Polyester fiber cloth, method for producing the same, and use thereof

Abstract

The application relates to the technical field of polyester fiber cloth, and discloses polyester fiber cloth, a preparation method and application thereof, the method comprising the following steps: obtaining primary fibers by cooling a filamentous melt with a skin-core structure; obtaining polyester fibers with a skin-core structure by stretching the primary fibers through a round tube method; and obtaining the polyester fiber cloth by laying and hot-pressing polyester fibers with an average diameter of 18-30 micrometers and a diameter standard deviation of less than or equal to 1.8 micrometers. When the fiber cloth prepared through the method is used for cell culture, the sugar metabolic capacity of the cells can be obviously improved, and the cell survival rate can be improved.

Description

Technical Field

[0001] This invention relates to the field of polyester fiber fabric technology, specifically to a polyester fiber fabric, its preparation method, and its application. Background Technology

[0002] Currently, large-scale animal cell culture technology is widely used in the production of various cells and cell products, including biopharmaceuticals such as enzymes, growth factors, vaccines, and antibodies. Most animal cells tend to adhere to surfaces, therefore, in vitro culture typically requires a support medium for their growth. This support medium is what those skilled in the art commonly refer to as a cell culture carrier. Common cell culture carriers in China include particulate carriers (2-11 mm in diameter), porous microsphere carriers (<1 mm in diameter), or sheet-like fiber cloth carriers. However, particulate carriers suffer from limitations such as cells only being able to adhere to the surface and having a small area-to-volume ratio. While porous microsphere carriers have pores that facilitate cell adhesion, these pores are easily blocked, affecting oxygen and nutrient exchange. Sheet-like fiber cloth carriers overcome these limitations and offer high culture efficiency, but related research is relatively limited both domestically and internationally. Current research mainly employs electrospinning techniques, primarily for in vivo tissue engineering transplantation, and rarely for large-scale in vitro cell culture due to its generally unsatisfactory overall culture results. Summary of the Invention

[0003] The purpose of this invention is to overcome the aforementioned problems in the prior art and to provide a polyester fiber fabric, its preparation method, and its application.

[0004] To achieve the above objectives, the first aspect of the present invention provides a method for preparing polyester fiber cloth, the method comprising: cooling a filamentous melt having a core-sheath structure to obtain nascent fibers, stretching the nascent fibers using a circular tube method to obtain polyester fibers having a core-sheath structure, and then taking polyester fibers with an average diameter of 18-30 μm and a diameter standard deviation ≤1.8 μm for web laying and hot pressing to obtain polyester fiber cloth.

[0005] A second aspect of the present invention provides a polyester fiber fabric prepared by the method described above.

[0006] A third aspect of the present invention provides a polyester fiber fabric, which is self-adhesively formed from polyester fibers having a core-sheath structure. The polyester fiber fabric has a thickness of 0.4-0.5 mm and an areal density of 80-130 g / m³. 2 ;

[0007] The average diameter of the polyester fiber is 18-30 μm, and the standard deviation of the diameter is ≤1.8 μm.

[0008] The fourth aspect of this invention provides the application of the aforementioned polyester fiber cloth in cell culture.

[0009] When the fiber cloth prepared by the method of the present invention is used for cell culture, it can significantly improve the sugar metabolism capacity of cells. The higher the sugar metabolism capacity, the higher the cell proliferation rate and survival rate (only living cells can metabolize sugar, and dead cells cannot metabolize sugar). That is, composite polyester fibers can achieve better results when used for cell culture. Detailed Implementation

[0010] The endpoints and any values ​​of the ranges disclosed herein are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of the various ranges, the endpoint values ​​of the various ranges and individual point values, and individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed herein.

[0011] The first aspect of the present invention provides a method for preparing polyester fiber cloth, the method comprising: a method for preparing polyester fiber cloth, characterized in that the method comprises: cooling a filamentous melt having a core-sheath structure to obtain nascent fibers, stretching the nascent fibers using a circular tube method to obtain polyester fibers having a core-sheath structure, and then taking polyester fibers with an average diameter of 18-30 μm and a diameter standard deviation ≤1.8 μm for web laying and hot pressing to obtain polyester fiber cloth.

