Sheet peptizing device and control method

Abstract

The invention discloses a sheet peptizing device and a control method, and the device comprises a device main body which is provided with a plurality of processing bins; the processing chamber is provided with an insertion port, the sample chambers can be inserted into the processing chamber from the insertion port and are in sealed connection with the insertion port, the sample chambers are arranged at least one inner circulation port, and the sample chambers are communicated with the processing chamber through the inner circulation ports; the device main body is further provided with a plurality of heating structures and a plurality of image acquisition structures, the sheet peptizing device further comprises a control mechanism, and the control mechanism is electrically connected with the plurality of heating structures so as to control the plurality of heating structures to heat the treatment liquid in the plurality of treatment cavities; and the control mechanism is electrically connected with the plurality of image acquisition structures so as to control the plurality of image acquisition structures to acquire images of the sample slices in the plurality of sample cavities and analyze the processing states of the sample slices according to the images of the sample slices. Safety risks cannot be caused to external experimenters, the treatment rate and the treatment effect can be improved, and the automation degree of the device is high.

Description

Technical Field

[0001] The invention relates to the technical field of geological exploration research, and in particular to a thin slice debonding device and a control method. Background Art

[0002] Rocks are the direct carriers of various geological processes and the most direct and effective physical materials for studying various geological processes. Making collected rock samples into thin slices for microscopic observation is the most basic research method. Thin slice observation is also indispensable in shale gas exploration research. Since it is difficult to sample rock samples for deep shale gas, usually only very limited existing samples can be studied, which requires improving the reuse rate of rock samples. Thin slice degumming is a means to improve the reuse rate of rock samples. As the name suggests, it is to remove the gum wrapped on the rock slice for easy preservation and restore it to a clean and pure state, so that the rock slice that has been observed can be used as a research sample for other experiments.

[0003] However, after the existing thin-section degumming device degummed the rock thin sections, due to incomplete cleaning, the processed rock thin sections could not meet many advanced experimental requirements, such as the inclusion laser Raman experiment. The Canada gum remaining on the thin sections has fluorescent characteristics, which will have a serious impact on the inclusion laser Raman experiment. Therefore, the thin-section degumming treatment that meets the requirements of advanced experiments is now manually operated, but the degumming liquid used in the degumming process is an organic solvent, which is often highly volatile and weakly toxic, posing an experimental safety risk to the experimenters; and due to the strong volatility of the degumming liquid, in order to avoid experimental accidents, it is often carried out at room temperature, resulting in a lengthy degumming process and slow experimental speed; in addition, the thickness of rock thin sections is generally 0.1mm, which is not easy to preserve. In the manual degumming operation, the thin sections are often broken and the samples are damaged. Summary of the invention

[0004] The purpose of the present invention is to provide a thin slice debonding device and a control method to solve the technical problem that the rock thin slices after debonding treatment by the existing thin slice debonding device cannot meet the requirements and need manual debonding.

[0005] The above-mentioned purpose of the present invention can be achieved by adopting the following technical solutions:

[0006] The present invention provides a thin film degumming device, comprising: a device body, provided with a plurality of processing chambers, the processing chamber having a processing cavity for accommodating a processing liquid; a plurality of sample chambers, the processing chamber being provided with an insertion port, the sample chamber being able to be inserted into the processing cavity from the insertion port and being sealedly connected to the insertion port, the sample chamber having a sample cavity for placing a sample thin film, and the sample chamber being provided at least at one inner flow port, the sample cavity being connected to the processing cavity through the inner flow port; the device body being further provided with a plurality of heating structures and a plurality of image acquisition structures, the thin film degumming device further comprising a control mechanism, the control mechanism being electrically connected to the plurality of heating structures and being able to control the plurality of heating structures to heat the processing liquid in the plurality of processing chambers, the control mechanism being electrically connected to the plurality of image acquisition structures and being able to control the plurality of image acquisition structures to acquire images of the sample thin films in the plurality of sample chambers and analyzing the processing status of the sample thin films according to the images of the sample thin films.

[0007] In an embodiment of the present invention, the thin film degumming device also includes a leveling mechanism, which includes a spirit level and a plurality of lifting support structures. The spirit level is installed on the device body and can detect the inclination angle of the device body relative to the horizontal direction. The plurality of lifting support structures are installed on the device body and can adjust the inclination angle of the device body relative to the horizontal direction by lifting.

[0008] In an embodiment of the present invention, the thin slice disassembly device also includes a plurality of blank glass slides for carrying the sample thin slices, two limiting guide rails are arranged opposite to each other in the horizontal direction in the sample chamber, and the blank glass slide can be slidably inserted between the two limiting guide rails, and the sample chamber is provided with a plurality of thin slice limiting members that can move in the horizontal direction, and the plurality of thin slice limiting members are located above the two limiting guide rails. The plurality of thin slice limiting members can limit the sample thin slice between the thin slice limiting members and the blank glass slide by moving toward the inner side of the two limiting guide rails to above the sample thin slice.

[0009] In an embodiment of the present invention, a temperature detection structure is provided in the processing chamber, and the control mechanism is electrically connected to the temperature detection structure and can control the heating temperature of the heating structure according to a detection signal from the temperature detection structure.

[0010] In an embodiment of the present invention, a plurality of indication structures are installed on the device body, and the plurality of indication structures are arranged one-to-one corresponding to the plurality of sample chambers. The indication structure includes a start indicator light and a stop indicator light. The control mechanism is electrically connected to the indication structure and can control the opening and closing of the start indicator light and the stop indicator light respectively.

[0011] In an embodiment of the present invention, the plurality of heating structures are installed one-to-one under the plurality of processing chambers; the top surface of the sample chamber has a placement opening for placing the sample slice into the sample cavity, and the image acquisition structure is installed in the processing chamber and located above the placement opening.

[0012] In an embodiment of the present invention, the device body is provided with a liquid pipeline structure and at least one liquid inlet and outlet, the processing chamber is provided with at least one external flow outlet, the liquid inlet and outlet are connected to the liquid pipeline structure, and the liquid pipeline structure is respectively connected to the external flow outlet of each processing chamber.

