Indel molecular marker for detecting cold resistance of eggplant and application of Indel molecular marker

By developing Indel molecular markers for eggplant and detecting eggplant genomic DNA using PCR amplification and specific primers, the problem that the prior art cannot effectively detect and identify eggplant cold tolerance, and achieve efficient cold tolerance detection and breeding assisted selection.

CN119710055AActive Publication Date: 2025-03-28JIANGSU ACAD OF AGRI SCI
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Patent Information

Application Number
CN202411505081.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-10-28
Publication Date
2025-03-28
Estimated Expiration
2044-10-28

AI Technical Summary

Technical Problem

The prior art cannot effectively guide the genetic improvement of eggplant's cold resistance, and cannot effectively detect and identify eggplant's cold resistance.

Method used

An Indel molecular marker was developed, and the Indel site obtained after PCR amplification was used to detect eggplant genomic DNA using specific primers to distinguish the differences between cold-resistant materials and cold-sensitive materials.

Benefits of technology

This molecular marker can effectively detect the cold resistance of eggplant, and is used for the molecular marker assisted selection of eggplant cold resistance breeding, which improves breeding efficiency, saves resources and manages costs.

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Abstract

The invention relates to an Indel molecular marker for detecting the cold resistance of eggplants. According to the present invention, the candidate gene significantly related to the eggplant cold resistance is found through the GWAS analysis, and the 17 bp Indel specific molecular marker is developed by carrying out the PCR amplification on the sequence of the candidate gene; the 151st-167th positions of an amplification product of the marker in a cold sensitive material are 17 basic groups of ATATAATCTGATAGTAA, and the 17 basic groups are deleted at the 151st position of the amplification product of the marker in a cold-resistant material. Therefore, the marker can be used for molecular-assisted breeding selection of the cold tolerance of the eggplant.
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