[0012] According to the present invention, preferably, the melting point of the core material of the polyester fiber is more than 20°C higher than the melting point of the sheath material; the mass ratio of the core material to the sheath material is x, where 1.5 ≤ x ≤ 4.

[0013] According to the present invention, the melting point of the core layer material is more than 20°C higher than the melting point of the skin layer material (e.g., 20°C, 22°C, 24°C, 25°C, 30°C, 40°C, 50°C, 60°C, 80°C, 100°C, 110°C, 120°C, 130°C, 140°C, 150°C, 160°C, and any range of any two of the above points, such as 20-160°C). Preferably, the melting point of the core layer material differs from the melting point of the skin layer material by more than 24°C.

[0014] According to the present invention, the inventors have further discovered that when the fiber cloth prepared by using polyester fiber with the mass ratio x of core material to skin material and the average diameter D of polyester fiber satisfying the following formula (1) is used for cell culture, it can improve the sugar metabolism capacity of cells.

[0015]

[0016] More preferably, when the mass ratio of the core material to the sheath material of the composite polyester fiber is 2-3 and satisfies the formula (1), the sugar metabolism capacity of the cells can be further improved when the composite polyester fiber is used to culture cells.

[0017] As is well known in the art, the circular tube method in this invention refers to a method in which several (e.g., 2-20) nascent fibers are fed into a long circular tube, and the nascent fibers are stretched by air with a certain flow rate in the circular tube.

[0018] According to the present invention, preferably, the inner diameter of the circular tube used in the circular tube method is 0.5-5 cm, and the distance between two adjacent circular tubes is 0.5-10 cm. The distance between two adjacent circular tubes refers to the distance between the centers of the two adjacent circular tubes. The length of the circular tube can be 0.1-2 m. The length of the circular tube refers to the distance along the fiber ejection direction.

[0019] In this invention, the average diameter of the polyester fiber can be 18μm, 20μm, 22μm, 24μm, 26μm, 28μm, 30μm, or any range consisting of any two of the above points. The standard deviation of the polyester fiber diameter can be 1.8μm, 1.6μm, 1.4μm, 1.2μm, 1μm, 0.8μm, 0.6μm, 0.4μm, 0.2μm, 0.1μm, or any range consisting of any two of the above points, for example, 0.1-1.8μm.

[0020] In this invention, the method for testing the standard deviation of diameter is as follows: Take 50 polyester fibers at random, cut a small section from each fiber, take a scanning electron microscope (SEM) image, and measure the diameter of each fiber from the image to an accuracy of 0.1 μm. Then, calculate the standard deviation of these diameter values ​​according to the standard deviation calculation method.

[0021] In this invention, polyester fiber refers to polyester fiber with a length greater than 150 mm.

[0022] According to the present invention, preferably, the Sb content in the core and sheath materials of the polyester fiber is less than 20 ppm. In this invention, "ppm" refers to the content by weight.

[0023] According to the present invention, preferably, the intrinsic viscosity of the skin layer material is 0.01-0.18 dL / g higher than that of the core layer material, and the standard deviation of the intrinsic viscosity of the core layer material and the skin layer material is ≤0.015 dL / g.

[0024] In this invention, the intrinsic viscosity of polyester is tested using the intrinsic viscosity test method described in GB / T 14190-2017. Specifically, the standard deviation of the intrinsic viscosity is tested as follows: six samples are randomly selected, and their intrinsic viscosity is tested according to the intrinsic viscosity test method described in GB / T 14190-2017. The standard deviation of the values ​​is then recorded. A smaller standard deviation of the intrinsic viscosity indicates higher uniformity of the intrinsic viscosity within the same batch of material.

[0025] According to the present invention, the standard deviation of the intrinsic viscosity of the core material and the skin material can each be independently 0.018 dL / g, 0.015 dL / g, 0.01 dL / g, 0.009 dL / g, 0.008 dL / g, 0.007 dL / g, 0.006 dL / g, 0.005 dL / g, 0.004 dL / g, 0.003 dL / g, 0.002 dL / g, 0.001 dL / g, 0.0001 dL / g, and a range formed by any two of the above points, for example, 0.0001-0.018 dL / g.

