Nutritional supplement for preventing hypertension, hyperlipidemia, hyperglycemia, hyperglycemia and hyperglycemia, tumors and obesity and improving immunity and tablet and preparation method thereof

Abstract

The invention provides a nutritional supplement for preventing hypertension, hyperlipidemia, hyperglycemia, hyperglycemia, tumors and obesity and improving immunity and a tablet and a preparation method thereof, and belongs to the field of healthy food processing. On one hand, the invention provides the nutritional supplement for preventing three highs, tumors and obesity and improving immunity, and the nutritional supplement comprises the following raw materials: chlorella pyrenoidosa, xylooligosaccharide, yeast beta-glucan and tea leaf theanine; on the second aspect, the invention also provides a preparation method of a tablet of the nutritional supplement for preventing hypertension, hyperglycemia, hyperlipidemia, tumors and obesity and improving immunity; the invention further provides application of the nutritional supplement or tablets of the nutritional supplement in preparation of food or health food for preventing hypertension, hyperglycemia and hyperlipidemia and / or tumors and / or obesity and / or improving immunity. Compared with the prior art, the nutritional supplement for preventing hypertension, hyperglycemia, hyperlipidemia, tumor and obesity and improving immunity has remarkable improvement in the aspects of preventing hypertension, hyperlipidemia, tumor and obesity and improving immunity indexes.

Description

Technical Field

[0001] The present invention relates to the technical field of nutritional supplements, and in particular to a nutritional supplement for preventing three highs, tumors, obesity and improving immunity, and a tablet thereof and a preparation method thereof. Background Art

[0002] The three highs are a general term for high blood lipids, high blood pressure, and high blood sugar.

[0003] Hypertension refers to a condition characterized by an increase in systemic arterial blood pressure (systolic and / or diastolic blood pressure) (systolic blood pressure ≥ 140 mmHg, diastolic blood pressure ≥ 90 mmHg). Because it occurs unconsciously, it is called a "silent killer" and may be accompanied by a clinical syndrome of functional or organic damage to organs such as the heart, brain, and kidney. Hypertension is the most common chronic disease and the most important risk factor for cardiovascular and cerebrovascular diseases. The blood pressure of a normal person fluctuates within a certain range with changes in the internal and external environment. In the overall population, blood pressure levels gradually increase with age, with systolic blood pressure being more obvious, but diastolic blood pressure after the age of 50 shows a downward trend, and pulse pressure also increases accordingly. In recent years, people have a deeper understanding of the role of multiple risk factors for cardiovascular disease and the protection of target organs such as the heart, brain, and kidney. The diagnostic criteria for hypertension are also constantly adjusted. It is believed that patients with the same blood pressure level have different risks of cardiovascular disease. Therefore, the concept of blood pressure stratification has been developed, that is, patients with different risks of cardiovascular disease should have different appropriate blood pressure levels. Blood pressure values ​​and risk factor assessment are the main basis for diagnosing and formulating hypertension treatment plans. The goals of hypertension management are different for different patients. When facing patients, doctors judge the most appropriate blood pressure range for the patient based on the reference standards and their specific circumstances, and adopt targeted treatment measures.

[0006] Hyperlipidemia refers to high cholesterol or triglycerides or low high-density lipoprotein cholesterol in the blood, which is called dyslipidemia in modern medicine. It is the main factor leading to atherosclerosis and a risk factor for the development of cardiovascular and cerebrovascular diseases. It is insidious and most of the time there are no clinical symptoms, so it is called the "invisible killer."

[0007] There is no unified method for the diagnosis of hyperlipidemia internationally or domestically. In the past, it was believed that a plasma total cholesterol concentration >5.17mmol / L (200mg / dl) could be defined as hypercholesterolemia, and a plasma triglyceride concentration >2.3mmol / L (200mg / dl) was defined as hypertriglyceridemia. Due to factors such as the different populations tested and the differences in the test methods used, the diagnostic criteria for hyperlipidemia are different in different places. However, in order to prevent and treat atherosclerosis and coronary heart disease, the appropriate plasma cholesterol level should be determined based on the patient's future risk of cardiovascular and cerebrovascular diseases. The higher the risk, the lower the appropriate plasma cholesterol level should be.

