Bifidobacterium symbiont as well as culture method and application thereof

Abstract

The invention discloses a bifidobacterium symbiont as well as a culture method and application thereof, and belongs to the technical field of probiotics. The invention provides a bifidobacterium symbiont. The bifidobacterium symbiont is prepared from bifidobacterium, leuconostoc mesenteroides and pediococcus acidilactici. In the growth and metabolism process, metabolites of the three kinds of bacteria can provide sufficient energy for growth and reproduction of the three kinds of bacteria, the three kinds of bacteria are mutually beneficial and symbiotic, and co-growth of the three kinds of bacteria is synergistically promoted. Meanwhile, the leuconostoc mesenteroides and the pediococcus acidilactici provide a suitable environment for the growth of the bifidobacteria by consuming oxygen in a culture system, so that strict anaerobic environment control during bifidobacteria culture is avoided, the production cost is saved, and a foundation is laid for the production and utilization of probiotics.

Description

Technical Field

[0001] The invention belongs to the technical field of probiotics, and in particular relates to a bifidobacterium symbiont and a culture method and application thereof. Background Art

[0002] Bifidobacterium is a Gram-positive bacterium that is widely found in the digestive tract, vagina and oral cavity of humans and animals. It is one of the important members of the intestinal flora of humans and animals. As a physiological beneficial bacterium, bifidobacterium has many important physiological functions such as biological barrier, regulating intestinal flora, inhibiting the growth of pathogens, nutritional effects, anti-tumor effects, immune enhancement effects, improving gastrointestinal function, anti-aging, etc. for human health, and is widely used in medical care, health care and food. However, bifidobacterium is an obligate anaerobe, with extremely poor resistance to aerobic environment, and has high requirements for nutrition and culture conditions. Even in the most suitable environment, the survival time is short. Therefore, how to increase the yield of bifidobacterium in a short time has become a key technology. At present, the commonly used method is to screen bifidobacterium strains on a large scale, or to domesticate existing strains in order to obtain strains with excellent growth and reproduction performance, but this method has a large amount of preliminary work and is highly accidental, and it is difficult to adapt to the needs of related industries. Therefore, it is particularly important to provide a method that can promote the growth of bifidobacteria and increase the yield of bifidobacteria. Summary of the invention

[0003] In view of this, the object of the present invention is to provide a bifidobacterium symbiont that can synergize and significantly promote the growth of bifidobacteria.

[0004] Another object of the present invention is to provide a method for culturing the bifidobacterium symbiont.

[0005] Another object of the present invention is to provide an application of the bifidobacterium symbiont or the culture method in preparing a probiotic-rich product.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The invention provides a bifidobacterium symbiont, which consists of bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici; the ratio of live bacteria of the bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici is 1-5:1-5:1-5.

[0008] Preferably, the Leuconostoc mesenteroides is Leuconostoc mesenteroides CICC 22264; and the Pediococcus acidilactici is Pediococcus acidilactici CICC 10344.

[0009] Preferably, the bifidobacterium is Bifidobacterium longum, Bifidobacterium adolescentis, Bifidobacterium animalis or Bifidobacterium bifidum.

[0010] Preferably, the ratio of live bacteria of Bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici is 2-4:3-5:1-2.

[0011] The present invention also provides a method for culturing the symbiont, comprising the following steps: inoculating bifidobacteria, Leuconostoc mesenteroides and Pediococcus acidilactici into a liquid culture medium for closed culture.

[0012] Preferably, based on 1L, the liquid culture medium includes the following raw materials: 10-15g of peptone, 15-20g of glucose, 5-10g of yeast extract, 4-6g of sodium chloride, 1-2g of dipotassium hydrogen phosphate, 1-2g of potassium dihydrogen phosphate, 0.2-0.5g of magnesium sulfate, 0.05-0.1g of manganese chloride, and the balance is water.

[0013] Preferably, the initial pH of the liquid culture medium is 6-7.

[0014] Preferably, the culture conditions are: initial temperature 28-34° C. for 1-3 h; temperature raised to 35-40° C. for 16-30 h.

