Gene ISA1 related to sulfur tolerance character of saccharomyces cerevisiae and application of gene ISA1

By screening and verifying the ISA1 gene related to the sulfur-resistant traits of Saccharomyces cerevisiae, the problem of difficulty in screening the sulfur-resistant traits of Saccharomyces cerevisiae in the prior art was solved, and the effective improvement of the sulfur-resistant traits of Saccharomyces cerevisiae was achieved, and better breeding support was provided for fruit wine brewing.

CN119709786APending Publication Date: 2025-03-28SOUTHWEST FORESTRY UNIVERSITY
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Patent Information

Application Number
CN202411866554.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-18
Publication Date
2025-03-28

AI Technical Summary

Technical Problem

The prior art is difficult to effectively screen and breed Saccharomyces cerevisiae with high sulfur resistance, resulting in Saccharomyces cerevisiae being susceptible to sulfur inhibition during the brewing process of fruit wine, affecting the brewing effect.

Method used

By screening Saccharomyces cerevisiae with large differences in sulfur resistance traits, genome resequencing, relevant QTL sites were localized, and ISA1 genes related to Saccharomyces cerevisiae were specifically screened out, and their function was verified by gene knockout and overexpression.

Benefits of technology

Through the functional confirmation of the ISA1 gene, it was determined that it was related to the sulfur resistance trait of Saccharomyces cerevisiae. After knocking out the gene, the sulfur resistance trait decreased, and the sulfur resistance trait increased after overexpressing the gene, which provided theoretical support for the breeding of Saccharomyces cerevisiae with high sulfur resistance traits.

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Abstract

The invention discloses a gene ISA1 related to the sulfur tolerance character of saccharomyces cerevisiae and application of the gene ISA1, and belongs to the technical field of biology. The nucleotide sequence of the ISA1 gene is as shown in SEQ ID NO. 1. The method comprises the following steps: selecting one or more pairs of saccharomyces cerevisiae parents with great sulfur-tolerant character difference, and hybridizing to obtain a certain number of separated offspring groups. A gene ISA1 related to the sulfur tolerance character of the saccharomyces cerevisiae is screened based on the segregated progeny population. Function confirmation is carried out on the screened ISA1 gene through gene knockout and gene overexpression, it is determined that the ISA1 gene is related to the sulfur-tolerant character of the saccharomyces cerevisiae, the sulfur-tolerant character of the saccharomyces cerevisiae is reduced after the gene is knocked out, and the sulfur-tolerant character of the saccharomyces cerevisiae is improved after the gene is overexpressed. The invention provides a theoretical support for breeding the saccharomyces cerevisiae with high sulfur tolerance.
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