Process for the production of polyester fiber cloth by slit method and polyester fiber cloth and use thereof

Abstract

The present application relates to the technical field of polyester fiber cloth, and discloses a method for preparing polyester fiber cloth by a slit method, polyester fiber cloth and application thereof, the method comprising: (1) sending the molten core layer material and skin layer material into a spinneret to perform spinning to obtain a filamentous melt with a skin-core structure; (2) cooling the filamentous melt with the skin-core structure to obtain a nascent fiber; (3) stretching the nascent fiber in a slit to obtain polyester fiber with a skin-core structure; and (4) taking polyester fiber with an average diameter of 18-30 microns and a diameter standard deviation of less than or equal to 2.2 microns to perform webbing and hot pressing to obtain the polyester fiber cloth. When the fiber cloth prepared by the method of the present application is used for cell culture, the sugar metabolism capacity of the cells can be significantly improved, and the cell survival rate can be improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of polyester fiber cloth, in particular to a method for preparing polyester fiber cloth by slit method, polyester fiber cloth and application thereof. BACKGROUND

[0002] At present, large-scale animal cell culture technology has been widely used in the production of various cells and cell products, including enzymes, growth factors, vaccines and antibodies and other biological products. Most animal cells have the habit of adherent growth, so when cultured in vitro, they usually need to provide a support for their growth, which is called a cell culture carrier by those skilled in the art. At present, the commonly used cell culture carriers in China are granular carriers (diameter 2-11mm), porous microsphere carriers (diameter <1mm) or sheet-shaped fiber cloth carriers. However, the granular carrier has the problem that cells can only adhere to the surface and the area-to-volume ratio is small, while the porous microsphere carrier has the problem that the voids are easily blocked and further affect the exchange of oxygen and substances. The sheet-shaped fiber cloth carrier can overcome the above problems and has high culture efficiency, but there are relatively few related researches at home and abroad at present. The existing ones mainly use electrospinning scheme and are mainly used in tissue engineering in vivo transplantation, and are rarely used for in vitro large-scale cell culture, because the overall culture effect is not good enough. SUMMARY

[0003] The purpose of the present application is to overcome the above-mentioned problems of the prior art and provide a method for preparing polyester fiber cloth by slit method, polyester fiber cloth and application thereof.

[0004] In order to achieve the above-mentioned purpose, the first aspect of the present application provides a method for preparing polyester fiber cloth, which comprises:

[0005] (1) feeding the molten core layer material and the skin layer material into a spinneret for spinning to obtain a filamentous melt with a skin-core structure;

[0006] (2) cooling the filamentous melt with a skin-core structure to obtain a primary fiber;

[0007] (3) stretching the primary fiber in a slit to obtain a polyester fiber with a skin-core structure;

[0008] (4) taking the polyester fiber with an average diameter of 17-32μm and a diameter standard deviation of ≤2.2μm for laying and hot pressing to obtain a polyester fiber cloth.

[0009] The second aspect of the present application provides a polyester fiber cloth prepared by the above-mentioned method.

[0010] The third aspect of the present application provides a polyester fiber cloth, which is self-adhered by polyester fibers with a sheath-core structure, the thickness of the polyester fiber cloth is 0.4-0.5mm, the area density is 80-130g / m 2 ; wherein the average diameter of the polyester fibers is 17-32μm, and the diameter standard deviation is ≤2.2μm.

[0011] The fourth aspect of the present application provides the polyester fiber cloth as described above for use in cell culture.

[0012] When the fiber cloth prepared by the method of the present application is used for cell culture, the sugar metabolism capacity of the cells can be significantly improved, and the higher the sugar metabolism capacity is, the higher the cell proliferation rate and survival rate are (only living cells can metabolize sugar, and dead cells cannot metabolize sugar), that is, the composite polyester fibers can achieve better results when used for cell culture. DETAILED DESCRIPTION

[0013] The endpoints of the ranges and any values disclosed herein are not limited to the precise values recited as the exact dimensions are not critical to the present application. The endpoints of the ranges and any values are provided as approximations only, and are understood to be open-ended. Thus, the endpoints of the ranges and any values are not to be understood as being stated to be the only values that can be used to define the ranges and any values. The range and any value is understood to encompass values approximating the value, as well as values that are substantially the same as the value.

