Method for preventing potato fusarium wilt by using ABT rooting powder

Abstract

This invention provides a method for controlling potato verticillium wilt using ABT rooting powder, comprising: fully dissolving ABT rooting powder in alcohol, diluting with distilled water, and setting aside; removing 2-week-old Désirée and Zhongshu 19 test-tube seedlings, cutting off the roots, soaking the base of the potato stems in ABT rooting powder, planting them in a hydroponic box, observing the growth of the potato roots, and selecting a suitable concentration of ABT rooting powder for later use; planting Désirée and Zhongshu 19 test-tube seedlings, and when the plants have grown to 4-6 true leaves, preparing a suspension of non-alfalfa Verticillium Vn011 spores and irrigating the base of the potato stems; irrigating the base of the potato stems with ABT rooting powder 3 days after inoculation with the pathogen; calculating the disease severity and disease index 20, 30, and 40 days after inoculation with the pathogen; harvesting potatoes 50 days after inoculation with the pathogen, measuring yield, and observing and statistically analyzing the growth of the potato roots. This invention utilizes ABT rooting powder to promote the rapid growth of potato roots and plants, thereby achieving the purpose of preventing and controlling potato verticillium wilt.

Description

Technical Field

[0001] This invention relates to the field of biological control technology for potato Verticillium wilt, specifically to a method for controlling potato Verticillium wilt using ABT rooting powder. Background Technology

[0002] Potatoes are my country's fourth largest food crop, playing a strategic role in ensuring national food security and promoting regional economic development. In recent years, my country's potato planting area has been continuously increasing. However, due to limited arable land and continuous cropping, Verticillium wilt, a soil-borne disease caused by Verticillium fungi, has become increasingly serious, impacting potato production to varying degrees in major potato-producing areas of northern my country.

[0003] Verticillium dahliae and Verticillium nonalfalfae are the main pathogens causing Verticillium wilt in potatoes. These pathogens can survive in the soil for over a decade in dormant forms such as microsclerotia or dormant hyphae. When conditions are suitable, they germinate into mycelium and invade the vascular bundles of the host's roots, causing systemic infection.

[0004] Potato Verticillium wilt, also known as "premature death disease," typically progresses from the bottom up after infection. The lower leaves are the first to show symptoms, sometimes only one main stem or one leaf on one side of the stem wilts first. In the early stages, chlorotic yellow spots appear at the leaf tips, along the leaf margins, and between the midribs, gradually deepening in color, while the midrib and surrounding leaf tissue remain green, resembling a "watermelon rind." As the disease worsens, the entire leaf turns from yellow to brown, eventually drying out completely without falling off. In later stages, the vascular bundles of the main stem turn brown, leading to premature wilting and death of the entire potato plant. In the underground tubers, infection begins at the junction of the stolon and tuber; when a diseased potato is cut crosswise, the browned vascular bundles are clearly visible. Verticillium wilt damages potato plants in various ways: mild cases stunt growth and premature death, leading to reduced yield; severe cases cause tubers to turn brown, resulting in significant yield reduction and lower market value.

[0005] Currently, the main methods for controlling potato verticillium wilt are chemical and biological control measures. Chemical control methods, such as methyl bromide and methyl isothiocyanate, are widely used as soil fumigants before crop planting; however, excessive application of chemical pesticides has had a serious impact on the ecological environment. Biological control methods, such as Bacillus, Pseudomonas, and Trichoderma harzianum, are also widely used, but their effectiveness in the field is not obvious or inconsistent in different regions, and the environmental safety risks associated with biological control are still unclear.

[0006] ABT rooting powder is an important auxin-based plant growth regulator, mainly containing two active ingredients: indoleacetic acid (IAA) and naphthaleneacetic acid (NAA). IAA plays a crucial role in plant cell division, elongation, differentiation, seed germination, root development, and vegetative growth. NAA is a broad-spectrum plant growth regulator that rapidly promotes cell division and expansion, inducing adventitious root formation, thus significantly promoting growth and rooting. The inventors of this application have found, through research, that there are currently no reports on the use of ABT rooting powder for preventing potato verticillium wilt. Summary of the Invention

[0007] To address the high costs and environmental impacts associated with existing chemical and biological control methods for potato Verticillium wilt, this invention provides a method for controlling potato Verticillium wilt using ABT rooting powder. This method utilizes ABT rooting powder to promote rapid growth of potato roots and plants, thereby achieving the goal of controlling potato Verticillium wilt.

