Three-chrysanthemum anti-allergy repairing agent as well as preparation method and application thereof

Abstract

The invention discloses a three-chrysanthemum anti-allergy repairing agent as well as a preparation method and application thereof. The three-chrysanthemum anti-allergy repairing agent is prepared from a chrysanthemum parthenium (CHRYSANTHUM) extract, a cornflower (CENTAUREA CYANUS) extract and a helichrysum fortunei (HELICHRYSUM STOECHAS) extract, and the three-chrysanthemum anti-allergy repairing agent is prepared from the following raw materials: the chrysanthemum parthenium extract, the cornflower CYANUS extract and the helichrysum fortunei extract, the cornflower CYANUS extract and the helichrysum fortunei STOECHAS extract. An improved extraction process is adopted, the obtained optimized three-chrysanthemum anti-allergy repairing agent fully extracts oil-soluble components and water-soluble components of parthenocissus parthenocissus, cornflower and French helichrysum, a liposome preparation form is adopted, skin barriers can be permeated more easily, and the anti-allergy repairing effect is improved and optimized more comprehensively and deeply.

Description

Technical Field

[0001] The invention relates to the field of cosmetics, and in particular to a chrysanthemum anti-allergic repairing agent and a preparation method and application thereof. Background Art

[0002] In recent years, due to the deterioration of the ecological environment and the abuse of various industrial additives, the number of people who feel that their skin is sensitive has increased year by year, and sensitive skin has become a major factor affecting human health. Sensitive skin is a special skin condition, which means that when the skin is stimulated by external stimuli, it is easy to have objective symptoms such as erythema, papules, and capillary dilation accompanied by subjective symptoms such as itching, stinging, burning, and tightness.

[0003] Most of the research on the anti-allergic effect of modern medicinal plants is based on the theory of allergic mediators, that is, effectively inhibiting the production and release of allergic mediators in all links of the allergic reaction mechanism. Compared with Western medicines such as diphenhydramine, the mechanism of action of Chinese herbal medicines is multi-level and multi-target, and can be reflected in many links of the theory of allergic mediators, such as increasing the level of intracellular AMP, stabilizing the cell membrane, inhibiting or reducing the release of allergic mediators, neutralizing antigens, inhibiting the formation of IgE, etc. The flavonoids, polysaccharides, alkaloids, α-linolenic acid, terpenes and volatile oils in plants have anti-inflammatory and anti-allergic effects. In addition, the anti-allergic active ingredients of natural plants have small toxic and side effects, generally do not cause drug dependence, and are easily accepted and recognized by patients.

[0004] At present, "pure natural, zero additives, zero stimulation" is the goal pursued by major cosmetics companies. Developing active substances with anti-allergic, anti-itching and repairing effects from natural plants, adding them to daily skin care products, and developing natural anti-allergic cosmetics has become one of the key research contents of the cosmetics industry.

[0005] After a lot of work in the field of anti-allergic repair, the applicant of the present invention developed a three-chrysanthemum anti-allergic repair agent, applied for invention patent No. CN 2019107758959, and then launched the product to the market, which was warmly welcomed. Based on this, in order to launch a product with better effect to the market, the applicant of the present invention continued to research in the above field, and due to the great improvement of the extraction process, the optimized three-chrysanthemum anti-allergic repair agent obtained has significantly improved skin care effect, thus completing the present invention. Summary of the invention

[0006] The present invention provides a three-chrysanthemum anti-allergic repair agent, and the specific technical scheme is as follows:

[0007] A three-chrysanthemum anti-allergy repair agent, comprising a feverfew (CHRYSANTHEMUM PARTHENIUM) extract, a cornflower (CENTAUREA CYANUS) extract, and a French helichrysanthemum (HELICHRYSUM STOECHAS) extract, wherein the three-chrysanthemum anti-allergy repair agent is prepared by the following process:

[0008] (1) Weigh the raw materials according to the following weight ratio: 20-50 parts of feverfew, 1-5 parts of cornflower, and 20-50 parts of French helichrysum, mix well, and crush;

[0009] (2) Extraction of the oily component: the raw material and solvent A are mixed at a material-liquid ratio of 1:5-10 m / m, stirred for extraction, and filtered to obtain a filtrate and a filter residue; then solvent B and the filtrate are added to an extractor at a weight ratio of 1:1-5, allowed to stand for stratification, the clear liquid is removed, and the residual solvent A is removed by rotary evaporation to obtain the oily component;

[0010] The solvent A of the present invention can be a non-toxic and harmless organic solvent in the art, including but not limited to alcohols, ethers, ketones, esters, aromatic hydrocarbons, halogenated hydrocarbons, for example: one or a mixture of more than one of dichloromethane, chloroform, methanol, ethanol, petroleum ether, acetone, and ethyl acetate. In some embodiments of the present invention, the solvent A is chloroform.