[0014] The first aspect of the present application provides a method for preparing a polyester fiber cloth, the method comprising:

[0015] (1) feeding the molten core layer material and the sheath layer material into a spinneret for spinning to obtain a filamentous melt with a sheath-core structure;

[0016] (2) cooling the filamentous melt with a sheath-core structure to obtain a nascent fiber;

[0017] (3) stretching the nascent fiber in a slit to obtain a polyester fiber with a sheath-core structure;

[0018] (4) taking the polyester fibers with an average diameter of 17-32μm and a diameter standard deviation of ≤2.2μm for laying and hot pressing to obtain the polyester fiber cloth.

[0019] According to the present application, preferably, the average diameter of the polyester fiber is 17-32 μm, and the standard deviation of the diameter is ≤2.2 μm. In the present application, the average diameter of the polyester fiber can be 17 μm, 18 μm, 20 μm, 22 μm, 24 μm, 26 μm, 28 μm, 30 μm, 32 μm, and a range formed by any two of the above. The standard deviation of the diameter of the polyester fiber can be 2.2 μm, 2 μm, 1.8 μm, 1.6 μm, 1.4 μm, 1.2 μm, 1 μm, 0.8 μm, 0.6 μm, 0.4 μm, 0.2 μm, 0.1 μm, and a range formed by any two of the above, for example, 0.1-2.2 μm.

[0020] In the present application, the test method of the standard deviation of the diameter is as follows: 50 polyester fibers are randomly taken, a small section of each is randomly cut, a scanning electron microscope (SEM) photo is taken, and the diameter of each fiber is measured from the photo with an accuracy of 0.1 μm, and then the standard deviation of the diameter values is calculated according to the calculation method of the standard deviation.

[0021] In the present application, the polyester fiber refers to a polyester fiber with a length of more than 150 mm.

[0022] According to the present application, the melting point of the core layer material is higher than that of the skin layer material by 20℃ or more (for example, 20℃, 22℃, 24℃, 25℃, 30℃, 40℃, 50℃, 60℃, 80℃, 100℃, 110℃, 120℃, 130℃, 140℃, 150℃, 160℃, and a range formed by any two of the above, for example, 20-160℃), preferably, the melting point of the core layer material is different from that of the skin layer material by 24℃ or more.

[0023] According to the present application, preferably, the intrinsic viscosity of the skin layer material is higher than that of the core layer material by 0.01-0.18 dL / g, and the standard deviation of the intrinsic viscosity of the core layer material and the skin layer material is ≤0.015 dL / g.

[0024] In the present application, the test method of the intrinsic viscosity of the polyester is the test method of the intrinsic viscosity described in GBT14190-2017. Among them, the test method of the standard deviation of the intrinsic viscosity is as follows: 6 samples are randomly taken, the intrinsic viscosity is tested according to the test method of the intrinsic viscosity described in GBT14190-2017, and the standard deviation of the values is taken. The smaller the standard deviation of the intrinsic viscosity, the higher the uniformity of the intrinsic viscosity of the same batch of materials.

[0025] According to the present application, the standard deviation of the intrinsic viscosity of the core material and the sheath material can be independently 0.015 dL / g, 0.01 dL / g, 0.009 dL / g, 0.008 dL / g, 0.007 dL / g, 0.006 dL / g, 0.005 dL / g, 0.004 dL / g, 0.003 dL / g, 0.002 dL / g, 0.001 dL / g, 0.0001 dL / g, and a range between any two of the above, for example, 0.0001-0.015 dL / g.

[0026] According to the present application, preferably, the shape of the orifice of the spinneret is concentric circle, the difference between the maximum diameter and the minimum diameter of each inner circle of the orifice is ≤2 μm, and the difference between the maximum diameter and the minimum diameter of each outer circle of the orifice is ≤2 μm. The difference between the maximum diameter and the minimum diameter of each inner circle or each outer circle can be independently 0.1 μm, 0.5 μm, 1.5 μm, 2 μm, and a range between any two of the above, for example, 0.1-2 μm.