[0013] In an embodiment of the present invention, the treatment liquid is a degumming liquid or a cleaning liquid, and the number of the liquid inlets and outlets is four. The four liquid inlets and outlets are connected to the liquid pipeline structure through a main control valve, wherein two of the liquid inlets and outlets are used for the injection and discharge of the degumming liquid, and the other two of the liquid inlets and outlets are used for the injection and discharge of the cleaning liquid; the number of the outflow outlets of each of the treatment chambers is two, one of the outflow outlets is an external liquid inlet, and the other of the outflow outlet is an external liquid outlet, and the liquid pipeline structure includes a main injection pipeline, a main discharge pipeline, a plurality of branch injection pipelines and a plurality of branch discharge pipelines, the input end of the main injection pipeline and the output end of the main discharge pipeline are respectively connected to the main control valve, the main injection pipeline is respectively connected to the external liquid inlets of the plurality of treatment chambers through the plurality of branch injection pipelines, and each of the branch injection pipelines is provided with a branch control valve, and the input end of the main discharge pipeline is respectively connected to the external liquid inlets of the plurality of treatment chambers through the plurality of branch discharge pipelines.

[0014] The present invention also provides a control method for thin slice degumming, which is used to control the above-mentioned thin slice degumming device, and the control method comprises the following steps: injecting degumming liquid into a processing chamber, so that the degumming liquid flows into a sample chamber to perform degumming treatment on the sample thin slice; wherein the sample thin slice comprises a sample and a colloid wrapped outside the sample; collecting a degumming image of the sample thin slice; obtaining the colloid edge and the sample edge of the degumming image; and judging whether the sample thin slice has completed degumming according to the changes of the colloid edge and the sample edge of the degumming image.

[0015] In an embodiment of the present invention, the control method also includes: before depolymerization, staining the colloid of the sample slice; the depolymerized image is a color image with multiple pixels; the acquisition of the colloid edge and the sample edge of the depolymerized image includes the following steps: processing the color image into a grayscale depolymerized image; determining the grayscale value and coordinates of each pixel of the grayscale depolymerized image; arranging the grayscale values ​​of each pixel in the grayscale depolymerized image according to their coordinates, and determining the low threshold and high threshold of the grayscale value; extracting the coordinates of the pixel points in the grayscale depolymerized image whose grayscale values ​​are greater than the low threshold and less than the high threshold, and recording them as the coordinates of the colloid edge in the grayscale depolymerized image; extracting the coordinates of the pixel points in the grayscale depolymerized image whose grayscale values ​​are greater than or equal to the high threshold, and recording them as the coordinates of the sample edge in the grayscale depolymerized image.

[0016] In an embodiment of the present invention, judging whether the sample slice has completed the dissolution according to the changes of the colloid edge and the sample edge includes the following steps: calculating the difference between the initial coordinates and the current coordinates of the colloid edge, and recording it as the coordinate change value of the colloid edge; calculating the difference between the initial coordinates and the current coordinates of the sample edge, and recording it as the coordinate change value of the sample edge; until the coordinate change value of the colloid edge and the coordinate change value of the sample edge both meet preset conditions, judging that the sample slice has completed the dissolution.

[0017] In an embodiment of the present invention, the control method also includes the following steps: when it is determined that the sample slice has completed degumming, the degumming liquid in the processing chamber is discharged, and the cleaning liquid is injected into the processing chamber, so that the cleaning liquid flows into the sample chamber to clean the sample slice; a cleaning image of the sample slice is collected; the cleaning image is processed into a grayscale cleaning image; the grayscale value and coordinates of each pixel point of the grayscale cleaning image are determined; the coordinates of the pixel points in the grayscale cleaning image whose grayscale values ​​are greater than or equal to the high threshold are extracted, and recorded as the coordinates of the sample edge in the grayscale cleaning image; according to the coordinates of the sample edge in the grayscale cleaning image, a grayscale background image located outside the sample edge in the grayscale cleaning image is obtained; according to the changes in the grayscale background image, whether the sample slice has completed cleaning is determined.

[0018] In an embodiment of the present invention, judging whether the sample slice has been cleaned according to the change of the background image in the cleaning image includes the following steps: determining the grayscale value of each pixel of the grayscale background image; if the grayscale value of each pixel of the grayscale background image is less than or equal to the low threshold, judging that the sample slice has been cleaned.

[0019] The characteristics and advantages of the present invention are:

[0020] The slice degumming device of the present invention installs multiple sample chambers in multiple processing chambers by insertion and seals the insertion ports of the processing chambers, so that the processing liquid in the processing chambers can flow into the sample chambers through the internal flow ports of the sample chambers to perform degumming treatment on the sample slices without causing safety risks to external experimenters, isolating the experimenters from direct contact with the processing liquid, and ensuring the safety of personnel and experiments to the greatest extent.

[0021] The thin-sheet degumming device of the present invention heats the processing liquids in multiple processing chambers by arranging multiple heating structures, thereby facilitating improving the processing rate and processing effect.

[0022] The slice degumming device of the present invention collects images of sample slices in multiple sample chambers by arranging multiple image acquisition structures, so that the control structure can analyze the status of the sample slices according to the images of the sample slices. On the one hand, it can judge whether the degumming is completed according to the images during the degumming process; on the other hand, it can also collect images of the sample slices during the process of cleaning the sample slices after the degumming is completed, and then judge whether the cleaning is completed according to the image changes outside the edge of the sample in the image, thereby improving the degree of automation of the device and avoiding damage to the sample due to frequent manual operation.

[0023] The control method for slice disassembly of the present invention collects the disassembly image of the sample slice during the disassembly process, and then obtains the sample edge and the colloid edge from the disassembly image, so as to judge whether the sample slice has completed disassembly according to the changes of the sample edge and the colloid edge.