[0026] According to the present invention, preferably, the core material of the polyester fiber is homopolymer polyethylene terephthalate. More preferably, the intrinsic viscosity of the homopolymer polyethylene terephthalate is 0.62-0.8 dL / g (e.g., 0.62 dL / g, 0.64 dL / g, 0.66 dL / g, 0.68 dL / g, 0.70 dL / g, 0.72 dL / g, 0.74 dL / g, 0.76 dL / g, 0.78 dL / g, 0.8 dL / g, and any two of the above ranges), and even more preferably 0.65-0.7 dL / g, with a standard deviation of intrinsic viscosity ≤0.01 dL / g.

[0027] According to the present invention, preferably, the homopolymer polyethylene terephthalate has a melting point of 240°C or higher (e.g., 240°C, 245°C, 248°C, 250°C, 255°C, 260°C, 265°C, 270°C, 280°C, 290°C, 300°C, 320°C, 350°C, 380°C, and any two of the above), more preferably 245-265°C.

[0028] According to the present invention, preferably, the sheath material of the polyester fiber is copolymerized polyethylene terephthalate. More preferably, the intrinsic viscosity of the copolymerized polyethylene terephthalate is 0.52-0.9 dL / g (e.g., 0.5 dL / g, 0.52 dL / g, 0.54 dL / g, 0.60 dL / g, 0.62 dL / g, 0.64 dL / g, 0.66 dL / g, 0.68 dL / g, 0.70 dL / g, 0.72 dL / g, 0.74 dL / g, 0.76 dL / g, 0.78 dL / g, 0.80 dL / g, 0.82 dL / g, 0.84 dL / g, 0.86 dL / g, 0.88 dL / g, 0.9 dL / g, and any two of the above ranges), and even more preferably 0.6-0.81 dL / g, with a standard deviation of intrinsic viscosity ≤0.01 dL / g.

[0029] According to the present invention, preferably, the melting point of the copolymerized polyethylene terephthalate is below 240°C (e.g., 240°C, 230°C, 235°C, 220°C, 200°C, 190°C, 180°C, 170°C, 160°C, 150°C, 145°C, 140°C, 135°C, 130°C, 120°C, and any two of the above), more preferably 130-240°C.

[0030] According to the present invention, preferably, the copolymerized ethylene terephthalate comprises structural units derived from terephthalic acid, ethylene glycol, and comonomer Y, wherein the content of structural units derived from comonomer Y in the copolymerized ethylene terephthalate is 0.6-20 mol% (e.g., 0.6 mol%, 0.75 mol%, 1 mol%, 1.5 mol%, 3 mol%, 5 mol%, 10 mol%, 15 mol%, 18 mol%, 20 mol%, and any combination thereof), more preferably 0.7-18 mol%. In the present invention, the content of structural units of comonomer Y refers to the percentage of the amount of substance of structural units of comonomer Y relative to the total amount of substance of structural units of terephthalic acid, ethylene glycol, and comonomer Y.

[0031] According to the present invention, the type of comonomer is not particularly limited, as long as the difference in melting point and intrinsic viscosity between the skin material and the core material, as well as the standard deviation of the intrinsic viscosity, meet the requirements. Preferably, the comonomer Y is selected from at least one of dicarboxylic acids, diols, and tetraols. More preferably, the dicarboxylic acid is a dicarboxylic acid containing a benzene ring, and further preferably isophthalic acid and / or phthalic acid. More preferably, the diol is a diol with 3-20 carbon atoms, and further preferably at least one of butanediol, hexanediol, cyclohexanediol, and pentanediol (e.g., neopentanediol). More preferably, the tetraol is a tetraol with 3-20 carbon atoms. In the present invention, the number of carbon atoms of the diol or tetraol can be independently 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, or 20.

[0032] In this invention, the comonomer Y may also be selected from acid monomers in other forms such as acid anhydrides, acyl chlorides, methanol esters, and ethanol esters, and alcohol monomers include alcohol monomers in other forms such as ethers, hemiacetals, and acetals.

[0033] According to the present invention, preferably, the temperature of the hot pressing is 180-220°C.

