Hydrogen-rich water mask, preparation method thereof and application of hydrogen-rich water mask to chloasma

Abstract

The invention belongs to the technical field of masks, and provides a hydrogen-rich water mask, a preparation method thereof and application of the hydrogen-rich water mask to chloasma. The hydrogen-rich water mask is prepared from the following raw materials: hydrogen generating powder and essence, the essence is prepared from a periplaneta americana extract, a pomegranate peel extract and collagen. The preparation method comprises the following steps: (1) adding periplaneta americana extract, pomegranate peel extract, collagen, deionized water, glycerol, xanthan gum, polysorbate-80, hydroxyethyl cellulose and methylparaben in parts by weight, slowly dropwise adding glyceryl alcohol while mixing, and adjusting the pH value to obtain essence; and (2) sub-packaging the hydrogen generating powder, the essence and the mask paper into mask bags. The hydrogen-rich water mask is safe and free of use dependence, has strong anti-aging, whitening, repairing and freckle-removing effects, and can be used for preparing products for fading or removing chloasma. The preparation method is simple and easy to operate and can be suitable for industrial production.

Description

Technical Field

[0001] The invention belongs to the technical field of facial masks, and in particular relates to a hydrogen-rich water facial mask, a preparation method thereof, and application thereof on chloasma. Background Art

[0002] Hydrogen is a natural antioxidant, the smallest and lightest element in nature. Its following characteristics determine that it has a powerful whitening and freckle removal effect that other antioxidants cannot match, and its impact on the human body is also very small: First, as the smallest substance in the universe, hydrogen has strong penetrability. It can quickly penetrate hydrogen into all layers of skin cells and mitochondria, activating the body's antioxidant mechanism; secondly, hydrogen can quickly remove harmful reactive oxygen free radicals, reduce the production of reactive oxygen and oxidative stress damage, and accelerate the repair of damaged skin tissue; thirdly, hydrogen enhances the metabolic function of cells, activates skin cells, and reduces the stimulation of reactive oxygen to melanocytes, achieving highly effective anti-aging, freckle removal and whitening effects.

[0003] American cockroach is a traditional Chinese medicine, which was first recorded in Shennong's Herbal Classic. It has the effects of promoting blood circulation, removing blood stasis, detoxifying and eliminating malnutrition. Modern research has shown that American cockroach is rich in protein and amino acids, with a protein content of up to 57%, containing more than 17 kinds of amino acids, of which 7 are essential amino acids for the human body and 2 are semi-essential amino acids; peptides, more than 50 kinds of neuropeptides have been isolated and identified, of which the content of unsaturated fatty acids is as high as 71.9%; carbohydrates; enzymes; pheromones; vitamins (B1, B2, C), cytochromes (a, b, c), coenzyme A, chitin, sclerenchyma, chitin and calcium, magnesium, iron, zinc and other essential substances for the human body.

[0004] The most familiar American cockroach extract product is Kangfuxin Liquid, which is a biological preparation separated and refined from the American cockroach extract. Kangfuxin Liquid is rich in polyols and peptides, which can promote the growth of epidermal cells and the proliferation of granulation tissue, improve local blood circulation, promote the shedding of necrotic tissue on the wound surface, and accelerate wound repair. Kangfuxin Liquid also contains mucosine, which can enhance the phagocytic ability of macrophages and NK cells (natural killer cells), secrete interleukins, interferons, leukotrienes and other substances, and eliminate inflammatory edema. In addition to Kangfuxin Liquid, according to the currently available literature, there are also many applications of American cockroach extracts for repair and anti-inflammatory aspects.

[0005] Pomegranate is rich in polyphenol compounds, which have strong antioxidant effects and can prevent free radicals from damaging body cells; the various compounds contained in pomegranate have anti-inflammatory effects; pomegranate filtrate is rich in cellulose and tannic acid, which helps promote digestion and prevent indigestion problems such as constipation and excessive stomach acid; in addition, pomegranate also contains a variety of vitamins and minerals.

[0006] Pomegranate peel extract contains antioxidant ingredients such as vitamin C, vitamin E, pomegranate polyphenols, etc., which can prevent free radicals from damaging the skin, promote skin cell regeneration, and prevent aging; it contains anthocyanins, vitamins and other ingredients, which can remove free radicals from the skin, inhibit the production of melanocytes, and help dull skin and other skin conditions; it can enhance the skin's epidermal cell regeneration ability, prevent skin damage, and establish a protection system for the fragile skin barrier; it can improve poor blood circulation in the skin, promote skin metabolism, and thereby improve problems such as dull skin tone and spots; it has antibacterial properties, which can help the skin fight against fungi, bacteria and other substances, and thereby prevent skin diseases such as acne and seborrheic dermatitis.

