Silkworm chrysalis protein capable of resisting fatigue and improving athletic ability

Silkworm pupa protein was extracted by ultrafine pulverization-ultrasonic-enzymatic method, and purified by multi-stage separation and ion exchange chromatography, which solved the problems of purity and biological activity retention of silkworm pupa protein extraction in the prior art, and achieved the effect of efficient extraction and stability improvement.

CN119709923AInactive Publication Date: 2025-03-28BETALIVE (TIANJIN) BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202411989587.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-31
Publication Date
2025-03-28
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

The existing silkworm pupa proteins are difficult to retain biological activity better while improving the extraction purity, and the drying efficiency is limited, making it difficult to retain the active ingredients and functional characteristics of the active peptide.

Method used

The extraction method combined with ultrafine pulverization-ultrasonic-enzymatic method was used to purify by multi-stage separation and ion exchange chromatography, and a combined drying method, including pre-drying and freeze-drying, was used to improve the extraction efficiency and biological activity retention of silkworm pupa protein.

Benefits of technology

It improves the extraction efficiency and purity of silkworm pupa protein, enhances its anti-fatigue, improves motility and storage stability, and ensures the retention of the active ingredients and functional characteristics of the active peptide.

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Abstract

The invention discloses silkworm pupa protein capable of resisting fatigue and improving exercise ability, and relates to the technical field of silkworm pupa protein preparation, and a preparation method of the silkworm pupa protein comprises the following steps: S1, raw material pretreatment: removing heads and feet of fresh silkworm pupa, washing the silkworm pupa with clear water, soaking the silkworm pupa in sterile water for 1-2 hours, stirring at a speed of 30-40 rpm, and taking out the silkworm pupa; the silkworm chrysalis is washed with cold boiled water at the temperature of 20-30 DEG C, the washed silkworm chrysalis is dried at the temperature of 40-50 DEG C, the drying time is 10-30 min, and S2, degreasing treatment is conducted, specifically, grease components in the silkworm chrysalis are removed through multiple degreasing processes. The silkworm chrysalis protein is extracted from silkworm chrysalis in a superfine grinding-ultrasonic wave-enzyme method combined extraction mode, and through the synergistic effect of the three methods, the extraction efficiency of the silkworm chrysalis protein is greatly improved, the silkworm chrysalis protein with higher purity is obtained, the product quality is improved, and meanwhile, the biological activity of the silkworm chrysalis protein can be better reserved; and the functionality of the silkworm pupa protein can be improved.
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