[0015] The present invention also provides an application of the symbiont or the culturing method in preparing a product rich in probiotics.

[0016] Preferably, the product includes food, medicine or feed.

[0017] Beneficial effects of the present invention:

[0018] The present invention provides a bifidobacterium symbiont, which is composed of bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici. During the growth and metabolism process, the metabolites of the three bacteria can provide sufficient energy for the growth and reproduction of each other, and the three bacteria can mutually benefit and symbiotically promote the common growth of the three bacteria. After cultivation, the number of viable bifidobacteria can reach 1.31×10 8 ~2.01×10 9 cfu / mL, the number of live bacteria of Leuconostoc mesenteroides can reach 2.37×10 7 ~4.36×10 8 cfu / mL, the number of live bacteria of Pediococcus acidilactici can reach 9.77×10 8 ~4.73×10 9 The three bacteria in the bifidobacterium symbiont of the present invention promote each other's growth, and at the same time, Leuconostoc mesenteroides and Pediococcus acidilactici provide a suitable environment for the growth of bifidobacteria by consuming oxygen in the culture system, avoiding the strict control of the anaerobic environment during the cultivation of bifidobacteria, saving production costs, and laying a foundation for the production and utilization of probiotics. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 The viable counts of Bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici at different culture times in Example 1. DETAILED DESCRIPTION

[0020] The present invention provides a bifidobacterium symbiont, which is composed of bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici. The ratio of live bacteria of the bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici is preferably 1-5:1-5:1-5.

[0021] In the present invention, the type of bifidobacterium can be conventionally selected according to actual needs, preferably including Bifidobacterium longum, Bifidobacterium adolescentis, Bifidobacterium animalis or Bifidobacterium bifidum, further preferably Bifidobacterium longum or Bifidobacterium animalis, more preferably Bifidobacterium longum. In the present invention, the Leuconostoc mesenteroides is preferably Leuconostoc mesenteroides CICC 22264; the Pediococcus acidilactici is preferably Pediococcus acidilactici CICC 10344. The ratio of live bacteria of the bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici is further preferably 2-4:3-5:1-2, more preferably 3:5:2.

[0022] In the present invention, the metabolites produced during the growth and metabolism of Bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici can provide sufficient energy for the growth and reproduction of each other, and the three bacteria can coexist mutually beneficially and synergistically promote the common growth of the three bacteria.

[0023] The present invention also provides a method for culturing the symbiont, comprising the following steps: inoculating bifidobacteria, Leuconostoc mesenteroides and Pediococcus acidilactici into a liquid culture medium for closed culture.

[0024] In the present invention, the culture method preferably further comprises preparing seed solutions of Bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici respectively, and inoculating the seed solutions into a liquid culture medium for closed culture. The method for preparing the seed solution is not particularly limited and can be conventionally selected according to actual needs. The viable bacteria concentration of the seed solution is preferably 1×10 5 ~1×10 7 cfu / mL, more preferably 5×10 5 ~5×10 6 cfu / mL, more preferably 1×10 6 cfu / mL. The inoculation amount of the seed solution of bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici can be conventionally selected according to actual needs. As an implementable embodiment, the total inoculation amount of the seed solution of bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici is 3% to 8% of the volume of the liquid culture medium, more preferably 4% to 6%, and more preferably 5%; the ratio of live bacteria of the bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici is preferably 1 to 5:1 to 5:1 to 5, more preferably 2 to 4:3 to 5:1 to 2, and more preferably 3:5:2.

[0025] In the present invention, the type of the liquid culture medium can be conventionally selected according to actual needs. As an optional embodiment, based on 1L, the liquid culture medium preferably includes the following raw materials: peptone 10-15g, glucose 15-20g, yeast extract 5-10g, sodium chloride 4-6g, dipotassium hydrogen phosphate 1-2g, potassium dihydrogen phosphate 1-2g, magnesium sulfate 0.2-0.5g, manganese chloride 0.05-0.1g, and the balance is water; further preferably, based on 1L, the liquid culture medium preferably includes the following raw materials: peptone 12g, glucose 18g, yeast extract 10g, sodium chloride 5g, dipotassium hydrogen phosphate 2g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0.3g, manganese chloride 0.08g, and the balance is water. The initial pH of the liquid culture medium is preferably 6-7, further preferably 6.2-6.7, and more preferably 6.5.

