Seedling raising method for polygonatum sibiricum seeds

Abstract

The invention provides a rhizoma polygonati seed seedling raising method. The rhizoma polygonati seed seedling raising method comprises the following steps: S1, obtaining rhizoma polygonati seeds; s2, preparing a seed germination promoting solution, and soaking the polygonatum sibiricum seeds in the seed germination promoting solution; s3, mixing the disinfected polygonatum sibiricum seeds with river sand to obtain a seed mixture; s4, mixing a substrate, rooting powder and plant ash to prepare culture soil, and pouring the culture soil into a growth box; s5, spreading the seed mixture prepared in the step S3 on culture soil of a growth box; s6, before emergence of the polygonatum sibiricum seeds, placing the growth box in a shady place, and regularly watering until emergence of the polygonatum sibiricum seeds. According to the method, the prepared seed mixture is directly laid on the culture soil, so that the sand storage and seedling raising processes of the polygonatum sibiricum seeds are synchronously performed in the same growth box, and the process of re-sowing the polygonatum sibiricum seeds after sand storage and germination is reduced, so that the seedling raising time of the polygonatum sibiricum seeds is greatly shortened, and the seedling raising efficiency of the polygonatum sibiricum seeds is improved. The polygonatum sibiricum seeds in the seedling raising mode emerge earlier, and the emergence rate is higher.

Description

Technical Field

[0001] The invention belongs to the technical field of polygonatum seedling cultivation, and particularly relates to a polygonatum seed seedling cultivation method. Background Art

[0002] Polygonatum sibiricum is a perennial herbaceous plant of the genus Polygonatum in the Liliaceae family. It has both medicinal and edible value. It is one of the basal plants of the Chinese medicines Polygonatum sibiricum, Polygonatum yunnanensis, and Polygonatum multiflorum. Its dried roots are used as medicine. Its rhizomes contain effective ingredients such as polysaccharides, flavonoids, steroidal saponins, anthraquinones, and alkaloids. It has anti-inflammatory, anti-tumor, osteoporosis prevention, qi-tonifying and yin-nourishing, spleen-strengthening, lung-moistening, and kidney-benefiting effects. Under the background of great health, the Polygonatum sibiricum industry has entered a new era of rapid development. The main propagation methods of Polygonatum sibiricum are seed propagation and rhizome propagation. Among them, rhizome propagation will consume a large amount of medicinal resources and lead to problems such as degradation of germplasm resources, poor stress resistance, and aggravation of diseases and insect pests. Seed propagation has great advantages in preserving Polygonatum sibiricum germplasm resources and solving problems such as shortage of wild resources. However, the dormancy characteristics of Polygonatum sibiricum seeds greatly restrict the breeding process of Polygonatum sibiricum seedlings, becoming a bottleneck restricting the rapid development of the industry.

[0003] Under conventional sowing conditions, Polygonatum cyrtonema seeds generally need to be stored in sand for more than 6 months before they can break dormancy and germinate normally. Their dormancy is related to the physiological after-ripening of the embryo. Generally, stratification treatment can promote the morphological development of the embryo. In this process, the inhibitory substances inside the seeds are decomposed, the levels of endogenous hormones such as gibberellins (GA3) and abscisic acid (ABA) change, and the GA3 / ABA ratio differs, causing the Polygonatum cyrtonema seeds to change from a dormant state to a dormant release state; however, the traditional sand storage method takes too long for the seed germination, which affects the Polygonatum cyrtonema seed seedling process. Summary of the invention

[0004] The purpose of the present invention is to provide a method for raising seedlings of Polygonatum cyrtonema seeds, which can at least solve some of the defects in the prior art.

[0005] To achieve the above object, the present invention adopts the following technical solution:

[0006] A method for raising seedlings of polygonatum sibiricum seeds comprises the following steps:

[0007] S1. Obtaining Polygonatum sibiricum seeds;

[0008] S2, preparing a seed germination promoting solution, and soaking the obtained Polygonatum cyrtonema seeds in the seed germination promoting solution;

[0009] S3, disinfecting the soaked polygonatum seeds, and then mixing the disinfected polygonatum seeds with river sand to obtain a seed mixture;

[0010] S4, mix the substrate, rooting powder and wood ash to make culture soil, and pour it into the growth box;

[0011] S5, spreading the seed mixture obtained in step S3 on the culture soil in the growth chamber;

[0012] S6. Before the Polygonatum sibiricum seeds germinate, place the growth box in a shaded place and water it regularly until the Polygonatum sibiricum seeds germinate.

