Application of sodium butyrate in improving quality of queen bee

Abstract

The invention discloses application of sodium butyrate in improving quality of queen bees, and belongs to the technical field of biology and agriculture. According to the method, starting from the direct environment of queen bee development, namely the feeding group, the intestinal mucosa nutrient sodium butyrate is added into the feed of the bee group, so that the nutrition management of the feeding group is optimized, the nutrition level of the feeding group is improved, and the feed utilization rate of the feeding bee is improved, thereby providing higher-quality nutrient substances for the development of the queen bee; the quantity and quality of royal jelly secreted by feeding bees are increased, so that the purpose of improving the quality of queen bees is achieved. In addition, the sodium butyrate is good in safety when being used for feeding swarms, and does not generate negative effects on the queen cell acceptance rate and the house output rate.

Description

Technical Field

[0001] The invention belongs to the field of biology and agricultural technology, and in particular relates to application of sodium butyrate in improving the quality of queen bees. Background Art

[0002] The queen of the Chinese honey bee is a fully developed female bee in the bee colony, and its main function is to lay eggs. As an important member of the bee colony, the quality of the queen plays an important role in the development and production performance of the bee colony. The breeding of Chinese honey bees has extensive economic and ecological benefits. It provides humans with high-quality natural foods such as honey and pollen, provides pollination services for many crops, effectively increases their yields, and also plays an important role in maintaining ecological balance. With the improvement of people's health awareness, the demand for bee products such as honey and pollen is increasing, and there are higher requirements for the quality of bee colonies, the yield and quality of bee products in the process of bee breeding. In the beekeeping industry, there are sayings such as "a good queen is worth ten thousand pounds of honey" and "beekeeping is raising queens", which well reflects the importance of high-quality queens in the process of bee breeding. Although the quality of queens has been improved to a certain extent through the application of technical means such as population selection, queen confinement, egg transfer, control of worm transfer age, and queen breeding without worm transfer in the study of artificial queen breeding, the above studies are all based on genetic factors and the individual development of queens. It is difficult to further improve the quality of queens by improving the above technologies.

[0003] Therefore, how to further improve the quality of queen bees has become a technical problem that urgently needs to be solved in this field. Summary of the invention

[0004] In order to solve the above technical problems, the present invention proposes the application of sodium butyrate in improving the quality of queen bees.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] The invention provides application of sodium butyrate in improving the quality of queen bees.

[0007] Preferably, the application specifically comprises the following steps: mixing sodium butyrate and sucrose solution to obtain a sodium butyrate-sucrose solution; and feeding the bee colony with the obtained sodium butyrate-sucrose solution.

[0008] Preferably, the concentration of sodium butyrate in the sodium butyrate-sucrose solution is 10-40 mM / L.

[0009] Preferably, the concentration of sodium butyrate in the sodium butyrate-sucrose solution is 40 mM / L.

[0010] Preferably, the feeding amount is 300-500 mL per group per day.

[0011] Preferably, the timing of starting feeding is 7 to 10 days before queen rearing.

[0012] Preferably, the feeding is terminated 4 to 5 days after the worms are transferred.

[0013] Preferably, the bee colony is a breeding colony.

[0014] Preferably, the preparation method of the sodium butyrate-sucrose solution is specifically as follows: firstly dissolving sodium butyrate with ultrapure water, and then adding sucrose syrup to make up the volume to obtain the sodium butyrate-sucrose solution.

[0015] Compared with the prior art, the present invention has the following advantages and technical effects:

[0016] The invention can improve the queen bee's birth weight, body length, chest width, chest length and other indicators by feeding a certain concentration of sodium butyrate to the nursing colony during the artificial queen rearing process, indicating that feeding sodium butyrate to the bee colony during the artificial queen rearing process can effectively improve the queen bee quality. In addition, sodium butyrate feeding the bee colony has good safety and will not have a negative impact on the queen cell acceptance rate and the cell-emergence rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The drawings constituting a part of the present application are used to provide a further understanding of the present application. The illustrative embodiments and descriptions of the present application are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0018] Figure 1 The effect of sodium butyrate concentration on the birth weight of queen bees in the present invention;

[0019] Figure 2 This is the effect of sodium butyrate concentration on queen cell acceptance rate and birth rate in the present invention. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0022] The embodiment of the present invention provides the application of sodium butyrate in improving the quality of queen bees. The present invention starts from the direct environment of queen bee development, the nursery colony, and selects to add intestinal mucosal nutrient sodium butyrate to the bee colony feed, optimizes the nutritional management of the nursery colony, improves the nutritional level of the nursery colony, and improves the feed utilization rate of the nursery bees, thereby providing better quality nutrients for the development of queen bees, increasing the quantity and quality of royal jelly secreted by the nursery bees, so as to achieve the purpose of improving the quality of queen bees.

