Culture medium, kit and preparation method and application thereof
By preparing a semi-solid culture medium containing specific raw materials for preparation of a kit, the cumbersome and inconvenience of detecting C. perfringens in the prior art are solved, and a fast, convenient and low-cost detection effect is achieved.
Patent Information
- Application Number
- CN202411986714.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-31
- Publication Date
- 2025-03-28
AI Technical Summary
The existing Clostridium perfringens detection methods are cumbersome to operate, the specificity and sensitivity of the test results are low, and the commonly used solid culture medium takes a long time to prepare and is inconvenient to use.
A semi-solid culture medium including peptone, yeast paste powder, beef powder, ammonium ferrous citrate, sodium sulfite and agar powder was proposed. The reagent kit was prepared by mixing it after sterilization and was used to quickly detect Clostridium perfringens. The kit produces black in water bath culture, marking the detection of Clostridium perfringens.
It realizes rapid detection of Clostridium perfringens, with clear and easy to understand, low price, suitable for daily testing and use of each farm, shortening the detection time and eliminating the use of anaerobic equipment.