Method for identifying menstrual blood and peripheral blood by using expression quantity relationship of miRNA based on RNU6b as reference gene
By screening out three miRNAs, miR-451a, miR-144-5p and miR-203-3p, and using RNU6b as the internal reference gene, an efficient and accurate method of distinguishing menstrual blood and peripheral blood was established, solving the problem of insufficient identification accuracy in the prior art and providing a reliable forensic physical evidence tool.
CN119639908APending Publication Date: 2025-03-18杨天畅
0 Cites 0 Cited by
Patent Information
- Application Number
- CN202411815233.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-11
- Publication Date
- 2025-03-18
AI Technical Summary
Technical Problem
The prior art is difficult to efficiently and accurately identify peripheral blood and menstrual blood. The traditional methods are highly subjective and insufficiently accurate.
Method used
Based on RNU6b as the internal reference gene, three miRNAs, miR-451a, miR-144-5p and miR-203-3p, were screened out. Through high-throughput sequencing and real-time fluorescence quantitative PCR technology, a method for efficient and accurate identification of menstrual blood and peripheral blood was established.
Benefits of technology
It achieves efficient and accurate identification of menstrual blood and peripheral blood, and provides a reliable forensic physical evidence body fluid identification tool, which improves the scientificity and accuracy of identification.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention discloses a method for identifying menstrual blood and peripheral blood by using an expression quantity relationship of miRNA based on RNU6b as a reference gene, and relates to the technical field of biology. According to the invention, three miRNAs (miR-144-5p, miR-203-3p and miR-451a) with high expression difference are screened from vaginal blood and peripheral blood on the basis of a high-throughput sequencing technology for the first time, and the difference of screening indexes is further verified in other three body fluids (semen, saliva and vaginal secretion); meanwhile, six reference genes (RNU6b, U6, miR-103a-3p, miR-191-5p, miR-92a-3p and miR-484) are screened to evaluate comparison results, and the RNU6b with the highest stability is finally selected as the reference gene, so that an efficient and accurate identification method system is finally established, or the method will become a powerful tool for body fluid plaque tissue sources of forensic physical evidence, and can be used as a kit for identifying body fluid plaque tissue sources of forensic physical evidence. And more scientific and reasonable auxiliary evidences are provided for qualitative and detection of cases.
Need to check novelty before this filing date? Find Prior Art