Composition for resisting fatigue and improving exercise tolerance, beverage and application

Abstract

The invention provides a composition for resisting fatigue and improving exercise tolerance, a beverage and application, and belongs to the technical field of functional food, health care products or exercise nutrition. The composition for resisting fatigue and improving exercise tolerance is prepared from the following raw materials in parts by weight: 0.2 to 6 parts of radix ginseng extract, 0.2 to 8.4 parts of radix astragali seu hedysari extract, 0.15 to 2 parts of fructus lycii extract and 0.3 to 3.4 parts of aronia melanocarpa extract. The ginseng extract, the radix astragali extract, the fructus lycii extract and the aronia melanocarpa extract are mutually compatible and synergistically take effect, and the effects of resisting fatigue, improving hypoxia tolerance and improving exercise tolerance can be achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of functional foods, health products or sports nutrition, and in particular relates to a composition, a beverage and an application thereof for resisting fatigue and improving sports endurance. Background Art

[0002] When people engage in labor and sports, they consume a lot of energy, causing fatigue. At present, anti-fatigue foods or health products can help users relieve fatigue, but lack the effect of improving hypoxia tolerance and improving sports endurance. Summary of the invention

[0003] The purpose of the present invention is to provide a composition for resisting fatigue and improving sports endurance, and a preparation method and application thereof. The composition of the present invention can not only resist fatigue, but also improve hypoxia resistance and sports endurance.

[0004] The invention provides a composition for resisting fatigue and improving sports endurance, comprising the following raw materials in parts by weight: 0.2-6 parts of ginseng extract, 0.2-8.4 parts of astragalus extract, 0.15-2 parts of wolfberry extract and 0.3-3.4 parts of aronia suffruticosa extract.

[0005] Preferably, the following raw materials are included in parts by weight: 0.67-3.4 parts of ginseng extract, 0.67-3.4 parts of astragalus extract, 0.5-1.4 parts of wolfberry extract and 1-2.3 parts of aronia suffruticosa extract.

[0006] Preferably, the ginseng extract includes ginseng water extract; the astragalus extract includes astragalus water extract; the wolfberry extract includes wolfberry water extract; and the aronia nigra extract includes aronia nigra water extract.

[0007] Preferably, the saponin content in the ginseng water extract is ≥2%; the wolfberry extract includes wolfberry powder; the polysaccharide content in the wolfberry powder is ≥50%; and the Aronia nigromaculata water extract includes Aronia nigromaculata fruit powder.

[0008] The present invention also provides a beverage, comprising the composition described in the above scheme and water.

[0009] Preferably, the mass content of the composition in the beverage is ≥ 2%.

[0010] Preferably, the mass content of the composition in the beverage is 2.84% to 17.2%.

[0011] The present invention also provides the use of the composition or beverage described in the above scheme in preparing food or health products having one or more of the following functions 1) to 3): 1) anti-fatigue; 2) improving sports endurance; 3) improving hypoxia tolerance.

[0012] Preferably, the food or health product is suitable for people who participate in endurance sports or people who do long-term physical labor.

[0013] The present invention also provides a method for improving exercise endurance and hypoxia tolerance, comprising the following steps:

[0014] The composition or beverage described in the above scheme is taken orally before exercising.

[0015] The present invention provides a composition for resisting fatigue and improving sports endurance, comprising the following raw materials in parts by weight: 0.2-6 parts of ginseng extract, 0.2-8.4 parts of astragalus extract, 0.15-2 parts of wolfberry extract and 0.3-3.4 parts of aronia suffruticosa extract. The present invention can resist fatigue, improve hypoxia tolerance and improve sports endurance by combining the four components of ginseng extract, astragalus extract, wolfberry extract and aronia suffruticosa extract with each other to work synergistically. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0017] Figure 1 This is the result of the mouse endurance running test;

[0018] Figure 2 This is the result of the mouse fatigue rotarod test;

[0019] Figure 3 This is the result of the weighted swimming test for mice;

[0020] Figure 4 This is the result of the mouse hypoxia tolerance test;

[0021] Figure 5 This is a graph showing the results of measuring liver ATP content in mice after exercise.