[0024] The control method for slice degumming of the present invention collects a cleaning image of the sample slice during the cleaning process after degumming is completed, and then determines whether the sample slice has been cleaned according to the image changes outside the sample edge in the cleaning image. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0026] Figure 1 It is a side view of the internal structure of the device body in the present invention;

[0027] Figure 2 It is a schematic diagram of the installation of the sample slice in the sample chamber of the present invention;

[0028] Figure 3 It is an overall schematic diagram of the thin slice degumming device of the present invention;

[0029] Figure 4 It is a top view of the internal structure of the device body in the present invention;

[0030] Figure 5 is a color image of the sample slice collected in the initial state in the present invention;

[0031] Figure 6 is a grayscale image of the sample slice in the initial state in the present invention;

[0032] Figure 7 is a histogram of grayscale values ​​in the present invention;

[0033] Figure 8 is a grayscale image of the sample slice when debonding is completed in the present invention;

[0034] Fig. 9 It is a grayscale image of the sample slice when the cleaning is completed in the present invention.

[0035] In the figure:

[0036] 1. Device body;

[0037] 11. Processing chamber; 111. Processing chamber; 112. Insertion port; 113. External flow port; 114. External liquid inlet; 115. External liquid outlet; 116. Observation window; 117. Guide groove;

[0038] 12. Heating structure; 13. Image acquisition structure; 14. Temperature detection structure;

[0039] 15. Indication structure; 151. Start indicator light; 152. Stop indicator light;

[0040] 16. Liquid pipeline structure; 161. Main injection pipeline; 162. Main discharge pipeline; 163. Branch injection pipeline; 164. Branch discharge pipeline; 165. Branch control valve;

[0041] 17. Liquid inlet and outlet; 18. Main line control valve;

[0042] 2. Sample compartment; 21. Sample cavity; 22. Internal flow port; 221. Internal liquid inlet; 222. Internal liquid outlet; 23. Limiting guide rail; 24. Thin sheet limiting member; 25. Guide rail; 26. Sealing cover plate; 27. Fixing member; 28. Placement port;

[0043] 3. Leveling mechanism; 31. Level; 32. Lifting support structure; 321. Coarse adjustment roller; 322. Fine adjustment roller; 323. Support screw; 324. Support base;

[0044] 4. Control mechanism; 41. Control module; 42. Power supply interface; 43. Communication interface;

[0045] 5. Blank slide;

[0046] 6. Sample slice; 61. Sample; 62. Colloid; 63. Sample slide;

[0047] 7. Image of sample slice; 71. Sample image; 72. Colloid image; 73. Pixel point. DETAILED DESCRIPTION

[0048] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0049] Implementation Method 1

[0050] like Figure 1 and Figure 2 As shown, the present invention provides a thin film degumming device, comprising: a device body 1, provided with a plurality of processing chambers 11, the processing chamber 11 has a processing chamber 111 for accommodating a processing liquid; a plurality of sample chambers 2, the processing chamber 11 is provided with an insertion port 112, the sample chamber 2 can be inserted into the processing chamber 111 from the insertion port 112 and is sealedly connected to the insertion port 112, the sample chamber 2 has a sample chamber 21 for placing a sample thin film 6, and the sample chamber 2 is provided with at least one internal flow port 22, the sample chamber 21 is connected to the processing chamber 111 through the internal flow port 22; wherein the device body 1 is also provided with a plurality of heating structures 12 and a plurality of image acquisition structures 13, the thin film degumming device also comprises a control mechanism 4, the control mechanism 4 is electrically connected to the plurality of heating structures 12 and can control the plurality of heating structures 12 to heat the processing liquid in the plurality of processing chambers 111, the control mechanism 4 is electrically connected to the plurality of image acquisition structures 13 and can control the plurality of image acquisition structures 13 to acquire images 7 (in the plurality of sample chambers 21) of the sample thin films. Figure 5 ) and determine the processing status of the sample slice 6 based on the image 7 of the sample slice.

[0051] Combination Figure 2 and Figure 3 As shown, the slice degumming device of the present invention installs multiple sample chambers 2 in multiple processing chambers 11 by insertion and seals the insertion ports 112 of the processing chambers 11, so that the processing liquid in the processing chambers 11 can flow into the sample chamber 2 through the internal flow ports 22 of the sample chamber 2 to perform degumming treatment on the sample slices 6 without causing safety risks to external experimenters, thereby isolating the experimenters from direct contact with the processing liquid and ensuring the safety of personnel and experiments to the greatest extent.

[0052] Specific, combined Figure 2As shown, the sample slice 6 includes a sample 61 and a colloid 62 wrapped around the sample 61. In addition, the sample slice 6 also includes a sample glass slide 63, and the sample 61 is supported and fixed on the sample glass slide 63 by the colloid 62. The processing liquid in the processing chamber 11 can be a degumming liquid required for degumming, or it can be a cleaning liquid required for cleaning after the degumming is completed. There are multiple internal flow ports 22 on the sample chamber 2, at least one internal flow port 22 serves as an internal liquid inlet 221 for the processing liquid to flow into the sample chamber 2, and at least one internal flow port 22 serves as an internal liquid outlet 222 for the processing liquid to discharge from the sample chamber 2. In this embodiment, a plurality of internal liquid inlets 221 are provided on one side of the sample chamber 2, and an internal liquid outlet 222 is provided at the bottom of the sample chamber 2. In which, the degumming liquid in the degumming process is relatively statically contained in the sample chamber 2, and in the cleaning process, the cleaning liquid is injected into the sample chamber 2 from the internal liquid inlet 221 at a specific speed, and the degumming liquid is discharged from the internal liquid outlet 222. After the degumming liquid is completely discharged, the cleaning liquid continues to be injected into the sample chamber 2 from the internal liquid inlet 221 at the same speed, and the cleaning liquid in the sample chamber 2 is also discharged from the internal liquid outlet 222, so that the cleaning liquid in the sample chamber 2 remains in a flowing state and is continuously renewed, thereby cleaning the sample slice 6.

