Mustard SNP (Single Nucleotide Polymorphism) molecular marker combination and application thereof

By using 1694 SNP molecular marker combinations and multiple PCR+ second-generation sequencing technology, the problems of low efficiency and poor accuracy of traditional mustard breeding methods are solved, and efficient and accurate detection and identification of mustard breeding materials are achieved, which improves breeding efficiency and the accuracy of variety selection.

CN119685515AInactive Publication Date: 2025-03-25HUNAN AGRI UNIV +2
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Patent Information

Application Number
CN202411992708.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-31
Publication Date
2025-03-25
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

Traditional mustard breeding methods rely on phenotypic selection, resulting in low breeding efficiency, inaccurate judgment criteria, strong subjectivity, and susceptible to environmental and cultivation conditions, with poor accuracy and stability.

Method used

Using 1694 SNP molecular markers combinations, combined with multiple PCR and second-generation sequencing technology, primer sets and/or probes for detecting the combination of SNP molecular markers of mustard greens were developed, and applied in liquid phase chips and kits for identification of mustard greens germplasm resources, genetic diversity assessment, variety purity and authenticity identification, etc.

Benefits of technology

It has achieved a high-quality and high polymorphic genome-wide marker combination, with high sensitivity and resolution, is not affected by environmental conditions, and has accurate detection results, repeatability and stability. It is suitable for background detection of mustard breeding materials and has wide application universality.

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Abstract

The invention discloses a mustard SNP (Single Nucleotide Polymorphism) molecular marker combination and application thereof. The mustard SNP molecular marker combination is composed of 1694 SNP molecular markers, and the physical positions of the 1694 SNP molecular markers are determined based on sequence alignment of a mustard reference genome Bju.gene. According to the scheme, the mustard SNP molecular marker combination marker is a co-dominant marker, and has the characteristics of high quality, single copy, high polymorphism, high specificity, high sensitivity and high resolution; the marking is not influenced by environmental conditions, seeds or any type of plant tissues can be used, the detection result is accurate, and the repeatability and the stability are good; different detection laboratories and different data results can be compared and verified, and the data has universal comparability, can be accurately used for background detection of mustard breeding materials, and has wide application universality.
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