[0008] To solve the above-mentioned technical problems, the present invention adopts the following technical solution:

[0009] A method for controlling potato Verticillium wilt using ABT rooting powder includes:

[0010] 1) Screening of ABT rooting powder concentration: Dissolve 1g of ABT rooting powder in 100mL of 75% alcohol, and then dilute with distilled water to a concentration of 0mg / L to 80mg / L. Seal with aluminum foil and store in a cool place for later use. Take out Désirée and Zhongshu 19 test-tube seedlings with a seedling age of 2 weeks, cut off the roots, soak the base of the potato stem in ABT rooting powder with a concentration of 0mg / L to 80mg / L for 20 minutes, and then plant the potato test-tube seedlings in a hydroponic box. Observe the growth of potato roots after 7 days and screen out ABT rooting powder with a concentration of 5mg / L to 40mg / L for later use.

[0011] 2) ABT rooting powder for the control of potato verticillium wilt:

[0012] 21) Inoculation of potato plants with non-alfalfa verticillium: Two-week-old Désirée and Zhongshu 19 test-tube seedlings were planted in pots. Two to three weeks after planting, when the potatoes had 4–6 true leaves, a concentration of 1×10⁻⁶ was prepared. 7 A suspension of non-alfalfa Verticillium Vn011 spores at cfu / mL was taken and 80 mL was poured onto the base of potato stems. An equal amount of distilled water was then used to inoculate control plants.

[0013] 22) ABT rooting powder treatment: Three days after inoculation with non-alfalfa Verticillium Vn011 (the pathogen), 100 mL of distilled water (0 mg / L and 5 mg / L to 40 mg / L of ABT rooting powder) was applied to the base of the potato stems. Disease severity was assessed and disease index was calculated 20, 30, and 40 days after inoculation. Fifty days after inoculation, stem segments from the base of the potato stems were taken for pathogen isolation. Potatoes were harvested and yield was measured. The growth of the potato root system was observed and statistically analyzed.

[0014] Furthermore, the ABT rooting powder added in step 1) was purchased from Beijing Aibit Biotechnology Co., Ltd.

[0015] Further, in step 1), 1g of ABT rooting powder is dissolved in 100mL of 75% alcohol, and then diluted with distilled water to concentrations of 0mg / L, 5mg / L, 10mg / L, 20mg / L, 40mg / L, 60mg / L, and 80mg / L, respectively. The solutions are then sealed in aluminum foil and placed in a cool place for later use. Two-week-old Désirée and Zhongshu 19 test-tube seedlings are taken out, their roots are cut off, and the base of the potato stems is soaked in distilled water at concentrations of 0mg / L and 5mg / L, 10mg / L, 20mg / L, 40mg / L, 60mg / L, and 80mg / L for 20 minutes. The potato test-tube seedlings are then planted in hydroponic boxes, and the root growth of the potatoes is observed after 7 days. Finally, ABT rooting powder at concentrations of 5mg / L, 10mg / L, 20mg / L, and 40mg / L is selected for later use.

[0016] In step 22), three days after inoculating with non-alfalfa Verticillium Vn011 (the pathogen), 100 mL of distilled water (0 mg / L and ABT rooting powder at concentrations of 5 mg / L, 10 mg / L, 20 mg / L, and 40 mg / L) is respectively used to irrigate the base of the potato stem.

[0017] Furthermore, in step 22), 3 days after inoculating with non-alfalfa Verticillium Vn011 (the pathogen), 100 mL of ABT rooting powder with a concentration of 10 mg / L is measured and applied to the base of the potato stem.

[0018] Furthermore, in step 22), the root length, root volume, root surface area, and number of root tips of *Désirée* treated with concentrations of 5 mg / L, 10 mg / L, 20 mg / L, and 40 mg / L increased by 93.28%–290.03%, 43.06%–336.87%, 78.05%–315.69%, and 46.44%–152.22%, respectively, compared to the treatment with distilled water (0 mg / L). For *Zhongshu 19*, the root length, root volume, root surface area, and number of root tips increased by 29.21%–173.94%, 49.78%–345.96%, 232.76%–578.99%, and 69.49%–388.54%, respectively.