[0053] Combination Figure 2 and Figure 3 As shown, the sample bin 2 is generally in a drawer-like structure and is made of corrosion-resistant materials to keep warm and prevent the corrosiveness of the degumming liquid from causing harm to the sample bin 2. One end of the sample bin 2 is inserted into the processing bin 11 from the insertion port 112, and the other end of the sample bin 2 is provided with a sealing cover 26, which is sealed at the insertion port 112 and detachably fixed to the device body 1 through a plurality of fixings 27. Multiple sample bins 2 are arranged in a one-to-one correspondence with multiple processing bins 11, and the number of processing bins 11 and the number of sample bins 2 are not specifically limited, and can be set to six as in the present embodiment, or can be set to other numbers. In addition, in the embodiment of the present invention, in order to facilitate the observation of the processing conditions in the sample bin 2, the sealing cover 26 has a transparent visual area made of a glass plate.

[0054] Combination Figure 1 and Figure 4 As shown, the thin-sheet degumming device of the present invention heats the processing liquid in multiple processing chambers 11 by arranging multiple heating structures 12, thereby facilitating improving the processing rate and processing effect.

[0055] Specifically, the number of heating structures 12 is not specifically limited, and one processing chamber 11 is correspondingly provided with at least one heating structure 12. The heating structure 12 includes, but is not limited to, an electromagnetic heating structure. In some embodiments of the present invention, a plurality of heating structures 12 are installed one-to-one under a plurality of processing chambers 11, so that the heating structure 12 will not contact with the processing liquid and affect its service life. Optionally, the heating structure 12 can also be arranged in the wall of the processing chamber 11 or laid on the outer wall surface of the processing chamber 11. In addition, in order for the control mechanism 4 to better control the heating structure 12 for heating, a temperature detection structure 14 is provided in the processing chamber 11, and the control mechanism 4 is electrically connected to the temperature detection structure 14 and can control the heating temperature of the heating structure 12 according to the detection signal of the temperature detection structure 14.

[0056] Combination Figure 1 and Figure 5 As shown, the slice degumming device of the present invention collects images 7 of sample slices in multiple sample chambers 2 by setting multiple image acquisition structures 13, so that the control mechanism 4 can analyze the status of the sample slice 6 according to the image 7 of the sample slice. On the one hand, it can judge whether the degumming is completed according to the image during the degumming process. On the other hand, it can also collect the image of the sample slice 6 during the process of cleaning the sample slice 6 after the degumming is completed, and then judge whether the cleaning is completed according to the image changes outside the edge of the sample in the image, thereby improving the degree of automation of the device and avoiding damage to the sample 61 caused by frequent manual operations.

[0057] Specifically, the number of image acquisition structures 13 is not specifically limited, and one processing chamber 11 is corresponding to at least one image acquisition structure 13. The image acquisition structure 13 includes but is not limited to an image sensor. The control mechanism 4 includes a control module 41 installed on the device body 1, and the control module 41 is electrically connected to the image acquisition structure 13, the heating structure 12 and the temperature detection structure 14 respectively. In addition, the control mechanism 4 also includes an analysis and processing module and a communication interface 43 and a power interface 42 provided on the device body 1. The control module 41 is electrically connected to the analysis and processing module via the communication interface 43, and the control module 41 is electrically connected to the external power supply via the power interface 42. Figure 1 and Figure 2 As shown, in some embodiments of the present invention, the top surface of the sample chamber 2 has a placement opening 28 for placing the sample slice 6 into the sample cavity 21, and the image acquisition structure 13 is installed in the processing chamber 11 and is located above the placement opening 28. The liquid level of the processing liquid in the processing chamber 11 is lower than the height of the image acquisition structure 13. In the embodiment of the present invention, in order to facilitate the observation of the degumming treatment of the sample slice 6, the top surface of the processing chamber 11 is provided with an observation window 116. Optionally, the image acquisition structure 13 can also be installed above the observation window 116.

[0058] The working process of the thin slice degumming device of the present invention includes: first injecting the degumming liquid into the processing chamber 11, so that the degumming liquid flows into the sample chamber 2 to perform degumming treatment on the sample thin slice 6; collecting an image 7 of the sample thin slice (defined as a degumming image); obtaining the colloid edge and the sample edge of the degumming image; judging whether the sample thin slice 6 has completed degumming according to the changes of the colloid edge and the sample edge of the degumming image.

[0059] Specifically, before depolymerization, the colloid 62 of the sample slice 6 is dyed so that the collected depolymerized image is a color image with multiple pixel points 73, so as to better obtain the colloid edge and sample edge of the depolymerized image, wherein the image 7 of the sample slice includes a sample image 71 and a colloid image 72, the colloid edge is the edge of the colloid image 72, and the sample edge is the edge of the sample image 71; then the color image of the depolymerized image is first processed into a grayscale depolymerized image; then the grayscale value and coordinates of each pixel point 73 of the grayscale depolymerized image are determined; then according to the grayscale value and coordinates of each pixel point 73, the low threshold and high threshold of the grayscale value are determined; the coordinates of the pixel point 73 in the grayscale depolymerized image whose grayscale value is greater than the low threshold and less than the high threshold are extracted, and recorded as the coordinates of the colloid edge of the grayscale depolymerized image; the coordinates of the pixel point 73 in the grayscale depolymerized image whose grayscale value is greater than or equal to the high threshold are extracted, and recorded as the coordinates of the sample edge of the grayscale depolymerized image. Furthermore, the difference between the initial coordinates and the current coordinates of each pixel point 73 at the colloid edge of the grayscale depolymerized image is calculated, and recorded as the coordinate change value of the colloid edge of the grayscale depolymerized image; the difference between the initial coordinates and the current coordinates of each pixel point 73 at the sample edge of the grayscale depolymerized image is calculated, and recorded as the coordinate change value of the sample edge of the grayscale depolymerized image; until the coordinate change value of the colloid edge of the grayscale depolymerized image and the coordinate change value of the sample edge of the grayscale depolymerized image both meet the preset conditions, it is determined that the sample slice 6 has completed depolymerization.