[0011] The temperature, time and material-liquid ratio of the extraction in step (2) have an important influence on the extraction efficiency of the active substance. In some embodiments of the present invention, the stirring extraction conditions are: 20-30°C, 50-100rpm stirring speed for 20-30 hours. The material-liquid ratio in the present invention is calculated by weight. A suitable material-liquid ratio can make the solvent and the raw material react more fully, thereby improving the extraction efficiency, avoiding waste, and reducing costs.

[0012] The solvent B of the present invention can be non-toxic and harmless oils in the art, including but not limited to hydrocarbon oils, synthetic fatty acid esters, animal and vegetable oils, silicone oils and mixtures thereof, for example: isododecane, white mineral oil, caprylic / capric triglyceride, isopropyl myristate, isononyl isononanoate, evening primrose oil, rosehip oil, avocado oil, jojoba oil, grape seed oil, white meadowfoam seed oil, squalane, phenyl trimethicone, octyl polymethicone, one or more mixtures thereof. In some embodiments of the present invention, the solvent B is squalane.

[0013] (3) Extraction of aqueous components: enzymatically hydrolyze the filter residue from step (2), then add solvent C, perform ultrasonic extraction under stirring, perform refrigerated centrifugation, and collect the centrifuge to obtain the aqueous component;

[0014] According to the present invention, the filter residue can be used directly in the subsequent steps, or it can be used in the subsequent steps after being treated by a drying method in the art. The drying method includes but is not limited to freeze drying, rotary evaporation drying, spray drying, etc. In some embodiments of the present invention, a rotary evaporation drying method is used, in a water bath at 45-55 degrees, vacuumized to -0.05 to -0.1 MPa, and rotary evaporated to dryness.

[0015] The present invention unexpectedly found that the enzymatic hydrolysis effect of the filter residue after the organic solvent extraction in step (2) is better, especially having a great influence on the yield of flavonoid active substances. The inventor speculates that it may be because the organic solvent extracts the fat-soluble components such as fatty acids, sterols, lipopolysaccharides, lipoproteins, etc. in the plant raw materials, causing the permeability of the plant cell membrane to increase, or even disintegrate, which is conducive to the full contact and action of the biological enzyme.

[0016] The biological enzyme used in the enzymolysis of the present invention is a snail mixed enzyme, which is a mixed enzyme prepared from the snail's sac and digestive tract, and contains more than 20 enzymes such as cellulase, pectinase, amylase, protease, etc. A suitable snail mixed enzyme can be snail enzyme freeze-dried powder from Ye's Biotechnology. In some embodiments of the present invention, the amount of the snail mixed enzyme is 0.1-0.5% of the weight of the filter residue.

[0017] According to the present invention, the enzymatic hydrolysis step is: adding 5-10 times the weight of water to the filter residue, then adding snail enzyme mixture, adjusting the pH to 6.0-7.0, the temperature to 35-38°C, reacting for 4-8 hours under slow stirring, heating to inactivate the enzyme, and obtaining an enzymatic hydrolysis product.

[0018] The solvent C described in the present invention can be a non-toxic and harmless polyol in the art, such as glycerol, diglycerol, 1,3-propylene glycol, 1,2-propylene glycol, butylene glycol, pentanediol, dipropylene glycol, hexylene glycol, sorbitol, polyethylene glycol-8, glycerol polyether-26, polyglycerol-8, polyglycerol-10, etc., or a mixture of more than one thereof. In some embodiments of the present invention, the solvent C is butylene glycol, and the amount of butylene glycol added is 1-3 times the weight of the filter residue.

[0019] According to the present invention, the ultrasonic extraction is as follows: turning on 50-100kHz ultrasonic waves for 30-60 minutes.

