly inhibit the growth of hair in C57BL / 6 mice. Compared with the model group, the skin discoloration time, hair growth time and hair quality of the minoxidil group and the Ligustrum lucidum alcohol extract groups were significantly different (P<0.05), indicating that minoxidil and component 1 alcohol extract can alleviate the inhibitory effect of testosterone propionate on hair growth in C57BL / 6 mice to a certain extent.

[0158] Table 5. Hair growth of mice in each group n=10)

[0159]

[0160] Note: Compared with the blank group, *P<0.05; compared with the model group, #P<0.05.

[0161] Performance Test 6: Determination of the Human Efficacy Test of Nano-Level Chinese Herbal Formula for Hair Growth and Nourishing

[0162] The present invention, together with Zhongshan Perfect Testing Technology Co., Ltd., verified the efficacy of the anti-hair loss and hair growth essence on human body, and the test method and results are as follows.

[0163] 1. Volunteer selection criteria

[0164] 1. Healthy Chinese men aged 18-60 years old;

[0165] 2. No serious chronic wasting diseases (asthma, diabetes, etc.);

[0166] 3. The volunteer has hair loss, seborrheic alopecia on the top of the head;

[0167] 4. Volunteers have not used any form of hair growth agent or any treatment for hair growth in the past month;

[0168] 5. Volunteers can complete the experiment strictly according to the requirements of the research plan.

[0169] II. Exclusion criteria

[0170] 1. The volunteer suffers from a disease that may affect the trial results;

[0171] 2. Those who have received antihistamines or anti-allergy treatment in the past week;

[0172] 3. The tested area suffers from acute inflammation or other diseases;

[0173] 4. Participating in drug clinical trials or participating in other human safety and efficacy trials of cosmetics during the trial period;

[0174] 5. Have you used similar products for hair loss prevention and hair growth in the past month?

[0175] 3. Exit Criteria During the trial, if a volunteer has adverse reactions, is lost without reason, or violates the research protocol (such as using cosmetics or drugs that affect the research results) or other special reasons, and after professional evaluation and confirmation, if the volunteer is no longer suitable to continue the trial, the volunteer will be asked to withdraw. After screening by technical personnel, 30 volunteers who met the requirements were finally selected to complete the trial.

[0176] method:

[0177] Test material: Nano-scale hair growth and nourishing compositions prepared in Examples 1-3 and Comparative Examples 1-25

[0178] Test environment: temperature 22±1℃, relative humidity 50±3% Test part: hair loss area & whole head.

[0179] Evaluation method:

[0180] All volunteers applied the anti-hair loss and hair growth essence to the designated areas of the scalp twice a day. The subjects were followed up on the 0th, 35th and 72nd day and the results were recorded.

[0181]

[0182] After using the anti-hair loss essence for 72 days, the volunteers showed improvement in all test indicators, such as decreased sebum secretion, reduced hair loss quantity and area, increased number of hair follicles, etc. The improvement of these indicators indicates that the compound Chinese medicine extract in the anti-hair loss essence can inhibit the activity of 5α-reductase in hair follicles, inhibit the activity of microorganisms such as Malassezia furfur that cause scalp inflammation, promote human scalp microcirculation, provide nutrition to hair follicles, delay hair follicle atrophy, prevent hair loss and promote hair growth, as shown in the results of in vitro efficacy experiments.

[0183] The above results show that the nano-scale hair growth and nourishing Chinese medicine formula of the present invention contains a hair growth and nourishing composition, which has multiple anti-hair loss and hair growth effects, has a synergistic effect on the treatment of oxidative free radical hair loss, effectively inhibits the activity of 5α-reductase, and has a relieving effect on androgenic alopecia, hair loss caused by bacteria and fungi, and maintains a good scalp microecology. The natural composition with hair growth and nourishing effects of the present invention is mild and non-irritating, the scalp is refreshed, and it is highly safe for long-term use. In addition, the preparation method of the hair growth and nourishing cosmetics of the present invention is simple and convenient, which is conducive to large-scale production and application.

[0184] The above description is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several modifications or equivalent substitutions can be made to the technical solution without departing from the principle of the present invention. These modifications or equivalent substitutions should also be regarded as the protection scope of the present invention.

Claims

1. A nanoscale hair growth and nourishing composition, characterized in that: According to weight fractions, it comprises the following components: 1-20 parts of Polygonum multiflorum, 1-15 parts of Angelica sinensis, 1-15 parts of Ligusticum chuanxiong, 1-18 parts of White Peony Root, 1-12 parts of Rehmannia glutinosa, 1-10 parts of Poria cocos, 1-10 parts of Salvia miltiorrhiza and 1-15 parts of mint.

2. The nanoscale hair growth and nourishing composition according to claim 1, characterized in that: The nano-scale hair growth and nourishing composition comprises the following components in parts by weight: 1-15 parts of Polygonum multiflorum, 1-10 parts of Angelica sinensis, 1-12 parts of Ligusticum chuanxiong, 1-15 parts of White Peony Root, 1-10 parts of Rehmannia glutinosa, 1-8 parts of Poria cocos, 1-8 parts of Salvia miltiorrhiza and 1-12 parts of mint.

3. The nanoscale hair growth and nourishing composition according to claim 2, characterized in that: The nano-scale hair growth and nourishing composition comprises the following components in parts by weight: 1-10 parts of Polygonum multiflorum, 1-8 parts of Angelica sinensis, 1-10 parts of Ligusticum chuanxiong, 1-12 parts of White Peony Root, 1-8 parts of Rehmannia glutinosa, 1-6 parts of Poria cocos, 1-6 parts of Salvia miltiorrhiza and 1-10 parts of mint.

4. The nanoscale hair growth and nourishing composition according to claim 1, characterized in that: The nano-scale hair growth and nourishing composition comprises the following components by weight: 5 parts of Polygonum multiflorum, 4 parts of Angelica sinensis, 1 part of Ligusticum chuanxiong, 11 parts of White Peony Root, 8 parts of Rehmannia glutinosa, 4 parts of Poria cocos, 2 parts of Salvia miltiorrhiza and 5 parts of Menthus officinalis.

5. The method for preparing the nano-scale hair growth and nourishing composition according to any one of claims 1 to 4, characterized in that: Grind and mix the prescribed amounts of Polygonum multiflorum, Chinese angelica, Ligusticum chuanxiong, White Peony Root, Rehmannia glutinosa, Poria cocos, Salvia miltiorrhiza and mint.

6. A pharmaceutical preparation, characterized in that A nanoscale hair growth and nourishing composition comprising any one of claims 1 to 4.

7. The pharmaceutical preparation according to claim 6, characterized in that The dosage forms of the pharmaceutical preparation include pills, capsules, granules, oral liquids, powders, tablets, lozenges, pastes and lozenges.

8. The pharmaceutical preparation according to claim 7, characterized in that The ointment is an external ointment, and the external ointment also contains auxiliary materials.

9. Use of the nanoscale hair growth and nourishing composition according to any one of claims 1 to 4 or the pharmaceutical preparation according to any one of claims 6 to 8 in the preparation of products for preventing hair loss, promoting hair growth and nourishing hair.

10. The use according to claim 9, characterized in that: The hair loss that is prevented from hair loss includes hereditary hair loss, stress-induced hair loss or hair loss caused by drugs.

Images