[0022] Figure 6 This is the result of the measurement of SOD content in mouse blood;

[0023] Figure 7 This is the result of measuring the MDA content in mouse blood;

[0024] Figure 8 This is the result of the determination of SOD content in mouse liver;

[0025] Fig. 9 This is the result of measuring the MDA content in mouse liver. DETAILED DESCRIPTION

[0026] The invention provides a composition for resisting fatigue and improving sports endurance, comprising the following raw materials in parts by weight: 0.2-6 parts of ginseng extract, 0.2-8.4 parts of astragalus extract, 0.15-2 parts of wolfberry extract and 0.3-3.4 parts of aronia suffruticosa extract.

[0027] In the present invention, the composition is preferably composed of the following raw materials in parts by weight: 0.2-6 parts of ginseng extract, 0.2-8.4 parts of astragalus extract, 0.15-2 parts of wolfberry extract and 0.3-3.4 parts of aronia suffruticosa extract.

[0028] In the present invention, the composition includes 0.2 to 6 parts of ginseng extract, preferably 0.67 to 3.4 parts, and more preferably 1 to 2.4 parts by weight. In the present invention, the ginseng extract preferably includes ginseng water extract; the content of saponin in the ginseng water extract is preferably ≥ 2%. In the specific implementation of the present invention, the saponin content of the ginseng extract is 2%, which is purchased from Weihai Songling Nuo Kejia Chinese Medicine Pieces Co., Ltd.;

[0029] In the present invention, the composition comprises 0.2 to 8.4 parts of astragalus extract, preferably 0.67 to 3.4 parts, and more preferably 1 part by weight. In the present invention, the astragalus extract preferably comprises an astragalus water extract. In the specific implementation of the present invention, the astragalus extract is a 10-fold water-extracted astragalus extract purchased from Shaanxi Hongda Plant Chemical Co., Ltd.

[0030] In the present invention, the composition includes 0.15 to 2 parts of wolfberry extract, preferably 0.4 to 1.4 parts, and more preferably 0.5 parts by weight. In the present invention, the wolfberry extract preferably includes wolfberry water extract; the wolfberry water extract includes wolfberry powder; the content of polysaccharides in the wolfberry powder is ≥ 50%; in the specific implementation of the present invention, the wolfberry powder has a polysaccharide content of 50%, which is purchased from Hunan Jiecui Biotechnology Co., Ltd.

[0031] In the present invention, the composition comprises 0.3 to 3.4 parts of Aronia nigra extract, preferably 0.7 to 2.3 parts, and more preferably 1 part by weight. In the present invention, the Aronia nigra extract comprises Aronia nigra water extract; the Aronia nigra water extract preferably comprises Aronia nigra fruit powder. In the specific implementation of the present invention, the Aronia nigra fruit powder is purchased from Liaoning Lingxiushan Mineral Beverage Co., Ltd.

[0032] The present invention also provides a beverage, comprising the composition described in any one of the above schemes and water.

[0033] The beverage of the present invention is convenient to consume, has good storage stability, and has a simple preparation method, and can meet the requirements of actual production and use.

[0034] In the present invention, the mass content of the composition in the beverage is preferably ≥ 2%.

[0035] In a specific implementation of the present invention, the mass content of the composition in the beverage is 2.84%, 9.5% or 17.2%.

[0036] In the present invention, the method for preparing the beverage comprises the following steps: dissolving the composition in water to obtain the beverage.

[0037] The present invention also provides the use of the composition or beverage described in the above scheme in the preparation of food or health products having one or more of the following functions 1) to 3): 1) anti-fatigue; 2) improving exercise endurance; 3) improving hypoxia tolerance.

[0038] In the present invention, the applicable population of the food or health care product preferably includes people who engage in endurance sports or people who engage in long-term physical labor.

[0039] The present invention also provides a method for improving exercise endurance and hypoxia tolerance, comprising the following steps: orally taking the composition or beverage described in the above scheme before exercising.

[0040] In the present invention, the oral dosage of the beverage is preferably 30 mL / d for a person weighing 60 kg.

[0041] In order to further illustrate the present invention, the anti-fatigue and sports endurance improving composition provided by the present invention and its preparation method and application are described in detail below in conjunction with the accompanying drawings and examples, but they should not be understood as limiting the scope of protection of the present invention.

