Artificial breeding method of cynanchum otophyllum

Abstract

The artificial breeding method comprises the following steps: parent fish culture: selecting wild cymbidium for cage culture, and feeding culture bait; after the weight of the parent fishes reaches the standard that the weight of the female fishes is larger than 200 g / fish and the weight of the male fishes is larger than 150 g / fish, round fishes which are robust in physique, lively in action, high in stress resistance and free of body surface trauma are selected as the parent fishes, the parent fishes are transferred into the parent fish pond from the net cage, and the proportion of the female parent fishes to the male parent fishes is (2-2.5): 1. According to the method disclosed by the invention, the fortified bait prepared from the iced trash fish, the eel meal, the probiotic, the multivitamins, the fish oil, the vitality-promoting agent and the yeast is used for fortifying the parent fish of the Cymbidium, and a certain dosage of HCG is used for carrying out ripening and spawning induction treatment on the female parent and the male parent, so that the Cymbidium fry can be obtained on a large scale; therefore, the artificial breeding of the cynanchum otophyllum fries is realized, the blank of the artificial breeding technology of the cynanchum otophyllum is filled, a foundation is provided for the large-scale production of the cynanchum otophyllum fries, and the popularization of

Description

Technical Field

[0001] The invention relates to the technical field of blue tuna breeding, and in particular to an artificial breeding method for blue tuna. Background Art

[0002] The blue round trevally is a fish of the genus Trevally in the family Trevally. It has a slightly flattened body and is spindle-shaped. The blue round trevally is distributed in the northwest Pacific Ocean and surrounding countries.

[0003] The blue round trevally is a warm-water pelagic fish that often gathers in groups when the tide is slow, the weather is clear and there is southeast wind. During the day, they will float up in groups, making the sea surface appear grayish black; at night, they have a weak tendency to phototaxis. The main food includes zooplankton such as krill, copepods, amphipods, ostracods, and small fish. The fishing season in the East China Sea is from May to November. The spring flood is in spawning groups, and the individuals are larger; the autumn flood is in feeding groups, and the individuals are smaller. The suitable temperature for spawning is 15-22 degrees. The body length is composed of 140-180 mm and 220-270 mm. The fishing season in the South China Sea is from March to May, and the spawning group consists of individuals of 120-270 mm.

[0004] Blue tuna has the characteristics of fast growth, strong swimming ability and high-density farming, which makes it very suitable for deep-sea farming. However, there are no reports on artificial breeding of blue tuna. Since the artificial breeding technology of blue tuna has not yet been broken through, there are no fry for the time being, so large-scale farming is impossible. Summary of the invention

[0005] The invention provides an artificial breeding method for blue round tuna, which can effectively ripen and induce spawning of blue round tuna broodstock, realize artificial breeding of blue round tuna fry, and provide a basis for large-scale production of blue round tuna seedlings.

[0006] The technical solution of the present invention is achieved in this way: An artificial breeding method for blue round fish comprises: Parent fish cultivation: Select wild blue round fish for cage culture and feed them with culture feed; Broodstock transplantation: When the weight of broodstock reaches the standard of more than 200g / tail for female fish and more than 150g / tail for male fish, select roundtails with strong physique, active movements, strong resistance and no external injuries on the body surface as broodstock, and transfer the broodstock from the net cage to the broodstock pond. The ratio of female broodstock to male broodstock is 2-2.5:1; Broodstock cultivation: The broodstock in the broodstock pond are fed with enhanced feed for 15-20 days. After 7 days of intensive cultivation, the sexual maturity of the broodstock is checked. When the gonads of male broodstock can squeeze out semen, they are transferred to the breeding pond for standby use. At this time, female broodstock with enlarged abdomen, soft to touch and red genital opening are selected for artificial induced spawning, while broodstock that are not sexually mature continue to be intensively cultivated; Artificial induction of labor: After the abdomen of the broodstock is obviously swollen, calculate the fullness of the broodstock, fullness = body weight (g) / [body length (cm)]³ × 100%. When the fullness is greater than 3%, artificial induction of labor is performed by back muscle injection. The dosage of oxytocin for male fish is 1 / 3 of that for female fish. If the female fish does not lay eggs after one week, continue to inject, but the male fish does not need to be injected. Each broodstock can be injected up to 2 times; Natural fertilization and hatching: The artificially induced female and male broodstock are placed in a spawning pond. After the broodstock lay eggs, they are disinfected with a 1 mg / L sodium penicillin seawater solution for 1 hour and then placed in a larval breeding pond for hatching to obtain blue round fish fry.

