Recombinant nucleotide fragment combination containing multiple rice disease and pest resistance genes as well as detection primer and application of recombinant nucleotide fragment combination
By developing the combination of recombinant nucleotide fragments containing multiple rice pest resistance genes and designing specific primers, the problem of difficulty in effectively binding multiple resistance genes in the prior art is solved, and the comprehensive pest resistance ability of rice is improved.
CN119709772AActive Publication Date: 2025-03-28HUAZHONG AGRI UNIV
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Patent Information
- Application Number
- CN202411538884.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-30
- Publication Date
- 2025-03-28
- Estimated Expiration
- 2044-10-30
AI Technical Summary
Technical Problem
The prior art is difficult to effectively combine multiple rice pest resistance genes to improve the comprehensive pest resistance ability of rice.
Method used
Through molecular marker-assisted selection breeding technology, a combination of recombinant nucleotide fragments containing multiple rice pest resistance genes was developed, and specific primers were designed to detect these recombinant nucleotide fragments.
Benefits of technology
The close linkage of multiple pest and disease resistance genes in rice plants is achieved, the comprehensive pest and disease resistance of rice is improved, and a molecular recognition marker is provided for identification of rice materials containing these recombinant DNA sequences.
✦ Generated by Eureka AI based on patent content.
Abstract
The embodiment of the invention relates to the field of molecular breeding, in particular to a recombinant nucleotide fragment combination containing multiple rice disease and pest resistance genes as well as a detection primer and application of the recombinant nucleotide fragment combination. The recombinant nucleotide fragment combination provided by the invention is selected from at least one of nucleotide sequences as shown in SEQ ID NO.1-8 or complementary sequences of the nucleotide sequences as shown in SEQ ID NO.1-8. The recombinant nucleotide fragment can be used as a specific recognition marker of a major gene and is applied to molecular marker selective breeding; and the gene can be used as a specific molecular recognition marker for identifying a rice material carrying a broad-spectrum resistance gene.
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