[0013] Furthermore, in step S1, a robust Polygonatum sibiricum plant that has grown for more than 5 years is selected as a seed collection mother plant, and the berries are collected in autumn when they turn black and ripe. The berries are naturally piled up and fermented to allow the berry pulp to rot, and the internal seeds are rubbed out to obtain Polygonatum sibiricum seeds.

[0014] Furthermore, in step S2, the seed germination promoting solution includes carbendazim, rooting powder, gibberellin and fluridone, wherein the gibberellin concentration is 450-550 mg / L, the fluridone concentration is 200-300 mg / L, the carbendazim concentration is 800-850 times diluted solution, and the rooting powder concentration is 5-10 mg / L.

[0015] Furthermore, in step S2, the temperature of the soaking treatment of the Polygonatum sibiricum seeds in the seed germination promoting liquid is room temperature and the soaking time is 24 hours.

[0016] Furthermore, in step S3, the disinfection process is to use 2-3% sodium hypochlorite to disinfect the polygonatum seeds for 5-10 minutes, and then take them out and rinse them with distilled water.

[0017] Furthermore, in step S3, the mass ratio of polygonatum seeds to river sand is 1:3.

[0018] Furthermore, in step S4, the amount of plant ash in the culture soil is 500-600 g / m 2 The dosage of rooting powder is 5-10 mg per kilogram of substrate.

[0019] Furthermore, in step S5, the thickness of the seed mixture spread on the culture soil is 1-2 cm.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] (1) The method for raising seedlings of Polygonatum sibiricum provided by the present invention directly spreads the prepared seed mixture on the culture soil, so that the sand storage and seedling raising processes of Polygonatum sibiricum seeds are carried out synchronously in the same growth chamber, thereby reducing the process of re-sowing the Polygonatum sibiricum seeds after germination in the sand storage, thereby greatly shortening the seedling raising time of Polygonatum sibiricum seeds. In addition, the Polygonatum sibiricum seeds raised by this seedling raising method of the present invention germinate earlier and have a higher germination rate.

[0022] (2) The method for raising seedlings of Polygonatum cyrtonema provided by the present invention can significantly shorten the seed storage time in sand, increase the growth potential of the embryo, and improve the seed germination rate by preparing a seed germination promoting solution containing carbendazim, rooting powder, gibberellin and fluridone and soaking the Polygonatum cyrtonema seeds. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] The present invention provides a method for raising seedlings of polygonatum sibiricum seeds, comprising the following steps:

[0025] S1. Obtain Polygonatum sibiricum seeds.

[0026] Specifically, a robust Polygonatum sibiricum plant that has grown for more than 5 years is selected as a seed collection mother plant, and the berries are collected in autumn when they turn black and ripe. The berries are naturally piled up and fermented to make the berry pulp rot, and the internal seeds are rubbed out to obtain the Polygonatum sibiricum seeds.

[0027] S2. prepare a seed germination promoting liquid, and soak the obtained Polygonatum cyrtonema seeds in the seed germination promoting liquid.

[0028] Specifically, the seed germination promoting solution includes carbendazim, rooting powder, gibberellin and fluridone, wherein the gibberellin concentration is 450-550 mg / L, the fluridone concentration is 200-300 mg / L, the carbendazim concentration is 800-850 times diluted solution, and the rooting powder concentration is 5-10 mg / L. The polygonatum sibiricum seeds are placed in the seed germination promoting solution and soaked at room temperature for 24 hours.

[0029] S3, disinfecting the soaked polygonatum seeds, and then mixing the disinfected polygonatum seeds with river sand to obtain a seed mixture.