[0020] According to the present invention, the centrifugation can be carried out by a plate and frame centrifuge, a tubular centrifuge, or a desktop centrifuge, and the freezing refers to pre-freezing the feed liquid to 1-5°C, the purpose of which is to reduce the solubility of tiny particles in the feed liquid in the solution and separate them by centrifugal force, thereby avoiding slow precipitation during the later storage process.

[0021] (4) Adding polyglycerol-2 oleate and polyglycerol-6 caprylate to the oily component; adding lecithin to the aqueous component; heating and stirring to dissolve them uniformly, and mixing the two phases; ultrasonic shearing and emulsification to make colostrum, and then ultra-high pressure homogenization, filtering the material, and obtaining the three chrysanthemum anti-allergic repair agent.

[0022] In the present invention, polyglycerol-2 oleate, polyglycerol-6 caprylate and lecithin are emulsifiers. The inventors of the present invention were pleasantly surprised to find that the two originally incompatible active substances, the oily component and the aqueous component, can be added with the above-mentioned emulsifier and a high-pressure homogenization process to prepare a transparent liposome. On the one hand, the liposomes make the oil-soluble active substances and the water-soluble active substances in the extract compatible in one product, greatly improving the extraction efficiency of plant active ingredients, and the obtained extract is stable in nature and has excellent compatibility with other raw materials of cosmetics. On the other hand, the prepared liposomes have a small particle size and strong skin affinity. When used in skin care products, they can effectively pass through the skin barrier and exert a better skin care effect.

[0023] In some of the embodiments, the mass percentage of each component is: oily component 10-20%, polyglycerol-2 oleate 0.1-1%, polyglycerol-6 caprylate 1-5%, lecithin 1-5%, and aqueous component balance.

[0024] The ultra-high pressure homogenization described in the present invention refers to the use of a high pressure microfluidic device to perform cyclic homogenization twice at a pressure of 20,000 psi.

[0025] A cosmetic is prepared by adding the above-mentioned chrysanthemum anti-allergic repair agent and auxiliary materials or auxiliary ingredients in the field of cosmetics. The cosmetic is a cream, lotion, essence, facial mask, toner.

[0026] Compared with the prior art, the present invention has the following advantages: the present invention provides a three-chrysanthemum anti-allergic repair agent, which fully extracts the oil-soluble components and water-soluble components of feverfew, cornflower, and French immortelle, and adopts a liposome preparation form, which is easier to penetrate the skin barrier, and the anti-allergic repair effect is more comprehensively and deeply improved and optimized. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 Ear swelling of mice in each test group. DETAILED DESCRIPTION

[0028] The present invention discloses a three-chrysanthemum anti-allergic repair agent. Those skilled in the art can refer to the content of this article and appropriately improve the process parameters to achieve it. It is particularly important to point out that all similar substitutions and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the methods and applications described herein without departing from the content, spirit and scope of the present invention to implement and apply the technology of the present invention.

[0029] The raw materials and reagents used in the chrysanthemum anti-allergic repair agent provided by the invention can be purchased from the market.

[0030] The present invention will be further described below in conjunction with embodiments:

[0031] Example 1

[0032] A three-chrysanthemum anti-allergic repair agent is prepared by the following process:

[0033] 1. Weigh the raw materials according to the following weight ratio: 20 parts of feverfew, 5 parts of cornflower, and 20 parts of French immortelle, mix well, and crush;

[0034] 2. Extraction of oily components: Mix the raw materials and chloroform at a material-liquid ratio of 1:10 m / m, extract at 20°C and a stirring speed of 100 rpm for 20 hours, and filter to obtain a filtrate and a residue; then put squalane and the filtrate into an extractor at a weight ratio of 1:5, let stand to separate, take out the clear liquid, and remove the residual chloroform by rotary evaporation to obtain the oily component;

[0035] 3. Extraction of aqueous components: Rotate and evaporate the filter residue from step (2) to dryness in a 45°C water bath under vacuum conditions of -0.1 MPa; then add water 5 times the weight of the filter residue, add snail enzyme mix, adjust the pH to 7.0, set the temperature to 35°C, react for 8 hours under slow stirring, heat to inactivate the enzyme, and obtain an enzymatic hydrolyzate; then add butanediol 1 times the weight of the filter residue, turn on 100kHz ultrasonic treatment for 30 minutes, perform ultrasonic extraction under stirring, pre-freeze to 5°C, and centrifuge to obtain the centrifuge to obtain the aqueous component;