[0008] The new standard recommends starting drug treatment when the LDL-C concentration is >130mg / dl, with the LDL-C concentration <100mg / dl as the treatment target. If the risk of cardiovascular and cerebrovascular diseases in the future is very high, drug treatment should be started earlier and more stringent treatment targets should be adopted. Low HDL-C concentration is a risk factor for coronary heart disease, which is <40mg / dl. Hyperlipidemia can be divided into primary and secondary types. Primary is related to congenital and genetic factors, and is caused by single gene defects or multiple gene defects, which cause abnormal receptors, enzymes or apolipoproteins involved in lipoprotein transport and metabolism, or due to environmental factors (diet, nutrition, drugs) and unknown mechanisms. Secondary hyperlipidemia often occurs in metabolic disorders (diabetes, hypertension, myxedema, hypothyroidism, obesity, liver and kidney diseases, hyperadrenocortical function), or is related to other factors such as age, gender, season, drinking, smoking, diet, physical activity, mental stress, emotional activity, etc.

[0009] Hyperglycemia is when the glucose content in the body's blood is higher than normal. It is an independent pathological change in the body. The lesion site is in the blood, and the nature of the lesion is a disorder of blood sugar metabolism. When the blood sugar value is higher than the normal range, it is called hyperglycemia. The normal value of fasting blood sugar is below 6.1mmol / L, and the normal value of blood sugar two hours after a meal is below 7.8mmol / L. If it is higher than this range, it is called hyperglycemia.

[0010] Under normal circumstances, the human body can ensure the balance between the source and destination of blood sugar through hormone regulation and neural regulation, so that blood sugar is maintained at a certain level. However, under the combined effects of genetic factors (such as a family history of diabetes) and environmental factors (such as an unreasonable diet, obesity, etc.), the two major regulatory functions are disrupted, and blood sugar levels will rise. The clinical manifestations of hyperglycemia can have obvious symptoms, such as dry mouth, frequent drinking, frequent urination, and weight loss; or they can be latent symptoms without obvious subjective discomfort.

[0011] Increased blood sugar and increased urine sugar can trigger osmotic diuresis, which can cause symptoms of polyuria; increased blood sugar and large amounts of water loss will increase blood osmotic pressure accordingly, and high blood osmotic pressure can stimulate the thirst center of the hypothalamus, causing symptoms of thirst and polydipsia; due to relative or absolute lack of insulin, glucose in the body cannot be utilized, and protein and fat consumption increase, causing fatigue and weight loss; in order to compensate for the lost sugar and maintain body activity, more food is needed; this forms the typical "three more and one less" symptom. The symptoms of polydipsia and polyuria in diabetic patients are proportional to the severity of the disease. In addition, it is worth noting that the more the patient eats, the higher the blood sugar, the more sugar is lost in the urine, and the more hungry he is, which eventually leads to a vicious cycle.