[0026] In the present invention, the culture is a closed culture, and there is no need to deliberately create an anaerobic environment. The culture conditions are preferably: the initial temperature is 28-34 ° C for 1-3 hours; the temperature is raised to 35-40 ° C for 16-30 hours; the initial temperature is further preferably 29-32 ° C, more preferably 30 ° C; the initial culture time is further preferably 1.5-2.5 hours, more preferably 2 hours; the heating temperature is further preferably 36-38 ° C, more preferably 37 ° C; the heating culture time is further preferably 18-28 hours, more preferably 24 hours. Under the initial temperature conditions, it is in the optimal growth temperature range of intestinal mesenteroides, which can enable intestinal mesenteroides to quickly enter the growth and reproduction stage, and a certain number scale can be reached in the early stage of fermentation, which lays the foundation for the subsequent symbiotic fermentation with other strains, and can also quickly consume and utilize oxygen in the system to create conditions for the anaerobic fermentation of bifidobacteria; and the metabolic activities of bifidobacteria and lactic acid Pediococcus at this stage are relatively mild, which is conducive to their adaptation to the new environment and preparation for subsequent growth and reproduction. After the temperature rises, it enters the suitable growth temperature range for Bifidobacterium and Pediococcus acidilactici. At this time, their metabolic activities are enhanced, their growth rates are accelerated, and they can reproduce in large numbers, cooperating with Enterococcus mesenteroides to promote the stability and development of the entire symbiotic culture system.

[0027] The culturing method of the present invention is to inoculate bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici into a liquid culture medium at the same time. Since Leuconostoc mesenteroides and Pediococcus acidilactici are facultative anaerobic bacteria, they can first consume and utilize oxygen in a closed environment, provide conditions for anaerobic fermentation of bifidobacteria, avoid strict control of the anaerobic environment during culturing of bifidobacteria, and save production costs; Leuconostoc mesenteroides has weak ability to synthesize amino acids and vitamins, and some amino acids, vitamins and other nutrients produced by bifidobacteria and Pediococcus acidilactici during the fermentation process can be used by Leuconostoc mesenteroides, and vice versa, thereby forming a nutrient sharing mechanism and promoting the common growth of the three bacteria; at the same time, through co-culturing, the three bacteria may be induced to produce some metabolites with special physiological functions, thereby improving the probiotic effect of the entire culturing system, and the interaction between the three bacteria can also enhance their resistance to environmental factors. When environmental temperature, pH and other conditions change, the three bacteria can adjust their own metabolic activities and physiological states to adapt to the temperature change together, thereby improving the stability and adaptability of the entire culturing system.

[0028] The present invention also provides an application of the symbiont or the culturing method in preparing a product rich in probiotics.

[0029] In the present invention, the category of the product rich in probiotics is not particularly limited, including food, medicine or feed, etc. The probiotics in the product may only include the bifidobacterium symbionts, or may include other probiotic components; the product may also include corresponding auxiliary materials selected according to the product type, and the types of auxiliary materials are not particularly limited and can be conventionally selected according to actual needs. The product rich in probiotics can be used to supplement the probiotic content of the human or animal body, regulate intestinal flora, promote digestion, moisturize the intestines and laxatives, etc.

[0030] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0031] In the following embodiments, unless otherwise specified, all of them are conventional methods.

[0032] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0033] In the following examples, Bifidobacterium longum CICC 6202, Leuconostoc mesenteroides CICC 22264 and Pediococcus acidilactici CICC 10344 were purchased from China Industrial Microbiological Culture Collection Center; Bifidobacterium animalis CGMCC 1.1852 and Bifidobacterium bifidum ATCC 11863 were purchased from Shanghai Collection Biotechnology Center.