[0023] The present invention can improve the queen bee's birth weight, body length, chest width, chest length and other indicators by feeding the bee colony with sodium butyrate. The queen bee's birth weight is positively correlated with the number of ovarian tubes of the queen bee. The greater the queen bee's birth weight, the stronger the queen bee's reproductive ability. The queen bee's body length, chest width, chest length and other indicators represent the queen bee's body development status. The queen bee with good body development status may have strong production performance, indicating that feeding the bee colony with sodium butyrate during the artificial queen breeding process can effectively improve the queen bee's quality. In addition, sodium butyrate feeding the bee colony has good safety and will not have a negative impact on the queen cell acceptance rate and the rate of leaving the cell.

[0024] In a preferred embodiment, the application specifically comprises the following steps: mixing sodium butyrate and sucrose solution to obtain sodium butyrate-sucrose solution; and feeding bee colonies with the obtained sodium butyrate-sucrose solution. The present invention first mixes sodium butyrate and sucrose solution to prepare a sodium butyrate-sucrose solution of a certain concentration, and then feeds the bee colonies with the obtained sodium butyrate-sucrose solution, thereby effectively improving the quality of queen bees.

[0025] In a preferred embodiment, the concentration of sodium butyrate in the sodium butyrate-sucrose solution is 10-40 mM / L, more preferably 40 mM / L. When the concentration of sodium butyrate in the present invention is too low or too high, the queen bee body length, chest width, chest length and other indicators do not increase significantly, and the queen bee quality cannot be effectively improved.

[0026] In a preferred embodiment, the feeding amount is 300-500 mL per colony per day, the feeding time is evening every day, and the number of bees in each colony is about 16,000.

[0027] In a preferred embodiment, the timing of starting feeding is 7 to 10 days before queen rearing.

[0028] In a preferred embodiment, the feeding is terminated 4 to 5 days after worm transfer.

[0029] In a preferred embodiment, the bee colony is a breeding colony; the bee species of the bee colony is Apis cerana cerana; and the colony strength of the bee colony is 4 combs.

[0030] In a preferred embodiment, the preparation method of the sodium butyrate-sucrose solution is specifically as follows: sodium butyrate is first dissolved in ultrapure water, and then sucrose syrup is added to make up the volume to obtain the sodium butyrate-sucrose solution; the mass ratio of sucrose to water in the sucrose syrup is 1:1. The sodium butyrate-sucrose solution in the present invention is prepared and used before each use.

[0031] In the following examples, the Chinese honey bees were raised in the experimental bee farm of the Guizhou Modern Agricultural Development Institute;

[0032] Sucrose was purchased from Guangxi Nanning Mingyang Sugar Co., Ltd.; sodium butyrate powder (NaB) was purchased from Beijing Solebow Technology Co., Ltd., Cat#S9491, CAS: 156-54-7, molecular weight 110.09 g / mol.

[0033] Examples 1-3 and Comparative Examples 1-2

[0034] (1) Preparation of bee colonies: Fifteen colonies with 4 combs and no pests and diseases were selected as the breeding colonies and randomly divided into five groups, labeled as CK, 10NaB, 20NaB, 40NaB, and 80NaB, with three colonies in each group and about 16,000 bees in each colony. In addition, one colony with uniform color, strong colony strength, and no pests and diseases was selected as the breeding colony to provide small larvae for queen rearing. The small larvae used for queen rearing in all experimental groups came from the same colony.

[0035] (2) Preparation of sodium butyrate-sucrose solution: sucrose and water were dissolved in a mass ratio of 1:1 and boiled and cooled before use to obtain sucrose syrup; 0 g, 1.1 g, 2.2 g, 4.4 g, and 8.8 g of sodium butyrate powder were weighed and put into mineral water bottles marked as CK, 10 NaB, 20 NaB, 40 NaB, and 80 NaB, respectively, 20 mL of ultrapure water was added to each mineral water bottle to fully dissolve the sodium butyrate powder, and then sucrose syrup was added to make the volume to 1000 mL, and the mixture was fully mixed to obtain sodium butyrate-sucrose solutions with sodium butyrate concentrations of 0 mM / L (CK group, comparative example 1), 10 mM / L (10 NaB group, example 1), 20 mM / L (20 NaB group, example 2), 40 mM / L (40 NaB group, example 3), and 80 mM / L (80 NaB group, comparative example 2).

[0036] (3) Feeding the nursing colonies with sodium butyrate-sucrose solution: Starting 7 days before queen rearing, feed the 5 nursing colonies in step (1). Feed the 5 nursing colonies with sodium butyrate-sucrose solution of corresponding concentration every evening, 333 mL per colony per day, until 4 days after queen rearing (queen cells are capped).