[0060] The gray value c of pixel 73 is: c = R*m r +G*m g +B*m b ; Wherein, R is the red value of the pixel point 73 in the RGB mode, G is the green value of the pixel point 73 in the RGB mode, B is the blue value of the pixel point 73 in the RGB mode, m r is the weighting coefficient corresponding to the red value, m g is the weighting coefficient corresponding to the green value, m bis the weighting coefficient corresponding to the blue value; the coordinates of the pixel point 73 include a horizontal coordinate a and a vertical coordinate b; therefore, the coordinates and grayscale values ​​corresponding to each pixel point 73 are expressed as (a, b, c), and the coordinates and grayscale values ​​corresponding to each pixel point 73 are stored in a matrix and recorded as an image data matrix P; then, by traversing the image data matrix P according to a preset segmentation path, the horizontal coordinate and vertical coordinate of the traversed image data are reduced in dimension and only one is saved, expressed as (a, c) or (b, c), and then the horizontal coordinate or vertical coordinate of each pixel point 73 is arranged and drawn as shown in Figure 7 The histogram of the grayscale values ​​of each pixel point 73 shown in the figure is used to determine the high threshold value k and the low threshold value j; in this embodiment, the preset segmentation paths include the segmentation path A-A' and the segmentation path B-B' arranged along the two diagonals, the segmentation path C-C' arranged along the horizontal midline, and the segmentation path D-D' arranged along the longitudinal midline; finally, the coordinates of the pixel points 73 whose grayscale values ​​are greater than the low threshold value j and less than the high threshold value k are extracted and recorded as the coordinates of the colloid edge; the coordinates of the pixel points 73 whose grayscale values ​​are greater than or equal to the high threshold value k are extracted and recorded as the coordinates of the sample edge; wherein, when the gel dissolution starts, the initial coordinates of each pixel point 73 at the edge of the colloid are stored in a matrix and recorded as the coordinates of the colloid edge. Coordinate data matrix E0, the initial coordinates of each pixel point 73 at the edge of the sample are stored in a matrix and recorded as the coordinate data matrix F0 of the edge of the sample; during the degumming process, the current coordinates of each pixel point 73 at the edge of the colloid are stored in a matrix and recorded as the coordinate data matrix E of the edge of the colloid, and the current coordinates of each pixel point 73 at the edge of the sample are stored in a matrix and recorded as the coordinate data matrix F of the edge of the sample; therefore, the coordinate change value ΔE of the edge of the colloid is E-E0, and the coordinate change value ΔF of the edge of the sample is F-F0; thereby, by monitoring whether the coordinate change value ΔE of the edge of the colloid and the coordinate change value ΔF of the edge of the sample meet the preset conditions, it can be determined whether the sample sheet 6 has completed degumming.

[0061] Combination Figure 1 , Figure 8 and Fig. 9As shown, the working process of the slice degumming device of the present invention also includes: when it is determined that the sample slice 6 has completed degumming, the degumming liquid in the processing chamber 11 is discharged, and the cleaning liquid is injected into the processing chamber 11, so that the cleaning liquid flows into the sample chamber 2 to clean the sample 61; the image 7 of the sample slice 6 is collected (defined as the cleaning image); the cleaning image is processed into a grayscale cleaning image; the grayscale value and coordinates of each pixel point of the grayscale cleaning image are determined; the coordinates of the pixel points whose grayscale value is greater than or equal to the high threshold in the grayscale cleaning image are extracted, and recorded as the coordinates of the sample edge in the grayscale cleaning image, that is, the coordinates of the sample edge in the grayscale cleaning image, which are the same as the above-mentioned degumming control The working principle involved in obtaining the sample edge in the grayscale degummed image during the process is the same, so the high threshold k determined in the degumming process can be used to determine the coordinates of the sample edge in the grayscale cleaned image. Since the colloid is dissolved and broken by the degumming liquid, there is no need to obtain the colloid edge in the grayscale cleaned image during the cleaning process; according to the coordinates of the sample edge in the grayscale cleaned image, the grayscale background image located outside the sample edge in the grayscale cleaned image is obtained; the grayscale value of each pixel point of the grayscale background image is determined; if the grayscale value of each pixel point of the grayscale background image is less than or equal to the low threshold j, it is judged that the sample slice 6 has completed cleaning.

[0062] Combination Figure 5 and Figure 6 As shown, after the sample slice 6 is debonded, the colloid 62 is dissolved and broken by the debonding liquid, and a part of the dissolved and broken colloid 62 is carried away by the debonding liquid, while another part adheres to the surface of the sample slide 63. Therefore, when this part of the dissolved colloid 62 is removed by the flow of the cleaning liquid, the grayscale of the image outside the sample 61 can be changed, so that it can be judged whether the sample slice 6 has been cleaned according to the change of the grayscale background image.

[0063] Combination Figure 1 and Figure 3 As shown, in order for the experimenter to know the dissolution process in time, in the embodiment of the present invention, a plurality of indicating structures 15 are installed on the device body 1, and the plurality of indicating structures 15 are arranged one by one corresponding to the plurality of sample chambers 2. The indicating structure 15 includes a start indicator light 151 and a stop indicator light 152. The control mechanism 4 is electrically connected to the indicating structure 15 and can control the start indicator light 151 and the stop indicator light 152 to turn on and off respectively. During the process from the start of dissolution to the completion of cleaning, the start indicator light 151 turns on and the stop indicator light 152 turns off; after the cleaning is completed, the start indicator light 151 turns off and the stop indicator light 152 turns on, thereby reminding the experimenter not to take out the sample chamber 2 during the process from the start of dissolution to the completion of cleaning, and to take out the sample chamber 2 after the cleaning is completed to take out the dissolution sample slice 6.

[0064] Combination Figure 1 and Figure 3As shown, in order to ensure that the whole sample slice 6 can be always immersed in the disintegrating liquid, it is necessary to ensure that the whole device body 1 is in a horizontal state. In the embodiment of the present invention, the slice disintegrating device also includes a leveling mechanism 3, which includes a level 31 and a plurality of lifting support structures 32. The level 31 is installed on the device body 1 and can detect the inclination angle of the device body 1 relative to the horizontal direction. The plurality of lifting support structures 32 are installed on the device body 1 and can adjust the inclination angle of the device body 1 relative to the horizontal direction by lifting. Specifically, the lifting support structure 32 includes a support screw 323, a support base 324 and a lifting adjustment roller. The device body 1 is provided with a mounting groove for mounting the lifting adjustment roller. The upper and lower ends of the lifting adjustment roller are in contact with the top and bottom surfaces of the mounting groove. The support screw 323 is penetrated on the device body 1 and is threadedly connected with the lifting adjustment roller. By rotating the lifting adjustment roller to adjust its height on the support screw 323, the device body 1 can be driven to move up and down. The bottom end of the support screw 323 is installed with a base.

