Bacterial surface modification strategy based on gas shearing

Through a bacterial surface modification strategy based on gas shear, the problems of bacterial surface modification uniformity and high-throughput preparation in the prior art are solved, and the regulation of bacterial number and drug delivery efficiency are improved.

CN119709719APending Publication Date: 2025-03-28GUANGZHOU MEDICAL UNIV
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Patent Information

Application Number
CN202411624917.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-14
Publication Date
2025-03-28

AI Technical Summary

Technical Problem

Existing bacterial surface modification methods are difficult to achieve uniformity and high-throughput preparation, and cannot effectively regulate the number of bacteria and the thickness of the surface modification layer.

Method used

Bacterial surface modification strategy based on gas shear is adopted, bacterial surface modification is performed through gas shearing method, the number of modified bacteria is regulated using gas flow rate, and uniform modification is achieved through compound A (such as sodium alginate or sodium carboxymethylcellulose).

Benefits of technology

The uniform modification of bacterial surface, high-throughput preparation and bacterial number regulation are achieved, and new bacterial surface modification methods are provided, which improves the efficiency of drug delivery and disease treatment.

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Abstract

The invention relates to the technical field of biological medicines, and discloses a bacterial surface modification strategy based on gas shearing, namely bacterial surface modification by using a gas shearing technology, and the strategy specifically comprises the following steps: S1, culturing a target strain overnight, and adjusting OD600 to 1.0; s2, centrifuging the bacterial liquid, re-suspending with a compound A, blowing and beating the uniformly mixed mixture, filling the mixture into an injector, and placing the injector on an injection pump; s3, connecting a coaxial needle head with a gas cylinder and an injector filled with the mixture through hoses in the direction perpendicular to the receiving domain; and S4, opening the gas cylinder to adjust the flow velocity, and carrying out bacterial surface modification. The invention provides a bacterial surface modification strategy based on gas shearing, i.e., bacterial surface modification is carried out by using a gas shearing method, and the strategy can realize uniform modification and high-throughput preparation of the bacterial surface and also can realize regulation and control of the number of bacteria. The strategy provides a new method for surface modification of bacteria, and provides a new thought for drug delivery with bacteria as a carrier and disease treatment.
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