[0034] The present invention also provides a method for preparing polyester fibers, the method comprising: melting a core material and a sheath material separately to obtain a melt, and then spinning the melt to obtain polyester fibers having a core-sheath structure;

[0035] Among them, the melting point of the core material is more than 20°C higher than that of the skin material; the intrinsic viscosity of the skin material is 0.01-0.18 dL / g higher than that of the core material, and the standard deviation of the intrinsic viscosity of the core material and the skin material is ≤0.015 dL / g; the mass ratio of the core material to the skin material is x, and 1.5≤x≤4;

[0036] In the spinning process, the spinneret orifices are concentric circles. The difference between the maximum and minimum diameters of the inner circles of each spinneret orifice is ≤2μm, and the difference between the maximum and minimum diameters of the outer circles is ≤2μm. The difference between the maximum and minimum diameters of each inner circle or each outer circle can be independently 0.1μm, 0.5μm, 1.5μm, 2μm, or any range formed by any two of the above points, such as 0.1-2μm.

[0037] In this invention, the amount of core material is higher than that of sheath material. The mass ratio (weight ratio) of core material to sheath material can be 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.2, 3.5, 3.8, 4, or any two of the above, preferably 2-3.6. Limiting the weight ratio of core material to sheath material within the above preferred range can further improve the uniformity of polyester fiber thickness, i.e., obtain a smaller standard deviation of polyester fiber diameter.

[0038] In this invention, it is understood that the core material forms the core structure of polyester fiber after passing through the inner circle of the spinneret, and the sheath material forms the sheath structure of polyester fiber after passing through the outer circle of the spinneret. Then, after cooling and stretching, polyester fiber with a core-sheath structure is formed.

[0039] According to the present invention, preferably, the inner diameter of the inner circle of the spinneret orifice is 15-25 μm, and the inner diameter of the outer circle is 25-35 μm. In the present invention, the precise orifice diameter of the spinneret is measured by a profilometer.

[0040] According to the present invention, preferably, the core layer material melt is obtained by melting the core layer material at 220-280°C.

[0041] According to the present invention, preferably, the method of obtaining the skin material melt is to melt the skin material at 200-280°C.

[0042] In this invention, it can be understood that the temperature of the core material melt and the skin material melt refers to the temperature of the spinneret when they are extruded from the spinneret outlet.

[0043] According to the present invention, preferably, the process of spinning the melt to obtain polyester fibers with a core-sheath structure includes the following steps:

[0044] (1) The melt is fed into a spinneret for spinning to obtain a filamentous melt with a core-sheath structure;

[0045] (2) Primary fibers are obtained by cooling a filamentous melt with a core-sheath structure using side-blowing air.

[0046] (3) Polyester fibers are obtained by stretching the nascent fibers in a circular tube using a high-speed airflow.

[0047] According to the present invention, preferably, the wind speed of the side-blowing air is 1.5-1.8 m / s (e.g., 1.5 m / s, 1.6 m / s, 1.7 m / s, 1.8 m / s, and any two of the above ranges), and the temperature is 15-20°C.

[0048] According to the present invention, preferably, the flow rate of the high-speed airflow is 3000-5000 m / min. Preferably, high-speed airflow stretching allows the fiber structure to be further preferentially oriented along the fiber processing direction, thereby making the fiber thickness more uniform and improving its mechanical properties.

[0049] A second aspect of the present invention provides a polyester fiber fabric prepared by the method described above.

[0050] A third aspect of the present invention provides a polyester fiber fabric, which is self-adhesively formed from polyester fibers having a core-sheath structure. The polyester fiber fabric has a thickness of 0.4-0.5 mm and an areal density of 80-130 g / m³. 2 ;

[0051] The average diameter of the polyester fiber is 18-30 μm, and the standard deviation of the diameter is ≤1.8 μm.

[0052] In this invention, the core material and sheath material in the polyester fiber are as described above and will not be repeated here.

[0053] The fourth aspect of this invention provides the application of the aforementioned polyester fiber cloth in cell culture.

[0054] According to the present invention, preferably, the application includes increasing the rate of cellular glucose metabolism. Preferably, cell culture using the fiber cloth of the present invention can achieve a cellular glucose metabolism rate of 15 g / day or higher, for example, 15-30 g / day.

[0055] The present invention will be described in detail below through embodiments.