[0012] "Three more and one less" is the most common clinical manifestation of diabetes, namely polydipsia, polyphagia, polyuria and weight loss. However, at present, some diabetic patients in clinical practice have delayed diagnosis because they do not have typical "three more and one less" symptoms. Some patients with type 2 diabetes do not have obvious "three more and one less" symptoms. On the one hand, many diabetic patients are found through physical examinations that their blood sugar is only slightly elevated, and has not exceeded or only slightly exceeded the renal glucose threshold. The renal sugar excretion is very small, which is not enough to cause obvious "three more and one less" symptoms. On the other hand, it is caused by the increase in the renal glucose threshold of some patients. Under normal circumstances, all glucose is reabsorbed in the kidneys, and no sugar is excreted in the urine; if blood sugar rises slightly, the renal tubular reabsorption value will increase accordingly; but when blood sugar rises to a certain level, exceeding the maximum reabsorption range of the renal tubules, some glucose will be excreted from the urine, thus forming diabetes. This critical blood sugar value is the renal glucose threshold, and the normal value is 8.8-9.9mmol / L. Kidney disease and some other urinary system diseases will have different degrees of impact on it, and the renal glucose threshold of most elderly patients will also increase accordingly. After the renal glucose threshold increases, patients will not have diabetes even if their blood sugar is very high. Without diabetes, osmotic diuresis will not be triggered, so there will be no polyuria symptoms; without losing a lot of water, the plasma osmotic pressure will change less, and the stimulation to the hypothalamic center will be reduced accordingly, so there will be no thirst and polydipsia symptoms; without diabetes, a large amount of sugar will not be lost, so there will be no obvious hunger, and the symptoms of polyphagia will be reduced; without diabetes, the body's nutrient loss will be reduced, so the degree of weight loss can be reduced accordingly. As a result, these diabetic patients can eventually avoid the symptoms of "three more and one less".

[0013] Immunity is the body's own defense mechanism, which is the body's ability to identify and eliminate any foreign invading substances (viruses, bacteria, etc.); to deal with aging, damaged, dead, and degenerated cells of the body; and to identify and deal with mutated cells and virus-infected cells in the body.

[0014] Modern immunology believes that immunity is the physiological reaction of the human body to identify and exclude "foreign" things. The immune system is the one that performs this function in the human body. For millions of years, humans have lived in an environment that is both suitable for survival and full of dangers. Humans have survived and have also acquired extraordinary immunity. Therefore, immunity is the product of the biological evolution process.

[0018] Immunity can be divided into nonspecific immunity and specific immunity. Immunity can be divided into the following according to the different ways in which it is acquired:

[0019] 1. Innate immunity is something that people have from birth. For example, swine fever spreads quickly among pigs, but it has nothing to do with humans. This is because humans are naturally immune to this disease;

[0020] 2. Acquired immunity is naturally acquired in the course of life after birth.

[0021] A body with low immunity is more susceptible to infection or cancer. An abnormally strong immunity can also have harmful consequences on the body, such as allergic reactions and autoimmune diseases. For various reasons, the immune system cannot function properly, and in this case, it is very easy to be infected by bacteria, viruses, fungi, etc.

[0022] Therefore, the most direct manifestation of low immunity is susceptibility to illness. Frequent illness increases the body's consumption, so people generally have symptoms such as physical weakness, malnutrition, mental depression, fatigue, decreased appetite, and sleep disorders. Getting sick, taking injections, and taking medicine have become commonplace. It takes a long time to recover from each illness, and the illness often recurs. In the long run, it will lead to physical and intellectual stunting, and it is also easy to induce major diseases.

[0023] The underlying reason is low immunity or incomplete immunity. When the human immune function is disordered or the immune system is incomplete, the following problems will recur: repeated colds, repeated tonsillitis, repeated asthma, repeated bronchitis, repeated pneumonia, and repeated diarrhea, so they must not be underestimated.

[0024] People with three highs are sometimes also referred to as people with underlying diseases. Their immunity is at a low level.

[0025] The following reasons may be responsible for the low immunity of people with three highs: 1. Chronic inflammation: patients with three highs are often accompanied by chronic inflammation. Chronic inflammation will offset the immune system's ability to respond, resulting in an untimely or inadequate immune system response. 2. Impaired immune cell function: patients with three highs may have a large number of inflammatory substances in their bodies, such as glycation products, lipid peroxides, etc. These substances will cause damage to immune cell function, leading to decreased immunity. 3. Cognitive stress: Being in a busy and stressful working state for a long time will cause excessive cognitive stress in patients with three highs, which will affect the normal regulation of human functions and also lead to decreased immunity. 4. Poor diet: patients with three highs often have a high-fat, high-salt diet, lack of dietary fiber, vitamins and other nutrients, resulting in a decrease in the support for the immune system. 5. Bad living habits: bad living habits such as smoking and drinking will also lead to a decrease in the immunity of patients with three highs.