[0034] The liquid culture medium in the following examples contains: 12 g / L peptone, 18 g / L glucose, 10 g / L yeast extract, 5 g / L sodium chloride, 2 g / L dipotassium hydrogen phosphate, 1.5 g / L potassium dihydrogen phosphate, 0.3 g / L magnesium sulfate, and 0.08 g / L manganese chloride, and water is added to make the volume to 1 L.

[0035] Example 1

[0036] A bifidobacterium symbiont, the symbiont is composed of Bifidobacterium longum CICC 6202, Leuconostoc mesenteroides CICC22264 and Pediococcus acidilactici CICC 10344; the ratio of live bacteria of Bifidobacterium longum CICC 6202, Leuconostoc mesenteroides CICC 22264 and Pediococcus acidilactici CICC 10344 is 3:5:2.

[0037] The method for culturing the bifidobacterium symbiont is as follows:

[0038] The seed solutions of Bifidobacterium longum, Leuconostoc mesenteroides, and Pediococcus acidilactici were prepared respectively, and the live bacterial concentration of the seed solutions was 1×10 6 cfu / mL; the seed solutions of Bifidobacterium longum, Leuconostoc mesenteroides and Pediococcus acidilactici were inoculated into the liquid culture medium at an inoculum size of 1.5%, 2.5% and 1% (v / v), respectively, the initial pH of the culture medium was adjusted to 6.5, and the culture was closed. The culture conditions were: initial temperature of 30°C for 2h, then the temperature was raised to 37°C for 24h.

[0039] Example 2

[0040] A Bifidobacterium symbiont:

[0041] The difference from Example 1 is that Bifidobacterium animalis CGMCC1.1852 replaces Bifidobacterium longum CICC6202, and the culturing method is the same as Example 1.

[0042] Example 3

[0043] A Bifidobacterium symbiont:

[0044] The difference from Example 1 is that Bifidobacterium bifidum ATCC 11863 is used to replace Bifidobacterium longum CICC6202, and the culturing method is the same as that of Example 1.

[0045] Example 4

[0046] A bifidobacterium symbiont, the symbiont is composed of Bifidobacterium longum CICC 6202, Leuconostoc mesenteroides CICC22264 and Pediococcus acidilactici CICC 10344; the ratio of live bacteria of Bifidobacterium longum CICC 6202, Leuconostoc mesenteroides CICC 22264 and Pediococcus acidilactici CICC 10344 is 4:3:1.

[0047] The method for culturing the bifidobacterium symbiont is as follows:

[0048] The same method as in Example 1 was used to prepare seed solutions of Bifidobacterium longum, Leuconostoc mesenteroides and Pediococcus acidilactici; the seed solutions of Bifidobacterium longum, Leuconostoc mesenteroides and Pediococcus acidilactici were inoculated into liquid culture medium at inoculation amounts of 2.5%, 1.875% and 0.625% (v / v), respectively, and the initial pH of the culture medium was adjusted to 6. The culture was closed and the culture conditions were as follows: the initial temperature was 28° C. for 2.5 h, and the temperature was raised to 38° C. for 28 h.

[0049] Example 5

[0050] A bifidobacterium symbiont, the symbiont is composed of Bifidobacterium longum CICC 6202, Leuconostoc mesenteroides CICC22264 and Pediococcus acidilactici CICC 10344; the ratio of live bacteria of Bifidobacterium longum CICC 6202, Leuconostoc mesenteroides CICC 22264 and Pediococcus acidilactici CICC 10344 is 2:4:2.

[0051] The method for culturing the bifidobacterium symbiont is as follows:

[0052] The same method as in Example 1 was used to prepare seed solutions of Bifidobacterium longum, Leuconostoc mesenteroides and Pediococcus acidilactici; the seed solutions of Bifidobacterium longum, Leuconostoc mesenteroides and Pediococcus acidilactici were inoculated into liquid culture medium at inoculation amounts of 1.25%, 2.5% and 1.25% (v / v), respectively, and the initial pH of the culture medium was adjusted to 7, and closed culture was performed under the following culture conditions: initial temperature of 29° C. for 1.5 h, and then the temperature was raised to 36° C. for 18 h.