[0056] The present invention will be described in detail below through embodiments. In the following embodiments,

[0057] Unless otherwise specified, the spinneret is manufactured by Changzhou Jier Precision Machinery Manufacturing Co., Ltd., and is customized according to requirements.

[0058] The Sb content in both the core material and the skin material is less than 20 ppm.

[0059] The room temperature is approximately 15-20℃.

[0060] In the following examples, the self-made polyester involved is prepared by mixing PTA (terephthalic acid, analytical grade), EG (ethylene glycol, analytical grade) and optional comonomer Y (e.g., cyclohexanediol, analytical grade) in a reaction vessel according to the required amount of product, heating to 258-263°C, reacting for about 1 hour, then adding tetrabutyl titanate, raising the temperature to 275-290°C, and using an oil pump to evacuate the vacuum, reacting for about 2 hours to obtain the desired PET.

[0061] Test method for average diameter of polyester fiber: Take 50 polyester fibers, randomly cut a small section from each fiber, take a scanning electron microscope (SEM) image, and measure the diameter of each fiber from the image to an accuracy of 0.1 μm. Then calculate the average value of these diameter values ​​according to the method for calculating the average value; and calculate the standard deviation of these diameter values ​​according to the method for calculating the standard deviation.

[0062] Example 1

[0063] Homopolymer polyethylene terephthalate (manufactured by Yizheng Chemical Fiber Co., Ltd. of Sinopec, hereinafter referred to as "Yizheng Chemical Fiber", model FG600, intrinsic viscosity 0.675 dL / g, intrinsic viscosity standard deviation 0.003 dL / g, melting point 261℃) was used as the core layer material. Copolymer polyethylene terephthalate (Yizheng Chemical Fiber, model FG702, intrinsic viscosity 0.78 dL / g, intrinsic viscosity standard deviation 0.006 dL / g, comonomer is cyclohexanediol, comonomer content is 15 mol%, melting point 140℃) was used as the skin layer material. The mass ratio of core layer material to skin layer material was 3, and the material was extruded from the spinneret at an outlet temperature of approximately 280℃. The spinneret has 48 concentric core-shell holes. The inner hole diameter is 20 micrometers with a range (the difference between the largest and smallest holes) of 1.2 micrometers, while the outer hole diameter is 30 micrometers with a range of 1.1 micrometers. After the polymer melt is extruded from the spinneret, it is cooled by room temperature air at 1.5-1.8 m / s. The cooled fibers are then fed evenly or nearly evenly into five steel tubes (each tube has an inner diameter of 2 cm, a distance of 3.5 cm between adjacent tubes, and a length of 1 m). The cooled fibers are stretched within the tubes at an air velocity of approximately 4000-4500 m / min to obtain polyester fibers. The average diameter and calculated standard deviation of the polyester fibers were measured. The test results are shown in Table 1.

[0064] The obtained polyester fibers are laid into a web using a web-laying machine. Then, the fiber web of a certain thickness is hot-pressed at 190-200℃ using a polished, non-embossed hot roller to obtain a fiber fabric of a specific thickness. The areal density of the fiber fabric is tested using a 0.01% balance to measure the density of 1 cm². 2 The area of ​​the fiber cloth was weighed, and then the mass of the fiber cloth was divided by its area. The thickness of the fiber cloth was tested using a dial-type thickness gauge (model 547-313) from Suzhou Quantum Instruments Co., Ltd. The areal density and thickness of the fiber cloth are shown in Table 1.

[0065] Example 2

[0066] The method of Example 1 was followed, except that a copolymer of polyethylene terephthalate (Yizheng Chemical Fiber, model BG804, intrinsic viscosity 0.801 dL / g, intrinsic viscosity standard deviation 0.005 dL / g, comonomer is cyclohexanediol, comonomer content is 0.75 mol%, melting point is 237℃) was used as the skin material.

[0067] Example 3

[0068] The method was carried out according to Example 1, except that the mass ratio of the core material to the skin material was 2, the inner diameter of the spinneret was 18 micrometers with a range of 1.1 micrometers, and the outer diameter was 32 micrometers with a range of 1.6 micrometers.

[0069] Example 4

[0070] The method was carried out according to Example 1, except that the mass ratio of the core material to the skin material was 3.6.