[0026] Why do patients with three highs need to enhance their immunity? Patients with hypertension, hyperlipidemia and diabetes have varying degrees of metabolic disorders, often with multiple complications, decreased defense function, increased susceptibility to various infections, and are prone to viral infections, especially upper respiratory tract infections, which have the highest incidence. Due to lipid metabolism and sugar metabolism disorders, a good breeding ground is provided for the growth of bacteria and viruses. Once the immune function is reduced, pathogenic bacteria will take advantage of the situation and multiply uncontrollably, increasing the risk of infection. The infection is more serious and dangerous, and the prognosis is extremely poor. Infection can cause blood sugar, blood pressure and blood lipids to rise, making it difficult to control these indicators, putting the body in a state of stress and aggravating the damage to the target organs. Conversely, poor control of indicators will also aggravate the infection, thus entering a vicious circle.

[0027] The market prospects for dietary supplements that prevent the three highs, tumors, obesity and enhance immunity are broad.

[0028] Therefore, it is imperative to develop and optimize new raw materials and formulas for dietary supplements that lower the three highs, and to research and develop new dietary supplements that lower the three highs.

[0029] In view of this, the present invention provides a nutritional supplement for preventing three highs, tumors, obesity and improving immunity, and a tablet and preparation method thereof. Summary of the invention

[0030] The object of the present invention is to provide a nutritional supplement for preventing three highs, tumors, obesity and improving immunity, and a tablet thereof and a preparation method thereof.

[0031] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are particularly adopted:

[0032] In a first aspect, the present invention provides a nutritional supplement for preventing three highs, tumors, obesity and improving immunity. The raw materials of the nutritional supplement for preventing three highs, tumors, obesity and improving immunity include protein core chlorella, oligoxylose, yeast β-glucan, and tea theanine.

[0033] Furthermore, in a preferred embodiment of the present invention, the raw materials of the nutritional supplement for preventing the three highs, tumors, obesity and improving immunity include 7500-8500 parts of pyrenoid algae, 500-1500 parts of xylo-oligosaccharides, 300-700 parts of yeast β-glucan, and 300-700 parts of tea theanine, measured by weight.

[0034] Furthermore, in a preferred embodiment of the present invention, 7800-8200 parts of Chlorella pyrenoidosa, 800-1200 parts of xylo-oligosaccharides, 400-600 parts of yeast β-glucan, and 400-600 parts of tea theanine are included.

[0035] Furthermore, in a preferred embodiment of the present invention, 8000 parts of Chlorella pyrenoidosa, 1000 parts of xylo-oligosaccharides, 500 parts of yeast β-glucan, and 500 parts of tea theanine are included.

[0036] In a second aspect, the present invention also provides a method for preparing tablets of nutritional supplements for preventing three highs, tumors, obesity and improving immunity.

[0037] The raw materials are dried at 38-42°C for 25-35 minutes, taken out and sieved through a 100-mesh sieve, mixed evenly, made into a soft material, sieved through a 12-14 mesh sieve to make wet granules, the granules are dried at 55-65°C for 50-60 minutes, sieved through a 16-18 mesh sieve to size the granules, 0.5% magnesium stearate is added to the granules, mixed evenly, and pressed into tablets.

[0038] Furthermore, in a preferred embodiment of the present invention, the raw material is dried in an oven at 40° C. for 30 min.

[0039] Furthermore, in a preferred embodiment of the present invention, the particles are dried at 60° C. for 55 min.

[0040] Furthermore, in a preferred embodiment of the present invention, the soft material is passed through a 12-mesh sieve to prepare wet granules, and then passed through a 16-mesh sieve to prepare whole granules.

[0041] In a third aspect, the present invention also provides a use of the nutritional supplement or the nutritional supplement tablet in the preparation of food or health food for preventing the three highs and / or tumors and / or obesity, and / or improving immunity.

