Composition for improving anoxia tolerance and preparation method and application thereof

Abstract

The invention discloses a composition for improving anoxia tolerance and a preparation method and application thereof, and belongs to the technical field of health-care products, and the composition comprises the following raw materials in parts by weight: 3-9 parts of turnip, 3-10 parts of sea-buckthorn and 9-30 parts of codonopsis pilosula. The composition can improve the hypoxia tolerance of animal bodies and improve the symptoms such as dizziness, headache, palpitation, shortness of breath and the like caused by hypoxia.

Description

Technical Field

[0001] The present invention belongs to the technical field of health care products, and in particular relates to a composition for improving hypoxia tolerance, and a preparation method and application thereof. Background Art

[0002] Hypoxia refers to a pathological process in which tissue metabolism, function and morphological structure undergo abnormal changes due to insufficient oxygen supply or oxygen use disorder. When the body's oxygen supply is less than the oxygen demand, cells will adjust their metabolic levels to adapt to tissue needs. This process affects life processes such as cell survival, proliferation, movement, metabolism, pH regulation, extracellular matrix function, inflammatory cell recruitment and angiogenesis.

[0003] The impact of hypoxia on the body depends on the degree, speed, duration and functional metabolic state of hypoxia. Generally, mild hypoxia mainly causes compensatory reactions of the body, such as changes in blood circulation, increased breathing and accelerated red blood cell production. In some severe hypoxia, when the animal body has no time to compensate or the compensation is incomplete, obvious metabolic dysfunction will occur, and even local cell, tissue and organ necrosis will occur. In particular, hypoxia of important vital organs such as the brain and heart often directly leads to animal death. Therefore, hypoxia plays a core role in the pathogenesis and pathophysiology of cardiovascular diseases, nervous system diseases, respiratory diseases, etc. In addition, the plateau hypoxic environment can cause damage to important organs such as the brain, lungs, heart, and gastrointestinal tract in the human body. Among them, the brain tissue with vigorous metabolism, large blood flow and high oxygen consumption has very poor tolerance to hypoxia and is extremely susceptible to hypoxia damage. When the body enters a rapid hypoxic environment, brain damage manifestations such as acute mountain sickness, plateau headache, and plateau cerebral edema will occur.

[0004] Most of the existing products for improving hypoxia tolerance use Rhodiola rosea as raw material. Although they have certain effects, the output of Rhodiola rosea raw materials is limited, so the output of the final product cannot be guaranteed. Therefore, it will be of great value to develop new products that improve hypoxia tolerance. Summary of the invention

[0005] In view of the above-mentioned deficiencies in the prior art, the present invention provides a composition for improving hypoxia tolerance and a preparation method and application thereof. The composition can improve the hypoxia tolerance of animals and improve symptoms such as dizziness, headache, palpitations and shortness of breath caused by hypoxia.

[0006] To achieve the above purpose, the technical solution adopted by the present invention to solve the technical problem is:

[0007] A composition for improving hypoxia tolerance comprises the following raw materials in parts by weight: 3-9 parts of turnip, 3-10 parts of seabuckthorn and 9-30 parts of codonopsis pilosula.

[0008] Furthermore, 6-12 portions of Ophiopogon japonicus are also included.

[0009] Furthermore, 1-3 parts of taurine are included.

[0010] Furthermore, it also includes 0.01-0.2 parts of pyrroloquinoline quinone.

[0011] Furthermore, the following raw materials are included in parts by weight: 9 parts of turnip, 9 parts of seabuckthorn, 15 parts of codonopsis pilosula, and 9 parts of ophiopogon japonicus.

[0012] The method for preparing the above-mentioned composition for improving hypoxia tolerance comprises the following steps:

[0013] (1) soaking the Chinese medicinal materials except pyrroloquinoline quinone and taurine in water, then decocting them twice, combining the two decoctions, concentrating them, and obtaining a water extract;

[0014] (2) Add pyrroloquinoline quinone and / or taurine to the aqueous extract to obtain the product.

[0015] Furthermore, in step (1), the amount of water added during soaking is 9-11 times the weight of the medicinal materials, and the soaking time is 2-4 hours.

[0016] Furthermore, in step (1), each decoction time is 0.5-1.5h.

[0017] Furthermore, the relative density of the concentrated product in step (2) is 2.0-2.5.

[0018] The use of the above-mentioned composition for improving hypoxia tolerance in the preparation of functional foods or health products for improving hypoxia tolerance.