[0071] Example 5

[0072] The method was carried out according to Example 1, except that the mass ratio of the core material to the skin material was 1.6.

[0073] Comparative Example 1

[0074] The method was carried out according to Example 1, except that the mass ratio of the core material to the skin material was 1.

[0075] Comparative Example 2

[0076] The method was carried out according to Example 1, except that the mass ratio of the core material to the skin material was 5.

[0077] Comparative Example 3

[0078] The method was carried out according to Example 1, except that the raw material used for the homopolymer polyethylene terephthalate was a self-made polyester with an intrinsic viscosity of 0.51 dL / g, a standard deviation of intrinsic viscosity of 0.01 dL / g, and a melting point of 260°C.

[0079] Comparative Example 4

[0080] The method was carried out according to Example 1, except that the spinneret orifices were not concentric circles, but rather a parallel structure that divided the circle in two. The inner diameter of the circular spinneret orifice was 30 micrometers, and the range was 1.1 micrometers.

[0081] Comparative Example 5

[0082] The method was carried out according to Example 1, except that the spinneret had a larger aperture range, with the inner layer aperture being 20 micrometers and the range being 3.6 micrometers, and the outer layer aperture being 30 micrometers and the range being 5.3 micrometers.

[0083] Comparative Example 6

[0084] The method was carried out according to Example 1, except that the raw material used for homopolymer polyethylene terephthalate was Yizheng Chemical Fiber's BG85, with an intrinsic viscosity of 0.879 dL / g, a standard deviation of intrinsic viscosity of 0.005 dL / g, and a melting point of 248°C.

[0085] Comparative Example 7

[0086] The method was carried out according to Example 1, except that the raw material used for homopolymer polyethylene terephthalate was a self-made polyester with an intrinsic viscosity of 0.675 dL / g, a standard deviation of intrinsic viscosity of 0.031 dL / g, and a melting point of 260°C; the raw material used for copolymer polyethylene terephthalate was a self-made polyester with an intrinsic viscosity of 0.782 dL / g, a standard deviation of intrinsic viscosity of 0.025 dL / g, and a melting point of 143°C. The comonomer was cyclohexanediol with a comonomer content of 15 mol%.

[0087] Comparative Example 8

[0088] The method was carried out according to Example 1, except that the raw material used for homopolymer polyethylene terephthalate was Yizheng Chemical Fiber's BG804, with an intrinsic viscosity of 0.801 dL / g, a standard deviation of intrinsic viscosity of 0.005 dL / g, and a melting point of 237°C.

[0089] Comparative Example 9

[0090] The method was carried out in accordance with Example 1, except that the raw material used for the copolymerized polyethylene terephthalate was Yizheng Chemical Fiber's BG85, with an intrinsic viscosity of 0.879 dL / g, a standard deviation of intrinsic viscosity of 0.005 dL / g, and a melting point of 248°C.

[0091] Comparative Example 10

[0092] The method was carried out according to Example 1, except that the wind speed in the circular pipe was 1500-2000 m / min.

[0093] Comparative Example 11

[0094] The method is carried out according to Example 1, except that the melt wire does not enter the round tube after cooling, but is stretched and laid out using the oscillating wire method.

[0095] Test Example 1

[0096] Pretreatment and sterilization of the fiber cloth: The fiber cloth was soaked in a 7% (w / w) hydrogen peroxide aqueous solution at room temperature for 60 minutes; then, it was ultrasonically cleaned with ultrapure water and dried at 80°C. The dried fiber cloth was then sterilized by high-temperature steam at 121°C for 60 minutes.

