Functional feed additive for preventing and treating prawn enterocytozoon hepatopenaei as well as preparation method and application of functional feed additive

Abstract

The invention provides a functional feed additive for preventing and treating prawn enterocytozoon hepatopenaei as well as a preparation method and application of the functional feed additive. The functional feed additive comprises the following components in parts by weight: 5-20 parts of essential oil, 30-60 parts of a plant extract, 0.5-3 parts of eugenol and 1-10 parts of an emulsifier. The functional feed additive provided by the invention can reduce enterocytozoon hepatopenaei infection in the culture process of the penaeus vannamei, improve the immunity of the penaeus vannamei, improve the survival rate and growth performance of the penaeus vannamei, and inhibit and kill enterocytozoon hepatopenaei.

Description

Technical Field

[0001] The invention belongs to the technical field of aquatic feeds, and in particular relates to a functional feed additive for preventing and treating hepatoenterozoa of shrimp, and a preparation method and application thereof. Background Art

[0002] Shrimp hepatocystis is a microsporidia that can infect the hepatopancreas of shrimp, causing shrimp growth retardation. In the early stage of shrimp hepatocystis infection, shrimp eat normally, the intestines are full of food, and there is no large-scale death. When the infection is severe, it causes intestinal inflammation, hepatopancreatic atrophy and discoloration. Due to the lack of obvious identification characteristics, it spreads widely among shrimp, causing heavy losses to the shrimp farming industry and affecting the healthy development of the entire shrimp industry. Due to the parasitic characteristics of shrimp hepatocystis, there is still no effective treatment drug. At present, it is mainly prevented by disinfection in various links of farming, but the effect is not obvious.

[0003] CN202110240755.9 discloses a preparation for preventing and treating shrimp hepatoenteric cyst disease, and its preparation method and use. The preparation is composed of the following raw materials: rosemary oil, fennel oil, talcum powder, and auxiliary materials. The preparation contains by mass percentage: 3.0-3.5% rosemary oil, 1.0-2.0% fennel oil, 15.0-18.0% talcum powder, and 78.0-80.0% auxiliary materials. Talcum powder is feed grade talcum powder, and the auxiliary material is corn starch auxiliary material. The preparation is added to shrimp feed for use, mixed with feed for oral feeding, and 2-3g is added to each kilogram of shrimp feed for 5-7 consecutive days. The compound preparation prepared by the invention is safe for target animals, can effectively inhibit the growth of shrimp hepatoenteric cysts and kill shrimp hepatoenteric cysts, and the preparation process is simple, easy to use, non-toxic, and has no risk of drug residues.

[0004] CN202010006084.5 discloses a drug specifically for killing hepatoenteric cytozoa of shrimp, and its preparation method and application. The drug specifically for killing hepatoenteric cytozoa of shrimp includes the following components in weight percentage: 30-60% albendazole, 10-30% bile acid, 5-15% phyllanthus powder, 10-20% artemisia powder and 5-15% vitamin C. When in use, take the above-mentioned drugs and mix them evenly with the feed at a ratio of 1-2wt%, then spray the diluted adhesive, use it after drying, and use it continuously for more than 2 weeks; it can also be used as a feed additive and added to the feed at an amount of 0.5-1wt%. The drug combination of the invention is reasonable and scientific, and the efficacy of each component works synergistically, which can protect the hepatopancreas of shrimp, kill hepatoenteric cytozoa of shrimp, accelerate the growth of shrimp, and improve the immunity of shrimp.

[0005] CN201610664380.8 discloses a compound preparation for killing shrimp hepatoenteric cysts and its use method. The raw materials of the compound preparation are composed of 4.5-6.0% albendazole, 5.0-8.0% betel nut extract, and 86.0-90.5% corn starch auxiliary material by mass percentage. The betel nut extract is extracted by the laboratory, boiled, concentrated and freeze-dried, and 100g of extract is prepared from 1kg of betel nut. The invention has the following beneficial effects: it is safe for target animals, and the scientific use of the compound preparation can effectively kill shrimp hepatoenteric cysts, meeting the needs of preventing and controlling shrimp hepatoenteric cysts in shrimp farming; the preparation process is simple, easy to use, and has good application prospects.