[0027] In the present application, it can be understood that the core material forms the core structure of the polyester fiber after passing through the inner circle of the orifice, and the sheath material forms the sheath structure of the polyester fiber after passing through the outer circle of the orifice, and then the polyester fiber with the sheath-core structure is formed after cooling and stretching.

[0028] According to the present application, preferably, the inner diameter of the inner circle of the orifice is 15-25 μm, and the inner diameter of the outer circle of the orifice is 25-35 μm. In the present application, the accurate diameter of the spinneret is measured by a profilometer.

[0029] According to the present application, preferably, the core material is melted at 220-280 ℃ to obtain the molten core material.

[0030] According to the present application, preferably, the sheath material is melted at 200-280 ℃ to obtain the molten sheath material.

[0031] In the present application, it can be understood that the temperature of the core material melt and the sheath material melt refers to the temperature of the spinneret when it is extruded from the outlet of the spinneret.

[0032] According to the present application, preferably, the core material is homopolymerized polyethylene terephthalate.

[0033] According to the present application, preferably, the homopolyethylene terephthalate has an intrinsic viscosity of 0.6-0.8 dL / g (e.g., 0.6 dL / g, 0.64 dL / g, 0.66 dL / g, 0.68 dL / g, 0.70 dL / g, 0.72 dL / g, 0.74 dL / g, 0.76 dL / g, 0.78 dL / g, 0.8 dL / g, and ranges formed by any two of the aforementioned values), further preferably 0.65-0.7 dL / g, and a standard deviation of the intrinsic viscosity of <0.015 dL / g.

[0034] According to the present application, preferably, the homopolyethylene terephthalate has a melting point of 240°C or higher (e.g., 240°C, 245°C, 248°C, 250°C, 255°C, 260°C, 265°C, 270°C, 280°C, 290°C, 300°C, 320°C, 350°C, 380°C, and ranges formed by any two of the aforementioned values), more preferably 245-265°C.

[0035] According to the present application, preferably, the skin material is a copolyethylene terephthalate.

[0036] According to the present application, preferably, the copolyethylene terephthalate has an intrinsic viscosity of 0.5-0.9 dL / g (e.g., 0.5 dL / g, 0.52 dL / g, 0.54 dL / g, 0.60 dL / g, 0.62 dL / g, 0.64 dL / g, 0.66 dL / g, 0.68 dL / g, 0.70 dL / g, 0.72 dL / g, 0.74 dL / g, 0.76 dL / g, 0.78 dL / g, 0.80 dL / g, 0.82 dL / g, 0.84 dL / g, 0.86 dL / g, 0.88 dL / g, 0.9 dL / g, and ranges formed by any two of the aforementioned values), further preferably 0.6-0.8 dL / g, and a standard deviation of the intrinsic viscosity of <0.015 dL / g.

[0037] According to the present application, preferably, the copolyethylene terephthalate has a melting point of 240°C or lower (e.g., 240°C, 230°C, 235°C, 220°C, 200°C, 190°C, 180°C, 170°C, 160°C, 150°C, 145°C, 140°C, 135°C, 130°C, 120°C, and ranges formed by any two of the aforementioned values), more preferably 130-240°C.

[0038] According to the present application, preferably, the copolyethylene terephthalate includes structural units from terephthalic acid, ethylene glycol and a comonomer Y, the content of structural units from the comonomer Y in the copolyethylene terephthalate being 0.6-18 mol% (for example, 0.6 mol%, 0.75 mol%, 1 mol%, 1.5 mol%, 3 mol%, 5 mol%, 10 mol%, 15 mol%, 18 mol%, 20 mol%, and a range consisting of any two of the above). In the present application, the content of structural units from the comonomer Y refers to the percentage of the amount of substance of structural units from the comonomer Y to the total amount of substance of terephthalic acid, ethylene glycol and structural units from the comonomer Y.