[0007] Melasma, also known as liver spots or pregnancy spots, is a common pigmentation skin disease. Melasma is mainly manifested as yellow-brown to dark-brown patches with clear boundaries on the facial skin. It is more common in women, especially pregnant women and women of childbearing age. The formation of melasma is related to many factors, including genetic tendency, ultraviolet radiation, endocrine changes, and side effects of certain drugs. Although melasma itself is not contagious, its occurrence may be closely related to individual living habits and environmental factors. Although melasma itself is not life-threatening, it may have a negative impact on the patient's quality of life, mental health, and social activities.

[0008] At present, there are various treatments for melasma, including topical medications, oral medications, laser treatment, etc. Most oral medications have side effects such as nausea, vomiting, and abnormal liver function. The drugs irritate the gastrointestinal mucosa or affect the central nervous system, which can lead to symptoms such as loss of appetite and indigestion. In severe cases, it can cause dehydration and malnutrition. In order to achieve significant whitening and freckle removal effects in a short period of time, topical medications add excessive amounts of mercury, lead, and hormones to the products. Once the medication is stopped, it will rebound immediately and cause skin inflammation. Laser treatment can easily cause skin damage, pigmentation, local scars, etc. Summary of the invention

[0009] In view of the above-mentioned deficiencies in the prior art, the first purpose of the present invention is to provide a safe and non-addictive hydrogen-rich water mask, which adopts the technology of wrapping solid hydrogen generating powder with essence to form hydrogen-rich essence. After the mask paper comes into contact with the hydrogen-rich essence for the first time, a hydrogen-rich mask is generated and applied to the face immediately. The hydrogen generating powder releases hydrogen immediately after coming into contact with water. The essence wrapping can make the hydrogen be released continuously and slowly, thereby better playing the role of anti-aging, anti-inflammatory, freckle removal and skin beautification; at the same time, the American cockroach extract, pomegranate peel extract and collagen and other ingredients in the essence can complement each other and promote each other to play a synergistic role after forming the hydrogen-rich essence. The effective ingredients can further improve the anti-aging, whitening, anti-inflammatory, repair and freckle removal effects of the hydrogen-rich water mask.

[0010] In view of the above-mentioned deficiencies in the prior art, the second object of the present invention is to provide a method for preparing a hydrogen-rich water mask, which is simple and easy to operate and suitable for industrial production.

[0011] In view of the above-mentioned deficiencies in the prior art, the third object of the present invention is to provide an application of a hydrogen-rich water mask in the preparation of a product for lightening or removing chloasma.

[0012] In order to achieve the above object, the solution adopted by the present invention is: A hydrogen-rich water facial mask, the raw materials of which include hydrogen generating powder and essence; the essence includes American cockroach extract, pomegranate peel extract and collagen.

[0013] A method for preparing the hydrogen-rich water mask as described above comprises: (1) adding American cockroach extract, pomegranate peel extract, collagen, deionized water, glycerin, xanthocollagen, polysorbate 80, hydroxyethyl cellulose and methylparaben by weight, slowly adding glycerol dropwise while mixing, and adjusting the pH value to between 6.2 and 6.4 to obtain an essence; (2) packing the hydrogen generating powder, the essence and the mask paper into a mask bag, and evacuating the part containing the hydrogen generating powder to obtain the mask.

[0014] The use of the hydrogen-rich water mask in preparing products for lightening or removing chloasma.

[0015] The hydrogen-rich water mask provided by the present invention and its preparation method and its beneficial effects in treating chloasma are: (1) The hydrogen-rich water mask provided by the present invention is to mix the essence and the hydrogen generating powder and then immediately place them on the mask paper for application. The technology of essence wrapping the solid hydrogen generating powder is adopted to form a hydrogen-rich essence. After the mask paper contacts the hydrogen-rich essence for the first time, a hydrogen-rich mask is generated and immediately applied to the face. The hydrogen generating powder releases hydrogen immediately after contacting water, but because it is wrapped in the essence, it can slowly and continuously release hydrogen, thereby better playing the role of anti-aging, anti-inflammatory, freckle removal and skin beautification.

[0016] (2) The hydrogen-rich water mask provided by the present invention, wherein the hydrogen generating powder can release hydrogen after contacting with water, which has significant antioxidant effects, quickly removes harmful active oxygen free radicals and has whitening effects, the American cockroach extract has powerful repair and anti-inflammatory effects, the pomegranate peel extract can improve skin metabolism and at the same time can be used as waste to reduce production costs, and the collagen has the effects of firming the skin, smoothing wrinkles, whitening freckles, etc.; after forming the hydrogen-rich essence, the effective ingredients of each raw material can complement each other, promote each other to exert their effectiveness, and play a synergistic role, thereby further improving the anti-aging, whitening, anti-inflammatory, repair and freckle removal effects of the hydrogen-rich water mask. DETAILED DESCRIPTION

[0017] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be described clearly and completely below. If the specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0018] The following is a detailed description of a hydrogen-rich water facial mask provided by an embodiment of the present invention, a preparation method thereof, and its application in treating chloasma.