[0006] However, the above solution still cannot effectively solve the problem caused by shrimp hepatoenteric cysts to shrimp farming. Therefore, how to provide a method for preventing and controlling shrimp hepatoenteric cysts has become an urgent problem to be solved. Summary of the invention

[0007] In view of the shortcomings of the prior art, the purpose of the present invention is to provide a functional feed additive for preventing and treating hepatoenteric cysts of shrimp, and a preparation method and application thereof. The functional feed additive provided by the present invention can reduce hepatoenteric cysts infection during the cultivation of white shrimp, improve the immunity of white shrimp, improve the survival rate and growth performance of white shrimp, and inhibit and kill hepatoenteric cysts.

[0008] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0009] In a first aspect, the present invention provides a functional feed additive for preventing and treating hepatoenteric cysts of shrimp, wherein the functional feed additive comprises, by weight, 5-20 parts of essential oil, 30-60 parts of plant extract, 0.5-3 parts of eugenol and 1-10 parts of emulsifier.

[0010] The number of essential oils can be 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 parts, etc., the number of plant extracts can be 30, 35, 40, 45, 50, 55 or 60 parts, etc., the number of eugenol can be 0.5, 1, 1.5, 2, 2.5 or 3 parts, etc., the number of emulsifiers can be 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 parts, etc., but are not limited to the values ​​listed above, and other values ​​not listed within the above numerical range are equally applicable.

[0011] The above-mentioned functional feed additives can effectively improve the immunity of white shrimp, reduce the infection of hepatoenteric cysts in the breeding process of white shrimp, improve the survival rate and growth performance of white shrimp, and inhibit and kill hepatoenteric cysts through the compound and synergistic effect of essential oils, plant extracts and eugenol.

[0012] Preferably, the essential oil comprises any one of citronella oil, sea buckthorn oil, eucalyptus oil, garlic oil or lemon oil, or a combination of at least two of them, preferably a combination of citronella oil, sea buckthorn oil and lemon oil.

[0013] The above specific essential oil combinations can further enhance the effectiveness of the product.

[0014] Preferably, the mass ratio of the citronella oil, sea buckthorn oil and lemon oil is (1-3):(2-5):(3-6), wherein the number of parts of citronella oil can be 1, 1.5, 2, 2.5 or 3, the number of parts of sea buckthorn oil can be 2, 2.5, 3, 3.5, 4, 4.5 or 5, and the number of parts of lemon oil can be 3, 3.5, 4, 4.5, 5, 5.5 or 6, but are not limited to the values ​​listed above, and other values ​​not listed within the above numerical range are also applicable.

[0015] Preferably, the plant extract comprises any one of a Sophora flavescens extract, an Aloe vera extract, and a Pomegranate peel extract, or a combination of at least two of them, preferably a combination of a Sophora flavescens extract, an Aloe vera extract, and a Pomegranate peel extract.

[0016] The above specific plant extract combination can further enhance the effect of the product.

[0017] Preferably, the mass ratio of the Sophora flavescens extract, the Aloe vera extract and the Pomegranate peel extract is (2-4):(1-3):(3-5), wherein the number of portions of the Sophora flavescens extract can be 2, 2.5, 3, 3.5 or 4, etc., the number of portions of the Aloe vera extract can be 1, 1.5, 2, 2.5 or 3, etc., and the number of portions of the Pomegranate peel extract can be 3, 3.5, 4, 4.5 or 5, etc., but are not limited to the values ​​listed above, and other values ​​not listed within the above numerical range are equally applicable.

[0018] Preferably, the plant extract is obtained by a method comprising the following steps:

[0019] The plant raw material is crushed, then mixed with enzyme and water for ultrasonic extraction, and filtered to obtain the plant extract.

[0020] The above method can extract active ingredients more effectively and improve product effects by performing enzymatic hydrolysis and ultrasonic extraction compared to existing technologies.

[0021] Preferably, the mass ratio of the plant material to water is 1:(25-35), for example 1:25, 1:26, 1:27, 1:28, 1:29, 1:30, 1:31, 1:32, 1:33, 1:34 or 1:35, but is not limited to the above-listed values, and other values ​​not listed within the above numerical range are also applicable.