[0007] Preferably, in the broodstock cultivation step, the aquaculture bait consists of shrimp, water, kelp powder, spirulina powder and yeast, and the weight ratio of the shrimp, water, kelp powder, spirulina powder and yeast is 100:70:2:1:1. A small amount of water is added to the shrimp compound feed particles to make them sticky, and then the kelp powder and spirulina powder are mixed evenly, and then air-dried to form particles to form the aquaculture bait.

[0008] Preferably, in the step of raising broodstock, the stocking density of cage culture is 6-10 fish / m3, the daily feeding amount of the culture feed is 1%-2% of the fish body weight, the feed is fed 2-3 times a day, and the culture period is 3-6 months.

[0009] Preferably, in the broodstock transplantation step, the bottom area of ​​the broodstock pond is 15-20m², the depth of the broodstock pond is 1.8-2.2m, 80-90 broodstock are placed in each pond, 15-18 air stones are placed on the bottom of the broodstock pond, shading nets are arranged at intervals 1.6-2m above the broodstock pond, 8-10 nylon meshes are placed on the bottom of the pond, the water is changed 1-3 times a day, the water change amount is 100%, the bottom is sucked every day, and the pond is emptied once every 5-7 days.

[0010] Preferably, in the broodstock transplantation step, the best time to catch the broodstock is early in the morning, no feeding is done on that day, the transport density is 60-75 fish / m³, the transport water temperature is 17°C-20°C, and the transport time is less than 6 hours.

[0011] Preferably, in the broodstock cultivation step, the fortified bait consists of chilled fish, eel meal, probiotics, multivitamins, fish oil, vitality and yeast, and the mass ratio of the chilled fish, eel meal, probiotics, multivitamins, fish oil, vitality and yeast is 400:800:1:1:1:1:1.

[0012] Preferably, in the artificial induction step, the amount of HCG used as the oxytocin for female broodstock is 450-550 IU / kg, diluted to 0.5-1 ml with physiological saline.

[0013] Preferably, in the steps of natural fertilization and hatching, the water temperature of the larvae rearing pool is 25°C-28°C and the hatching time is 24-48 hours.

[0014] Preferably, in the natural fertilization and hatching steps, the salinity of the water is 30-32‰, the pH value is between 7.5-7.8, and the dissolved oxygen in the water is 4.00-6.00 mg / L.

[0015] Beneficial effects: The invention uses a reinforced feed prepared by mixing fresh ice fish, eel meal, probiotics, multivitamins, fish oil, vitality and yeast to strengthen the cultivation of blue round tuna broodstock, and uses a certain dose of HCG to accelerate the ripening and induce the production of female and male parents, so that blue round tuna fry can be obtained on a large scale, thereby realizing artificial propagation of blue round tuna fry, filling the gap in artificial propagation technology of blue round tuna, providing a basis for large-scale production of blue round tuna seedlings, and being conducive to the promotion of blue round tuna. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0017] Figure 1 The present invention is a process flow chart of an artificial breeding method of blue round fish according to an embodiment of the present invention. DETAILED DESCRIPTION

[0018] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field belong to the scope of protection of the present invention.

[0019] According to an embodiment of the present invention, an artificial breeding method for blue trevally is provided.