[0065] Among them, the lifting adjustment roller includes a coarse adjustment roller 321 and a fine adjustment roller 322. The lifting screw has a coarse thread section that is threadedly matched with the coarse adjustment roller 321 and a fine thread section that is threadedly matched with the fine adjustment roller 322. The pitch of the coarse thread section is greater than the pitch of the fine thread section. Therefore, the adjustment amount of the device body 1 driven to rise and fall by one circle of the coarse adjustment roller 321 is greater than the adjustment amount of the device body 1 driven to rise and fall by one circle of the fine adjustment roller 322. The outer diameter of the coarse adjustment roller 321 is greater than the outer diameter of the fine adjustment roller 322 for easy identification. The number and arrangement of the lifting support structures 32 are not specifically limited. In this embodiment, the device body 1 is generally a rectangular parallelepiped structure, and four lifting support structures 32 are installed at the four corners of the device body 1.

[0066] like Figure 2As shown, in order to prevent the sample slice 6 from moving in the sample chamber 2 and affecting the image recognition result and the degumming effect, in an embodiment of the present invention, the slice degumming device also includes a plurality of blank glass slides 5 for carrying the sample slice 6, and two limiting guide rails 23 are relatively arranged in the horizontal direction in the sample chamber 2, and the blank glass slide 5 can be slidably inserted between the two limiting guide rails 23, and the sample chamber 2 is provided with a plurality of slice limiting members 24 that can move in the horizontal direction, and the plurality of slice limiting members 24 are located above the two limiting guide rails 23, and the plurality of slice limiting members 24 can limit the sample slice 6 between the slice limiting members 24 and the blank glass slide 5 by moving toward the inner side of the two guide rails 25 to above the sample slice 6. During installation, the blank glass slide 5 is first slidably inserted between the two limiting guide rails 23, and then the sample slice 6 is placed on the blank glass slide 5, and then the slice limiting member 24 is adjusted to move horizontally above the sample slice 6; after the debonding is completed, the sample slice 6 uses gravity and buoyancy to make the debonded sample slice 6 automatically, slowly and smoothly fall onto the blank glass slide 5, thereby effectively avoiding damage to the sample 61 on the sample slice 6; during disassembly, the blank glass slide 5 carrying the sample slice 6 is pulled out from the two limiting guide rails 23. Among them, the slice limiting member 24 can be a bolt as in the present embodiment.

[0067] In addition, if Figure 2 and Figure 4 As shown, in order to guide the sample chamber 2 to be inserted into the processing chamber 11 in the horizontal direction and to be installed stably, the sample chamber 2 has two outer side surfaces arranged opposite to each other in the horizontal direction, and the processing chamber 11 has two inner side surfaces arranged opposite to each other in the horizontal direction; two sliding matching structures are arranged between the two inner side surfaces of the sample chamber 2 and the two inner side surfaces of the processing chamber 11; the sliding matching structure includes a guide rail 25 and a guide groove 117, one of which is arranged on the outer side surface of the sample chamber 2, and the other is arranged on the inner side surface of the processing chamber 11. In this embodiment, the two guide rails 25 are arranged on the two outer side surfaces of the sample chamber 2, and the two guide grooves 117 are arranged on the two inner side surfaces of the processing chamber 11.

[0068] like Figure 3 and Figure 4 As shown, in order to further improve the safety of the experiment, in the embodiment of the present invention, the device body 1 is provided with a liquid pipeline structure 16 and at least one liquid inlet and outlet 17, and the processing chamber 11 is provided with at least one external flow opening 113, and the liquid inlet and outlet 17 is connected to the liquid pipeline structure 16, and the liquid pipeline structure 16 is respectively connected to the external flow opening 113 of each processing chamber 11. By connecting the liquid inlet and outlet 17 to the corresponding external pipeline, the injection and discharge of the processing liquid can be realized without opening the processing chamber 11, thereby improving the safety of the experiment and facilitating the operation.

[0069] Specifically, the number of liquid inlets and outlets 17 is four, and the four liquid inlets and outlets 17 are connected to the liquid pipeline structure 16 through the main line control valve 18, wherein two liquid inlets and outlets 17 are used for the injection and discharge of the degumming liquid, and the other two liquid inlets and outlets 17 are used for the injection and discharge of the cleaning liquid; the number of external flow ports 113 of each processing chamber 11 is two, one external flow port 113 is an external liquid inlet 114, and the other external flow port 113 is an external liquid outlet 115, and the liquid pipeline structure 16 includes a main injection pipeline 161, a main discharge pipeline 162, and a main discharge pipeline 163. , multiple branch injection pipelines 163 and multiple branch discharge pipelines 164, the input end of the main injection pipeline 161 and the output end of the main discharge pipeline 162 are respectively connected to the main control valve 18, the main injection pipeline 161 is respectively connected to the external liquid inlet 114 of multiple processing chambers 11 through multiple branch injection pipelines 163, and each branch injection pipeline 163 is provided with a branch control valve 165, and the input end of the main discharge pipeline 162 is respectively connected to the external liquid outlet 115 of multiple processing chambers 11 through multiple branch discharge pipelines 164. Among them, the main control valve 18 and the branch control valve 165 are both solenoid valves, and the main control valve 18 and each branch control valve 165 are respectively electrically connected to the control mechanism 4, and the control mechanism 4 controls the opening and closing of the main control valve 18 and each branch control valve 165 according to the degumming process.