[0036] 4. Weigh the following components in percentage by mass: 10% of oily component, 0.1% of polyglycerol-2 oleate, 1% of polyglycerol-6 caprylate, 1% of lecithin, and the remainder of aqueous component; add polyglycerol-2 oleate and polyglycerol-6 caprylate to the oily component; add lecithin to the aqueous component; heat and stir to dissolve them uniformly and mix the two phases; emulsify them by ultrasonic shearing to make colostrum, and then homogenize them by ultrahigh pressure, filter the material, and then use a high-pressure microfluidizer to circulate and homogenize them twice at a pressure of 20,000 psi, filter the material, and then obtain the Sanju anti-allergic repair agent.

[0037] Example 2

[0038] A three-chrysanthemum anti-allergic repair agent is prepared by the following process:

[0039] 1. Weigh the following raw materials according to the following weight ratio: 50 parts of feverfew, 1 part of cornflower, and 50 parts of French immortelle, mix well, and crush;

[0040] 2. Extraction of oily components: Mix the raw materials and chloroform at a material-liquid ratio of 1:5 m / m, extract at 30°C and a stirring speed of 50 rpm for 30 hours, and filter to obtain a filtrate and a filter residue; then put squalane and the filtrate into an extractor at a weight ratio of 1:1, let stand to separate, take out the clear liquid, and remove the residual chloroform by rotary evaporation to obtain the oily component;

[0041] 3. Extraction of aqueous components: Rotate and evaporate the filter residue from step (2) to dryness in a 55°C water bath under vacuum conditions of -0.05MPa; then add water 10 times the weight of the filter residue, add snail enzyme mix, adjust the pH to 6.0, set the temperature to 38°C, react for 4 hours under slow stirring, heat to inactivate the enzyme, and obtain an enzymatic hydrolyzate; then add butanediol 3 times the weight of the filter residue, turn on 50kHz ultrasonic treatment for 60 minutes, perform ultrasonic extraction under stirring, pre-freeze to 1°C, and centrifuge to obtain the centrifuge liquid to obtain the aqueous component;

[0042] 4. Weigh the following components in percentage by mass: 20% of oily component, 1% of polyglycerol-2 oleate, 5% of polyglycerol-6 caprylate, 5% of lecithin, and the remainder of aqueous component; add polyglycerol-2 oleate and polyglycerol-6 caprylate to the oily component; add lecithin to the aqueous component; heat and stir to dissolve them uniformly and mix the two phases; emulsify them by ultrasonic shearing to make colostrum, and then homogenize them by ultrahigh pressure, filter the material, and then use a high-pressure microfluidizer to circulate and homogenize them twice at a pressure of 20,000 psi, filter the material, and then obtain the Sanju anti-allergic repair agent.

[0043] Example 3

[0044] A three-chrysanthemum anti-allergic repair agent is prepared by the following process:

[0045] 1. Weigh the raw materials according to the following weight ratio: 40 parts of feverfew, 3 parts of cornflower, and 30 parts of French immortelle, mix well, and crush;

[0046] 2. Extraction of oily components: Mix the raw materials and chloroform at a material-liquid ratio of 1:8 m / m, extract at 25°C and a stirring speed of 80 rpm for 25 hours, and filter to obtain a filtrate and a residue; then put squalane and the filtrate into an extractor at a weight ratio of 1:3, let stand to separate, take out the clear liquid, and remove the residual chloroform by rotary evaporation to obtain the oily component;

[0047] 3. Extraction of aqueous components: Rotate and evaporate the filter residue from step (2) to dryness in a 50-degree water bath under vacuum conditions of -0.08 MPa; then add water 7 times the weight of the filter residue, add snail enzyme mix, adjust the pH to 6.5, set the temperature to 36°C, react for 6 hours under slow stirring, heat to inactivate the enzyme, and obtain an enzymatic hydrolyzate; then add butanediol 2 times the weight of the filter residue, turn on 70kHz ultrasonic treatment for 50 minutes, perform ultrasonic extraction under stirring, pre-freeze to 3°C, and centrifuge to obtain the centrifuge liquid to obtain the aqueous component;

