Nalfurafine artificial hapten and artificial antigen as well as preparation method and application of nalfurafine artificial hapten and artificial antigen

By introducing a connecting arm on nafuraphine and coupling it with bovine serum protein, the problem of lack of immunogenicity of small molecule compounds is solved, and an efficient preparation of anti-nafuraphine polyclonal antibodies is achieved for rapid and accurate detection of nafuraphine.

CN119707992AActive Publication Date: 2025-03-28HANGZHOU TONGZHOU BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202411891027.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-20
Publication Date
2025-03-28
Estimated Expiration
2044-12-20

AI Technical Summary

Technical Problem

The prior art is difficult to detect nafuraphine quickly and accurately, mainly due to the lack of immunogenicity of small-molecular compounds, which cannot directly induce specific antibodies in animal organisms.

Method used

By introducing a linking arm at the phenolic hydroxyl position of nafuraphine, a nafuraphine artificial hapten is generated and coupled to bovine serum protein to form a nafuraphine artificial antigen, and the proliferation and differentiation of B cells are induced by T cell epitope to produce specific antibodies.

Benefits of technology

It has achieved efficient preparation of anti-nafuraphine polyclonal antibodies with high affinity, high sensitivity and strong specificity, which can be used for rapid and accurate immunoassay and analysis of nafuraphine.

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Abstract

The invention belongs to the technical field of biochemical engineering, and particularly relates to a nalfurafine artificial hapten, an artificial antigen as well as a preparation method and application of the nalfurafine artificial hapten and the artificial antigen. The artificial hapten of the nalfurafine retains the characteristic structure of the nalfurafine to the greatest extent, has an active group capable of being coupled with carrier protein, and can be used as an antigenic determinant; the further prepared nalfurafine artificial antigen can be immunized to obtain an anti-nalfurafine polyclonal antibody with high affinity, high sensitivity and strong specificity, the titer of immune serum obtained by immunizing New Zealand white rabbits is up to 1: 128000, and the nalfurafine artificial antigen can be used for rapid and accurate immunodetection and immunoassay of nalfurafine.
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