[0030] Optionally, the polygonatum seeds are disinfected with 2-3% sodium hypochlorite for 5-10 minutes, and then taken out and rinsed with distilled water. The mass ratio of the polygonatum seeds to the river sand is 1:3.

[0031] S4, mix the substrate, rooting powder and wood ash to make culture soil, and pour it into the growth box; the amount of wood ash in the culture soil is 500-600g / m 2 The dosage of rooting powder is 5-10 mg per kilogram of substrate.

[0032] S5. Spread the seed mixture obtained in step S3 on the culture soil of the growth box.

[0033] Preferably, the seed mixture is spread on the culture soil to a thickness of 1-2 cm.

[0034] S6. Before the Polygonatum sibiricum seeds germinate, place the growth box in a shaded place and water it regularly until the Polygonatum sibiricum seeds germinate.

[0035] The effect of the method for raising seedlings of Polygonatum cyrtonema seeds of the present invention will be described below by means of specific examples.

[0036] Embodiment 1:

[0037] This embodiment investigates the effects of different seedling raising methods on the emergence rate of polygonatum seeds, and sets two different treatment processes (i.e., treatment 1 and treatment 2), wherein treatment 1 adopts the method for raising polygonatum seeds of the present invention, and the process is as follows: S1, select plump polygonatum seeds and soak them in a seed germination promoting solution at room temperature for 24 hours; S2, take out the seeds, disinfect the seeds with 3% sodium hypochlorite for 5 minutes, take out and rinse them three times with distilled water; S3, uniformly mix the polygonatum seeds and river sand in a mass ratio of 1:3; S4, prepare a culture soil with a certain proportion of substrate, rooting powder and wood ash, and pour it into the lower layer of the growth box, wherein the rooting powder is 10 mg / kg, the wood ash is 500 g / m 2 ; S5. Spread the mixture of Polygonatum sibiricum seeds, wood ash and river sand on the culture soil of the growth box with a layer thickness of 2 cm. Place it in a shaded place before germination and water it regularly to keep it moist until the Polygonatum sibiricum seeds germinate.

[0038] The process of treatment 2 is roughly the same as that of treatment 1, except that in step S3, the Polygonatum sibiricum seeds and river sand are mixed and placed in a growth box for a certain period of time, and watered regularly to keep them moist; S5, the seeds after germination in the sand storage in step S3 are taken out, sown on the surface of the culture soil in the growth box in step S4, placed in a shaded place before germination, and watered regularly to keep them moist until the Polygonatum sibiricum seeds germinate.

[0039] The germination rate and germination time of the two treatments were compared, and the results are shown in Table 1.

[0040] Table 1: Effects of different seedling raising methods on the germination rate of Polygonatum cyrtonema seeds (unit: %)

[0041]

[0042]

[0043] As can be seen from Table 1, the seedlings in treatment 1 emerged earlier and had a higher emergence rate, which was better than that in treatment 2, indicating that the seedling raising method of the present invention in which sand storage and seedling raising are performed simultaneously is better.

[0044] Embodiment 2:

[0045] This example investigates the effect of the thickness of the seed mixture spread on the culture soil on the emergence rate of Polygonatum cyrtonema seeds. The specific process is as follows:

[0046] Select plump Polygonatum sibiricum seeds and soak them in seed germination promotion liquid at room temperature for 24 hours; take out the seeds, disinfect them with 3% sodium hypochlorite for 5 minutes, take them out and rinse them three times with distilled water; mix the Polygonatum sibiricum seeds and river sand in a mass ratio of 1:3; prepare the culture soil with a certain proportion of substrate, rooting powder and wood ash, and pour it into the lower layer of the growth box, including 10 mg / kg rooting powder and 500 g / m 2 ; The mixture of Polygonatum sibiricum seeds, plant ash and river sand was spread on the culture soil of the growth box, and the layer thickness of the mixture of Polygonatum sibiricum seeds, plant ash and river sand was screened, and the layer thickness of the mixture was set to 1cm, 2cm, 3cm, and 4cm respectively. It was placed in a shaded place before germination, and watered regularly to keep it moist until the Polygonatum sibiricum seeds emerged. The germination rates of the four treatments were compared, and each treatment was repeated 3 times. The results are shown in Table 2.