[0042] In the following examples and comparative examples, the sources of raw materials are as follows:

[0043] Ginseng extract, saponin content 2%, was purchased from Weihai Songling Nuo Kejia Chinese Medicine Pieces Co., Ltd.; Astragalus extract (10 times water extraction) was purchased from Shaanxi Hongda Plant Chemical Co., Ltd.; Lycium barbarum powder, polysaccharide content 50%, was purchased from Hunan Jiecui Biotechnology Co., Ltd.; Aronia nigra fruit powder was purchased from Liaoning Lingxiushan Mineral Beverage Co., Ltd. Blueberry fruit powder was purchased from Tuofeng Powder Technology (Kunshan) Co., Ltd.

[0044] The formulations of Examples 1 to 3 and Comparative Examples 1 to 6 are shown in Table 1 below.

[0045] Table 1 Formulas of beverages of Examples 1 to 3 and Comparative Examples 1 to 6

[0046]

[0047] Example 1

[0048] According to mass percentage, the formula consists of: 0.67% ginseng extract, 0.67% astragalus extract, 0.5% wolfberry powder, 1% aronia nigra fruit powder and 97.16% water.

[0049] Preparation method: Ginseng extract, astragalus extract, wolfberry powder and aronia nigra fruit powder are dissolved in water to obtain the product.

[0050] Example 2

[0051] According to mass percentage, the formula consists of: 2.4% ginseng extract, 3.4% astragalus extract, 1.4% wolfberry powder, 2.3% aronia solani fruit powder and 90.5% water.

[0052] The preparation method is the same as that of Example 1.

[0053] Example 3

[0054] According to mass percentage, the formula consists of: 3.4% ginseng extract, 8.4% astragalus extract, 2% wolfberry powder, 3.4% aronia solani fruit powder and 82.8% water.

[0055] The preparation method is the same as that of Example 1.

[0056] Comparative Example 1

[0057] According to mass percentage, the formula consists of: 0.67% astragalus extract, 0.5% wolfberry powder, 1% aronia solani fruit powder and 97.83% water.

[0058] The preparation method is the same as that of Example 1.

[0059] Comparative Example 2

[0060] According to mass percentage, the formula consists of: 0.67% ginseng extract, 0.5% wolfberry powder, 1% aronia nigra fruit powder and 97.83% water.

[0061] The preparation method is the same as that of Example 1.

[0062] Comparative Example 3

[0063] According to mass percentage, the formula consists of: 0.67% ginseng extract, 0.67% astragalus extract, 1% aronia nigra fruit powder and 97.66% water.

[0064] The preparation method is the same as that of Example 1.

[0065] Comparative Example 4

[0066] According to mass percentage, the formula consists of: 0.67% ginseng extract, 0.67% astragalus extract, 0.5% wolfberry powder and 98.16% water.

[0067] The preparation method is the same as that of Example 1.

[0068] Comparative Example 5

[0069] According to mass percentage, the formula consists of: 0.3% ginseng extract, 0.3% astragalus extract, 0.4% wolfberry powder, 0.5% aronia nigra fruit powder and 98.5% water.

[0070] The preparation method is the same as that of Example 1.

[0071] Comparative Example 6

[0072] According to mass percentage, the formula consists of: 0.67% ginseng extract, 0.67% astragalus extract, 0.5% wolfberry powder, 1% blueberry powder and 97.16% water.

[0073] The preparation method is the same as that of Example 1.

[0074] Test Example 1

[0075] 50 healthy male Kunming mice were adapted for 7 days before the experiment and randomly divided into 10 groups, 5 mice in each group, namely: control group, Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, Comparative Example 5 group and Comparative Example 6 group. The drugs were administered by gavage according to the groups.

[0076] Groups of Examples 1 to 3 and Groups of Comparative Examples 1 to 6: The beverages prepared in Examples 1 to 3 and Comparative Examples 1 to 6 were administered to mice by gavage, and the dosage was 30 mL / d for a 60 kg body weight human, and the corresponding proportional intake for a mouse (weighing about 20 g) was 0.1 mL / d.

[0077] Control group: 0.1mL / 20g normal saline was administered by intragastric gavage;

[0078] The drug was administered by intragastric gavage for 28 days continuously, and the mice were free to eat and drink water during the administration period.

[0079] New experimental mice were used in each group of experiments as follows.