[0019] A hydrogen-rich water facial mask, the raw materials of which include hydrogen generating powder and essence; the essence includes American cockroach extract, pomegranate peel extract and collagen.

[0020] The hydrogen generating powder comprises magnesium powder and citric acid powder in a mass ratio of 2:9-11.

[0021] The essence comprises, by weight: 4-6 parts of American cockroach extract, 3-5 parts of pomegranate peel extract, 2-3 parts of collagen, 78-80 parts of deionized water, 10-13 parts of glycerin, 0.4-0.6 parts of xanthocollagen, 1-2 parts of polysorbate-80, 0.1-0.3 parts of hydroxyethylcellulose, 1-2 parts of methylparaben, and 4-6 parts of glycerol.

[0022] The essence within the above ratio range can have excellent ability to help skin metabolism, quickly repair damaged skin, tighten skin, etc. Glycerol can help increase the surface tension of the essence, lock and evenly disperse the active ingredients, facilitate the continuous release of the active ingredients in the mask, and also facilitate the skin's absorption of the active ingredients in the mask.

[0023] The preparation method of the American cockroach extract comprises: crushing the inactivated American cockroach into coarse powder, extracting in a supercritical carbon dioxide extraction device, controlling the pressure to 25-28MPa, the temperature to 42-45°C, the carbon dioxide flow rate to 15-18L / h, extracting for 1-1.5h, obtaining and collecting the American cockroach oil to obtain the American cockroach extract.

[0024] The American cockroach extract prepared by the preparation method of the American cockroach extract has excellent repairing, antibacterial and anti-inflammatory effects, and is beneficial to further help skin repair and improve skin metabolism.

[0025] The preparation method of pomegranate peel extract comprises: collecting pomegranate peel, cleaning it, adding 2 times water to crush it, filtering to obtain a filtrate, adding yeast to ferment the filtrate at 20-22°C for 6-8 days, collecting the fermented liquid, adding lactic acid bacteria and fermenting it again at a temperature of 15-18°C for 2-3 days, then ultrasonically extracting at a water temperature of 40-50°C for 30 minutes under normal pressure to obtain an extract; filtering the extract and concentrating it under reduced pressure to obtain the pomegranate peel extract.

[0026] In this embodiment, the usage ratio of hydrogen generating powder to essence is 1 mg:8-10 ml.

[0027] Using the hydrogen generating powder and essence within the above-mentioned ratio range will help the essence to fully wrap the hydrogen generating powder and slowly release hydrogen, thereby achieving better antioxidant, whitening and freckle removal effects.

[0028] The preparation method of the hydrogen-rich water facial mask comprises: (1) adding American cockroach extract, pomegranate peel extract, collagen, deionized water, glycerin, xanthocollagen, polysorbate-80, hydroxyethyl cellulose and methylparaben according to weight proportions, slowly adding glycerol dropwise while mixing, and adjusting the pH value to between 6.2 and 6.4 to obtain an essence; (2) packing hydrogen generating powder, essence and facial mask paper into facial mask bags, and evacuating the part containing hydrogen generating powder to obtain the facial mask.

[0029] The features and performance of the present invention are further described in detail below in conjunction with the embodiments.

[0030] Example 1 This embodiment provides a method for preparing a hydrogen-rich water mask, comprising: (1) Add 5 parts of American cockroach extract, 4 parts of pomegranate peel extract, 2.5 parts of collagen, 79 parts of deionized water, 12 parts of glycerin, 0.5 parts of xanthocollagen, 1.5 parts of polysorbate-80, 0.2 parts of hydroxyethylcellulose, and 1.5 parts of methylparaben by weight, and slowly add 5 parts of glycerol dropwise while mixing, and adjust the pH value to 6.3 to obtain an essence; The preparation method of the American cockroach extract comprises: crushing the inactivated American cockroach into coarse powder, extracting in a supercritical carbon dioxide extraction device, controlling the pressure to 26MPa, the temperature to 43°C, the carbon dioxide flow rate to 16L / h, extracting for 1.2h, obtaining and collecting the American cockroach oil to obtain the American cockroach extract.

[0031] The preparation method of pomegranate peel extract comprises: collecting pomegranate peel, cleaning it, adding 2 times water to crush it, filtering to obtain a filtrate, adding yeast to ferment the filtrate at 21°C for 7 days, collecting the fermented liquid, adding lactic acid bacteria and fermenting it again at 16°C for 2 days, then ultrasonically extracting at normal pressure at 45°C for 30 minutes to obtain an extract; filtering the extract and concentrating it under reduced pressure to obtain the pomegranate peel extract.