[0020] like Figure 1 As shown, in this optional embodiment, the artificial breeding method of blue round fish according to the embodiment of the present invention includes: Step S101, broodstock cultivation: selecting wild blue round tuna for cage culture and feeding them with culture feed; Step S103, broodstock transplantation: when the weight of the broodstock reaches the standard of more than 200g / tail for female fish and more than 150g / tail for male fish, roundtails with strong physique, active movement, strong resistance and no external injuries on the body surface are selected as broodstock, and the broodstock are transferred from the net cage to the broodstock pond, with the ratio of female broodstock to male broodstock being 2-2.5:1; Step S105, broodstock culture: the broodstock in the broodstock pond are fed with fortified feed for fortified culture for 15-20 days. After 7 days of fortified culture, the sexual maturity of the broodstock is checked. When the gonads of the male broodstock can squeeze out semen, they are transferred to the breeding pond for standby use. At this time, female broodstock with enlarged abdomen, soft to touch and red genital pores are selected for artificial induced spawning, while broodstock that are not sexually mature continue to be fortified culture; Step S107, artificial induction of labor: after the abdomen of the broodstock is obviously swollen, calculate the fullness of the broodstock, fullness = body weight (g) / [body length (cm)]³ × 100%. When the fullness is greater than 3%, artificial induction of labor is performed by back muscle injection. The dosage of oxytocin for male fish is 1 / 3 of that for female fish. If the female fish does not lay eggs after one week, continue to inject, and the male fish does not need to be injected. Each broodstock can be injected twice at most; Step S109, natural fertilization and hatching: the female broodstock and the male broodstock after artificial induction of spawning are placed in a spawning pond. After the broodstock lay eggs, they are disinfected with a 1 mg / L sodium penicillin seawater solution for 1 hour, and then placed in a larval breeding pond for hatching to obtain blue round tuna fry. After the membrane is hatched, the blue round tuna fry are gently poured out into the larval breeding pond for hatching.

[0021] In this optional embodiment, in the broodstock cultivation step, the breeding bait is composed of shrimp, water, kelp powder, spirulina powder and yeast, and the weight ratio of the shrimp, water, kelp powder, spirulina powder and yeast is 100:70:2:1:1. A small amount of water is added to the shrimp compound feed particles to make them sticky, and then the kelp powder and spirulina powder are mixed evenly, and then air-dried to make them into particles to form the breeding bait.

[0022] In this optional embodiment, in the parent fish cultivation step, the stocking density of cage culture is 6-10 fish / m3, the daily feeding amount of the culture feed is 1%-2% of the fish body weight, the feed is fed 2-3 times a day, and the culture period is 3-6 months.

[0023] In this optional embodiment, in the broodstock transplantation step, the bottom area of ​​the broodstock pond is 15-20m², the depth of the broodstock pond is 1.8-2.2m, 80-90 broodstock are placed in each pond, 15-18 air stones are placed on the bottom of the broodstock pond, shading nets are arranged at intervals 1.6-2m above the broodstock pond, 8-10 nylon meshes are placed on the bottom of the pond, the water is changed 1-3 times a day, the water change volume is 100%, the bottom is sucked every day, and the pond is emptied once every 5-7 days.

[0024] In this optional embodiment, in the broodstock transplantation step, the best time to catch the broodstock is early morning, no feeding is done on that day, the transportation density is 60-75 fish / m³, the transportation water temperature is 17°C-20°C, and the transportation time is less than 6 hours.

[0025] In this optional embodiment, in the broodstock cultivation step, the fortified bait is composed of chilled miscellaneous fish, eel meal, probiotics, multivitamins, fish oil, vitality and yeast, and the mass ratio of the chilled miscellaneous fish, eel meal, probiotics, multivitamins, fish oil, vitality and yeast is 400:800:1:1:1:1:1.

[0026] In this optional embodiment, in the artificial induction step, the amount of oxytocin HCG used for the female broodstock is 450-550 IU / kg, which is diluted to 0.5-1 ml with physiological saline.

[0027] In this optional embodiment, in the steps of natural fertilization and hatching, the water temperature of the larvae rearing pond is 25°C-28°C and the hatching time is 24-48 hours.

[0028] In this optional embodiment, during the natural fertilization and hatching steps, the salinity of the water body is 30-32‰, the pH value is between 7.5-7.8, and the dissolved oxygen in the water body is 4.00-6.00 mg / L.

[0029] In order to facilitate understanding of the above technical solutions of the present invention, the above technical solutions of the present invention are further described below through a number of specific embodiments.