[0039] According to the present application, the kind of the comonomer is not particularly limited, as long as it can make the difference between the melting point and the intrinsic viscosity of the sheath material and the core material and the standard deviation of the intrinsic viscosity meet the requirements, preferably, the comonomer Y is selected from at least one of dicarboxylic acids, diols and tetraols. More preferably, the dicarboxylic acid is a dicarboxylic acid containing a benzene ring, and further preferably is isophthalic acid and / or phthalic acid. More preferably, the diol is a diol with a carbon atom number of 3-20, and further preferably is at least one of butanediol, hexanediol, cyclohexane dimethanol and pentanediol (for example, neopentane diol). More preferably, the tetraol is a tetraol with a carbon atom number of 3-20. In the present application, the carbon atom number of the diol or the tetraol can be independently 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 20.

[0040] In the present application, the comonomer Y can also be selected from acid monomers in other forms such as anhydride, acyl chloride, methyl ester, ethyl ester, etc., and alcohol monomers including alcohol monomers in other forms such as ether, hemiacetal, acetal, etc.

[0041] According to the present application, preferably, the mass ratio of the core material to the sheath material is x, 1.5≤x≤4.

[0042] In the present application, the amount of the core material is higher than that of the sheath material, and the mass ratio (weight ratio) of the core material to the sheath material can be 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.2, 3.5, 3.8, 4, and a range consisting of any two of the above, and preferably 2-3.6. Limiting the weight ratio of the core material to the sheath material within the above preferred range can further improve the uniformity of the thickness of the polyester fiber, i.e., obtain a smaller standard deviation of the diameter of the polyester fiber.

[0043] According to the present application, preferably, the mass ratio x of the core layer material to the skin layer material and the average diameter D of the polyester fiber satisfy the relational expression of formula (1) :

[0044]

[0045] According to the present application, the inventors have further found that when the fiber cloth prepared from the polyester fiber with the mass ratio x of the core layer material to the skin layer material and the average diameter D of the polyester fiber satisfying the relational expression of formula (1) is used for cell culture, the sugar metabolic capacity of the cells can be improved.

[0046] Further preferably, when the mass ratio of the core layer material to the skin layer material of the composite polyester fiber is 2-3 and formula (1) is satisfied, the sugar metabolic capacity of the cells when the composite polyester fiber is used for cell culture can be further improved.

[0047] According to the present application, preferably, the side blowing air has a speed of 1.5-1.8 m / s (for example, 1.5 m / s, 1.6 m / s, 1.7 m / s, 1.8 m / s, and a range formed by any two of the above) and a temperature of 15-20℃.

[0048] According to the present application, preferably, the slit has a pitch of 0.3-3 cm and a width of 10-60 cm. It can be understood that the width of the slit refers to the distance of the slit parallel to the long side direction of the spinneret. The length of the slit can be 10-60 cm. The length of the slit refers to the distance along the fiber ejection direction.

[0049] According to the present application, preferably, the nascent fiber is stretched in the slit by high-speed airflow, wherein the flow rate of the high-speed airflow is 2500-5500 m / min.

[0050] According to the present application, preferably, the temperature of the hot pressing is 180-220℃.

[0051] According to the present application, preferably, the content of Sb element in the core layer material and the skin layer material of the polyester fiber is less than 20 ppm. In the present application, "ppm" refers to the "weight" content.

[0052] The second aspect of the present application provides a polyester fiber cloth prepared by the above-mentioned method.

[0053] The third aspect of the present application provides a polyester fiber cloth, which is self-adhesive from polyester fibers with a skin-core structure, the thickness of the polyester fiber cloth is 0.4-0.5 mm, and the areal density is 80-130 g / m 2 ; wherein the average diameter of the polyester fiber is 17-32 μm, and the diameter standard deviation is ≤2.2 μm.

[0054] In the present application, the core material and the skin material of the polyester fiber are as described above, and will not be repeated here.

[0055] The fourth aspect of the present application provides the polyester fiber cloth as described above for use in cell culture.

[0056] According to the present application, preferably, the use includes improving the rate of cell glycometabolism. Preferably, the cell culture using the fiber cloth of the present application can make the rate of cell glycometabolism above 15 g / day, for example, 15-30 g / day.

[0057] The present application will be described in detail below by way of examples.

[0058] Without special instructions, the manufacturer of the spinneret is Changzhou Jier Precision Machinery Manufacturing Co., Ltd., and it is customized according to requirements.