[0032] (2) Pack the hydrogen generating powder (including magnesium powder and citric acid powder in a mass ratio of 1:5), essence and mask paper into mask bags. The usage ratio of hydrogen generating powder to essence is 1mg:9ml, and the part containing hydrogen generating powder is evacuated.

[0033] Example 2 This embodiment provides a method for preparing a hydrogen-rich water mask, comprising: the difference from Embodiment 1 is that the hydrogen generating powder comprises: magnesium powder and citric acid powder in a mass ratio of 2:9.

[0034] Example 3 This embodiment provides a method for preparing a hydrogen-rich water mask, comprising: the difference from Embodiment 1 is that the hydrogen generating powder comprises: magnesium powder and citric acid powder in a mass ratio of 2:11.

[0035] Example 4 This embodiment provides a method for preparing a hydrogen-rich water mask, comprising: the difference from Example 1 is that the usage ratio of hydrogen generating powder and essence is 1 mg:8 ml.

[0036] Example 5 This embodiment provides a method for preparing a hydrogen-rich water mask, comprising: the difference from Example 1 is that the usage ratio of hydrogen generating powder and essence is 1 mg:10 ml.

[0037] Example 6 The present embodiment provides a method for preparing a hydrogen-rich water mask, comprising: the difference from the first embodiment is that: (1) by weight, 3 parts of American cockroach extract, 5 parts of pomegranate peel extract, 2 parts of collagen, 80 parts of deionized water, 10 parts of glycerin, 0.6 parts of xanthocollagen, 1 part of polysorbate-80, 0.3 parts of hydroxyethyl cellulose, and 1 part of methylparaben are added, and 6 parts of glycerol are slowly added dropwise while mixing, and the pH value is adjusted to between 6.3 to obtain an essence.

[0038] Example 7 The present embodiment provides a method for preparing a hydrogen-rich water mask, comprising: the difference from the first embodiment is that: (1) by weight, 6 parts of American cockroach extract, 3 parts of pomegranate peel extract, 3 parts of collagen, 78 parts of deionized water, 13 parts of glycerin, 0.4 parts of xanthocollagen, 2 parts of polysorbate-80, 0.1 parts of hydroxyethyl cellulose, and 2 parts of methylparaben are added, and 4 parts of glycerol are slowly added dropwise while mixing, and the pH value is adjusted to between 6.3 to obtain an essence.

[0039] Example 8 The present embodiment provides a method for preparing a hydrogen-rich water mask, comprising: the difference from Example 1 is that the method for preparing an American cockroach extract comprises: crushing the inactivated American cockroach into coarse powder, extracting in a supercritical carbon dioxide extraction device, controlling the pressure to 25 MPa, the temperature to 45°C, the carbon dioxide flow rate to 15 L / h, extracting for 1.5 hours, obtaining and collecting American cockroach oil to obtain an American cockroach extract.

[0040] Example 9 The present embodiment provides a method for preparing a hydrogen-rich water mask, comprising: the difference from Example 1 is that the method for preparing an American cockroach extract comprises: crushing the inactivated American cockroach into coarse powder, extracting in a supercritical carbon dioxide extraction device, controlling the pressure to 28 MPa, the temperature to 42°C, the carbon dioxide flow rate to 18 L / h, extracting for 1 hour, obtaining and collecting American cockroach oil to obtain an American cockroach extract.

[0041] Example 10 The present embodiment provides a method for preparing a hydrogen-rich water mask, comprising: the difference from Example 1 is that the method for preparing a pomegranate peel extract comprises: collecting pomegranate peels, cleaning them, adding 2 times water to crush them, filtering to obtain a filtrate, adding yeast to ferment the filtrate at 20°C for 8 days, collecting the fermented liquid, adding lactic acid bacteria and fermenting it again at a temperature of 15°C for 3 days, then ultrasonically extracting at a water temperature of 40°C for 30 minutes to obtain an extract; filtering the extract and concentrating it under reduced pressure to obtain a pomegranate peel extract.

[0042] Embodiment 11 The present embodiment provides a method for preparing a hydrogen-rich water mask, comprising: the difference from Example 1 is that the method for preparing a pomegranate peel extract comprises: collecting pomegranate peels, cleaning them, adding 2 times water to crush them, filtering to obtain a filtrate, adding yeast to ferment the filtrate at 22°C for 6 days, collecting the fermented liquid, adding lactic acid bacteria and fermenting it again at 18°C ​​for 2 days, then ultrasonically extracting at normal pressure at 50°C for 30 minutes to obtain an extract; filtering the extract and concentrating it under reduced pressure to obtain a pomegranate peel extract.

