A strain of thin-skinned casein and its cultivation method and application

By using the V11 strain of *Cephalotaxus fortunei* and its cultivation method, employing composite substrates such as corn cobs and specific steps, the problem of low cultivation efficiency of *Cephalotaxus fortunei* was solved, achieving high yield and high biological efficiency, enriching the germplasm resource bank, and providing a basis for the widespread cultivation and resource development of *Cephalotaxus fortunei*.

CN119220413BActive Publication Date: 2025-10-28黑龙江省农业科学院牡丹江分院
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Patent Information

Application Number
CN202411417782.9
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-10-11
Publication Date
2025-10-28
Estimated Expiration
2044-10-11

AI Technical Summary

Technical Problem

The domestication and cultivation efficiency of existing thin-skinned casei is low, and the biological efficiency is also low. There is an urgent need for high-quality strains and efficient cultivation methods to expand the germplasm resource bank.

Method used

We provide strain V11 of the thin-skinned casei and its cultivation method, which uses a composite substrate formula of corn cob, sawdust, wheat bran, gypsum and lime, combined with specific cultivation and fruiting management steps, including sterilization, inoculation, mycelium cultivation, post-ripening culture, bud induction and fruiting, and fruiting management, to achieve high yield and high biological efficiency.

Benefits of technology

This study achieved high yield, high biological efficiency, and rich nutritional content of the fruiting bodies of *Cephalotaxus fortunei*, providing a scientific basis for the widespread cultivation of *Cephalotaxus fortunei* and enhancing the development and utilization of macrofungi germplasm resources.

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Abstract

The present invention provides a Tyromyces chioneus strain, which is Tyromyces chioneus V11 and has a deposit number of GDMCC No. 65077. The present invention also provides a cultivation method for the Tyromyces chioneus, comprising the steps of pre-treatment of culture medium, bagging and sterilization, inoculation and cultivation, post-ripening culture, bud-inducing and fruiting, and fruiting management, to obtain Tyromyces chioneus fruiting bodies, followed by harvesting and post-harvest management, and secondary fruiting. The *Cephalotaxus fortunei* strain of this invention can produce two flushes of mushrooms, with a fruiting body yield of 196.80±5.21 g / bag, a biological efficiency of 35.14±0.93%, a total flavonoid content of 708 mg / 100g, a crude protein content of 16.30 g / 100g, a dietary fiber content of 18.82 g / 100g, and a calcium content of 554 mg / kg. The strain exhibits a lignin degradation rate of 33.93%, a cellulose degradation rate of 37.72%, and a hemicellulose degradation rate of 28.96%.
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