[0080] 1. Anti-fatigue test

[0081] 1.1 Endurance running test

[0082] 30 minutes after the last oral administration, the mice in Example 1 to Comparative Example 6 and the control group were placed on an animal treadmill (FT-200, Chengdu Taimeng Software Co., Ltd.) to keep the muscles in a static tension state. The speed of the rotating rod was adjusted to 20 r / min, and the time when each group of mice fell from the glass rod due to muscle fatigue was recorded. The suitability training was performed for 3 days before the experiment, 3 times a day, and the time the mice stayed on the rotating rod was recorded. The experiment was repeated 3 times. For results, see Figure 1 .

[0083] 1.2 Fatigue Rotating Rod

[0084] 30 minutes after the last oral administration, the mice in Example 1 to Comparative Example 6 and the control group were placed on a fatigue rotarod instrument (ZB-200, Chengdu Taimeng Software Co., Ltd.) to keep the muscles in a state of static tension. The speed of the rotarod was adjusted to 20 r / min, and the time when each group of mice fell from the glass rod due to muscle fatigue was recorded. The suitability training was performed for 3 days before the experiment, 3 times a day, and the time the mice stayed on the rotarod was recorded. The experiment was repeated 3 times. For results, see Figure 2 .

[0085] 1.3 Weighted swimming test

[0086] 30 minutes after the last oral administration, mice with doses from Example 1 to Comparative Example 6 and the control group were placed in a weighted swimming test device, with a weight of 10% of body weight tied to the tail of each group of mice. The mice were placed in a bucket-type swimming pool with a water temperature of (22±1)°C and a water depth of d=30 cm, and the continuous swimming time and exhaustion time of each group of mice were measured. Figure 3 .

[0087] 2. Hypoxia resistance test

[0088] 2.1 Normal pressure hypoxia experiment

[0089] 60 minutes after the last oral administration of the drug to the mice, each group of mice was placed in a 250 ml wide-mouth bottle containing 20 g of soda lime. The bottle mouth was smeared with vaseline and covered tightly to keep it airtight. The time from the time the mouse was placed in the bottle to the time the mouse died was observed and recorded, using respiratory arrest as an indicator.

[0090] 3. Tissue collection and determination of related indicators

[0091] 30 minutes after the last oral administration, the mice were placed in a plastic bucket with a water depth of 30 cm, a diameter of 15 cm, and a water temperature of (22±1)°C. After swimming for 20 minutes, blood was collected from the eye sockets, serum was collected, and the mice were killed to collect the liver tissues of the mice. The livers were homogenized, and the contents of ATP, SOD, and MDA in the liver and the contents of SOD and MDA in the blood were determined.

[0092] 4. Statistics

[0093] GraphPad Prism 8 statistical software was used to perform t-test for comparison of two sample means in each group of data. P<0.05 indicates that there is a statistical difference between the groups.

[0094] 5. Test results and analysis

[0095] 5.1 Anti-fatigue

[0096] Depend on Figure 1 , Figure 2 , Figure 3 It can be obtained that the anti-fatigue and exercise endurance improving composition of the present invention has a significant anti-fatigue effect. In the endurance running test, the running time of mice in Example 1, Example 2 and Example 3 was increased by 104%, 173% and 123% respectively compared with the control group (P < 0.01); in the fatigue rotating rod test of mice, the time of mice in Example 1, Example 2 and Example 3 was increased by 61%, 80% and 75% respectively compared with the control group (P < 0.01); in the weighted swimming test of mice, the time of mice in Example 1, Example 2 and Example 3 was increased by 110%, 195% and 134% respectively compared with the control group (P < 0.01).

[0097] 5.2 Hypoxia tolerance test

[0098] Depend on Figure 4 It can be seen that the anti-fatigue and exercise endurance improving composition of the present invention significantly increases the survival time under hypoxia. In the hypoxia tolerance test, the survival time of mice in Example 1, Example 2 and Example 3 was increased by 31%, 62% and 46% respectively compared with the control group (P < 0.05).