[0059] The content of Sb element in the core material and the skin material is less than 20 ppm.

[0060] The room temperature is about "15-20℃".

[0061] In the following examples, the self-made polyester is prepared by mixing PTA (terephthalic acid, analytical pure), EG (ethylene glycol, analytical pure) and optional comonomer Y (such as cyclohexane dimethanol, analytical pure) in a reaction kettle according to the required amount of product, heating to 258-263℃, reacting for about 1 hour, then adding tetrabutyl titanate, increasing the temperature to 275-290℃, and vacuumizing with an oil pump, and reacting for about 2 hours to obtain the required PET.

[0062] Method for testing the average diameter of the polyester fiber: randomly take 50 polyester fibers, respectively randomly cut a small section, take scanning electron microscope (SEM) photos, and measure the diameter of each fiber from the photos, accurate to 0.1 μm, then calculate the average value of these diameter values according to the calculation method of average value; and calculate the standard deviation of these diameter values according to the calculation method of standard deviation.

[0063] Example 1

[0064] Homopolymerized polyethylene terephthalate (Sinopec Yizheng Chemical Fibre Co., Ltd., hereinafter referred to as "Yizheng Chemical Fibre", model FG600, intrinsic viscosity 0.675 dL / g, standard deviation of intrinsic viscosity 0.003 dL / g, melting point 261 ℃) was used as the core layer material. Copolymerized polyethylene terephthalate (Yizheng Chemical Fibre, model FG702, intrinsic viscosity 0.78 dL / g, standard deviation of intrinsic viscosity 0.006 dL / g, copolymerized monomer cyclohexane dimethanol, copolymerized monomer content 15 mol%, melting point 140 ℃) was used as the skin layer material. The mass ratio of the core layer material to the skin layer material was 3, and the polymer melt was extruded from the spinneret at an outlet temperature of about 280 ℃. The spinneret had 48 small holes with a skin-core concentric structure, the inner layer hole diameter was 20 microns, the range (the diameter of the largest hole minus the diameter of the smallest hole) was 1.2 microns, the outer layer hole diameter was 30 microns, and the range was 1.1 microns. After the polymer melt was extruded from the spinneret, it was blown and cooled by room temperature air at a speed of 1.5-1.8 m / s, and the cooled fiber was sent into a slit with a spacing of 1 cm, the width of the slit was 50 cm, and the length was 50 cm. The cooled fiber was stretched at a wind speed of 3500-4000 m / min to obtain polyester fiber. The average diameter of the polyester fiber was tested and the diameter standard deviation was calculated. The test results are shown in Table 1.

[0065] The obtained polyester fiber was laid by a laying machine, and then a certain thickness of the fiber cloth was laid. A non-embossed polished surface hot roller was used for hot pressing at 190-200 ℃ to obtain a fiber cloth with a specific thickness. The test method for the area density of the fiber cloth was as follows: a one-hundredth-of-a-gram balance was used to weigh the fiber cloth with an area of 1 cm 2 , and then the mass of the fiber cloth was divided by its area. The test method for the thickness of the fiber cloth was as follows: a dial gauge thickness gauge (model 547-313) from Suzhou Quantum Instrument Co., Ltd. was used. The area density and thickness of the fiber cloth are shown in Table 1.

[0066] Example 2

[0067] The method of Example 1 was followed, except that copolymerized polyethylene terephthalate (Yizheng Chemical Fibre, model BG804, intrinsic viscosity 0.801 dL / g, standard deviation of intrinsic viscosity 0.005 dL / g, copolymerized monomer cyclohexane dimethanol, copolymerized monomer content 0.75 mol%, melting point 237 ℃) was used as the skin layer material.

[0068] Example 3

[0069] The method of Example 1 was followed, except that the mass ratio of the core layer material to the skin layer material was 2, the inner layer hole diameter of the spinneret used was 18 microns, the range was 1.1 microns, the outer layer hole diameter was 32 microns, and the range was 1.6 microns.

[0070] Example 4

[0071] The method of Example 1 was followed, except that the mass ratio of the core material to the skin material was 3.6.