[0043] Comparative Example 1 This comparative example provides a method for preparing a hydrogen-rich water mask, comprising: the difference from Example 1 is that no hydrogen generating powder is used.

[0044] Comparative Example 2 This comparative example provides a method for preparing a hydrogen-rich water mask, comprising: the difference from Example 1 is that: (1) by weight, 78-80 parts of deionized water, 10-13 parts of glycerin, 0.4-0.6 parts of xanthan gum, 1-2 parts of polysorbate-80, 0.1-0.3 parts of hydroxyethyl cellulose, and 1-2 parts of methylparaben are added, and 4-6 parts of glycerol are slowly added dropwise while mixing, and the pH value is adjusted to between 6.2 and 6.4 to obtain an essence.

[0045] Comparative Example 3 This comparative example provides a method for preparing a hydrogen-rich water mask, comprising: the difference from Example 1 is that: (1) by weight, 3-5 parts of pomegranate peel extract, 2-3 parts of collagen, 78-80 parts of deionized water, 10-13 parts of glycerin, 0.4-0.6 parts of xanthocollagen, 1-2 parts of polysorbate-80, 0.1-0.3 parts of hydroxyethyl cellulose, and 1-2 parts of methylparaben are added, and 4-6 parts of glycerol are slowly added dropwise while mixing, and the pH value is adjusted to between 6.2 and 6.4 to obtain an essence.

[0046] Comparative Example 4 This comparative example provides a method for preparing a hydrogen-rich water mask, comprising: the difference from Example 1 is that: (1) by weight, 4-6 parts of American cockroach extract, 2-3 parts of collagen, 78-80 parts of deionized water, 10-13 parts of glycerin, 0.4-0.6 parts of xanthocollagen, 1-2 parts of polysorbate-80, 0.1-0.3 parts of hydroxyethyl cellulose, and 1-2 parts of methylparaben are added; while mixing, 4-6 parts of glycerol are slowly added dropwise; and the pH value is adjusted to between 6.2 and 6.4 to obtain an essence.

[0047] Comparative Example 5 This comparative example provides a method for preparing a hydrogen-rich water mask, comprising: the difference from Example 1 is that the hydrogen generating powder comprises: magnesium powder and citric acid powder in a mass ratio of 1:6.

[0048] Comparative Example 6 This comparative example provides a method for preparing a hydrogen-rich water mask, comprising: the difference from Example 1 is that the usage ratio of hydrogen generating powder to essence is 1 mg:11 ml.

[0049] Comparative Example 7 This comparative example provides a method for preparing a hydrogen-rich water mask, comprising: the difference from Example 1 is that: (1) by weight, 2 parts of American cockroach extract, 6 parts of pomegranate peel extract, 1 part of collagen, 81 parts of deionized water, 9 parts of glycerin, 0.7 parts of xanthocollagen, 0.8 parts of polysorbate-80, 0.4 parts of hydroxyethyl cellulose, and 0.8 parts of methylparaben are added, and 7 parts of glycerol is slowly added dropwise while mixing, and the pH value is adjusted to 6.0 to obtain an essence.

[0050] Comparative Example 8 This comparative example provides a method for preparing a hydrogen-rich water mask, comprising: the difference from Example 1 is that the method for preparing the American cockroach extract comprises: crushing the inactivated American cockroach into coarse powder, extracting in a supercritical carbon dioxide extraction device, controlling the pressure to 20 MPa, the temperature to 50°C, the carbon dioxide flow rate to 12 L / h, extracting for 2 hours, obtaining and collecting the American cockroach oil to obtain the American cockroach extract.

[0051] Comparative Example 9 The present comparative example provides a method for preparing a hydrogen-rich water mask, comprising: the method for preparing an American cockroach extract comprises: (1) mixing and soaking American cockroach dry material with water in a mass ratio of 1:10, refluxing and extracting the mixture obtained after soaking at 85°C for 3 times, each time for 2 hours, collecting the extract, filtering and concentrating the extract to a relative density of 1.10-1.20 at 65°C, and obtaining an American cockroach water extract; (2) mixing the American cockroach water extract with ethanol, adjusting the alcohol content to 70%, stirring and standing, filtering, and concentrating the obtained filtrate to a relative density of 1.05-1.10 at 65°C; discarding the upper layer of fat, filtering the lower layer of solution, and concentrating the obtained filtrate to dryness under reduced pressure to obtain an American cockroach extract.