[0047] Table 2:

[0048]

[0049] As shown in Table 2, when the layer thickness of the mixture is 1-2 cm, the germination rate is as high as 85%, indicating that the most suitable layer thickness of the sand storage mixture is 1-2 cm.

[0050] Embodiment 3:

[0051] In this example, the effects of different concentrations of gibberellins in the seed germination promoting solution on the germination rate of Polygonatum cyrtonema seeds were investigated. The specific process is as follows:

[0052] Select robust Polygonatum sibiricum plants that have grown for more than 5 years as seed collection mother plants, collect the berries when they turn black and mature in autumn, pile up the berries naturally to ferment them to make the berry pulp rot, and wash out the seeds inside; prepare 7 kinds of seed treatment solutions, namely treatment 1: 800 times dilution of carbendazim, 5 mg / L rooting powder, 500 mg / L fluridone; treatment 2: 800 times dilution of carbendazim, 5 mg / L rooting powder, 250 mg / L gibberellin, 500 mg / L fluridone; treatment 3: 800 times dilution of carbendazim, 5 mg / L rooting powder, 450 mg / L gibberellin, fluridone 500mg / L; Treatment 4: 800 times dilution of carbendazim, 5mg / L rooting powder, 500mg / L gibberellin, 500mg / L fluridone; Treatment 5: 800 times dilution of carbendazim, 5mg / L rooting powder, 550mg / L gibberellin, 500mg / L fluridone; Treatment 6: 800 times dilution of carbendazim, 5mg / L rooting powder, 750mg / L gibberellin, 500mg / L fluridone; Treatment 7: 800 times dilution of carbendazim, 5mg / L rooting powder, 1000mg / L gibberellin, 500mg / L fluridone.

[0053] Select plump Polygonatum sibiricum seeds and soak them in the above five seed treatment solutions at room temperature for 24 hours; take out the seeds, disinfect them with 3% sodium hypochlorite for 5 minutes, take them out and rinse them three times with distilled water; mix the Polygonatum sibiricum seeds and river sand at a mass ratio of 1:3; put them into a growth box with sand, water them regularly to keep them moist, and compare the seed germination rates of the five treatments, with 3 replicates for each treatment. The results are shown in Table 3.

[0054] Table 3: Effects of different concentrations of gibberellins on the germination rate of Polygonatum cyrtonema seeds (unit: %)

[0055]

[0056] It can be seen from Table 3 that when the gibberellin concentration is 500 mg / L, the germination rate of Polygonatum sibiricum seeds is the highest, indicating that treatment 4 is the optimal treatment, that is, the seed treatment solution is: 800 times diluted carbendazim, 5 mg / L rooting powder, 500 mg / L gibberellin, and 500 mg / L fluridone.

[0057] Embodiment 4:

[0058] In this example, the effects of different concentrations of fluridone in the seed germination promoting solution on the germination rate of Polygonatum cyrtonema seeds were investigated. The specific process is as follows:

[0059] On the basis of screening the concentration of 500 mg / L gibberellin, the optimal concentration of fluridone for promoting the germination of Polygonatum cyrtonema seeds was further screened, and 7 seed treatment solutions were prepared, with other conditions unchanged. Treatment 1: 800 times dilution of carbendazim, 5 mg / L rooting powder, 500 mg / L gibberellin; Treatment 2: 800 times dilution of carbendazim, 5 mg / L rooting powder, 500 mg / L gibberellin, 50 mg / L fluridone; Treatment 3: 800 times dilution of carbendazim, 5 mg / L rooting powder, 500 mg / L gibberellin, 100 mg / L fluridone L; Treatment 4: 800 times dilution of carbendazim, 5 mg / L rooting powder, 500 mg / L gibberellin, 200 mg / L fluridone; Treatment 5: 800 times dilution of carbendazim, 5 mg / L rooting powder, 500 mg / L gibberellin, 250 mg / L fluridone; Treatment 6: 800 times dilution of carbendazim, 5 mg / L rooting powder, 500 mg / L gibberellin, 300 mg / L fluridone; Treatment 7: 800 times dilution of carbendazim, 5 mg / L rooting powder, 500 mg / L gibberellin, 500 mg / L fluridone.