[0019] Compared with existing technologies, the method for controlling potato Verticillium wilt using ABT rooting powder provided by this invention involves diluting and screening different concentrations of ABT rooting powder and irrigating the base of potato stems inoculated with non-alfalfa Verticillium Vn011. Based on data on the severity of disease in the aboveground parts of the potato plant, as well as the root system and yield, it is shown that ABT rooting powder treatment can promote potato root growth and development, delay the progression of Verticillium wilt, prolong plant death, and reduce yield loss caused by premature plant death. Among them, ABT rooting powder concentrations of 5 mg / L to 40 mg / L significantly promoted potato root and plant growth; the treatment concentration of ABT rooting powder at 10 mg / L showed the best disease resistance effect, with indicators such as disease severity, disease index, and root growth indicating that this concentration significantly enhanced potato Verticillium wilt resistance and delayed plant death; the treatment concentration of ABT rooting powder at 10 mg / L resulted in the lowest yield loss, with yield reduction rates of 31.30% and 34.36% for the moderately resistant variety Zhongshu 19 and the susceptible variety Désirée, respectively, significantly reducing yield loss and controlling potato Verticillium wilt. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the phenotype of potatoes after treatment with different concentrations of ABT rooting powder provided by the present invention.

[0021] Figure 2 This is a schematic diagram illustrating the effect of different concentrations of ABT rooting powder provided by this invention in controlling potato Verticillium wilt.

[0022] Figure 3 This is a schematic diagram showing the proportion of potato Verticillium wilt disease severity and disease index after treatment with different concentrations of ABT rooting powder provided by this invention.

[0023] Figure 4 This is a schematic diagram illustrating the phenotype and data analysis of potato roots after treatment with different concentrations of ABT rooting powder provided by the present invention.

[0024] Figure 5This is a schematic diagram showing the potato yield after treatment with different concentrations of ABT rooting powder provided by the present invention. Detailed Implementation

[0025] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below with reference to specific illustrations.

[0026] This invention provides a method for controlling potato verticillium wilt using ABT rooting powder, comprising:

[0027] 1) Screening of ABT rooting powder concentration: Dissolve 1g of ABT rooting powder in 100mL of 75% alcohol, and then dilute with distilled water to a concentration of 0mg / L to 80mg / L. Seal with aluminum foil and store in a cool place for later use. Take out Désirée and Zhongshu 19 test-tube seedlings with a seedling age of 2 weeks, cut off the roots, soak the base of the potato stem in ABT rooting powder with a concentration of 0mg / L to 80mg / L for 20 minutes, and then plant the potato test-tube seedlings in a hydroponic box. Observe the growth of potato roots after 7 days and screen out ABT rooting powder with a concentration of 5mg / L to 40mg / L for later use.

[0028] 2) ABT rooting powder for the control of potato verticillium wilt:

[0029] 21) Inoculation of potato plants with non-alfalfa verticillium: Two-week-old Désirée and Zhongshu 19 test-tube seedlings were planted in pots. Two to three weeks after planting, when the potatoes had 4–6 true leaves, a concentration of 1×10⁻⁶ was prepared. 7 A suspension of non-alfalfa Verticillium Vn011 spores at cfu / mL was taken and 80 mL was poured onto the base of potato stems. An equal amount of distilled water was then used to inoculate control plants.

[0030] 22) ABT rooting powder treatment: Three days after inoculation with non-alfalfa Verticillium Vn011 (the pathogen), 100 mL of distilled water (0 mg / L and 5 mg / L to 40 mg / L of ABT rooting powder) was applied to the base of the potato stems. Disease severity was assessed and disease index was calculated 20, 30, and 40 days after inoculation. Fifty days after inoculation, stem segments from the base of the potato stems were taken for pathogen isolation. Potatoes were harvested and yield was measured. The growth of the potato root system was observed and statistically analyzed.

[0031] As a specific embodiment, the ABT rooting powder added in step 1) is purchased from Beijing Aibit Biotechnology Co., Ltd., and specifically, ABT rooting powder No. 1 produced by the company can be used.