[0019] The beneficial effects produced by the present invention are:

[0020] 1. The composition of the present invention can significantly prolong the hypoxia tolerance time at normal pressure and effectively improve the hypoxia tolerance of the human body.

[0021] 2. The composition of the present invention has simple ingredients and the production process is simple and easy to operate. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a statistical chart of the weight changes of mice;

[0023] Figure 2 This is a statistical chart of the survival time of mice under hypoxia. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical scheme and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention, that is, the embodiments described are only part of the embodiments of the present invention, rather than all of the embodiments.

[0025] Therefore, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present invention.

[0026] It should be noted that relational terms such as "first" and "second" are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprises" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, an element defined by the sentence "comprises a ..." does not exclude the existence of other identical elements in the process, method, article or device including the element.

[0027] The features and performance of the present invention are further described in detail below in conjunction with the embodiments and drawings.

[0028] Example 1

[0029] A composition for improving hypoxia tolerance comprises the following raw materials in parts by weight: 9 parts of turnip, 9 parts of seabuckthorn and 15 parts of codonopsis pilosula.

[0030] The method for preparing the above-mentioned composition for improving hypoxia tolerance comprises the following steps:

[0031] Soak turnip, seabuckthorn and codonopsis in 10 times the amount of water for 3 hours, then boil twice, each time for 1 hour, combine the two decoctions, filter and concentrate to obtain a concentrated solution with a relative density of 2.0.

[0032] Example 2

[0033] A composition for improving hypoxia tolerance comprises the following raw materials in parts by weight: 9 parts of turnip, 9 parts of seabuckthorn, 15 parts of codonopsis pilosula and 9 parts of ophiopogon japonicus.

[0034] The method for preparing the above-mentioned composition for improving hypoxia tolerance comprises the following steps:

[0035] Add 10 times the amount of water to soak turnip, seabuckthorn, Codonopsis pilosula and Ophiopogon japonicus for 3 hours, then decoct twice, each time for 1 hour, combine the two decoctions, filter and concentrate to obtain a concentrate with a relative density of 2.5.

[0036] Example 3

[0037] A composition for improving hypoxia tolerance comprises the following raw materials in parts by weight: 9 parts of turnip, 9 parts of seabuckthorn, 15 parts of codonopsis pilosula, 9 parts of ophiopogon japonicus, 1 part of taurine and 0.15 parts of pyrroloquinoline quinone (PQQ).

[0038] The method for preparing the above-mentioned composition for improving hypoxia tolerance comprises the following steps:

[0039] Add 10 times the amount of water to soak turnip, seabuckthorn, codonopsis and ophiopogon for 3 hours, then decoct twice, each time for 1 hour, combine the two decoctions, filter and concentrate to obtain a concentrate with a relative density of 2.2, add taurine and PQQ to the concentrate to obtain.

[0040] Example 4

[0041] A composition for improving hypoxia tolerance comprises the following raw materials in parts by weight: 9 parts of turnip, 9 parts of seabuckthorn, 15 parts of codonopsis pilosula and 1 part of taurine.

[0042] The method for preparing the above-mentioned composition for improving hypoxia tolerance comprises the following steps:

[0043] Soak turnip, seabuckthorn and codonopsis in 10 times the amount of water for 3 hours, then boil twice, each time for 1 hour, combine the two decoctions, filter and concentrate to obtain a concentrate with a relative density of 2.0, add taurine to the concentrate to obtain.

[0044] Example 5

[0045] A composition for improving hypoxia tolerance comprises the following raw materials in parts by weight: 9 parts of turnip, 9 parts of seabuckthorn, 15 parts of codonopsis pilosula and 0.15 parts of PQQ.

[0046] The method for preparing the above-mentioned composition for improving hypoxia tolerance comprises the following steps:

[0047] Soak turnip, seabuckthorn and codonopsis in 10 times the amount of water for 3 hours, then decoct twice, each time for 1 hour, combine the two decoctions, filter and concentrate to obtain a concentrate with a relative density of 2.4, add PQQ to the concentrate to obtain.

[0048] Example 6

[0049] A composition for improving hypoxia tolerance comprises the following raw materials in parts by weight: 9 parts of turnip, 9 parts of seabuckthorn, 15 parts of codonopsis, 1 part of taurine and 0.15 parts of PQQ.

[0050] The method for preparing the above-mentioned composition for improving hypoxia tolerance comprises the following steps:

[0051] Soak turnip, seabuckthorn and codonopsis in 10 times the amount of water for 3 hours, then boil twice, each time for 1 hour, combine the two decoctions, filter and concentrate to obtain a concentrate with a relative density of 2.2, add taurine and PQQ to the concentrate to obtain.