[0042] Compared with the prior art, the beneficial effects of the present invention include:

[0043] Nutritional supplements that prevent the three highs, tumors, obesity and improve immunity have significantly improved the indicators of preventing the three highs, tumors, obesity and improving immunity compared with existing technologies. DETAILED DESCRIPTION

[0044] The embodiments of the present invention will be described in detail below in conjunction with the examples, but it will be appreciated by those skilled in the art that the following examples are only used to illustrate the present invention and should not be construed as limiting the scope of the present invention. If specific conditions are not specified in the examples, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0045] The features and properties of the present invention are further described in detail below in conjunction with the embodiments:

[0046] Example 1

[0047] Take 7500 grams of Chlorella pyrenoidosa, 1500 grams of xylo-oligosaccharide, 700 grams of yeast β-glucan, and 300 grams of tea theanine.

[0048] The raw materials were dried at 38°C for 35 min, taken out and sieved through a 100-mesh sieve, mixed evenly, made into a soft material, sieved through a 12-mesh sieve to make wet granules, the granules were dried at 65°C for 50 min, sieved through an 18-mesh sieve to size the granules, 0.5% magnesium stearate was added to the granules, mixed evenly, and tablets were obtained.

[0049] Example 2

[0050] Take 8500 grams of Chlorella pyrenoidosa, 500 grams of xylo-oligosaccharide, 300 grams of yeast β-glucan, and 700 grams of tea theanine.

[0051] The raw materials were dried at 42°C for 25 min, taken out and sieved through a 100-mesh sieve, mixed evenly, made into a soft material, sieved through a 14-mesh sieve to make wet granules, the granules were dried at 55°C for 60 min, sieved through a 16-mesh sieve to size the granules, 0.5% magnesium stearate was added to the granules, mixed evenly, and pressed into tablets.

[0052] Example 3

[0053] Take 7800 grams of Chlorella pyrenoidosa, 1200 grams of xylo-oligosaccharide, 600 grams of yeast β-glucan, and 400 grams of tea theanine.

[0054] The raw materials are dried at 40°C for 25-35 minutes, taken out and sieved through a 100-mesh sieve, mixed evenly, made into a soft material, sieved through a 12-mesh sieve to make wet granules, the granules are dried at 60°C for 55 minutes, sieved through a 16-mesh sieve to size the granules, 0.5% magnesium stearate is added to the granules, mixed evenly, and pressed into tablets.

[0055] Example 4

[0056] Take 8200 grams of Chlorella pyrenoidosa, 800 grams of xylo-oligosaccharide, 400 grams of yeast β-glucan, and 600 grams of tea theanine.

[0057] The tablet pressing method is the same as in Example 3.

[0058] Example 5

[0059] Take 8000 grams of Chlorella Vulgaris, 1000 grams of xylo-oligosaccharide, 500 grams of yeast β-glucan, and 500 grams of tea theanine.

[0060] The tablet pressing method is the same as in Example 3.

[0061] Comparative Example 1

[0062] Take 8000 grams of Chlorella vulgaris and add starch to make the total amount of raw materials 10000 grams.

[0063] The tablet pressing method is the same as in Example 3.

[0064] Comparative Example 2

[0065] Take 1000 grams of xylo-oligosaccharide, 500 grams of yeast β-glucan, 500 grams of tea theanine, and add starch to make the total amount of raw materials 10000 grams.

[0066] The tablet pressing method is the same as in Example 3.

[0067] Comparative Example 3

[0068] Take 1000 grams of xylooligosaccharide and add starch to make the total amount of raw materials 10000 grams.

[0069] The tablet pressing method is the same as in Example 3.

[0070] Comparative Example 4

[0071] Take 8000 grams of Chlorella pyrenoidosa, 500 grams of yeast β-glucan, 500 grams of tea theanine, and add starch to make the total amount of raw materials 10000 grams.

[0072] The tablet pressing method is the same as in Example 3.

[0073] Comparative Example 5

[0074] Take 500 grams of yeast β-glucan and add starch to make the total amount of raw materials 10,000 grams.

[0075] The tablet pressing method is the same as in Example 3.