[0099] 5.3 Tissue collection and determination of related indicators

[0100] 5.3.1 Detection of ATP content in mouse liver

[0101] ATP is the most direct energy source for the body, responsible for storing and transferring chemical energy, and providing energy for the body. 30 minutes after intragastric administration, the mice swam for 20 minutes. Compared with the control group, the ATP content in the liver of the mice in Example 1, Example 2 and Example 3 increased by 47%, 63% and 54% respectively (P < 0.05). Figure 5 The results suggest that the fatigue resistance and hypoxia tolerance of the embodiments may be partly based on their regulatory effects on ATP in the liver.

[0102] 5.3.2 Contents of SOD and MDA in mouse plasma and liver

[0103] After strenuous exercise, a large amount of free radicals will be produced in the body, lipid peroxidation will occur, the permeability of the membrane will be changed, and fatigue will occur. Antioxidant enzymes can inhibit lipid oxidation of oxygen free radicals, and have extensive cell protection and anti-fatigue effects. SOD is a free radical scavenger, which is widely present in various tissues and cells of organisms, removes oxygen free radicals, and eliminates harmful substances produced in the metabolism of organisms. MDA is the final product of membrane lipid peroxidation, which will aggravate membrane damage and has cytotoxicity. The degree of damage to the membrane system can be indirectly determined by measuring the MDA content in cells and tissues.

[0104] Depend on Figure 6 It can be seen that the examples significantly increased the SOD content in the blood of mice after exercise. Compared with the control group, the SOD content in the blood of mice in Examples 1, 2 and 3 increased by 16%, 30% and 18% respectively (P < 0.05).

[0105] Depend on Figure 7 It can be seen that the examples significantly reduce the MDA content in the blood of mice after exercise. Compared with the control group, the MDA content in the blood of mice in Examples 1, 2 and 3 is reduced by 29%, 54% and 39% respectively (P < 0.05).

[0106] Depend on Figure 8 It can be seen that the examples significantly increased the SOD content in the liver of mice after exercise. Compared with the control group, the SOD content in the liver of mice in Examples 1, 2 and 3 increased by 55%, 114% and 66% respectively (P < 0.05).

[0107] Depend on Fig. 9 It can be seen that the examples significantly reduce the MDA content in the liver of mice after exercise. Compared with the control group, the MDA content in the liver of mice in Examples 1, 2 and 3 was reduced by 37%, 47% and 43% respectively (P<0.05).

[0108] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A composition for resisting fatigue and improving sports endurance, characterized in that: The invention comprises the following raw materials in parts by weight: 0.2-6 parts of ginseng extract, 0.2-8.4 parts of astragalus extract, 0.15-2 parts of wolfberry extract and 0.3-3.4 parts of aronia suffruticosa extract.

2. The composition according to claim 1, characterized in that The invention comprises the following raw materials in parts by weight: 0.67 to 3.4 parts of ginseng extract, 0.67 to 3.4 parts of astragalus extract, 0.5 to 1.4 parts of wolfberry extract and 1 to 2.3 parts of aronia suffruticosa extract.

3. The composition according to claim 1 or 2, characterized in that The ginseng extract includes ginseng water extract; the astragalus extract includes astragalus water extract; the wolfberry extract includes wolfberry water extract; and the aronia nigra extract includes aronia nigra water extract.

4. The composition according to claim 3, characterized in that The content of saponin in the ginseng water extract is ≥4%; the wolfberry extract includes wolfberry powder; the content of polysaccharide in the wolfberry powder is ≥50%; the black fruit glandular costa water extract includes black fruit glandular costa fruit powder.

5. A beverage, characterized in that The composition comprises the composition according to any one of claims 1 to 4 and water.

6. The beverage according to claim 5, characterized in that The mass content of the composition in the beverage is ≥2%.

7. The beverage according to claim 6, characterized in that The mass content of the composition in the beverage is 2.84% to 17.2%.

8. Use of the composition according to any one of claims 1 to 4 or the beverage according to any one of claims 5 to 7 in the preparation of food or health products having one or more of the following functions 1) to 3): 1) anti-fatigue; 2) improving exercise endurance; 3) improving tolerance to hypoxia.

9. The use according to claim 8, characterized in that: The food or health product is suitable for people who participate in endurance sports or people who do long-term physical labor.

10. A method for improving exercise endurance and hypoxia tolerance, characterized in that: The following steps are involved: The composition according to any one of claims 1 to 4 or the beverage according to any one of claims 5 to 7 is orally taken before exercising.

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