[0032] As a specific embodiment, in step 1), 1g of ABT rooting powder is dissolved in 100mL of 75% alcohol, and then diluted with distilled water to concentrations of 0mg / L, 5mg / L, 10mg / L, 20mg / L, 40mg / L, 60mg / L, and 80mg / L, respectively. The solutions are then sealed in aluminum foil and placed in a cool place for later use. Two-week-old Désirée and Zhongshu 19 test-tube seedlings are taken out, their roots are cut off, and the base of the potato stems is soaked in distilled water at concentrations of 0mg / L and 5mg / L, 10mg / L, 20mg / L, 40mg / L, 60mg / L, and 80mg / L for 20 minutes. The potato test-tube seedlings are then planted in a hydroponic box, and the root growth of the potatoes is observed after 7 days. By comparing the growth and development phenotypes of hydroponic seedlings of the susceptible potato variety Désirée and the moderately resistant variety Zhongshu 19 after treatment with different concentrations of ABT rooting powder, the results showed that at concentrations of 5 mg / L to 40 mg / L, the higher the concentration of ABT rooting powder, the more vigorous the potato plant growth and the more developed the root system, with the best results observed at 10 mg / L ABT rooting powder. With further increases in ABT rooting powder concentration, plant growth slowed down; and when the concentration reached 60 mg / L, plant growth was significantly inhibited, specifically as follows: Figure 1 As shown in Figure A-1B. In the figure, A represents the Désirée plant phenotype, B represents the Zhongshu 19 plant phenotype, 0-80 mg / L represents the 0-80 mg / L ABT rooting powder treatment, the scale bar = 10 cm, and finally, ABT rooting powder concentrations of 5 mg / L, 10 mg / L, 20 mg / L, and 40 mg / L were selected for use.

[0033] As a specific embodiment, in step 22), three days after inoculation with non-alfalfa Verticillium Vn011 (the pathogen), 100 mL of distilled water (0 mg / L) and ABT rooting powder at concentrations of 5 mg / L, 10 mg / L, 20 mg / L, and 40 mg / L were respectively applied to the base of the potato stems. The disease phenotype of each treatment was investigated 30 days later. The results showed that compared to the 0 mg / L distilled water treatment, the application of ABT rooting powder at concentrations of 5 mg / L, 10 mg / L, 20 mg / L, and 40 mg / L significantly delayed the development of Verticillium wilt and significantly delayed plant mortality in the susceptible variety "Désirée" and the moderately resistant variety "Zhongshu 19". The 10 mg / L treatment showed the best effect, as detailed below. Figure 2 As shown in the figure, A represents the phenotype of "Désirée" 30 days after inoculation with non-alfalfa Verticillium Vn011; B represents the phenotype of "Zhongshu 19" 30 days after inoculation with non-alfalfa Verticillium Vn011; CK represents healthy potatoes; Vn011+0~40mg / L represents potatoes inoculated with Vn011 and treated with ABT rooting powder at a concentration of 0~40mg / L; scale bar = 12cm.

[0034] As a specific embodiment, in step 22), 3 days after inoculating with non-alfalfa Verticillium Vn011 (the pathogen), 100 mL of ABT rooting powder with a concentration of 10 mg / L is measured and applied to the base of the potato stem. The potato phenotypic effect is best after treatment with 10 mg / L.

[0035] As a specific embodiment, in step 22), the disease severity of potatoes was statistically analyzed 20, 30, and 40 days after inoculation with non-alfalfa verticillium. The results showed that the disease severity of potatoes treated with ABT rooting powder was significantly lower than that of the control. Under the 10 mg / L treatment, the proportion of highly diseased plants in Désirée and Zhongshu 19 was the lowest at each time point. Specifically, as shown below... Figure 3 A and Figure 3 As shown in Figure C. The disease index trends showed that with prolonged inoculation time, the disease index of all treatments in both Désirée and Zhongshu 19 gradually increased, indicating a progressively more severe disease. Compared to the distilled water treatment (0 mg / L), the disease indices of treatments with concentrations from 5 mg / L to 40 mg / L were significantly lower at all time points. The 10 mg / L treatment had the lowest disease index. (Specific details are shown in Figure C.) Figure 3 B and Figure 3 As shown in D. In summary, ABT rooting powder treatment can delay the development of potato Verticillium wilt and reduce the disease index, with a concentration of 10 mg / L ABT rooting powder showing the best effect. In the figure, A and C represent the disease levels of Désirée and Zhongshu 19, respectively; B and D represent the disease indices of Désirée and Zhongshu 19, respectively; 20 dpi, 30 dpi, and 40 dpi represent 20, 30, and 40 days after inoculation with Vn011, respectively; CK represents healthy potatoes; Vn011 + 0–40 mg / L represents potatoes inoculated with Vn011 and treated with ABT rooting powder at concentrations of 0–40 mg / L.