[0076] Comparative Example 6

[0077] Take 8000 grams of Chlorella pyrenoidosa, 1000 grams of xylo-oligosaccharide, 500 grams of tea theanine, and add starch to make the total amount of raw materials 10000 grams.

[0078] The tablet pressing method is the same as in Example 3.

[0079] Comparative Example 7

[0080] Take 8000 grams of Chlorella Vulgaris, 1000 grams of arabinose, 500 grams of yeast β-glucan, and 500 grams of tea theanine.

[0081] The tablet pressing method is the same as in Example 3.

[0082] Beneficial effects of the present invention:

[0083] 1. Lowering blood lipids and body weight

[0084] (1) Methods

[0085] Male Wistar rats, weighing 180-220g, were randomly divided into a normal control group, a model group, Example 1-5 groups and Comparative Example 1-7 groups, with 10 rats in each group. During the experiment, the rats were free to eat ordinary feed and drink water. Every morning, Example 1-5 groups and Comparative Example 1-7 groups were gavaged with the corresponding samples (0.8g / kg·d), and the other groups were gavaged with starch (0.8g / kg·d); in the afternoon, the normal control group was gavaged with an equal volume of Tween 80 and propylene glycol mixture (volume fraction 1:1), and the other groups were gavaged with high-fat emulsion (25% lard, 12% cholesterol, 2% sodium cholate, 1% propylthiouracil, 20% Tween 80, 20% propylene glycol, 10% 10% sodium deoxycholate aqueous solution and 10% water), and the gavage dose was 10mL·kg -1 The experimental period was 35 days. After the last administration, the rats were fasted but not watered for 12 hours. After the body weight was measured, the eyeballs were removed to collect blood, which was centrifuged to obtain serum. The total cholesterol (TC), triglyceride (TG), and high-density lipoprotein cholesterol (HDL-C) in the serum were measured. See Table 1.

[0086] (2) Blood lipid and body weight test results (see Table 1)

[0087] Table 1

[0088] Group TC(mmol / L) TG (mmol / L) HDL-C (mmol / L) Weight gain (g) Normal control group 1.35±0.26 0.68±0.15 1.19±0.16 2.6±1.1 Model Group 2.48±0.55 1.40±0.28 0.80±0.09 43.8±5.9 Example 1 1.83±0.36 0.93±0.19 1.00±0.11 9.7±1.8 Example 2 1.80±0.33 0.95±0.18 0.98±0.14 9.8±2.0 Example 3 1.69±0.31 0.89±0.15 1.04±0.12 8.6±1.9 Example 4 1.75±0.32 0.88±0.17 1.05±0.14 8.0±2.1 Example 5 1.62±0.29 0.85±0.14 1.08±0.13 7.5±1.8 Comparative Example 1 2.13±0.48 1.19±0.23 0.93±0.12 19.0±2.8 Comparative Example 2 2.35±0.55 1.29±0.28 0.85±0.13 36.7±4.2 Comparative Example 3 2.26±0.54 1.31±0.29 0.88±0.11 33.9±3.8 Comparative Example 4 2.09±0.50 1.14±0.22 0.95±0.14 20.2±3.0 Comparative Example 5 2.18±0.52 1.17±0.20 0.92±0.12 28.7±3.5 Comparative Example 6 2.31±0.57 1.28±0.26 0.90±0.10 35.2±4.0 Comparative Example 7 1.97±0.44 1.02±0.20 0.95±0.12 17.2±2.3

[0089] 2. Blood pressure reduction

[0090] (1) Methods

[0091] Spontaneously hypertensive rats were randomly divided into a model group, Example 1-5 groups and Comparative Example 1-7 groups, with 10 rats in each group, half of which were male and half were female. The Example 1-5 groups and Comparative Example 1-7 groups were gavaged with corresponding samples (Example 1-5 and Comparative Example 1-7 samples) at a gavage volume of 0.8 g / kg·d (the model group was gavaged with the same amount of starch) for 5 consecutive weeks, during which they were kept normally.