[0052] Example 7

[0053] A composition for improving hypoxia tolerance comprises the following raw materials in parts by weight: 3 parts of turnip, 3 parts of seabuckthorn, 30 parts of codonopsis pilosula, 12 parts of ophiopogon japonicus, 2 parts of taurine and 0.2 parts of PQQ.

[0054] The method for preparing the above-mentioned composition for improving hypoxia tolerance comprises the following steps:

[0055] Add 10 times the amount of water to soak turnip, seabuckthorn, codonopsis and ophiopogon for 3 hours, then decoct twice, each time for 1 hour, combine the two decoctions, filter and concentrate to obtain a concentrate with a relative density of 2.0, add taurine and PQQ to the concentrate to obtain.

[0056] Example 8

[0057] A composition for improving hypoxia tolerance comprises the following raw materials in parts by weight: 6 parts of turnip, 6 parts of seabuckthorn, 20 parts of codonopsis pilosula, 6 parts of ophiopogon japonicus, 1 part of taurine and 0.1 part of PQQ.

[0058] The method for preparing the above-mentioned composition for improving hypoxia tolerance comprises the following steps:

[0059] Add 10 times the amount of water to soak turnip, seabuckthorn, codonopsis and ophiopogon for 3 hours, then decoct twice, each time for 1 hour, combine the two decoctions, filter and concentrate to obtain a concentrate with a relative density of 2.0, add taurine and PQQ to the concentrate to obtain.

[0060] Comparative Example 1

[0061] It is Nordicon capsule (the main active ingredient is Rhodiola rosea, 0.28g per capsule). The capsule is prepared in the form of aqueous solution with a concentration of 0.28g / kg, which is 10 times the recommended dose for humans.

[0062] Test example

[0063] Mouse hypoxia tolerance experiment

[0064] 1. Animal Experiment Grouping and Methods

[0065] A total of 96 clean-grade SPF KM male mice, 3-4 weeks old, weighing 18-20g, were selected for the experiment and randomly divided into 9 groups, namely, blank group, model group, positive control group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, Example 6 group, 12 mice in each group. Before the formal experiment, the mice were kept in the animal laboratory for 7 days to adapt to the environment. The breeding environment is: temperature 20-25 ° C, humidity 40-50%, artificial lighting 12h / d, good ventilation, mice free to eat and drink water, and the mouse cage bedding is completely replaced every 2 days to keep the environment dry and clean.

[0066] After the animals were grouped, the blank group and the model group were gavaged with pure water (0.1 mL / 10 g), and the positive control group and the formula group were gavaged with the formula drug suspension (0.1 mL / 10 g) for 1 week. The following pharmacodynamics verification was performed according to the above grouping method.

[0067]

[0068]

[0069] 2. Sodium nitrite poisoning survival experiment

[0070] The drug was administered orally for 30 consecutive days. One hour after the last administration, all groups except the blank group were intraperitoneally injected with 200 mg / kg NaNO2 sodium nitrite solution (the volume of the injected solution was approximately 0.1 mL per 10 g of mouse body weight). The survival time of the mice was immediately recorded.

[0071] 3. Data Analysis

[0072] The data were statistically analyzed using the SPSS data analysis function. The quantitative data were expressed as mean ± standard deviation. One-way analysis of variance was used, and P < 0.05 was considered statistically significant. The embodiment and comparative example were analyzed for significance with the model group, respectively. * indicates P < 0.05, which is significantly correlated, and ** indicates P < 0.01, which is extremely significantly correlated.

[0073] 4. Results

[0074] 4.1 Weekly weight changes of mice

[0075] The weekly body weights of mice are shown in the table below.

[0076] Table 2 Weekly weight of mice

[0077]

[0078]

[0079] As shown in Table 1, the weekly weight changes of mice, the body weights of KM mice in all groups continued to increase and did not fluctuate much within 6 weeks, maintaining a stable growth state.

[0080] 4.2 Survival time of sodium nitrite poisoning

[0081] The survival time of mice induced by sodium nitrite poisoning and hypoxia (min, mean ± standard deviation, n = 12) is shown in the following table.

[0082] Table 3 Survival timetable of sodium nitrite poisoning and hypoxia in mice

[0083] Group Survival time Model 12.97±1.21 Positive Control 16.07±1.49** Formula 1 15.94±1.06* Formula 2 16.77±1.25*** Formula 3 15.65±1.85* Formula 4 16.66±3.31** Formula 5 15.59±2.20* Formula 6 14.75±1.52*

[0084] As shown in Table 3, compared with the survival time of mice in the model group, the survival time of mice in groups 1-6 was longer than that in the model group, indicating that the formulation ratio provided by the present invention can significantly prolong the survival time of mice poisoned by sodium nitrite, that is, improve the hypoxia tolerance of mice, especially the improvement effect of groups 2 and 4 is more obvious.

