Wheat drought resistance related haplotype molecular marker, primer, kit and application thereof

By developing haplotype molecular markers in the promoter region of the TaXTH-2B gene in wheat, the problem of difficult to identify excellent haplotypes of wheat drought resistance genes in the prior art is solved, and effective screening and identification of wheat varieties with high drought resistance index has been achieved, which significantly improves the efficiency of genetic improvement of wheat drought resistance.

CN119639938AActive Publication Date: 2025-03-18HENAN AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202411709166.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-27
Publication Date
2025-03-18
Estimated Expiration
2044-11-27

AI Technical Summary

Technical Problem

The prior art is difficult to effectively identify and screen excellent haplotypes of TaXTH-2B gene promoter in wheat, resulting in inefficient genetic improvement of drought resistance in wheat.

Method used

A haplotype molecular marker related to wheat drought resistance was developed, located in the promoter region of the TaXTH-2B gene. By designing specific primers, haplotypes of the TaXTH-2B gene promoter of different wheat varieties are used to identify and screen wheat varieties with high drought resistance index.

Benefits of technology

Through this molecular marker, the TaXTH-2B Hap II haplotype wheat variety with a high drought resistance index can be effectively identified. It has higher promoter activity than the Hap I haplotype, significantly improving the screening efficiency of wheat drought resistance varieties.

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Abstract

The invention discloses haplotype molecular markers related to wheat drought resistance, primers, a kit and application of the haplotype molecular markers, and aims to solve the technical problem that molecular markers capable of identifying excellent haplotypes of TaXTH-2B gene promoters are lacked at present. It is confirmed that the TaXTH-2B gene promoter has two haplotypes, and the Hap II haplotype is an excellent haplotype endowing wheat with a high drought resistance index and has high promoter activity; meanwhile, the invention develops molecular marker primers for distinguishing the two haplotypes, and the molecular marker primers have wide application prospects in the aspects of identifying and screening bred wheat varieties or breeding intermediate generation materials, assisting in selecting wheat materials with high drought resistance indexes and the like.
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