[0048] 4. Weigh the following components in percentage by mass: 15% of oily component, 0.5% of polyglycerol-2 oleate, 3% of polyglycerol-6 caprylate, 4% of lecithin, and the remainder of aqueous component; add polyglycerol-2 oleate and polyglycerol-6 caprylate to the oily component; add lecithin to the aqueous component; heat and stir to dissolve them uniformly and mix the two phases; emulsify them by ultrasonic shearing to make colostrum, and then homogenize them by ultrahigh pressure, filter the material, and then use a high-pressure microfluidizer to circulate and homogenize them twice at a pressure of 20,000 psi, filter the material, and then obtain the Sanju anti-allergic repair agent.

[0049] Comparative Example 1

[0050] A three-chrysanthemum anti-allergic repair agent is prepared by the following process:

[0051] 1. Weigh the raw materials according to the following weight ratio: 40 parts of feverfew, 3 parts of cornflower, and 30 parts of French immortelle, mix well, and crush to obtain plant powder;

[0052] 2. Add 7 times the weight of the plant powder to the plant powder. The extraction solvent is a mixture of water, butanediol and hexanediol, and the mass ratio of water, butanediol and hexanediol is 70:25:5. Heat to 85°C, extract for 2 hours under stirring, coarse filter with 100-mesh filter cloth, centrifuge to obtain the supernatant, fine filter with 1000-mesh filter cloth, collect the filtrate, and obtain the anti-allergic repair agent.

[0053] Comparative Example 2

[0054] A three-chrysanthemum anti-allergic repair agent is prepared by the following process:

[0055] 1. Weigh the raw materials according to the following weight ratio: 40 parts of feverfew, 3 parts of cornflower, and 30 parts of French immortelle, mix well, and crush;

[0056] 2. Extraction of oily components: Mix the raw materials and chloroform at a material-liquid ratio of 1:8 m / m, extract at 25°C and a stirring speed of 80 rpm for 25 hours, and filter to obtain a filtrate and a residue; then put squalane and the filtrate into an extractor at a weight ratio of 1:3, let stand to separate, take out the clear liquid, and remove the residual chloroform by rotary evaporation to obtain the oily component;

[0057] 3. Extraction of aqueous components: Rotate and evaporate the filter residue from step (2) to dryness in a 50°C water bath under vacuum -0.08MPa; then add water 7 times the weight of the filter residue, adjust the pH to 6.5, set the temperature to 36°C, and react for 6 hours under slow stirring; then add butanediol 2 times the weight of the filter residue, turn on 70kHz ultrasonic wave for 50 minutes, perform ultrasonic extraction under stirring, pre-freeze to 3°C, and centrifuge to obtain the centrifuge liquid to obtain the aqueous component;

[0058] 4. Weigh the following components in percentage by mass: 15% of oily component, 0.5% of polyglycerol-2 oleate, 3% of polyglycerol-6 caprylate, 4% of lecithin, and the remainder of aqueous component; add polyglycerol-2 oleate and polyglycerol-6 caprylate to the oily component; add lecithin to the aqueous component; heat and stir to dissolve them uniformly and mix the two phases; emulsify them by ultrasonic shearing to make colostrum, and then homogenize them by ultrahigh pressure, filter the material, and then use a high-pressure microfluidizer to circulate and homogenize them twice at a pressure of 20,000 psi, filter the material, and then obtain the Sanju anti-allergic repair agent.

[0059] Comparative Example 3

[0060] A three-chrysanthemum anti-allergic repair agent is prepared by the following process:

[0061] 1. Weigh the raw materials according to the following weight ratio: 73 parts of French immortelle, mix well, and crush;

[0062] 2. Extraction of oily components: Mix the raw materials and chloroform at a material-liquid ratio of 1:8 m / m, extract at 25°C and a stirring speed of 80 rpm for 25 hours, and filter to obtain a filtrate and a residue; then put squalane and the filtrate into an extractor at a weight ratio of 1:3, let stand to separate, take out the clear liquid, and remove the residual chloroform by rotary evaporation to obtain the oily component;

