Traditional Chinese medicine charcoal medicine for promoting estrogen secretion
By preparing dried ginger charcoal and regulating hormone levels, the ovulation disorder caused by polycystic ovarian syndrome was solved, which significantly improved ovarian and uterine functions, promoted follicle generation, and achieved regulation of estrogen levels and improvement of reproductive organ structure. .
Patent Information
- Application Number
- CN202310815629.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-07-05
- Publication Date
- 2025-01-07
AI Technical Summary
The prior art has not effectively solved the problem of ovulation disorder caused by polycystic ovarian syndrome, especially methods to improve menstrual conditions by affecting ovarian and uterine function have not been fully explored.
By preparing dried ginger charcoal and firing it in a muffle furnace through a crucible sealed in tin foil, drugs that promote estrogen secretion are prepared for affecting ovarian and uterine functions. Dry ginger charcoal solution is used for short-term or long-term administration. , regulates hormone levels and genital structure in mice.
Dried ginger charcoal significantly affected the estrogen, FSH and LH levels of mice, improved the function of the ovaries and uterus, increased the thickness and uterine index, promoted the generation of mature follicles, and improved the pathological status of the reproductive organs.
Smart Images
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Abstract
Description
Technical Field
[0001] The invention relates to the field of medicine, and in particular to a new application of dried ginger charcoal in the field of medicine. Background Art
[0002] Ovulatory disorders are an important cause of female infertility, often associated with polycystic ovary syndrome, and the exact mechanism is still unclear. Recent studies have proposed several theories to explain this phenomenon, including inhibition of ovarian aromatase activity, insufficient follicle-stimulating hormone signaling, and insulin resistance. Sex hormones are essential for regulating female reproduction by influencing the development of the ovaries and uterus and regulating the menstrual cycle. To a certain extent, estrogen is secreted by granulosa cells, and its function is to promote folliculogenesis, thereby increasing the expression of gonadotropin receptors, promoting endometrial regeneration and epithelial cell proliferation, and maintaining secondary sexual characteristics.
[0003] Dried ginger is the dried rhizome of Zingiber oj-jicinale Rosc., a plant of the ginger family. It has the effects of warming the middle and dispelling cold, restoring yang and unblocking meridians, and warming the lungs and transforming phlegm. In traditional Chinese medicine, dried ginger charcoal is often used as a hemostatic agent, especially for the treatment of menstrual cramps or gastrointestinal bleeding that occurs during the menstrual cycle in women. The purpose of this study was to investigate whether dried ginger charcoal can improve menstrual conditions and affect ovarian and uterine function by affecting female hormones. Summary of the invention
[0004] In view of this, the object of the present invention is to provide a safe and inexpensive Chinese herbal charcoal medicine having the effect of promoting estrogen secretion, so as to be used in combination or alone in clinical practice.
[0005] Specifically, the present invention is achieved by using dried ginger charcoal in the preparation of a drug for promoting estrogen secretion, and the preparation of dried ginger charcoal comprises the following steps:
[0006] Put the dried ginger raw medicinal slices into a crucible, wrap and seal the crucible mouth with tin foil, cover the crucible with a lid, put it into a muffle furnace and set a certain temperature for firing.
[0007] The invention provides a new use of dried ginger charcoal. BRIEF DESCRIPTION OF THE DRAWINGS
[0008] Figure 1 :Effects of dried ginger charcoal on serum E2( Figure 1 A) FSH ( Figure 1 B) and LH( Figure 1 C) Effect of level. In the figure, control, H, M, and L represent the control group, high-dose, medium-dose, and low-dose groups of dried ginger charcoal, respectively.
[0009] Figure 2 :Estradiol levels in serum of female mice after administration of exogenous estradiol by dried ginger charcoal Figure 2A), FSH( Figure 2 B) and LH( Figure 2 C) level. Control group (control), model group (Model), and high (H), medium (M), and low (L) dose groups of dried ginger charcoal. In the figure, control, Model, H, M, and L represent the control group, model group, and high, medium, and low dose groups of dried ginger charcoal, respectively.
[0010] Figure 3 :Uterine index( Figure 3 A), ovarian thickness ( Figure 3 B), appearance of uterus ( Figure 3 C) uterine tissue pathological section ( Figure 3 C) Ovarian tissue pathological section ( Figure 3 C). In the figure, control, Model, H, M, and L represent the control group, model group, and high-, medium-, and low-dose groups of dried ginger charcoal, respectively. DETAILED DESCRIPTION
[0011] The present invention is further explained below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. After reading the present invention, various equivalent modifications of the present invention by those skilled in the art all fall within the scope defined by the claims attached to this application.
[0012] Embodiment 1: A method for preparing dried ginger charcoal, comprising the following steps:
[0013] The dried ginger medicinal material was placed in a sealed crucible and calcined in a muffle furnace for 1 hour (calcination temperature 375° C.). After the muffle furnace was naturally cooled to room temperature, the carbonized dried ginger was taken out.
[0014] Example 2: Effect of short-term intervention with dried ginger charcoal on hormone levels in normal female mice
[0015] 2.1 Sample preparation
[0016] Preparation of dried ginger charcoal solution: add water to the dried ginger charcoal and boil it. Concentrate the filtrate to make a 1 g / mL (calculated based on the amount of charcoal powder) solution and store it in a 4°C refrigerator for later use.