[0092] (2) Blood pressure test results (see Table 2)

[0093] After 5 weeks, the spontaneously hypertensive rats were fixed in a 38°C incubator and preheated for 10 min, and the blood pressure was measured by the tail pulse method.

[0094] Table 2

[0095] Group blood pressure Model Group 185.2±9.4 Example 1 171.9±8.1 Example 2 172.2±8.3 Example 3 169.3±7.5 Example 4 168.0±7.0 Example 5 165.3±6.7 Comparative Example 1 183.5±9.2 Comparative Example 2 176.1±8.9 Comparative Example 3 178.0±8.7 Comparative Example 4 182.5±9.1 Comparative Example 5 179.8±9.0 Comparative Example 6 177.9±8.5 Comparative Example 7 173.8±9.1

[0096] 3. Blood sugar reduction

[0097] (1) Methods

[0098] The mice were randomly divided into a normal control group, a model group, groups 1 to 5 of embodiments and groups 1 to 7 of comparative examples, with 10 mice in each group. Except for the normal control group, which was intervened with physiological saline, the other groups were induced with streptozotocin (STZ) to establish a diabetes model, using a mass fraction of 4 mg / L sodium citrate-STZ solution, and intraperitoneally injected at a dose of 40 mg / kg, once a day, for 5 consecutive days. On the second day, groups 1 to 5 of embodiments and groups 1 to 7 of comparative examples were gavaged with corresponding samples (samples of embodiments 1 to 5 and comparative examples 1 to 7), with a gavage volume of 0.8 g / kg·d (the normal control group and the model group were gavaged with the same amount of starch), for 5 consecutive weeks.

[0099] (2) Blood sugar test results (see Table 3)

[0100] After 5 weeks, the fasting blood glucose of the mice was measured.

[0101] Table 3

[0102]

[0103]

[0104] 4. Survival of glioma-bearing mice

[0105] (1) Methods

[0106] 6-week-old Balb / c male nude mice were raised normally in an SPF environment with a room temperature of 27-32°C, sufficient exercise space and 12h light exposure time. The mice were randomly divided into a normal control group, a model group, Example 1-5 groups and Comparative Example 1-7 groups, with 10 mice in each group. Example 1-5 groups and Comparative Example 1-7 groups were gavaged with corresponding samples (Example 1-5 and Comparative Example 1-7 samples) at a gavage volume of 0.8g / kg·d (the normal control group and the model group were gavaged with the same amount of starch) for 20 consecutive days. On the 21st day, except for the normal control group which was intervened with normal saline, the other mice were intraperitoneally anesthetized, and U251 cells (glioma cells) were digested and collected at 2x10 6 Each was dissolved in 0.1 ml PBS and injected subcutaneously into the right armpit of nude mice. Groups 1 to 5 of Examples and groups 1 to 7 of Comparative Examples continued to be gavaged with the corresponding samples, and the normal control group and model group were gavaged with the same amount of starch. After continuous administration for 30 days, the number of surviving mice was counted (see Table 4).

[0107] (2) Results

[0108] Table 4

[0109]

[0110]

[0111] 5. Improve immunity

[0112] (1) Methods

[0113] Take RAW264.7 cells in the logarithmic growth phase and adjust the cell concentration to 5x10 5 100uL / well was inoculated in a 96-well culture plate, and cultured for 2h at 37°C, 5% CO2 in a constant temperature incubator. When the cells adhered to the wall, the supernatant was removed, and 100uL of 80g / mL samples of Examples 1 to 5 and Comparative Examples 1 to 7 (prepared with high-glucose DMEM culture medium containing 10% fetal bovine serum) were added to each well. The blank control group was added with 100uL of culture solution, and 5 replicates were set for each group. After continuing to culture for 24h in a constant temperature incubator at 37°C, 5% CO2, 10uL of 5mg / mL of MTT working solution was added, and culture was continued for 4h. The culture medium was discarded, and after carefully flushing PBS 2 to 3 times, 150uL of DMSO dissolving solution was added to each well, and the formazan was thoroughly dissolved. The OD value at 570nm was measured by an ELISA instrument, and the RAW264.7 cell proliferation rate was calculated according to the following formula.