[0072] Example 5

[0073] The method of Example 1 was followed, except that the mass ratio of the core material to the skin material was 1.6.

[0074] Comparative Example 1

[0075] The method of Example 1 was followed, except that the mass ratio of the core material to the skin material was 1.

[0076] Comparative Example 2

[0077] The method of Example 1 was followed, except that the mass ratio of the core material to the skin material was 5.

[0078] Comparative Example 3

[0079] The method of Example 1 was followed, except that the raw material for the homopolyethylene terephthalate was a self-made polyester with an intrinsic viscosity of 0.51 dL / g, a standard deviation of intrinsic viscosity of 0.01 dL / g, and a melting point of 260°C.

[0080] Comparative Example 4

[0081] The method of Example 1 was followed, except that the small holes of the spinneret were not in a concentric circular structure, but rather a parallel structure with the circle divided into two. The inner diameter of the circular spinneret hole was 30 microns, and the range was 1.1 microns.

[0082] Comparative Example 5

[0083] The method of Example 1 was followed, except that the range of the hole diameter of the spinneret was large, the inner layer hole had an inner diameter of 20 microns and a range of 3.6 microns, and the outer layer hole had an inner diameter of 30 microns and a range of 5.3 microns.

[0084] Comparative Example 6

[0085] The method of Example 1 was followed, except that the raw material for the homopolyethylene terephthalate was BG85 from Yizheng Chemical Fibre, with an intrinsic viscosity of 0.879 dL / g, a standard deviation of intrinsic viscosity of 0.005 dL / g, and a melting point of 248°C.

[0086] Comparative Example 7

[0087] The method of Example 1 was followed, except that the raw material for the homopolyethylene terephthalate was a self-made polyester with an intrinsic viscosity of 0.675 dL / g, a standard deviation of intrinsic viscosity of 0.031 dL / g, and a melting point of 260°C; and the raw material for the copolyethylene terephthalate was a self-made polyester with an intrinsic viscosity of 0.782 dL / g, a standard deviation of intrinsic viscosity of 0.025 dL / g, and a melting point of 143°C, and the comonomer was cyclohexane dimethanol, and the comonomer content was 15 mol%.

[0088] Comparative Example 8

[0089] The method of Example 1 was followed, except that the raw material for the homopolyethylene terephthalate was BG804 from Yizheng Chemical Fibre Co., Ltd. with an intrinsic viscosity of 0.801 dL / g, a standard deviation of intrinsic viscosity of 0.005 dL / g, and a melting point of 237°C.

[0090] Comparative Example 9

[0091] The method of Example 1 was followed, except that the raw material for the copolyethylene terephthalate was BG85 from Yizheng Chemical Fibre Co., Ltd. with an intrinsic viscosity of 0.879 dL / g, a standard deviation of intrinsic viscosity of 0.005 dL / g, and a melting point of 248°C.

[0092] Comparative Example 10

[0093] The method of Example 1 was followed, except that the wind speed in the slit was 1500-2000 m / min.

[0094] Comparative Example 11

[0095] The method of Example 1 was followed, except that the melt filaments were not cooled in the slit, but were moderately stretched and laid down using a swing filament method.

[0096] Test Example 1

[0097] Pre-treatment and sterilization of the fiber cloth: the fiber cloth was soaked in a 7 wt% hydrogen peroxide aqueous solution at room temperature for 60 min, then was ultrasonically cleaned with ultrapure water, and then was dried at 80°C. The dried fiber cloth was subjected to high-temperature steam sterilization at 121°C for 60 min.

