Application of longdomesticated wild rice OlARF9 gene in improving rice resistance to hypoxia stress

By overexpressing the OlARF9 gene of wild rice with long male florets in rice, the problems of germination and seedling development of rice under hypoxia stress were solved, the hypoxia stress resistance of rice was improved, and the improvement of rice stress resistance and molecular breeding were promoted.

CN119193611BActive Publication Date: 2026-07-24YUNNAN UNIV
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Patent Information

Application Number
CN202411474807.9
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-10-22
Publication Date
2026-07-24
Estimated Expiration
2044-10-22

AI Technical Summary

Technical Problem

The existing technology has not found any application of the OlARF9 protein or its encoding gene in improving the plant's tolerance to hypoxia stress, which affects the rapid and uniform germination and normal seedling development of rice under flooded conditions.

Method used

By constructing a recombinant plant expression vector containing the OlARF9 gene of wild rice and transforming it into rice cells, transgenic rice overexpressing the OlARF9 gene was cultivated, enhancing its resistance to hypoxia stress.

Benefits of technology

It improved the coleoptile length and seedling survival rate of transgenic rice under hypoxic conditions, enhanced the rice's resistance to hypoxia stress, and promoted the improvement of rice's stress resistance and the process of molecular breeding.

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Abstract

The application discloses application of long-male wild rice OlARF9 gene in improving rice resistance to low-oxygen stress and belongs to the field of bioengineering. The long-male wild rice OlARF9 gene is overexpressed in rice by using overexpression technology, and it is found according to phenotype changes of transgenic plants that overexpression of the OlARF9 gene in rice can significantly improve the ability of the rice to resist low-oxygen stress, and therefore, the long-male wild rice OlARF9 gene can be applied to improving the non-biological stress resistance of the rice, including overexpression of the long-male wild rice OlARF9 gene in the rice to obtain a transgenic plant, and the obtained transgenic plant has obviously enhanced resistance to low-oxygen stress. The application has application prospects in aspects of improving and enhancing the stress resistance of the rice and accelerating the process of stress resistance molecular breeding.
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