Culture medium for VBNC bacteria and preparation method of culture medium

By providing a VBNC bacterial culture medium containing a variety of nutrients and growth promoters, the problem of limited effect on resuscitation of VBNC state tuberculosis in the prior art is solved, and the effect of significantly improving the resuscitation rate and growth rate is achieved.

CN119685199APending Publication Date: 2025-03-25ZHEJIANG WEIPEIFANG BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202411672763.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-21
Publication Date
2025-03-25

AI Technical Summary

Technical Problem

The existing culture medium has limited effect on tuberculosis in resuscitation of VBNC state, and it is difficult to effectively cultivate and detect under adverse environmental conditions.

Method used

Provided is a culture medium for VBNC bacteria, which includes bovine serum albumin, glucose, Dubos broth base, glycerin, Tween, indole-3-acetic acid, antioxidants, amino acid mixtures, cytokines, antimicrobial peptides, transfer factors, IDR peptides, microbial metabolites and water. Through reasonable composition ratios and preparation methods, the resuscitation and growth of bacteria are promoted.

Benefits of technology

It significantly improves the resuscitation and growth rate of VBNC bacteria, provides new methods for disease detection and treatment, adapts to VBNC bacteria resuscitation under different environmental conditions, and has a wide range of application prospects.

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Abstract

The invention relates to the field of microbial culture, in particular to a culture medium for VBNC bacteria and a preparation method thereof.The culture medium for the VBNC bacteria is prepared from bovine serum albumin, glucose, Dubos broth base, glycerin, Tween, indole-3-acetic acid, an antioxidant, an amino acid mixture, cell factors, antibacterial peptide, transfer factors, IDR peptide, microbial metabolites and water. The recovery rate and the growth rate of VBNC bacteria are remarkably improved, and a new method is provided for detection and treatment of related diseases; through reasonable component proportion, the culture medium can provide various nutrient substances required by growth of bacteria and promote recovery and proliferation of the bacteria.
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