[0030] Embodiment 1 like Figure 1 As shown, in specific application, the artificial breeding method of blue round fish can be as follows: Parent fish cultivation: wild blue round trevally are selected for cage culture, and the cage culture is fed with culture feed. The culture density of cage culture is 6-10 fish / m3, and the daily feeding amount of culture feed is 1%-2% of the fish body weight, and the fish is fed 2-3 times a day. The culture period is 3 months; Broodstock transplantation: When the weight of broodstock reaches the standard of more than 200g / tail for female fish and more than 150g / tail for male fish, select roundtails with strong physique, active movement, strong resistance and no external injuries on the body surface as broodstock, and transfer the broodstock from the net cage to the broodstock pond. The ratio of female broodstock to male broodstock is 2:1. The bottom area of ​​the broodstock pond is 15m², the depth of the broodstock pond is 1.8m, and 80 broodstock are placed in each pond. 15 air stones are placed at the bottom of the broodstock pond. Shading nets are arranged at intervals of 1.6 above the broodstock pond. 8 nylon meshes are placed at the bottom of the pond. The water is changed once a day, and the water change volume is 100%. The bottom is sucked every day, and the pond is emptied once every 5 days. Broodstock cultivation: Broodstock in the broodstock pond are fed with enhanced feed for 15 days. After 7 days of intensive cultivation, the sexual maturity of the broodstock is checked. When the gonads of male broodstock can squeeze out semen, they are transferred to the breeding pond for standby use. At this time, female broodstock with enlarged abdomen, soft to touch and red genital opening are selected for artificial induced spawning, while broodstock that are not sexually mature continue to be intensively cultivated; Artificial induction of labor: After the abdomen of the broodstock is obviously swollen, calculate the fullness of the broodstock, fullness = body weight (g) / [body length (cm)]³ × 100%. When the fullness is greater than 3%, artificial induction of labor is performed, and the back muscle injection is performed. The dosage of oxytocin for male fish is 1 / 3 of that for female fish. If the female fish does not lay eggs after one week, continue to inject, and the male fish does not need to be injected. Each broodstock can be injected twice at most. The dosage of oxytocin HCG for female broodstock is 450IU / kg, diluted to 0.5ml with saline; Natural fertilization and hatching: The artificially induced female and male broodstock are placed in a spawning pond. After the broodstock lay eggs, they are disinfected with a 1 mg / L sodium penicillin seawater solution for 1 hour and then placed in a larval breeding pond for hatching to obtain blue round tuna fry. The water temperature of the larval breeding pond is 25°C and the hatching time is 24 hours. The salinity of the water in the larval breeding pond is 30‰, the pH value is between 7.5, and the dissolved oxygen in the water is 4.00 mg / L.

[0031] Embodiment 2 like Figure 1 As shown, in specific application, the artificial breeding method of blue round fish can be as follows: Parent fish cultivation: wild blue round trevally are selected for cage culture, and the cage culture is fed with culture feed. The culture density of cage culture is 6-10 fish / m3, and the daily feeding amount of culture feed is 1%-2% of the fish body weight, and the fish is fed 2-3 times a day. The culture period is 4.5 months; Broodstock transplantation: When the weight of broodstock reaches the standard of more than 200g / tail for female fish and more than 150g / tail for male fish, select roundtails with strong physique, active movement, strong resistance and no external injuries on the body surface as broodstock, and transfer the broodstock from the net cage to the broodstock pond. The ratio of female broodstock to male broodstock is 2.3:1. The bottom area of ​​the broodstock pond is 17m², the depth of the broodstock pond is 2m, and 82 broodstock are placed in each pond. 16 air stones are placed at the bottom of the broodstock pond. Shading nets are arranged at intervals of 1.8m above the broodstock pond. 9 nylon meshes are placed at the bottom of the pond. The water is changed twice a day, and the water change volume is 100%. The bottom is sucked every day, and the pond is emptied once every 6 days. Broodstock cultivation: Broodstock in the broodstock pond are fed with enhanced feed for 18 days. After 7 days of intensive cultivation, the sexual maturity of the broodstock is checked. When the gonads of male broodstock can squeeze out semen, they are transferred to the breeding pond for standby use. At this time, female broodstock with enlarged abdomen, soft to touch and red genital opening are selected for artificial induction of spawning, while broodstock that are not sexually mature continue to be intensively cultivated; Artificial induction of labor: After the abdomen of the broodstock is obviously swollen, calculate the fullness of the broodstock, fullness = body weight (g) / [body length (cm)]³ × 100%. When the fullness is greater than 3%, artificial induction of labor is performed, and the back muscle injection is performed. The dosage of oxytocin for male fish is 1 / 3 of that for female fish. If the female fish does not lay eggs after one week, continue to inject, and the male fish does not need to be injected. Each broodstock can be injected twice at most. The dosage of oxytocin HCG for female broodstock is 500IU / kg, diluted to 0.7ml with saline; Natural fertilization and hatching: The artificially induced female and male broodstock are placed in a spawning pond. After the broodstock lay eggs, they are disinfected with a 1 mg / L sodium penicillin seawater solution for 1 hour and then placed in a larval breeding pond for hatching to obtain blue round tuna fry. The water temperature of the larval breeding pond is 26.5°C and the hatching time is 36 hours. The salinity of the water in the larval breeding pond is 31‰, the pH value is between 7.65, and the dissolved oxygen in the water is 5.00 mg / L.