[0063] 3. Extraction of aqueous components: Rotate and evaporate the filter residue from step (2) to dryness in a 50-degree water bath under vacuum conditions of -0.08 MPa; then add water 7 times the weight of the filter residue, add snail enzyme mix, adjust the pH to 6.5, set the temperature to 36°C, react for 6 hours under slow stirring, heat to inactivate the enzyme, and obtain an enzymatic hydrolyzate; then add butanediol 2 times the weight of the filter residue, turn on 70kHz ultrasonic treatment for 50 minutes, perform ultrasonic extraction under stirring, pre-freeze to 3°C, and centrifuge to obtain the centrifuge liquid to obtain the aqueous component;

[0064] 4. Weigh the following components in percentage by mass: 15% of oily component, 0.5% of polyglycerol-2 oleate, 3% of polyglycerol-6 caprylate, 4% of lecithin, and the remainder of aqueous component; add polyglycerol-2 oleate and polyglycerol-6 caprylate to the oily component; add lecithin to the aqueous component; heat and stir to dissolve them uniformly and mix the two phases; emulsify them by ultrasonic shearing to make colostrum, and then homogenize them by ultrahigh pressure, filter the material, and then use a high-pressure microfluidizer to circulate and homogenize them twice at a pressure of 20,000 psi, filter the material, and then obtain the Sanju anti-allergic repair agent.

[0065] Comparative Example 4

[0066] A three-chrysanthemum anti-allergic repair agent is prepared by the following process:

[0067] 1. Weigh the raw materials according to the following weight ratio, including 73 parts of cornflower, mix well, and crush;

[0068] 2. Extraction of oily components: Mix the raw materials and chloroform at a material-liquid ratio of 1:8 m / m, extract at 25°C and a stirring speed of 80 rpm for 25 hours, and filter to obtain a filtrate and a residue; then put squalane and the filtrate into an extractor at a weight ratio of 1:3, let stand to separate, take out the clear liquid, and remove the residual chloroform by rotary evaporation to obtain the oily component;

[0069] 3. Extraction of aqueous components: Rotate and evaporate the filter residue from step (2) to dryness in a 50-degree water bath under vacuum conditions of -0.08 MPa; then add water 7 times the weight of the filter residue, add snail enzyme mix, adjust the pH to 6.5, set the temperature to 36°C, react for 6 hours under slow stirring, heat to inactivate the enzyme, and obtain an enzymatic hydrolyzate; then add butanediol 2 times the weight of the filter residue, turn on 70kHz ultrasonic treatment for 50 minutes, perform ultrasonic extraction under stirring, pre-freeze to 3°C, and centrifuge to obtain the centrifuge liquid to obtain the aqueous component;

[0070] 4. Weigh the following components in percentage by mass: 15% of oily component, 0.5% of polyglycerol-2 oleate, 3% of polyglycerol-6 caprylate, 4% of lecithin, and the remainder of aqueous component; add polyglycerol-2 oleate and polyglycerol-6 caprylate to the oily component; add lecithin to the aqueous component; heat and stir to dissolve them uniformly and mix the two phases; emulsify them by ultrasonic shearing to make colostrum, and then homogenize them by ultrahigh pressure, filter the material, and then use a high-pressure microfluidizer to circulate and homogenize them twice at a pressure of 20,000 psi, filter the material, and then obtain the Sanju anti-allergic repair agent.

[0071] Comparative Example 5

[0072] A three-chrysanthemum anti-allergic repair agent is prepared by the following process:

[0073] 1. Weigh the raw materials according to the following weight ratio, including 43 parts of feverfew, mix well, and crush;

[0074] 2. Extraction of oily components: Mix the raw materials and chloroform at a material-liquid ratio of 1:8 m / m, extract at 25°C and a stirring speed of 80 rpm for 25 hours, and filter to obtain a filtrate and a residue; then put squalane and the filtrate into an extractor at a weight ratio of 1:3, let stand to separate, take out the clear liquid, and remove the residual chloroform by rotary evaporation to obtain the oily component;

[0075] 3. Extraction of aqueous components: Rotate and evaporate the filter residue from step (2) to dryness in a 50-degree water bath under vacuum conditions of -0.08 MPa; then add water 7 times the weight of the filter residue, add snail enzyme mix, adjust the pH to 6.5, set the temperature to 36°C, react for 6 hours under slow stirring, heat to inactivate the enzyme, and obtain an enzymatic hydrolyzate; then add butanediol 2 times the weight of the filter residue, turn on 70kHz ultrasonic treatment for 50 minutes, perform ultrasonic extraction under stirring, pre-freeze to 3°C, and centrifuge to obtain the centrifuge liquid to obtain the aqueous component;