[0097] Then, the glucose metabolism capacity of Vero cells was tested using sterilized fiber cloth as a carrier. The glucose metabolism test method was as follows: Fiber cloth was cut into 6mm × 6mm pieces. 100g of the 6mm × 6mm fiber cloth, 8L of PBS buffer, and approximately 200mL of 199 culture medium (purchased from Beijing Tsinghua Tianyi Biotechnology Co., Ltd.) were placed in a 10L basket reactor. 10% of superior newborn calf serum (purchased from Lanzhou Minhai Biotechnology Co., Ltd.) relative to the culture medium was added, along with an appropriate amount of 20% by weight glucose to bring the total glucose concentration to 0.5% by weight. Vero cells (purchased from the National Biomedical Experimental Cell Resource Bank, fourth-generation Vero cells, per 1cm²) were then seeded. 2 The inoculation density of cells on the fiber cloth was 0.5 × 10⁻⁶. 6 Individual samples were cultured at 37℃ with aeration. During cultivation, the pH was set to 7.3, DO (dissolved oxygen as a percentage of saturated dissolved oxygen) to 60%, and the carbon dioxide concentration to 5%, with slow stirring for 7 days. Samples were taken every 24 hours, and glucose content was measured using a glucose assay kit (purchased from Nanjing Jiancheng Bioengineering Institute). The glucose concentration was then adjusted to 0.5% by weight using 20% ​​glucose solution. The decrease in glucose content was converted to a glucose consumption rate value, expressed in g / day. A curve was plotted on the glucose consumption rate against cultivation time, and the value at 100 hours was taken as the 100-hour glucose metabolism rate value. The 100-hour glucose metabolism rate values ​​are shown in Table 1.

[0098] Table 1

[0099]

[0100] The preferred embodiments of the present invention have been described in detail above; however, the present invention is not limited thereto. Within the scope of the inventive concept, various simple modifications can be made to the technical solutions of the present invention, including combinations of various technical features in any other suitable manner. These simple modifications and combinations should also be considered as the content disclosed in the present invention and are all within the protection scope of the present invention.

Claims

1. A method of producing a polyester fiber cloth, characterized by, The method comprises: cooling a filamentous melt having a sheath-core structure to obtain a primary fiber, stretching the primary fiber by a round tube method to obtain a polyester fiber having a sheath-core structure, and then laying and hot-pressing the polyester fiber having an average diameter of 18-30 μm and a diameter standard deviation of ≤1.8 μm to obtain a polyester fiber cloth. The mass ratio of the core layer material to the sheath layer material is x, and 2≤x≤3. The mass ratio x of the core layer material to the sheath layer material in the polyester fiber and the average diameter D of the polyester fiber satisfy the relationship of formula (1). Formula (1).

2. The method of claim 1, wherein, The melting point of the core layer material of the polyester fiber is higher than that of the sheath layer material by 20°C or more.

3. The method of claim 1, wherein, The core layer material of the polyester fiber is homopolymer polyethylene terephthalate.

4. The method of claim 3, wherein, The homopolymer polyethylene terephthalate has an intrinsic viscosity of 0.62-0.8 dL / g, an intrinsic viscosity standard deviation of ≤0.01 dL / g, and a melting point of 245-265°C.

5. The method of claim 1, wherein, The sheath layer material of the polyester fiber is copolymer polyethylene terephthalate.

6. The method of claim 5, wherein, The copolymer polyethylene terephthalate has an intrinsic viscosity of 0.52-0.9 dL / g, an intrinsic viscosity standard deviation of ≤0.01 dL / g, and a melting point of 130-240°C.

7. The method of claim 5, wherein, The copolymer polyethylene terephthalate comprises structural units from terephthalic acid, ethylene glycol, and a comonomer Y, and the content of the structural units from the comonomer Y in the copolymer polyethylene terephthalate is 0.6-20 mol%.

8. The method of claim 7, wherein, The content of the structural units from the comonomer Y in the copolymer polyethylene terephthalate is 0.7-18 mol%.

9. The method of claim 7, wherein, The comonomer Y is selected from at least one of a dicarboxylic acid, a dihydric alcohol, and a tetrahydric alcohol.

10. The method of claim 1, wherein, The temperature of the hot-pressing is 180-220°C.

11. The polyester fiber cloth prepared by the method of any one of claims 1-10.

12. A polyester fiber sheet prepared by the method according to any one of claims 1 to 10, characterized in that, The polyester fiber cloth is self-adhered by polyester fibers having a core-sheath structure, the thickness of the polyester fiber cloth is 0.4-0.5mm, and the areal density is 80-130g / m 2 ; The average diameter of the polyester fiber is 18-30 μm, and the diameter standard deviation is ≤1.8 μm.

13. Use of the polyester fiber cloth of claim 11 or 12 in cell culture.

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