[0098] Then, the sugar metabolism ability of the vero cell using the fiber cloth after sterilization treatment as the carrier was tested. The testing method of the sugar metabolism was as follows: the fiber cloth was cut into small pieces of 6mmx6mm, 100g of the fiber cloth of 6mmx6mm, 8L of PBS buffer and about 200mL of 199 culture medium (purchased from Beijing Qingda Tianyi Biotechnology Co., Ltd.) were placed in a 10L basket reactor, 10% of superior new born calf serum (purchased from Lanzhou Minhai Biotechnology Co., Ltd.) was added, and an appropriate amount of 20% (by weight) glucose was added to make the total glucose concentration 0.5% (by weight). The vero cell (purchased from the National Biomedical Experimental Cell Resource Library, 4th generation vero cell, the inoculation amount of the cell on the fiber cloth of 1cm 2 was 0.5x10 6 ), and the culture was carried out at 37℃ under aeration, the pH was set to 7.3, the DO (dissolved oxygen accounted for the percentage of saturated dissolved oxygen) was 60%, the carbon dioxide concentration was 5%, and slow stirring was carried out, and the culture was carried out for 7 days. The glucose content was detected every 24h using a glucose detection kit (purchased from Nanjing Jiancheng Biological Engineering Institute), and the glucose concentration was supplemented to 0.5% (by weight) using a 20% (by weight) glucose solution. The measured decrease of the glucose content was converted into the glucose consumption rate value, the unit was g / day, the glucose consumption rate was plotted against the culture time, and then the value at 100h was taken as the 100h sugar metabolism rate value. The 100h sugar metabolism rate value is shown in Table 1.

[0099] Table 1

[0100]

[0101] The preferred embodiments of the present application are described in detail above, but the present application is not limited thereto. Within the technical concept of the present application, various simple modifications can be made to the technical solutions of the present application, including the combination of various technical features in any other suitable manner, and these simple modifications and combinations should also be considered as disclosed by the present application, and all fall within the protection scope of the present application.

Claims

1. A method for preparing polyester fiber fabric, characterized in that, The method includes: (1) The molten core material and the skin material are fed into a spinneret for spinning to obtain a filamentous melt with a core-skin structure; (2) Cooling the filamentous melt with a core-sheath structure to obtain nascent fibers; (3) The nascent fibers are stretched in a slit to obtain polyester fibers with a core-sheath structure; (4) Polyester fibers with an average diameter of 17-32 μm and a diameter standard deviation ≤ 2.2 μm are laid into a web and hot-pressed to obtain polyester fiber fabric; The mass ratio of the core material to the skin material is x, where 2 ≤ x ≤ 3; Among them, the mass ratio x of the core material to the skin material and the average diameter D of the polyester fiber satisfy the relationship of equation (1): Equation (1).

2. The method according to claim 1, wherein, The core layer material is homopolymer polyethylene terephthalate.

3. The method according to claim 2, wherein, The homopolymer polyethylene terephthalate has an intrinsic viscosity of 0.6-0.8 dL / g, a standard deviation of intrinsic viscosity ≤0.015 dL / g, and a melting point above 240℃.

4. The method according to claim 1, wherein, The skin material is copolymerized polyethylene terephthalate.

5. The method according to claim 4, wherein, The copolymerized polyethylene terephthalate has an intrinsic viscosity of 0.5-0.9 dL / g, a standard deviation of intrinsic viscosity ≤0.015 dL / g, and a melting point below 240℃.

6. The method according to claim 4, wherein, The copolymer ethylene terephthalate comprises structural units derived from terephthalic acid, ethylene glycol, and comonomer Y, wherein the content of structural units derived from comonomer Y in the copolymer ethylene terephthalate is 0.6-18 mol.

7. The method according to claim 6, wherein, The comonomer Y is selected from at least one of dicarboxylic acid, diol, and tetraol.

8. The method according to claim 1, wherein, The slits are spaced 0.3-3cm apart, 10-60cm wide, and 10-60cm long. And / or, the nascent fibers are stretched using a high-speed airflow in a slit, wherein the high-speed airflow velocity is 2500-5500 m / min.

9. The method according to claim 1, wherein, The hot pressing temperature is 180-220℃.

10. The polyester fiber fabric prepared by the method according to any one of claims 1-9.

11. A polyester fiber fabric prepared by the method according to any one of claims 1-9, characterized in that, This polyester fiber fabric is made of self-adhesive polyester fibers with a core-sheath structure. The thickness of the polyester fiber fabric is 0.4-0.5 mm, and the areal density is 80-130 g / m². 2 ; The average diameter of the polyester fiber is 17-32 μm, and the standard deviation of the diameter is ≤2.2 μm.

12. The use of the polyester fiber fabric according to claim 10 or 11 in cell culture.

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