Method for producing L-sorbose through continuous fermentation

Through continuous fermentation, the production of L-sorbate can be solved by using immobilized metabolite nigra and specific crosslinked materials, and the problems of miscellaneous bacteria pollution, high energy consumption and high production costs in the existing processes are solved, and efficient and low-cost sorbate production is achieved.

CN119709908APending Publication Date: 2025-03-28NINGXIA QIYUAN PHARMA
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Patent Information

Application Number
CN202411736292.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-29
Publication Date
2025-03-28

AI Technical Summary

Technical Problem

The existing L-sorbate production process has the risk of miscellaneous bacteria pollution, high energy consumption, high production costs, and long fermentation cycle and cumbersome operation.

Method used

The method of producing L-sorbate was adopted to prepare immobilized metaplastic nigra and ferment in a fermenter. The bacteria were immobilized by cross-linking technology of aminolated graphene, zinc oxide, sodium alginate and polyvinyl alcohol, thereby increasing the stability and metabolism rate of bacteria.

Benefits of technology

It has achieved shortening the fermentation cycle, reducing production costs, improving equipment utilization, saving energy consumption, reducing the total cost of sorbitan fermentation by more than 70%, and ensuring high conversion rate.

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Abstract

The invention discloses a method for producing L-sorbose through continuous fermentation, and relates to the technical field of biology and new medicine. The preparation method comprises the following steps: cross-linking aminated graphene, zinc oxide, sodium alginate and polyvinyl alcohol, and mixing with activated acetobacter aceti liquid to obtain immobilized acetobacter aceti; adding the immobilized acetobacter aceti and a fermentation culture solution into a fermentation tank, and carrying out continuous fermentation to produce L-sorbose; wherein the immobilization of the acetobacter improves the stability of thalli, prolongs the service time of the thalli, shortens the fermentation period, and is convenient to recycle after the fermentation is completed; a continuous fermentation process is adopted, and the purposes of saving energy, reducing consumption and improving quality and efficiency are achieved by simplifying the culture process of the seeds, reducing activation links and culture stages of the seeds and reducing manpower, material resources and energy consumption.
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