[0052] Comparative Example 10 The present embodiment provides a method for preparing a hydrogen-rich water mask, comprising: the difference from Example 1 is that the method for preparing a pomegranate peel extract comprises: collecting pomegranate peels, cleaning them, adding 2 times water to crush them, filtering to obtain a filtrate, adding yeast to ferment the filtrate at 18°C ​​for 9 days, collecting the fermented liquid, adding lactic acid bacteria and fermenting it again at 20°C for 1 day, then ultrasonically extracting at 55°C under normal pressure for 30 minutes to obtain an extract; filtering the extract and concentrating it under reduced pressure to obtain a pomegranate peel extract.

[0053] Experimental Example 1 Tyrosinase Activity Inhibition Experiment Experimental methods: To verify the tyrosinase inhibitory efficacy of the composition, the experiment was divided into 23 groups.

[0054] Experimental group 1-11: The hydrogen generating powder and the essence in the hydrogen-rich water mask provided in Example 1-11 were mixed evenly; Control group 1-10: The hydrogen generating powder and the essence in the hydrogen-rich water mask provided in comparative examples 1-10 were mixed evenly; Positive control group: 100% arbutin; Negative control group: normal cultured B16 cells; Experimental methods: 1. Determination of cell proliferation rate (CCK8 method) Mouse melanoma B16 cells were cultured and when the cells reached the logarithmic phase, they were digested with 0.25% trypsin and the cell concentration was adjusted to 5×10 5 / mL, inoculated in a 96-well plate, 100uL per well. After culturing in an incubator for 24h, discard the supernatant, add drug-containing culture medium, and the final concentration of the sample is set to 150ul / ml. The control group does not add drugs, but instead uses the corresponding solvent. After incubation for 72h, discard the supernatant, wash twice with PBS, add 100ul / well fresh culture medium and 10ul / well CCK-8 solution, put back into the cell culture incubator, and measure A450 after 1 hour. There are 3 replicates in each group, and the experiment is repeated 3 times.

[0055] Cell proliferation rate % = (mean absorbance value of drug group / mean absorbance value of control group) × 100%.

[0056] 2. Determination of intracellular tyrosinase activity Mouse melanoma B16 cells were cultured and when the cells reached the logarithmic phase, they were digested with 0.25% trypsin and the cell concentration was adjusted to 5×10 5 / mL, inoculated in a 24-well plate, 1ml per well. After culturing in an incubator for 24h, discard the supernatant, add drug-containing culture medium, and the final concentration of the sample is set at 150ul / ml; after 3 days of drug action, discard the supernatant, rinse twice with pH7.0 PBS, and add 180uL of PBS containing 1% Triton×-100 to each well. Ultrasonic disruption and vibration in an ice bath for 5min to dissolve the cells, add 2mg / ml L-DOPA20uL to each well after preheating at 37℃, incubate at 37℃ for 60min, aspirate the reaction solution into a 96-well plate, 100ul / well, duplicate each specimen, and measure the absorbance value of each well at 450nm.

[0057] Tyrosinase synthesis inhibition rate % = (1-average absorbance value of drug group / average absorbance value of control group) × 100.

[0058] The experimental results are shown in Table 1: Table 1 Cell proliferation rate (%) and tyrosinase synthesis inhibition rate (%) Cell proliferation rate (%) Tyrosinase synthesis inhibition rate (%) Cell proliferation rate (%) Tyrosinase synthesis inhibition rate (%) Example 1 90.12 15.56 Control group 2 80.12 11.88 Example 2 89.24 15.23 Control group 3 81.34 12.02 Example 3 89.33 15.12 Control group 4 82.22 12.68 Example 4 88.14 14.92 Control group 5 83.14 13.11 Example 5 87.86 15.08 Control group 6 84.11 12.24 Example 6 88.25 15.06 Control group 7 84.08 12.02 Example 7 88.56 14.98 Control group 8 83.24 12.46 Example 8 89.01 14.68 Control group 9 83.12 12.36 Example 9 87.24 15.01 Control group 10 84.04 12.95 Example 10 88.66 14.95 Positive control group 70.12 11.25 Embodiment 11 89.22 15.12 Negative control group 100 100 Control group 1 79.28 9.24 The data in Table 1 show that the cytotoxicity of the experimental groups 1-11 is significantly better than that of arbutin widely used in the prior art, and its effect of inhibiting tyrosinase activity is also significantly better than that of arbutin widely used in the prior art; although the cytotoxicity of the comparative examples 1-10 is better than that of arbutin widely used in the prior art, and its effect of inhibiting tyrosinase activity is also better than that of arbutin widely used in the prior art, its cytotoxicity and tyrosinase inhibition activity are significantly worse than those of the experimental groups 1-11. The hydrogen-rich water mask provided in Examples 1-11 has good safety and efficacy.