[0114] Proliferation rate (%) = sample OD 570nm ÷blank control group OD 570nm x100

[0115] (2) Results of the experiment to improve immunity (see Table 5)

[0116] Table 5 Effects on the proliferation activity of RAW264.7 cells

[0117]

[0118]

[0119] In summary, nutritional supplements for preventing the three highs, tumors, obesity and improving immunity have shown significant improvements in the indicators of preventing the three highs, tumors, obesity and improving immunity compared with the control group.

[0120] The links not described in detail in the present invention are all common knowledge that can be selected by ordinary technicians in the field. Although the present invention has been described in detail with general descriptions and specific implementation schemes above, it is obvious to ordinary technicians in the field that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all belong to the scope of protection claimed by the present invention.

Claims

1. A nutritional supplement for preventing three highs, tumors, obesity and improving immunity, characterized in that: The raw materials of the nutritional supplement for preventing three highs, tumors, obesity and improving immunity include pyrenoid chlorella, oligoxylose, yeast beta-glucan and tea theanine.

2. The nutritional supplement for preventing three highs, tumors, obesity and improving immunity according to claim 1, characterized in that: Measured by weight, the raw materials of the nutritional supplement for preventing the three highs, tumors, obesity and improving immunity include 7500-8500 parts of the pyrenoid chlorella, 500-1500 parts of the xylo-oligosaccharides, 300-700 parts of the yeast β-glucan, and 300-700 parts of theanine from tea.

3. The nutritional supplement for preventing three highs, tumors, obesity and improving immunity according to claim 1, characterized in that: Calculated by weight, the raw materials of the nutritional supplement for preventing the three highs, tumors, obesity and improving immunity include 7800-8200 parts of the pyrenoid chlorella, 800-1200 parts of the xylo-oligosaccharides, 400-600 parts of the yeast β-glucan, and 400-600 parts of theanine from tea.

4. The nutritional supplement for preventing three highs, tumors, obesity and improving immunity according to claim 1, characterized in that: Measured by weight, the raw materials of the nutritional supplement for preventing the three highs, tumors, obesity and improving immunity include 8000 parts of the pyrenoid chlorella, 1000 parts of the xylo-oligosaccharides, 500 parts of the yeast β-glucan and 500 parts of theanine from tea.

5. A nutritional supplement according to claim 1, characterized in that: The nutritional supplement is in the form of tablets.

6. A method for preparing tablets of nutritional supplements for preventing three highs, tumors, obesity and improving immunity according to claims 1 to 4, characterized in that: The raw materials are dried at 38-42° C. for 25-35 minutes, taken out and sieved through a 100-mesh sieve, mixed evenly to prepare a soft material, sieved through a 12-14-mesh sieve to prepare wet granules, the granules are dried at 55-65° C. for 50-60 minutes, sieved through a 16-18-mesh sieve to prepare granules, 0.5% magnesium stearate is added to the granules, mixed evenly and pressed into tablets.

7. A method for preparing tablets of a nutritional supplement for preventing three highs, tumors, obesity and improving immunity according to claim 6, characterized in that: The raw materials were dried in an oven at 40°C for 30 min.

8. A method for preparing tablets of a nutritional supplement for preventing three highs, tumors, obesity and improving immunity according to claim 6, characterized in that: The granules were dried at 60°C for 55 min.

9. A method for preparing tablets of a nutritional supplement for preventing three highs, tumors, obesity and improving immunity according to claim 6, characterized in that: The soft material is passed through a 12-mesh sieve to prepare wet granules, and then passed through a 16-mesh sieve to prepare whole granules.

10. Use of the nutritional supplement according to any one of claims 1 to 4 or the nutritional supplement tablet according to any one of claims 5 to 9 in the preparation of food or health food for preventing three highs and / or tumors and / or obesity and / or improving immunity.

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