[0076] 4. Weigh the following components in percentage by mass: 15% of oily component, 0.5% of polyglycerol-2 oleate, 3% of polyglycerol-6 caprylate, 4% of lecithin, and the remainder of aqueous component; add polyglycerol-2 oleate and polyglycerol-6 caprylate to the oily component; add lecithin to the aqueous component; heat and stir to dissolve them uniformly and mix the two phases; emulsify them by ultrasonic shearing to make colostrum, and then homogenize them by ultrahigh pressure, filter the material, and then use a high-pressure microfluidizer to circulate and homogenize them twice at a pressure of 20,000 psi, filter the material, and then obtain the Sanju anti-allergic repair agent.

[0077] Example 4 Anti-allergic and anti-inflammatory test

[0078] The NF-κB signaling pathway plays an important role in the immunological function of keratinocytes. When the cells are not stimulated, NF-κB binds to the nuclear factor κB inhibitor protein to form an inactive complex, which is stored in the cytoplasm. When the cells are stimulated, the extracellular signal factors bind to the receptors on the cell membrane. After the receptor protein is stimulated, it first activates IκB kinase. Under the action of kinase, the complex formed by NF-κB and nuclear factor κB inhibitor protein is enzymatically hydrolyzed, releasing free NF-κB to enter the cell nucleus, initiating the transcription process and activating the expression of immune-related cytokines and adhesion factors.

[0079] As a protein kinase activator and known skin tumor promoter, phorbol ester can activate IκB kinase and cause skin irritation. After the skin comes into contact with phorbol ester, an inflammatory reaction may occur, causing the skin to produce a large amount of inflammatory mediators, leading to erythema and edema of the skin. Therefore, phorbol ester is a commonly used anti-inflammatory substance for constructing a mouse skin inflammation model. This model is widely used in the screening of anti-inflammatory effects of compounds and plant extracts. Compared with conventional in vitro methods, such as hyaluronidase inhibition tests, the phorbol ester mouse skin inflammation model has a clearer mechanism of action, involves more types of inflammatory factors, simulates the physiological environment of the human body and has strong data extrapolation, and is a better anti-allergic and anti-inflammatory model.

[0080] The three ju anti-allergic repair agents prepared in Examples 1-3 and Comparative Examples 1-5 were tested on the phorbol ester mouse skin inflammation model. The specific test method was referred to the literature "Zhou Qinchun, Xu Deping. Anti-inflammatory Activity and Components of Papaya [J / OL]. Food and Fermentation Industry, 1-10 [2024-11-09]. ". The ear swelling degree of mice in each test group was calculated, as shown in detail. Figure 1 .

[0081] Depend on Figure 1 It can be seen that the Sanju anti-allergic repair agent prepared in the embodiment of the present invention shows a good anti-inflammatory effect.

[0082] Example 5

[0083] An anti-allergic repairing facial mask comprises the following components in percentage by weight: 5% glycerin, 2% squalane, 1% isononyl isononanoate, 0.5% panthenol, 0.5% polyglyceryl-10 myristate, 0.5% 1,2-hexanediol, 0.5% p-hydroxyacetophenone, 5% of the chrysanthemum antiallergic agent prepared in Example 1, and the balance water.

[0084] Example 6

[0085] An anti-allergic repair cream comprises the following components in percentage by weight: 3% of cetearyl alcohol, 1% of beeswax, 5% of butyrospermum parkii fruit oil, 3% of polydimethylsiloxane, 1% of hydrogenated palm kernel glycerides, 1% of hydrogenated palm glycerides, 1% of cetearyl glucoside, 1% of coconut glucoside, 3% of diglycerol, 5% of glycerol, 0.5% of acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, 0.3% of aminomethyl propanediol, 10% of the chrysanthemum antiallergic agent prepared in Example 3, and the balance of water.

[0086] Example 7

[0087] 30 volunteers who self-evaluated their skin as sensitive were recruited to use the anti-allergic repair cream prepared in Example 6. The test sample was applied to the face once in the morning and evening, with an application amount of 0.1 g / 10 cm each time.2 . The results were statistically analyzed after 1 month of continuous use.