[0036] As a specific embodiment, in step 22), the root systems of various potato varieties after inoculation with non-alfalfa Verticillium Vn011 were further analyzed. Compared with treatment with distilled water (0 mg / L ABT rooting powder), the potato roots treated with concentrations of 5 mg / L to 40 mg / L showed significantly more and better growth. Specifically, as shown below... Figure 4As shown in the figure, A and B represent the root phenotypes of Désirée and Zhongshu 19, respectively, with a scale bar of 10 cm; C represents the total root length; D represents the total root volume; E represents the total root surface area; F represents the number of root tips; CK represents healthy potatoes; Vn011+0~40mg / L represents potatoes inoculated with Vn011 and treated with ABT rooting powder at a concentration of 0~40mg / L. Specifically, compared with the treatment with distilled water (0 mg / L), the root length, root volume, root surface area, and number of root tips of *Désirée* treated with concentrations of 5 mg / L, 10 mg / L, 20 mg / L, and 40 mg / L increased by 93.28%–290.03%, 43.06%–336.87%, 78.05%–315.69%, and 46.44%–152.22%, respectively; while the root length, root volume, root surface area, and number of root tips of *Zhongshu 19* increased by 29.21%–173.94%, 49.78%–345.96%, 232.76%–578.99%, and 69.49%–388.54%, respectively. Among the tested parameters, the 10 mg / L treatment showed the greatest increase in root length, root volume, root surface area, and root tip number for both materials. As the concentration of ABT rooting powder increased further, the increase in each parameter decreased. Therefore, ABT rooting powder treatment can promote potato root growth and development, delay the disease progression in the above-ground potato plants, and enhance the potato's disease resistance. The 10 mg / L concentration of ABT rooting powder was the most effective.

[0037] As a specific embodiment, in step 22), to further clarify the effect of ABT rooting powder on the yield of infected potatoes, the weight of individual tubers and the number of tubers per plant were statistically analyzed for each treatment. The results showed that compared with healthy potatoes (CK), the weight of individual tubers in both Désirée and Zhongshu 19 was significantly reduced after inoculation with the pathogen, while the number of tubers per plant did not decrease significantly. Specifically, as follows... Figure 5 As shown in Table 1, A represents the yield of 16 Désirée plants; B represents the yield of 16 Zhongshu 19 plants; scale bar = 5 cm; CK represents healthy potatoes; Vn011 + 0–40 mg / L represents potatoes inoculated with Vn011 and treated with ABT rooting powder at concentrations of 0–40 mg / L. Among these, the weight of individual potatoes per Désirée plant decreased by 74.82%, 62.26%, 34.36%, 55.65%, and 69.60% respectively compared to CK at concentrations of 0 mg / L, 5 mg / L, 10 mg / L, 20 mg / L, and 40 mg / L; and by 65.34%, 61.69%, 31.30%, 48.98%, and 56.51% respectively for Zhongshu 19 plants. These results indicate that ABT rooting powder treatment can reduce yield loss in potatoes caused by Verticillium wilt. Among them, the potato yield loss was minimal when the ABT rooting powder concentration was 10 mg / L.

[0038] Table 1. Analysis of potato yield after ABT rooting powder treatment

[0039]

[0040] Based on data on the severity of disease in the above-ground parts of the potato plant, as well as the root system and yield, it was found that ABT rooting powder treatment can promote potato root growth and development, delay the progression of Verticillium wilt, postpone plant death, and reduce yield loss caused by premature plant mortality. The optimal concentration of ABT rooting powder at 10 mg / L resulted in the best control effect against Verticillium wilt.

[0041] Compared with existing technologies, the method for controlling potato Verticillium wilt using ABT rooting powder provided by this invention involves diluting and screening different concentrations of ABT rooting powder and irrigating the base of potato stems inoculated with non-alfalfa Verticillium Vn011. Based on data on the severity of disease in the aboveground parts of the potato plant, as well as the root system and yield, it is shown that ABT rooting powder treatment can promote potato root growth and development, delay the progression of Verticillium wilt, prolong plant death, and reduce yield loss caused by premature plant death. Among them, ABT rooting powder concentrations of 5 mg / L to 40 mg / L significantly promoted potato root and plant growth; the treatment concentration of ABT rooting powder at 10 mg / L showed the best disease resistance effect, with indicators such as disease severity, disease index, and root growth indicating that this concentration significantly enhanced potato Verticillium wilt resistance and delayed plant death; the treatment concentration of ABT rooting powder at 10 mg / L resulted in the lowest yield loss, with yield reduction rates of 31.30% and 34.36% for the moderately resistant variety Zhongshu 19 and the susceptible variety Désirée, respectively, significantly reducing yield loss and controlling potato Verticillium wilt.