[0022] Preferably, the enzymes include cellulase, pectinase, neutral protease and α-amylase in a mass ratio of (1.5-2.5):(0.5-1.5):(1.5-2.5):(0.5-1.5), wherein the proportion of cellulase can be 1.5, 1.6, 1.7, .8, 1.9, 2, 2.1, 2.2, 2.3, 2.4 or 2.5, etc., and the proportion of pectinase can be 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1 .1, 1.2, 1.3, 1.4 or 1.5, etc., the portion of neutral protease can be 1.5, 1.6, 1.7, .8, 1.9, 2, 2.1, 2.2, 2.3, 2.4 or 2.5, etc., the portion of α-amylase can be 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4 or 1.5, etc., but are not limited to the values ​​listed above, and other values ​​not listed within the above numerical range are equally applicable.

[0023] Preferably, the mass of the enzyme is 0.1-2% of the mass of the plant raw material, such as 0.1%, 0.5%, 1%, 1.5% or 2%, etc., but is not limited to the above values, and other values ​​not listed in the above range are also applicable.

[0024] Preferably, the extraction temperature is 30-60°C and the time is 1-3h, wherein the temperature can be 30°C, 35°C, 40°C, 45°C, 50°C, 55°C or 60°C, etc., and the time can be 1h, 1.5h, 2h, 2.5h or 3h, etc., but are not limited to the values ​​listed above, and other values ​​not listed within the above numerical range are also applicable.

[0025] Preferably, the emulsifier comprises any one of Tween 80, sucrose fatty acid ester or monoglyceride, or a combination of at least two thereof.

[0026] In a second aspect, the present invention provides a method for preparing the functional feed additive for preventing and treating hepatoenteric cysts of shrimp as described above, the preparation method comprising the following steps:

[0027] The plant extract is mixed with essential oil, emulsifier and eugenol, and emulsified and homogenized to obtain the functional feed additive for preventing and treating hepatoenterozoa of shrimp.

[0028] In a third aspect, the present invention also provides the use of the functional feed additive for preventing and controlling hepatoenteric cytozoa of shrimp as described above in the preparation of shrimp feed.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The invention provides a functional feed additive for preventing and treating hepatoenteric cysts of shrimp. By compounding and synergizing essential oils, plant extracts and eugenol, the immunity of white shrimp can be effectively improved, the infection of hepatoenteric cysts in the cultivation process of white shrimp can be reduced, the survival rate and growth performance of white shrimp can be improved, and the hepatoenteric cysts can be inhibited and killed. DETAILED DESCRIPTION

[0031] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0032] In the following examples, citronella oil was purchased from Shanghai Jingchun Biotechnology Co., Ltd.;

[0033] Sea buckthorn oil was purchased from Heze Zhonghe Jianyuan Biotechnology Co., Ltd.;

[0034] Lemon oil was purchased from Guangzhou Jingjing Biotechnology Co., Ltd.;

[0035] Eugenol was purchased from Jiangxi Yisenyuan Plant Spice Co., Ltd.

[0036] Preparation Example 1

[0037] This preparation example provides a plant extract, and the preparation method is as follows:

[0038] The plant raw materials (Sophora flavescens, aloe vera or pomegranate peel) are crushed and mixed with enzymes (cellulase, pectinase, neutral protease and α-amylase, mass ratio of 2:1:2:1) and water and put into an ultrasonic extraction tank, the mass ratio of the plant raw material to water is 1:30, the mass of the enzyme is 1% of the mass of the plant raw material, the temperature of the ultrasonic extraction tank is 50 degrees, the extraction time is 2h (including 40min of ultrasonic treatment time), after the extraction is completed, the enzyme is inactivated (90℃ / 10min), then filtered through a filter, and cooled to obtain extracts (Sophora flavescens extract, aloe vera extract, pomegranate peel extract).

[0039] Preparation Example 2

[0040] This preparation example provides a plant extract, and the preparation method is as follows:

[0041] The plant raw materials (Sophora flavescens, aloe vera or pomegranate peel) are crushed and mixed with enzymes (cellulase, pectinase, neutral protease and α-amylase, mass ratio of 1.5:0.5:2.5:1.5) and water and put into an ultrasonic extraction tank, the mass ratio of plant raw materials to water is 1:25, the mass of the enzyme is 0.1% of the mass of the plant raw materials, the temperature of the ultrasonic extraction tank is 30 degrees, the extraction time is 3h (including 40min of ultrasonic treatment time), after the extraction is completed, the enzyme is inactivated (90℃ / 10min), then filtered through a filter, and cooled to obtain extracts (Sophora flavescens extract, aloe vera extract, pomegranate peel extract).