[0017] 2.2 Animal grouping and drug administration
[0018] Twenty SPF female Kunming mice were randomly divided into a control group and a high-, medium-, and low-dose (0.4 g / kg, 0.2 g / kg, and 0.1 g / kg) group of dried ginger charcoal, with a total of 4 groups, 5 mice in each group. After the adaptive feeding, the high-, medium-, and low-dose groups of dried ginger charcoal were continuously gavaged for 7 days, and the control group was given an equal volume of deionized water.
[0019] 2.3 Detection of hormone levels in serum
[0020] Blood was collected from the inner canthus of the mice, and the blood was allowed to stand at room temperature for 4 hours, centrifuged at 3000 r / min for 10 minutes, and the upper serum was collected. ELISA kits were used to detect the levels of estradiol (E2), follicle stimulating hormone (FSH) and luteinizing hormone (LH) in the serum.
[0021] Results Figure 1 A, Dry ginger charcoal has a reducing effect on the E2 level of normal female mice, and there are significant differences between the medium and low dose groups and the blank group.
[0022] Serum FSH concentration Figure 1 As shown in B. Compared with the control group, the serum FSH levels in the medium and low dose groups of dried ginger charcoal were significantly increased.
[0023] In addition, Figure 1 C shows that after 7 days of intragastric administration, the serum LH levels of mice showed an upward trend, and the low-dose group was significantly higher than the control group.
[0024] Example 3: Effects of long-term intervention with dried ginger charcoal on estrogen
[0025] 3.1 Animal grouping, modeling and drug administration
[0026] SPF female Kunming mice were randomly divided into 5 groups: control group, model group, and high, medium, and low dose groups of dried ginger charcoal. The high, medium, and low dose groups were continuously gavaged for 21 days, and the model and control groups were given the same volume of deionized water. On the 22nd day, the high, medium, and low dose groups of dried ginger charcoal and the model group were subcutaneously injected with 0.6 mg / kg estradiol solution (dissolved in soybean oil for injection), and the control group was injected with soybean oil.
[0027] 3.2 Detection of hormones in serum
[0028] One hour after the administration, blood was collected from the eyeballs of the mice. After standing, the blood was centrifuged at 3000 rpm for 10 minutes to obtain serum. Elisa kits were used to detect the levels of estradiol (E2), follicle stimulating hormone (FSH) and luteinizing hormone (LH) in mice.
[0029] Figure 2 A shows the serum E2 level. The serum estradiol levels of the model group and the high-, medium-, and low-dose groups of dried ginger charcoal were significantly higher than those of the control group. The serum estradiol levels of mice in the medium- and low-dose groups of dried ginger charcoal were significantly higher than those in the model group.
[0030] Figure 2 B shows the serum FSH concentration. Compared with the control group, the FSH concentration in the model group was significantly lower. The high, medium and low dose groups of dried ginger charcoal were significantly higher than that in the model group.
[0031] Serum LH concentration Figure 2C shows that there was no statistical difference in serum LH levels among mice in the model and high-, medium-, and low-dose groups of dried ginger charcoal compared with the control group.
[0032] 3.3 Effects on the uterus and ovaries
[0033] The mouse uterus and ovaries were removed, weighed, and fixed with 10% paraformaldehyde.
[0034] Uterine index Figure 3 As shown in A, compared with the control group, the ovarian index of the low-dose ginger charcoal group increased significantly, while there was no significant change in the other groups.
[0035] As described in 3B, the ovarian thickness of the high, medium and low dose groups of dried ginger charcoal was significantly increased compared with the control group.
[0036] The appearance of the uterus Figure 3 As shown in C, the uterus of the mice in the control group continued to stretch and was yellow, while the uterus of the mice in the model group was short, thick, and white. Compared with the model group, the uterus of the mice in each group of Ganjiangcharo was longer in shape.
[0037] 3.4 Observation of pathological sections of uterine and ovarian tissues
[0038] The right ovary of the mouse was dehydrated using a gradient method and then fixed in a 10% paraformaldehyde solution for 24 hours. Hematoxylin and eosin (H&E) was used to stain 4 μm serial sections and then examined under a photoelectric microscope.
[0039] Endometrial thickness and uterine tissue pathological sections Figure 3 As shown in C, the endometrial thickness of mice in the model group was significantly greater than that in the control group. The endometrial thickness of mice in each group decreased significantly after receiving dried ginger charcoal and was comparable to that of the control animals.
[0040] Ovarian tissue pathology sections Figure 3 As shown in C, the ovaries of female mice in the control group were well developed, with radial follicle crowns and evenly distributed granulosa cells. Exogenous estradiol increased the number of primary follicles in mice, while reducing the number of secondary follicles and mature follicles. Compared with the model group, the high, medium, and low dose groups of dried ginger charcoal showed an increase in the number of mature follicles.
Claims
1. Use of dried ginger charcoal in the preparation of drugs for promoting estrogen secretion.
2. The use according to claim 1, characterized in that Dried ginger charcoal promotes the release of estrogen and follicle-stimulating hormone.
3. The use according to claim 1, characterized in that The dried ginger charcoal and pharmaceutically acceptable excipients are prepared into clinical preparations; the clinical preparations include but are not limited to injections, oral liquid preparations, hard capsules, soft capsules, tablets, suppositories, water pills, honey pills, concentrated pills, ointments, powders, emulsions, pastes, and smears.
4. The use according to claim 1, characterized in that Use with other drugs or alone.