[0070] Implementation Method 2

[0071] Combination Figures 1 to 4 As shown, the present invention also provides a control method for thin slice disintegration, which is used to control the thin slice disintegration device. The thin slice disintegration device in this embodiment has the same specific structure, working principle and beneficial effects as the thin slice disintegration device in embodiment 1. The control method in this embodiment can be implemented with reference to the thin slice disintegration device in embodiment 1, and will not be repeated here.

[0072] Combination Figures 5 to 8 As shown, the control method of the present invention includes the following steps: injecting a degumming liquid into the processing chamber 11, so that the degumming liquid flows into the sample chamber 2 to perform a degumming treatment on the sample slice 6; wherein the sample slice 6 includes a sample 61 and a colloid 62 wrapped outside the sample 61; collecting a degumming image of the sample slice 6; obtaining the colloid edge and the sample edge of the degumming image; judging whether the sample slice 6 has completed degumming according to the changes of the colloid edge and the sample edge of the degumming image.

[0073] In an embodiment of the present invention, the control method further includes: before degumming, dyeing the colloid 62 of the sample slice 6; the degummed image is a color image with multiple pixel points 73; obtaining the colloid edge and sample edge of the degummed image, including the following steps: processing the color image into a grayscale degummed image; determining the grayscale value and coordinates of each pixel point 73 of the grayscale degummed image; arranging the grayscale values ​​of each pixel point 73 of the grayscale degummed image according to their coordinates, and determining the low threshold and high threshold of the grayscale value; extracting the coordinates of the pixel point 73 in the grayscale degummed image whose grayscale value is greater than the low threshold and less than the high threshold, and recording them as the coordinates of the colloid edge of the grayscale degummed image; extracting the coordinates of the pixel point 73 in the grayscale degummed image whose grayscale value is greater than or equal to the high threshold, and recording them as the coordinates of the sample edge of the grayscale degummed image;

[0074] In an embodiment of the present invention, whether the sample slice 6 has completed the degumming is determined based on the changes in the colloid edge and the sample edge of the degumming image; the following steps are included: calculating the difference between the initial coordinates and the current coordinates of the colloid edge, and recording it as the coordinate change value of the colloid edge; calculating the difference between the initial coordinates and the current coordinates of the sample edge, and recording it as the coordinate change value of the sample edge; until the coordinate change value of the colloid edge and the coordinate change value of the sample edge both meet the preset conditions, it is determined that the sample slice 6 has completed the degumming.

[0075] Combination Figure 8 and Fig. 9 As shown, in an embodiment of the present invention, the control method further includes the following steps: when it is determined that the sample slice 6 has completed degumming, the degumming liquid in the processing chamber 11 is discharged, and the cleaning liquid is injected into the processing chamber 11, so that the cleaning liquid flows into the sample chamber 2 to clean the sample 61; a cleaning image of the sample slice 6 is collected; the cleaning image is processed into a grayscale cleaning image; the grayscale value and coordinates of each pixel point of the grayscale cleaning image are determined; the coordinates of the pixel points whose grayscale values ​​are greater than or equal to the high threshold in the grayscale cleaning image are extracted, and recorded as the coordinates of the sample edge in the grayscale cleaning image, which is the same as the working principle involved in obtaining the sample edge in the grayscale degumming image in the above-mentioned degumming control process. Therefore, the high threshold determined in the degumming control process can be used to determine the coordinates of the sample edge in the grayscale cleaning image. Since the colloid is dissolved and broken by the degumming liquid, there is no need to obtain the colloid edge in the grayscale cleaning image during the cleaning process; according to the coordinates of the sample edge in the grayscale cleaning image, a grayscale background image located outside the sample edge in the grayscale cleaning image is obtained; according to the change of the grayscale background image, whether the sample slice 6 has completed cleaning is determined.

[0076] In the embodiment of the present invention, judging whether the sample slice has been cleaned according to the change of the background image in the cleaning image includes the following steps: determining the grayscale value of each pixel of the grayscale background image; if the grayscale value of each pixel of the grayscale background image is less than or equal to the low threshold, judging that the sample slice 6 has been cleaned. That is to say, in the cleaning control process, the low threshold determined in the dissolution control process can be used to analyze whether there is a broken colloid image in the grayscale background image. When the grayscale value of each pixel of the grayscale background image is less than or equal to the low threshold, it means that there is no broken colloid image in the grayscale background image, and therefore, the colloid on the sample slice 6 is cleaned and the sample slice 6 has been cleaned.

[0077] The above are only several embodiments of the present invention. Those skilled in the art may make various changes or modifications to the embodiments of the present invention based on the contents disclosed in the application documents without departing from the spirit and scope of the present invention.

Claims

1. A thin film disintegration device, characterized in that: include: The device body is provided with a plurality of processing chambers, each of which has a processing cavity for containing a processing liquid; A plurality of sample chambers, wherein the processing chamber is provided with an insertion port, the sample chamber can be inserted into the processing chamber from the insertion port and is sealedly connected to the insertion port, the sample chamber has a sample cavity for placing a sample slice, and the sample chamber is provided with at least one inner flow port, and the sample cavity is connected to the processing chamber through the inner flow port; In which, the device body is also provided with a plurality of heating structures and a plurality of image acquisition structures, and the thin film disintegration device also includes a control mechanism, which is electrically connected to the plurality of heating structures and can control the plurality of heating structures to heat the processing liquids in the plurality of processing chambers, and the control mechanism is electrically connected to the plurality of image acquisition structures and can control the plurality of image acquisition structures to acquire images of the sample slices in the plurality of sample chambers and analyze the processing status of the sample slices according to the images of the sample slices.

2. The thin film disintegration device according to claim 1, characterized in that: The thin film degumming device also includes a leveling mechanism, which includes a spirit level and multiple lifting support structures. The spirit level is installed on the device body and can detect the inclination angle of the device body relative to the horizontal direction. The multiple lifting support structures are installed on the device body and can adjust the inclination angle of the device body relative to the horizontal direction by lifting.