[0032] Embodiment 3 like Figure 1 As shown, in specific application, the artificial breeding method of blue round fish can be as follows: Parent fish cultivation: wild blue round tuna are selected for cage culture, and the cage culture is fed with culture feed. The culture density of cage culture is 6-10 fish / m3, and the daily feeding amount of culture feed is 1%-2% of the fish body weight, and the fish is fed 2-3 times a day. The culture period is 6 months; Broodstock transplantation: When the weight of broodstock reaches the standard of more than 200g / tail for female fish and more than 150g / tail for male fish, select roundtails with strong physique, active movement, strong resistance and no external injuries on the body surface as broodstock, and transfer the broodstock from the net cage to the broodstock pond. The ratio of female broodstock to male broodstock is 2.5:1. The bottom area of ​​the broodstock pond is 20m², the depth of the broodstock pond is 2.2m, and 90 broodstock are placed in each pond. 18 air stones are placed at the bottom of the broodstock pond. Shading nets are arranged at intervals of 2m above the broodstock pond. 10 nylon meshes are placed at the bottom of the pond. The water is changed 3 times a day, with a water change volume of 100%. The bottom is sucked every day and the pond is emptied once every 7 days. Broodstock cultivation: The broodstock in the broodstock pond are fed with enhanced feed for 20 days. After 7 days of intensive cultivation, the sexual maturity of the broodstock is checked. When the gonads of male broodstock can squeeze out semen, they are transferred to the breeding pond for standby use. At this time, female broodstock with enlarged abdomen, soft to touch and red genital opening are selected for artificial induced spawning, while broodstock that are not sexually mature continue to be intensively cultivated; Artificial induction of labor: After the abdomen of the broodstock is obviously swollen, calculate the fullness of the broodstock, fullness = body weight (g) / [body length (cm)]³ × 100%. When the fullness is greater than 3%, artificial induction of labor is performed, and the back muscle injection is performed. The dosage of oxytocin for male fish is 1 / 3 of that for female fish. If the female fish does not lay eggs after one week, continue to inject, and the male fish does not need to be injected. Each broodstock can be injected twice at most. The dosage of oxytocin HCG for female broodstock is 550IU / kg, diluted to 1ml with normal saline; Natural fertilization and hatching: The artificially induced female and male broodstock are placed in a spawning pond. After the broodstock lay eggs, they are disinfected with a 1 mg / L sodium penicillin seawater solution for 1 hour and then placed in a larval breeding pond for hatching to obtain blue round tuna fry. The water temperature of the larval breeding pond is 28°C and the hatching time is 48 hours. The salinity of the water in the larval breeding pond is 32‰, the pH value is between 7.8, and the dissolved oxygen in the water is 6.00 mg / L.