[0059] Experimental Example 2 Effect of Hydrogen-Rich Water Mask on Total Color Difference of Guinea Pig UV Irradiation Model (1) Modeling: 110 4-week-old male brown guinea pigs were randomly divided into 22 groups: a blank group, Example 1-11 groups, and Comparative Example 1-10 groups. The same part of the back of each guinea pig was depilated to form two 2*2 cm areas. The total color difference (ΔE) of the two depilated areas of each guinea pig was measured using a Lab colorimeter as the initial ΔE. One of the depilated areas was irradiated with UV light once a day for 7 consecutive days at an intensity of 2 mW / cm 2 , irradiation time 10 minutes, and other areas were protected. After 7 days of irradiation, the total color difference (ΔE) of the two hair removal areas of each guinea pig was measured using a Lab colorimeter; (2) Coating: After 7 days of irradiation, the corresponding hydrogen-rich water mask of Examples 1-11 of the present invention and Comparative Examples 1-10 was applied to the UV irradiated area of ​​the guinea pig once a day for 20 minutes, and then washed with warm water. The blank group was washed with clean water for a total of 14 days; (3) Color difference measurement: 14 days after coating, the ΔE of two areas on the back of the same guinea pig was measured using a Lab colorimeter, and the ΔE reduction rate after 14 days of coating was calculated. The specific results are shown in Table 2.

[0060] ΔE reduction rate after 14 days of coating = (ΔE after 7 days of irradiation - ΔE after 14 days of coating) / ΔE after 7 days of irradiation Table 2 Effect of hydrogen-rich water mask on the total color difference of guinea pig UV irradiation model ΔE reduction rate after 14 days of coating (%) ΔE reduction rate after 14 days of coating (%) Example 1 68.28 Control group 1 18.26 Example 2 66.96 Control group 2 22.12 Example 3 67.85 Control group 3 25.46 Example 4 68.04 Control group 4 25.06 Example 5 67.94 Control group 5 30.03 Example 6 67.65 Control group 6 28.32 Example 7 66.88 Control group 7 29.42 Example 8 67.24 Control group 8 28.12 Example 9 67.46 Control group 9 29.42 Example 10 67.24 Control group 10 31.12 Embodiment 11 66.96 Blank Group 6.54 The data in Table 2 show that the skin color difference of the ultraviolet irradiation area relative to the non-ultraviolet irradiation area in Examples 1-11 is significantly reduced, which is significantly higher than the blank group. For Comparative Examples 1-10, although the skin color difference of the ultraviolet irradiation area relative to the non-ultraviolet irradiation area is reduced, and the reduction rate is higher than the blank group, it is still significantly lower than that of Examples 1-11. The results in Table 2 show that the hydrogen-rich water mask provided by the present invention has a significant freckle removal and whitening effect.

[0061] Experimental Example 3 Clinical trial observation on the effect of hydrogen-rich water mask on relieving postpartum chloasma 1. Case selection: A total of 100 cases of melasma diagnosed in the Institute of Dermatology and Venereology of Sichuan Provincial People's Hospital from March 2019 to August 2022 were collected and grouped by random number table extraction. Among the 50 patients in the control group, the age range was 25-40 years old; among the 50 patients in the treatment group, the age range was 22-38 years old. The general information between the groups was balanced and comparable (P>0.05).

[0062] Inclusion criteria: Meet the "Clinical Diagnosis and Treatment Standards for Chloasma (2003 Revised Draft)"; no severe postpartum complications; no lesions of important organs such as the heart, brain, liver, and kidney.

[0063] Exclusion criteria: patients who are allergic to drugs; patients who have received cryotherapy or radiotherapy in the past six months.

[0064] 2. Treatment method: The control group was given hydroquinone cream (20 mg / g) twice a day. The treatment group was given the hydrogen-rich water mask provided in Example 1 of this specification twice a day. Both groups of patients were treated for 60 consecutive days.

[0065] 3. Observation of therapeutic effect Comparison of therapeutic effects: Hydrogen-rich water mask has a better therapeutic effect on melasma, see Table 3.

[0066] Table 3 The therapeutic effect of hydrogen-rich water mask on chloasma Group Number of examples (examples) cure Significant effect efficient invalid Cure rate (%) Treatment Group 50 49 1 0 0 98 Control group 50 25 10 10 5 50 Note: Cured means the color of chloasma disappears, and the area of ​​skin lesions disappears by ≥90%; markedly effective means the color of chloasma becomes significantly lighter, and the area of ​​skin lesions disappears by 60-89%; effective means the color of chloasma becomes lighter, and the area of ​​skin lesions disappears by 30-59%; ineffective means the color of chloasma does not change, and the area of ​​skin lesions disappears by <30%. Total effective rate (%) = (cured + markedly effective + effective) / number of cases * 100%.