[0042] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A method for controlling potato verticillium wilt while simultaneously promoting potato root and plant growth using ABT rooting powder, characterized in that, The method includes the following steps: 1) Screening of ABT rooting powder concentration: Dissolve 1g of ABT rooting powder in 100mL of 75% alcohol, and then dilute with distilled water to a concentration of 0mg / L to 80mg / L. Seal with aluminum foil and store in a cool place for later use. Take out Désirée and Zhongshu 19 test-tube seedlings with a seedling age of 2 weeks, cut off the roots, soak the base of the potato stem in ABT rooting powder with a concentration of 0mg / L to 80mg / L for 20 minutes, and then plant the potato test-tube seedlings in a hydroponic box. Observe the growth of potato roots after 7 days and screen out ABT rooting powder with a concentration of 5mg / L to 40mg / L for later use. 2) ABT rooting powder for the control of potato verticillium wilt: 21) Inoculation of potato plants with non-alfalfa verticillium: Two-week-old Désirée and Zhongshu 19 test-tube seedlings were planted in pots. Two to three weeks after planting, when the potatoes had 4–6 true leaves, a concentration of 1×10⁻⁶ was prepared. 7 A suspension of non-alfalfa Verticillium Vn011 spores at cfu / mL was taken and 80 mL was poured onto the base of potato stems. An equal amount of distilled water was then used to inoculate control plants. 22) ABT rooting powder treatment: Three days after inoculation with non-alfalfa Verticillium Vn011 (the pathogen), 100 mL of distilled water (0 mg / L and 5 mg / L to 40 mg / L of ABT rooting powder) was applied to the base of the potato stems. Disease severity was assessed and disease index was calculated 20, 30, and 40 days after inoculation. Fifty days after inoculation, stem segments from the base of the potato stems were taken for pathogen isolation. Potatoes were harvested and yield was measured. The growth of the potato root system was observed and analyzed. The pathogen causing potato verticillium wilt is not alfalfa verticillium.

2. The method according to claim 1, characterized in that, The ABT rooting powder added in step 1) was purchased from Beijing Aibit Biotechnology Co., Ltd.

3. The method according to claim 1, characterized in that, In step 1), 1g of ABT rooting powder is dissolved in 100mL of 75% alcohol. It is then diluted with distilled water to concentrations of 0mg / L, 5mg / L, 10mg / L, 20mg / L, 40mg / L, 60mg / L, and 80mg / L, respectively. The solutions are sealed in aluminum foil and stored in a cool, dark place. Two-week-old Désirée and Zhongshu 19 test-tube seedlings are removed, their roots are cut off, and the base of the potato stems is soaked in distilled water at concentrations of 0mg / L and 5mg / L, 10mg / L, 20mg / L, 40mg / L, 60mg / L, and 80mg / L for 20 minutes. The potato test-tube seedlings are then planted in a hydroponic container. After 7 days, the root growth of the potatoes is observed, and finally, ABT rooting powder concentrations of 5mg / L, 10mg / L, 20mg / L, and 40mg / L are selected for use. In step 22), after inoculating with non-alfalfa verticillium Vn01 for 13 days, 100 mL of distilled water (0 mg / L and ABT rooting powder at concentrations of 5 mg / L, 10 mg / L, 20 mg / L, and 40 mg / L) is respectively used to irrigate the base of the potato stem.

4. The method according to claim 3, characterized in that, In step 22), after inoculating with non-alfalfa verticillium Vn01 for 13 days, 100 mL of ABT rooting powder with a concentration of 10 mg / L is measured and applied to the base of the potato stem.

5. The method according to claim 3, characterized in that, In step 22), the root length, root volume, root surface area, and number of root tips of *Désirée* treated with concentrations of 5 mg / L, 10 mg / L, 20 mg / L, and 40 mg / L increased by 93.28%–290.03%, 43.06%–336.87%, 78.05%–315.69%, and 46.44%–152.22%, respectively, compared to the treatment with distilled water (0 mg / L). For *Zhongshu 19*, the root length, root volume, root surface area, and number of root tips increased by 29.21%–173.94%, 49.78%–345.96%, 232.76%–578.99%, and 69.49%–388.54%, respectively.

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