[0053] Comparative Example 1

[0054] A bifidobacterium symbiont, which is composed of Bifidobacterium longum CICC 6202 and Leuconostoc mesenteroides CICC22264; the ratio of live bacteria of Bifidobacterium longum CICC 6202 and Leuconostoc mesenteroides CICC 22264 is 3:5.

[0055] The method for culturing the bifidobacterium symbiont is as follows:

[0056] The same method as in Example 1 was used to prepare seed solutions of Bifidobacterium longum and Leuconostoc mesenteroides; the seed solutions of Bifidobacterium longum and Leuconostoc mesenteroides were inoculated into liquid culture medium at inoculation amounts of 1.875% and 3.125% (v / v), respectively, and the initial pH of the culture medium was adjusted to 6.5, and closed culture was performed under the following culture conditions: initial temperature of 30° C. for 2 h, and then the temperature was raised to 37° C. for 24 h.

[0057] Comparative Example 2

[0058] A bifidobacterium symbiont, the symbiont is composed of Bifidobacterium longum CICC 6202 and Pediococcus acidilactici CICC10344; the ratio of live bacteria of Bifidobacterium longum CICC 6202 and Pediococcus acidilactici CICC 10344 is 3:2.

[0059] The method for culturing the bifidobacterium symbiont is as follows:

[0060] The same method as in Example 1 was used to prepare Bifidobacterium longum and Pediococcus acidilactici seed solutions; the Bifidobacterium longum and Pediococcus acidilactici seed solutions were inoculated into liquid culture medium at an inoculum amount of 3% and 2% (v / v), respectively, and the initial pH of the culture medium was adjusted to 6.5, and the culture was closed and the culture conditions were: initial temperature 30° C. for 2 h, and then the temperature was raised to 37° C. for 24 h.

[0061] Comparative Example 3

[0062] The same method as in Example 1 was used to prepare Bifidobacterium longum CICC 6202 seed solution; the Bifidobacterium longum seed solution was inoculated into a liquid culture medium at a 5% (v / v) inoculation rate, the initial pH of the culture medium was adjusted to 6.5, and the culture was closed under the following culture conditions: initial temperature 30° C. for 2 h, and then the temperature was raised to 37° C. for 24 h.

[0063] Comparative Example 4

[0064] The same method as in Example 1 was used to prepare a seed solution of Leuconostoc mesenteroides CICC 22264; the seed solution of Leuconostoc mesenteroides was inoculated into a liquid culture medium at a 5% (v / v) inoculation rate, the initial pH of the culture medium was adjusted to 6.5, and the culture was closed under the following culture conditions: an initial temperature of 30° C. for 2 h, and then the temperature was raised to 37° C. for 24 h.

[0065] Comparative Example 5

[0066] The same method as in Example 1 was used to prepare a seed solution of Pediococcus acidilactici CICC 10344; the seed solution of Pediococcus acidilactici was inoculated into a liquid culture medium at a 5% (v / v) inoculation amount, the initial pH of the culture medium was adjusted to 6.5, and the culture was closed under the following culture conditions: an initial temperature of 30° C. for 2 h, and then the temperature was raised to 37° C. for 24 h.

[0067] Test Example 1

[0068] The viable counts of the three bacteria in the culture system of Example 1 were measured at 0 h, 2 h, 4 h, 8 h, 12 h, 16 h, 20 h, 24 h and 26 h after the start of culture. The results are as follows: Figure 1 shown.

[0069] Depend on Figure 1It can be seen that there is no obvious inhibitory effect on the growth of the three strains. In the early stage of culture, Leuconostoc mesenteroides grew rapidly and entered a stable growth period after 12 hours, with the highest number of viable bacteria reaching 3.15×10 8 cfu / mL; the number of live bacteria of Pediococcus acidilactici was the highest, and the stable period was reached after 20h of cultivation, with the highest number of live bacteria reaching 4.75×10 9 cfu / mL; incubation time 0-2h, Bifidobacterium grew slowly, then entered a rapid growth phase, and entered a stable growth phase after 16h, with the highest viable count reaching 2.01×10 9 cfu / mL. This indicates that Bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici can coexist and promote each other's growth and proliferation.