[0033] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for artificial breeding of bluefin trevally, characterized in that: include: Parent fish cultivation: Select wild blue round fish for cage culture and feed them with culture feed; Broodstock transplantation: When the weight of broodstock reaches the standard of more than 200g / tail for female fish and more than 150g / tail for male fish, select roundtails with strong physique, active movements, strong resistance and no external injuries on the body surface as broodstock, and transfer the broodstock from the net cage to the broodstock pond. The ratio of female broodstock to male broodstock is 2-2.5:1; Broodstock cultivation: The broodstock in the broodstock pond are fed with enhanced feed for 15-20 days. After 7 days of intensive cultivation, the sexual maturity of the broodstock is checked. When the gonads of male broodstock can squeeze out semen, they are transferred to the breeding pond for standby use. At this time, female broodstock with enlarged abdomen, soft to touch and red genital opening are selected for artificial induced spawning, while broodstock that are not sexually mature continue to be intensively cultivated; Artificial induction of labor: After the abdomen of the broodstock is obviously swollen, calculate the fullness of the broodstock, fullness = body weight (g) / [body length (cm)]³ × 100%. When the fullness is greater than 3%, artificial induction of labor is performed by back muscle injection. The dosage of oxytocin for male fish is 1 / 3 of that for female fish. If the female fish does not lay eggs after one week, continue to inject, but the male fish does not need to be injected. Each broodstock can be injected up to 2 times; Natural fertilization and hatching: The artificially induced female and male broodstock are placed in a spawning pond. After the broodstock lay eggs, they are disinfected with a 1 mg / L sodium penicillin seawater solution for 1 hour and then placed in a larval breeding pond for hatching to obtain blue round fish fry.

2. The artificial breeding method of blue trevally according to claim 1, characterized in that: In the broodstock cultivation step, the breeding bait consists of shrimp, water, kelp powder, spirulina powder and yeast, and the weight ratio of the shrimp, water, kelp powder, spirulina powder and yeast is 100:70:2:1:

1. A small amount of water is added to the shrimp compound feed particles to make them sticky, and then the kelp powder and spirulina powder are mixed evenly, and then air-dried to make particles to form the breeding bait.

3. The artificial breeding method of blue trevally according to claim 1, characterized in that: In the broodstock growing step, the stocking density of cage culture is 6-10 fish / m3, the daily feeding amount of breeding feed is 1%-2% of the fish body weight, and the feeding is 2-3 times a day. The breeding time is 3-6 months.

4. The artificial breeding method of blue trevally according to claim 1, characterized in that: In the broodstock transplantation step, the bottom area of ​​the broodstock pond is 15-20m², the depth of the broodstock pond is 1.8-2.2m, 80-90 broodstock are placed in each pond, 15-18 air stones are placed on the bottom of the broodstock pond, shading nets are arranged at intervals 1.6-2m above the broodstock pond, 8-10 nylon meshes are placed on the bottom of the pond, the water is changed 1-3 times a day, the water change amount is 100%, the bottom is sucked every day, and the pond is emptied once every 5-7 days.

5. The artificial breeding method of blue trevally according to claim 1, characterized in that: In the broodstock transplantation step, the best time to catch the broodstock is early in the morning, without feeding on the same day, with a transport density of 60-75 fish / m³, a water temperature of 17°C-20°C, and a transport time of less than 6 hours.

6. The artificial breeding method of blue trevally according to claim 1, characterized in that: In the broodstock cultivation step, the fortified bait consists of chilled fish, eel meal, probiotics, multivitamins, fish oil, vitality and yeast, and the mass ratio of the chilled fish, eel meal, probiotics, multivitamins, fish oil, vitality and yeast is 400:800:1:1:1:1:

1.

7. The artificial breeding method of blue trevally according to claim 1, characterized in that: In the artificial induction step, the dosage of HCG used for female broodstock is 450-550 IU / kg, which is diluted to 0.5-1 ml with physiological saline.

8. The artificial breeding method of blue trevally according to claim 1, characterized in that: In the steps of natural fertilization and hatching, the water temperature of the larvae rearing pool is 25°C-28°C and the hatching time is 24-48 hours.

9. The artificial breeding method of blue trevally according to claim 1, characterized in that: In the natural fertilization and hatching steps, the salinity of the water body is 30-32‰, the pH value is between 7.5-7.8, and the dissolved oxygen of the water body is 4.00-6.00 mg / L.

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