[0085] The analysis found that turnip is rich in natural antioxidants such as vitamin C and sulfide, and turnip has the effects of clearing away heat, promoting dampness and detoxifying; seabuckthorn contains active substances such as flavonoids, vitamin C, and carotenoids, and seabuckthorn has the effects of removing phlegm, removing stagnation, and dispersing blood stasis; Codonopsis contains effective ingredients such as polysaccharides, steroidal saponins, and high isoflavones, and Codonopsis has the effects of tonifying the middle and replenishing qi, nourishing yin and promoting fluid, moistening the lungs and refreshing. When turnip, seabuckthorn and Codonopsis are used together, the active ingredients in the medicine can remove free radicals in the body, so that the production and removal of free radicals in a low-oxygen environment maintain a dynamic balance, and reduce the damage of free radicals to the human body; and from the perspective of traditional Chinese medicine, the above-mentioned medicinal materials can be used together to tonify the middle and replenish qi, promote blood circulation and remove blood stasis, remove phlegm and disperse blood stasis, improve the patient's physical fitness, and then improve resistance and hypoxia tolerance; Ophiopogon contains polysaccharides, saponins, brass, biological The present invention contains a variety of effective ingredients, such as alkali, sterols and amino acids. Ophiopogon japonicus has the effects of nourishing yin, promoting body fluid, moistening the lungs and clearing the heart, which can assist in improving lung function, promote the operation of qi in the body, and thus improve the body's tolerance to hypoxia; modern research shows that taurine has the effects of inhibiting platelet aggregation, lowering blood lipids, and protecting myocardial cells. Adding taurine can assist in improving cardiovascular function; PQQ, as a oxidoreductase cofactor, participates in the redox reaction in the body, can remove free radicals in the body, and reduce the damage of free radicals to the human body; in the present invention, on the basis of turnip, seabuckthorn and Codonopsis pilosula, Ophiopogon japonicus, taurine and PQQ are selectively added, which can improve the body's tolerance to hypoxia to a certain extent, but experiments have found that the more ingredients added, the better. It is speculated that the more ingredients added, the more intolerance the body has, which in turn affects the body's tolerance to hypoxia.

[0086] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A composition for improving hypoxia tolerance, characterized in that: The invention comprises the following raw materials in parts by weight: 3-9 parts of turnip, 3-10 parts of seabuckthorn and 9-30 parts of codonopsis pilosula.

2. The composition for improving hypoxia tolerance according to claim 1, characterized in that: Also includes 6-12 portions of Ophiopogon japonicus.

3. The composition for improving hypoxia tolerance according to claim 1 or 2, characterized in that: Also includes 1-3 portions of taurine.

4. The composition for improving hypoxia tolerance according to claim 3, characterized in that: It also contains 0.01-0.2 parts of pyrroloquinoline quinone.

5. The composition for improving hypoxia tolerance according to claim 4, characterized in that: The invention comprises the following raw materials in parts by weight: 9 parts of turnip, 9 parts of seabuckthorn, 15 parts of codonopsis pilosula and 9 parts of ophiopogon japonicus.

6. The method for preparing the composition for improving hypoxia tolerance according to any one of claims 1 to 5, characterized in that: The following steps are involved: (1) soaking the Chinese medicinal materials except pyrroloquinoline quinone and taurine in water, then decocting them twice, combining the two decoctions, concentrating them, and obtaining a water extract; (2) Add pyrroloquinoline quinone and / or taurine to the aqueous extract to obtain the product.

7. The method for preparing the composition for improving hypoxia tolerance according to claim 6, characterized in that: During the soaking in step (1), the amount of water added is 9-11 times the weight of the medicinal materials, and the soaking time is 2-4 hours.

8. The method for preparing the composition for improving hypoxia tolerance according to claim 6, characterized in that: The decoction time in step (1) is 0.5-1.5h each time.

9. The method for preparing a composition for improving hypoxia tolerance according to claim 6, characterized in that: The relative density of the concentrated product in step (2) is 2.0-2.

5.

10. Use of the composition for improving hypoxia tolerance according to any one of claims 1 to 5 in the preparation of functional foods or health products for improving hypoxia tolerance.

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