[0037] (4) Transferring and rearing queen bees: 5 days before transferring and rearing queen bees, select an empty comb and place it in the queen limiter. Place it in the colony cleaning process in step (1) for 4 to 8 hours. In the evening of the same day, move the queen bee to the queen limiter. Allow the queen bee to lay eggs on the designated comb for 12 hours before removing the queen bee. After 96 hours, transfer queen bees to rear queen bees. The transferred worms are 1 to 1.5 days old. 30 worms are transferred from each experimental colony.

[0038] 10 days after the transfer, the queen cells were transferred to the artificial climate box. The queen bees were checked every 20 minutes for their emergence. The birth weight, body length, chest width, chest length, and queen cell length of the queens that emerged were measured and recorded in time. After all the queen bees emerged, the queen emergence rate was calculated. The results are shown in the table below. Figure 1-2 and Table 1.

[0039] Effect of sodium butyrate concentration on the birth weight of queen bees Figure 1 , a, b, c in the figure represent the significance level. Figure 1 It can be seen that feeding the nursing colony with sodium butyrate-sucrose solution with sodium butyrate concentrations of 10mM / L, 20mM / L, 40mM / L, and 80mM / L can increase the birth weight of the queen. The birth weight of the queen in the experimental group with sodium butyrate concentrations of 20mM / L and 40mM / L increased significantly, and the best effect was achieved when the sodium butyrate concentration was 40mM / L.

[0040] The effects of sodium butyrate concentration on queen cell length, queen body length, queen chest width and queen chest length are shown in Table 1.

[0041] Table 1 Effect of sodium butyrate concentration on queen cell length, queen body length, queen chest width and queen chest length

[0042]

[0043] In the table, a, b, and c represent the significance levels.

[0044] As can be seen from Table 1, when the nursing colony was fed with sodium butyrate-sucrose solution of different concentrations, the length of the queen cell did not show regular changes; the queen body length, chest width, chest length and other indicators increased to varying degrees. The queen body length of the experimental group with a sodium butyrate concentration of 20mM / L increased significantly, and the queen chest width and chest length of the experimental group with a sodium butyrate concentration of 40mM / L increased significantly.

[0045] The effect of sodium butyrate concentration on queen cell acceptance and eviction rate is shown in Figure 2 .Depend on Figure 2 It can be seen that when the nursing groups were fed with sodium butyrate-sucrose solution of different concentrations, there were no significant differences in the queen cell acceptance rate and the queen cell emergence rate among the groups, and there was no negative impact on queen breeding.

[0046] In summary, feeding a certain concentration of sodium butyrate to the nursing colony during the process of artificial queen rearing can effectively improve the quality of queen bees. The sodium butyrate concentration of 40mM / L has the most significant effect, and sodium butyrate feeding bee colonies is safe. The experimental bee species of this method is Apis cerana cerana, and it can be extended to Apis cerana cerana and other bee species.

[0047] The above are only preferred specific implementations of the present application, but the protection scope of the present application is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed in the present application should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.

Claims

1. Application of sodium butyrate in improving the quality of queen bees.

2. The use of sodium butyrate in improving the quality of queen bees according to claim 1, characterized in that: The application specifically comprises the following steps: mixing sodium butyrate and sucrose solution to obtain a sodium butyrate-sucrose solution; and feeding a bee colony with the obtained sodium butyrate-sucrose solution.

3. The use of sodium butyrate in improving the quality of queen bees according to claim 2, characterized in that: The concentration of sodium butyrate in the sodium butyrate-sucrose solution is 10-40 mM / L.

4. The use of sodium butyrate in improving the quality of queen bees according to claim 3, characterized in that: The concentration of sodium butyrate in the sodium butyrate-sucrose solution is 40 mM / L.

5. The use of sodium butyrate in improving the quality of queen bees according to claim 2, characterized in that: The feeding amount is 300 to 500 mL per group per day.

6. The use of sodium butyrate in improving the quality of queen bees according to claim 2, characterized in that: The best time to start feeding is 7 to 10 days before queen rearing.

7. The use of sodium butyrate in improving the quality of queen bees according to claim 2, characterized in that: The best time to end feeding is 4 to 5 days after worm transfer.

8. The use of sodium butyrate in improving the quality of queen bees according to claim 2, characterized in that: The bee colony is a breeding colony.

9. The use of sodium butyrate in improving the quality of queen bees according to claim 2, characterized in that: The preparation method of the sodium butyrate-sucrose solution is specifically as follows: firstly, sodium butyrate is dissolved in ultrapure water, and then sucrose syrup is added to make up the volume to obtain the sodium butyrate-sucrose solution.

Images