Unwrapped DNA pollutant tracer agent and tracing method

By adjusting the total length of unencapsulated DNA and the length of the core replication segment, combined with the pollutant migration and transformation model, the problem of low penetration rate of DNA tracers in the prior art in soil is solved, and efficiently tracking pollutants of different adsorption rates and decomposition rates in porous media is achieved.

CN119715296AActive Publication Date: 2025-03-28CHINA AGRI UNIV
3 Cites 0 Cited by

Patent Information

Application Number
CN202411782458.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-05
Publication Date
2025-03-28
Estimated Expiration
2044-12-05

AI Technical Summary

Technical Problem

In the prior art, unencapsulated DNA tracers have low penetration rate in soil, while the encapsulated DNA tracers have large particle sizes and are difficult to penetrate soil pores. The tracer recovery rate is low and is not suitable for use in water and soil environments.

Method used

Unwrapped DNA contaminant tracer is used to adjust the total length of double-stranded DNA and the length of the core replication segment to control its adsorption and decomposition characteristics in porous media, and combined with the pollutant migration and transformation model, multi-source tracer of pollutants of different adsorption rates and decomposition rates is achieved.

Benefits of technology

It realizes efficient tracking of soluble chain polymer pollutants with different adsorption rates and decomposition rates in porous media, improves the penetration ability and recovery rate of tracers, and is suitable for pollutant monitoring in water and soil environments.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention provides an unwrapped DNA pollutant tracer agent and a tracing method. The tracer agent comprises the following components: 0.1 to 20 [mu] mol / L of double-stranded DNA, 10 to 50 mmol / L of KBr or NaBr, 0.05 to 0.1 mol / L of Tris, and the pH value is 7.0 to 9.0; the structure of the double-stranded DNA is as follows: a flanking sequence 1-a core replication segment-a flanking sequence 2, the structure of the core replication segment is as follows: a forward primer-Linker 1-a probe-Linker 2-a reverse primer, and the double-stranded DNA does not have homology with the DNA of a known organism. According to the method, the unwrapped DNA tracer is combined with the pollutant migration and transformation model, so that the method has the advantage of tracing soluble chain polymer pollutants with different adsorption rates and decomposition rates in the porous medium, and multi-source tracing of the pollutants in the porous medium can be realized.
Need to check novelty before this filing date? Find Prior Art