[0067] It can be seen from the above experiments that the hydrogen-rich water mask provided by the present invention can effectively alleviate and lighten chloasma and causes little damage to the skin during use.

[0068] In summary, the hydrogen-rich water mask provided by the present invention is used, the essence and the hydrogen generating powder are mixed and then immediately placed on the mask paper for application, and the technology of essence wrapping solid hydrogen generating powder is used to form a hydrogen-rich essence. After the mask paper contacts the hydrogen-rich essence for the first time, a hydrogen-rich mask is generated, which is immediately applied to the face. The hydrogen generating powder releases hydrogen immediately after contacting water, but because it is wrapped in the essence, it can slowly and continuously release hydrogen, thereby better playing the role of anti-aging, anti-inflammatory, freckle removal and skin beautification; A hydrogen-rich water mask, wherein the hydrogen generating powder can release hydrogen after contacting with water, which has significant antioxidant effects, quickly removes harmful active oxygen free radicals and whitens the skin, the American cockroach extract has powerful repair and anti-inflammatory effects, the pomegranate peel extract can improve skin metabolism and can also be used as waste to reduce production costs, and collagen has the effects of firming, wrinkle-smoothing, whitening, and freckles; after forming the hydrogen-rich essence, the effective ingredients of each raw material can complement each other, promote each other to play a synergistic role, thereby further improving the anti-aging, whitening, anti-inflammatory, repair, and freckle removal effects of the hydrogen-rich water mask. The preparation method of the hydrogen-rich water mask provided by the present invention is simple and easy to operate, and can be suitable for industrial production. Therefore, in summary, the experimental data show that the hydrogen-rich water mask provided by the present invention has application prospects in the preparation of products for lightening or removing chloasma.

[0069] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A hydrogen-rich water mask, characterized in that: The raw materials include hydrogen generating powder and essence; the essence includes American cockroach extract, pomegranate peel extract and collagen.

2. The hydrogen-rich water mask according to claim 1, characterized in that: The dosage ratio of the hydrogen generating powder to the essence is 1 mg:8-10 ml.

3. The hydrogen-rich water mask according to claim 2, characterized in that: The hydrogen generating powder comprises magnesium powder and citric acid powder in a mass ratio of 2:9-11.

4. The hydrogen-rich water mask according to claim 2, characterized in that: In parts by weight, the essence comprises: 4-6 parts of the American cockroach extract, 3-5 parts of the pomegranate peel extract and 2-3 parts of the collagen.

5. The hydrogen-rich water facial mask according to claim 4, characterized in that: The preparation method of the American cockroach extract comprises: crushing the inactivated American cockroach into coarse powder, extracting in a supercritical carbon dioxide extraction device, controlling the pressure to 25-28MPa, the temperature to 42-45°C, the carbon dioxide flow rate to 15-18L / h, extracting for 1-1.5h, obtaining and collecting the American cockroach oil to obtain the American cockroach extract.

6. The hydrogen-rich water facial mask according to claim 4, characterized in that: The preparation method of the pomegranate peel extract comprises: collecting pomegranate peel, cleaning it, adding 2 times water to crush it, filtering to obtain a filtrate, adding yeast to ferment the filtrate at 20-22° C. for 6-8 days, collecting the fermented liquid, adding lactic acid bacteria and fermenting it again at a temperature of 15-18° C. for 2-3 days, then ultrasonically extracting at a water temperature of 40-50° C. at normal pressure for 30 minutes to obtain an extract; filtering the extract and concentrating it under reduced pressure to obtain the pomegranate peel extract.

7. The hydrogen-rich water facial mask according to claim 4, characterized in that: In terms of weight, the essence further comprises: 78-80 parts of deionized water, 10-13 parts of glycerin, 0.4-0.6 parts of xanthan gum, 1-2 parts of polysorbate-80, 0.1-0.3 parts of hydroxyethyl cellulose, and 1-2 parts of methylparaben.

8. The hydrogen-rich water facial mask according to claim 7, characterized in that: In terms of weight, the essence further comprises: 4-6 parts of glycerol.

9. A method for preparing the hydrogen-rich water mask as claimed in claim 8, characterized in that: include: (1) adding the American cockroach extract, the pomegranate peel extract, the collagen, the deionized water, the glycerin, the xanthocollagen, the polysorbate-80, the hydroxyethyl cellulose and the methylparaben according to the weight proportions, slowly adding the glycerol dropwise while mixing, and adjusting the pH value to between 6.2 and 6.4 to obtain the essence; (2) The hydrogen generating powder, the essence and the mask paper are packed separately into mask bags, and the part containing the hydrogen generating powder is evacuated.

10. Use of the hydrogen-rich water mask according to any one of claims 1 to 8 in preparing products for lightening or removing chloasma.