[0060] Select plump Polygonatum sibiricum seeds and soak them in the above 5 seed treatment solutions at room temperature for 24 hours; take out the seeds, disinfect them with 3% sodium hypochlorite for 5 minutes, take them out and rinse them three times with distilled water; mix the Polygonatum sibiricum seeds and river sand at a mass ratio of 1:3; put them into the growth box with sand, water them regularly to keep them moist, and compare the germination rates of the 5 treatments, with 3 replicates for each treatment. The results are shown in Table 4.

[0061] Table 4: Effects of different concentrations of fluridone on the germination rate of Polygonatum cyrtonema seeds (unit: %)

[0062]

[0063] It can be seen from Table 4 that when the concentration of fluridone is 250 mg / L, the germination rate of Polygonatum sibiricum seeds is the highest, indicating that treatment 5 is the optimal treatment, that is, the seed treatment solution is: 800 times diluted carbendazim, 5 mg / L rooting powder, 500 mg / L gibberellin, and 250 mg / L fluridone.

[0064] The above examples are merely illustrative of the present invention and do not constitute a limitation on the protection scope of the present invention. All designs that are the same or similar to the present invention fall within the protection scope of the present invention.

Claims

1. A method for raising seedlings of Polygonatum cyrtonema, characterized in that: The steps include: S1. Obtaining Polygonatum sibiricum seeds; S2, preparing a seed germination promoting solution, and soaking the obtained Polygonatum cyrtonema seeds in the seed germination promoting solution; S3, disinfecting the soaked polygonatum seeds, and then mixing the disinfected polygonatum seeds with river sand to obtain a seed mixture; S4, mix the substrate, rooting powder and wood ash to make culture soil, and pour it into the growth box; S5, spreading the seed mixture obtained in step S3 on the culture soil in the growth chamber; S6. Before the Polygonatum sibiricum seeds germinate, place the growth box in a shaded place and water it regularly until the Polygonatum sibiricum seeds germinate.

2. The method for raising seedlings of polygonatum sibiricum seeds as claimed in claim 1, characterized in that: In step S1, a robust Polygonatum sibiricum plant that has grown for more than 5 years is selected as a seed collection mother plant, and the berries are collected in autumn when they turn black and ripe. The berries are naturally piled up and fermented to make the berry pulp rot, and the internal seeds are rubbed out to obtain Polygonatum sibiricum seeds.

3. The method for raising seedlings of polygonatum sibiricum seeds as claimed in claim 1, characterized in that: In step S2, the seed germination promoting solution includes carbendazim, rooting powder, gibberellin and fluridone, wherein the gibberellin concentration is 450-550 mg / L, the fluridone concentration is 200-300 mg / L, the carbendazim concentration is 800-850 times diluted solution, and the rooting powder concentration is 5-10 mg / L.

4. The method for raising seedlings of polygonatum sibiricum seeds as claimed in claim 3, characterized in that: In step S2, the temperature of soaking the Polygonatum sibiricum seeds in the seed germination promoting liquid is room temperature and the soaking time is 24 hours.

5. The method for raising seedlings of polygonatum sibiricum seeds as claimed in claim 1, characterized in that: The disinfection process in step S3 is to disinfect the polygonatum seeds with sodium hypochlorite at a concentration of 2-3% for 5-10 minutes, and then take them out and rinse them with distilled water.

6. The method for raising seedlings of polygonatum sibiricum seeds as claimed in claim 1, characterized in that: In step S3, the mass ratio of polygonatum seeds to river sand is 1:

3.

7. The method for raising seedlings of polygonatum sibiricum seeds as claimed in claim 1, characterized in that: In step S4, the amount of plant ash in the culture soil is 500-600g / m 2 The dosage of rooting powder is 5-10 mg per kilogram of substrate.

8. The method for raising seedlings of polygonatum sibiricum seeds as claimed in claim 1, characterized in that: In step S5, the thickness of the seed mixture spread on the culture soil is 1-2 cm.

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