[0088] The evaluation criteria for the volunteer test were: markedly effective means that symptoms such as tightness, erythema, flushing, papules, stinging, itching, and desquamation were significantly improved; effectively means that symptoms such as tightness, erythema, flushing, papules, stinging, itching, and desquamation were alleviated to a certain extent; the rest were ineffective.

[0089] According to statistics, 15 volunteers were markedly effective, 10 volunteers were effective, and the remaining 5 were ineffective. The total effective rate reached more than 80%. It can be seen that the anti-allergic repair cream prepared in the embodiment of the present invention achieves a good anti-allergic repair effect.

[0090] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A three-chrysanthemum anti-allergy repair agent, comprising a feverfew (CHRYSANTHEMUM PARTHENIUM) extract, a cornflower (CENTAUREA CYANUS) extract, and a French helichrysanthemum (HELICHRYSUM STOECHAS) extract, wherein the three-chrysanthemum anti-allergy repair agent is prepared by the following process: (1) Weigh the raw materials according to the following weight ratio: 20-50 parts of feverfew, 1-5 parts of cornflower, and 20-50 parts of French helichrysum, mix well, and crush; (2) Extraction of the oily component: the raw material and solvent A are mixed at a material-liquid ratio of 1:5-10 m / m, stirred for extraction, and filtered to obtain a filtrate and a filter residue; then solvent B and the filtrate are added to an extractor at a weight ratio of 1:1-5, allowed to stand for stratification, the clear liquid is removed, and the residual solvent A is removed by rotary evaporation to obtain the oily component; (3) Extraction of aqueous components: enzymatically hydrolyze the filter residue from step (2), then add solvent C, perform ultrasonic extraction under stirring, perform refrigerated centrifugation, and collect the centrifuge to obtain the aqueous component; (4) Adding polyglycerol-2 oleate and polyglycerol-6 caprylate to the oily component; adding lecithin to the aqueous component; heating and stirring to dissolve them uniformly, and mixing the two phases; ultrasonic shearing and emulsification to make colostrum, and then ultra-high pressure homogenization, filtering the material, and obtaining the three chrysanthemum anti-allergic repair agent.

2. The chrysanthemum anti-allergic repair agent according to claim 1, characterized in that: The solvent A in step (2) is chloroform, and the solvent B is squalane.

3. The chrysanthemum anti-allergic repair agent according to claim 1, characterized in that: The stirring extraction conditions described in step (2) are: extraction at 20-30° C. and a stirring speed of 50-100 rpm for 20-30 hours.

4. The chrysanthemum anti-allergic repair agent according to claim 1, characterized in that: The enzymatic hydrolysis step described in step (3) is: adding 5-10 times the weight of water to the filter residue, then adding snail enzyme mixture, adjusting the pH to 6.0-7.0, the temperature to 35-38°C, reacting for 4-8 hours under slow stirring, heating to inactivate the enzyme, and obtaining an enzymatic hydrolysis product.

5. The chrysanthemum anti-allergic repair agent according to claim 1, characterized in that: The solvent C described in step (3) is butanediol, and the amount of butanediol added is 1-3 times the weight of the filter residue.

6. The chrysanthemum anti-allergic repair agent according to claim 1, characterized in that: The ultrasonic extraction described in step (3) is as follows: turning on 50-100kHz ultrasonic waves for 30-60 minutes.

7. The chrysanthemum anti-allergic repair agent according to claim 1, characterized in that: The freezing described in step (3) refers to pre-freezing the liquid to 1-5°C.

8. The chrysanthemum anti-allergic repair agent according to claim 1, characterized in that: The mass percentages of the components in step (4) are: 10-20% of oily component, 0.1-1% of polyglycerol-2 oleate, 1-5% of polyglycerol-6 caprylate, 1-5% of lecithin, and the balance of aqueous component.

9. The chrysanthemum anti-allergic repair agent according to claim 1, characterized in that: The ultra-high pressure homogenization described in step (4) refers to cyclic homogenization twice at a pressure of 20,000 psi using a high pressure microfluidizer.

10. Use of the Sanju anti-allergic repair agent according to any one of claims 1 to 9 in the preparation of cosmetics.

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