A reagent for detecting HIV antibodies in urine and a preparation method thereof

By using colloidal gold-antigen conjugates with truncated gp41-gp36 fusion antigen in urine HIV antibody detection, the problem of poor sensitivity and inability to detect HIV-1 and HIV-2 antibodies at the same time was solved, and high sensitivity and specificity were achieved.

CN119199108BActive Publication Date: 2025-03-18BEIJING KEYUE ZHONGKAI BIOTECHNOLOGY CO LTD
4 Cites 0 Cited by

Patent Information

Application Number
CN202411736924.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-11-29
Publication Date
2025-03-18
Estimated Expiration
2044-11-29

AI Technical Summary

Technical Problem

The existing urine HIV antibody detection methods are poorly sensitive and cannot detect HIV-1 and HIV-2 antibodies at the same time. The sensitivity of urine antibodies after ART treatment is reduced, resulting in inaccurate detection results.

Method used

A reagent for detecting urine HIV antibodies is used, including PVC base plate, sample pad, colloidal gold pad, nitrocellulose membrane and water absorbing pad. The colloidal gold pad contains colloidal gold-antigen conjugate, and the antigen is a truncated gp41-gp36 fusion antigen, which can detect HIV-1 and HIV-2 antibodies simultaneously.

Benefits of technology

It improves the sensitivity and specificity of HIV antibodies in urine, and can achieve test results within 15 minutes. It is suitable for different populations, including patients after ART treatment.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The present invention relates to the field of medical detection, and in particular to a reagent for detecting urine HIV antibodies and a preparation method thereof. The reagent is a test strip, which includes a PVC base plate and a sample pad, a colloidal gold pad, a nitrocellulose membrane and a water-absorbing pad that are sequentially overlapped and pasted on the PVC base plate in the direction of chromatography, and the colloidal gold pad includes a colloidal gold antigen conjugate, and the antigen in the colloidal gold antigen conjugate is gp41-1-gp36, and the gp41-1-gp36 is a truncated gp41-gp36 fusion antigen; the nitrocellulose membrane is provided with a detection line T coated with mouse anti-human IgG antibody and a quality control line C coated with sheep anti-mouse IgG antibody. The present invention improves the detection sensitivity by optimizing the combination of reactant components and antigen combination, and can realize the simultaneous detection of urine HIV-1 and HIV-2 antibodies within 15 minutes, improves the detection efficiency, and expands the scope of application.
Need to check novelty before this filing date? Find Prior Art