3. The thin film disintegration device according to claim 1, characterized in that: The thin slice degumming device also includes a plurality of blank glass slides for carrying the sample thin slices. Two limiting guide rails are arranged opposite to each other in the horizontal direction in the sample chamber. The blank glass slide can be slidably inserted between the two limiting guide rails. The sample chamber is provided with a plurality of thin slice limiting members that can move in the horizontal direction. The plurality of thin slice limiting members are located above the two limiting guide rails. The plurality of thin slice limiting members can limit the sample thin slice between the thin slice limiting members and the blank glass slide by moving toward the inner side of the two limiting guide rails to above the sample thin slice.

4. The thin film disintegration device according to claim 1, characterized in that: A temperature detection structure is provided in the processing chamber, and the control mechanism is electrically connected to the temperature detection structure and can control the heating temperature of the heating structure according to a detection signal of the temperature detection structure.

5. The thin film disintegration device according to claim 1, characterized in that: A plurality of indication structures are installed on the device body, and the plurality of indication structures are arranged one-to-one corresponding to the plurality of sample chambers. The indication structures include a start indicator light and a stop indicator light. The control mechanism is electrically connected to the indication structure and can control the opening and closing of the start indicator light and the stop indicator light respectively.

6. The thin film disintegration device according to claim 1, characterized in that: The plurality of heating structures are installed one by one under the plurality of processing chambers; the top surface of the sample chamber has a placement opening for placing the sample slice into the sample cavity, and the image acquisition structure is installed in the processing chamber and located above the placement opening.

7. The thin film disintegration device according to claim 1, characterized in that: The device body is provided with a liquid pipeline structure and at least one liquid inlet and outlet, the processing chamber is provided with at least one external flow outlet, the liquid inlet and outlet are connected to the liquid pipeline structure, and the liquid pipeline structure is respectively connected to the external flow outlet of each processing chamber.

8. The thin film disintegration device according to claim 7, characterized in that: The treatment liquid is a dissolving liquid or a cleaning liquid, and the number of the liquid inlets and outlets is four, and the four liquid inlets and outlets are connected to the liquid pipeline structure through a main line control valve, two of which are used for the injection and discharge of the dissolving liquid, and the other two are used for the injection and discharge of the cleaning liquid; The number of the outflow outlets of each of the processing chambers is two, one of the outflow outlets is an external liquid inlet, and the other of the outflow outlets is an external liquid outlet. The liquid pipeline structure includes a main injection pipeline, a main discharge pipeline, multiple branch injection pipelines and multiple branch discharge pipelines. The input end of the main injection pipeline and the output end of the main discharge pipeline are respectively connected to the main control valve, the main injection pipeline is respectively connected to the external liquid inlets of the multiple processing chambers through the multiple branch injection pipelines, and each of the branch injection pipelines is provided with a branch control valve, and the input end of the main discharge pipeline is respectively connected to the external liquid outlets of the multiple processing chambers through the multiple branch discharge pipelines.

9. A method for controlling the disintegration of a thin film, characterized in that: Used to control the thin slice disintegration device according to any one of claims 1 to 8, the control method comprises the following steps: Injecting a degumming liquid into the processing chamber, so that the degumming liquid flows into the sample chamber to perform degumming treatment on the sample slice; wherein the sample slice includes a sample and a colloid wrapped outside the sample; Collecting a degumming image of the sample slice; Acquiring the colloid edge and the sample edge of the degelatinization image; Whether the sample slice has been debonded is determined based on changes in the colloid edge and the sample edge of the debonding image.

10. The control method according to claim 9, characterized in that: The control method further comprises: before degumming, dyeing the colloid of the sample slice; the degummed image is a color image with a plurality of pixel points; The step of obtaining the colloid edge and the sample edge of the decolloidation image comprises the following steps: Processing the color image into a grayscale depolymerized image; Determine the grayscale value and coordinates of each pixel of the grayscale depolymerized image; Arrange the grayscale values ​​of each pixel in the grayscale depolymerized image according to their coordinates, and determine a low threshold and a high threshold of the grayscale value; Extracting the coordinates of the pixel points whose grayscale values ​​in the grayscale depolymerized image are greater than the low threshold and less than the high threshold, and recording them as the coordinates of the colloid edge in the grayscale depolymerized image; The coordinates of the pixel points in the grayscale depolymerized image whose grayscale values ​​are greater than or equal to the high threshold are extracted and recorded as the coordinates of the sample edge in the grayscale depolymerized image.

11. The control method according to claim 10, characterized in that: The method of judging whether the sample slice has been debonded according to the changes of the colloid edge and the sample edge comprises the following steps: Calculating the difference between the initial coordinates and the current coordinates of the colloid edge, and recording it as the coordinate change value of the colloid edge; Calculating the difference between the initial coordinates and the current coordinates of the edge of the sample, and recording it as the coordinate change value of the edge of the sample; When the coordinate change value of the edge of the colloid and the coordinate change value of the edge of the sample meet the preset conditions, it is determined that the sample slice has completed the degumming.

12. The control method according to claim 10, characterized in that: The control method further comprises the following steps: When it is determined that the sample slice has been debonded, the debonding liquid in the processing chamber is discharged, and a cleaning liquid is injected into the processing chamber, so that the cleaning liquid flows into the sample chamber to clean the sample slice; collecting a cleaning image of the sample slice; Processing the cleaned image into a grayscale cleaned image; Determine the grayscale value and coordinates of each pixel of the grayscale cleaned image; Extracting the coordinates of the pixel points whose grayscale values ​​are greater than or equal to the high threshold in the grayscale cleaned image, and recording them as the coordinates of the sample edge in the grayscale cleaned image; According to the coordinates of the sample edge in the grayscale cleaned image, a grayscale background image located outside the sample edge in the grayscale cleaned image is acquired; Whether the sample slice has been cleaned is determined based on the change of the gray background image.

13. The control method according to claim 12, characterized in that: The step of judging whether the sample slice has been cleaned according to the change of the background image in the cleaning image comprises the following steps: Determine the grayscale value of each pixel of the grayscale background image; If the grayscale value of each pixel point of the grayscale background image is less than or equal to the lower threshold, it is determined that the sample slice has been cleaned.

Images