[0070] Test Example 2

[0071] After the culture was completed, the number of viable bacteria of each species in the culture systems of Examples 1-5 and Comparative Examples 1-5 was measured respectively. The results are shown in Table 1.

[0072] Table 1 Viable bacteria count (cfu / mL)

[0073]

[0074]

[0075] From the results in Table 1, it can be seen that after the symbiotic culture of Bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici, the number of viable Bifidobacterium reached 1.31×10 8 ~2.01×10 9 cfu / mL, the number of live Leuconostoc mesenteroides reached 2.37×10 7 ~4.36×10 8 cfu / mL, the number of live bacteria of Pediococcus acidilactici reached 9.77×10 8 ~4.73×10 9 cfu / mL; when only Bifidobacterium and Leuconostoc mesenteroides were used for symbiotic culture, under the same culture conditions, although both could grow normally, their growth rates were significantly reduced, and the concentration of live bacteria decreased significantly; when only Bifidobacterium and Pediococcus acidilactici were used for symbiotic culture, under the same culture conditions, although Bifidobacterium could grow normally, Pediococcus acidilactici would competitively inhibit its growth, resulting in slower growth of Bifidobacterium and lower concentration of live bacteria; when Bifidobacterium, Leuconostoc mesenteroides or Pediococcus acidilactici were cultured alone, since the system was not a strictly anaerobic environment and was not suitable for the growth of Bifidobacterium, a large number of Bifidobacteria died, and although Leuconostoc mesenteroides and Pediococcus acidilactici could grow normally, their growth rates were slow and the number of live bacteria was low.

[0076] The above results show that the three bacteria in the bifidobacterium symbiont of the present invention have a growth-promoting effect on each other. At the same time, Leuconostoc mesenteroides and Pediococcus acidilactici provide a suitable environment for the growth of bifidobacteria by consuming oxygen in the culture system, avoiding the strict control of the anaerobic environment during the cultivation of bifidobacteria and saving production costs.

[0077] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A bifidobacterium symbiont, characterized in that The symbiont is composed of bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici; the ratio of live bacteria of the bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici is 1-5:1-5:1-5.

2. The symbiotic organism according to claim 1, characterized in that: The Leuconostoc mesenteroides is Leuconostoc mesenteroides CICC22264; the Pediococcus acidilactici is Pediococcus acidilactici CICC 10344.

3. The symbiotic organism according to claim 1, characterized in that: The bifidobacterium is Bifidobacterium longum, Bifidobacterium adolescentis, Bifidobacterium animalis or Bifidobacterium bifidum.

4. The symbiotic organism according to claim 1, characterized in that: The ratio of the live bacteria of the bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici is 2-4:3-5:1-2.

5. The method for cultivating a symbiotic organism according to any one of claims 1 to 4, characterized in that: The method comprises the following steps: inoculating bifidobacterium, Leuconostoc mesenteroides and Pediococcus acidilactici into a liquid culture medium for closed culture.

6. The method according to claim 5, characterized in that Based on 1L, the liquid culture medium includes the following raw materials: 10-15g of peptone, 15-20g of glucose, 5-10g of yeast extract, 4-6g of sodium chloride, 1-2g of dipotassium hydrogen phosphate, 1-2g of potassium dihydrogen phosphate, 0.2-0.5g of magnesium sulfate, 0.05-0.1g of manganese chloride, and the balance is water.

7. The method according to claim 5, characterized in that The initial pH of the liquid culture medium is 6-7.

8. The method according to claim 5, characterized in that The culture conditions are as follows: initial temperature of 28-34° C. for 1-3 h; temperature raised to 35-40° C. for 16-30 h.

9. Use of the symbiotic organism according to any one of claims 1 to 4 or the method according to any one of claims 5 to 8 in the preparation of a probiotic-rich product.

10. The use according to claim 9, characterized in that: The product includes food, medicine or feed.

Images