[0042] Preparation Example 3

[0043] This preparation example provides a plant extract, and the preparation method is as follows:

[0044] The plant raw materials (Sophora flavescens, aloe vera or pomegranate peel) are crushed and mixed with enzymes (cellulase, pectinase, neutral protease and α-amylase, mass ratio of 2.5:1.5:1.5:0.5) and water and put into an ultrasonic extraction tank, the mass ratio of the plant raw material to water is 1:35, the mass of the enzyme is 2% of the mass of the plant raw material, the temperature of the ultrasonic extraction tank is 60 degrees, the extraction time is 1h (including 40min of ultrasonic treatment time), after the extraction is completed, the enzyme is inactivated (90℃ / 10min), then filtered through a filter, and cooled to obtain extracts (Sophora flavescens extract, aloe vera extract, pomegranate peel extract).

[0045] Preparation Example 4

[0046] This preparation example provides a plant extract, and the preparation method is as follows:

[0047] The plant raw materials (Sophora flavescens, aloe vera or pomegranate peel) are crushed and mixed with water and put into an ultrasonic extraction tank. The mass ratio of the plant raw materials to water is 1:30. The temperature of the ultrasonic extraction tank is 50 degrees. The extraction time is 2 hours (of which the ultrasonic treatment time is 40 minutes). After the extraction is completed, the enzyme is inactivated (90°C / 10 minutes), and then filtered through a filter and cooled to obtain an extract (Sophora flavescens extract, aloe vera extract, pomegranate peel extract).

[0048] Example 1

[0049] This embodiment provides a functional feed additive for preventing and treating hepato-enteric cytozoa of shrimp, and the raw materials for preparation are as follows (by weight):

[0050] 15 parts of essential oils (citronella oil, sea buckthorn oil, lemon oil, mass ratio 2:3:4), 50 parts of plant extracts (Sophora flavescens extract, aloe extract, pomegranate peel extract prepared in Preparation Example 1, mass ratio 3:2:4), 2 parts of eugenol, and 5 parts of emulsifier (Tween 80).

[0051] The preparation method is as follows:

[0052] The plant extract is mixed with essential oil, emulsifier and eugenol, and emulsified and homogenized to obtain the functional feed additive for preventing and treating hepatoenterozoa of shrimp.

[0053] Example 2

[0054] This embodiment provides a functional feed additive for preventing and treating hepato-enteric cytozoa of shrimp, and the raw materials for preparation are as follows (by weight):

[0055] 5 parts of essential oils (citronella oil, sea buckthorn oil, lemon oil, mass ratio 1:2:3), 60 parts of plant extracts (Sophora flavescens extract, aloe extract, pomegranate peel extract prepared in Preparation Example 2, mass ratio 2:1:3), 0.5 parts of eugenol, and 10 parts of emulsifier (monoglyceride).

[0056] The preparation method is as follows:

[0057] The plant extract is mixed with essential oil, emulsifier and eugenol, and emulsified and homogenized to obtain the functional feed additive for preventing and treating hepatoenterozoa of shrimp.

[0058] Example 3

[0059] This embodiment provides a functional feed additive for preventing and treating hepato-enteric cytozoa of shrimp, and the raw materials for preparation are as follows (by weight):

[0060] 20 parts of essential oils (citronella oil, sea buckthorn oil, lemon oil, mass ratio 3:5:6), 30 parts of plant extracts (Sophora flavescens extract, aloe extract, pomegranate peel extract prepared in Preparation Example 3, mass ratio 4:3:5), 3 parts of eugenol, and 1 part of emulsifier (monoglyceride).

[0061] The preparation method is as follows:

[0062] The plant extract is mixed with essential oil, emulsifier and eugenol, and emulsified and homogenized to obtain the functional feed additive for preventing and treating hepatoenterozoa of shrimp.

[0063] Example 4

[0064] This embodiment provides a functional feed additive for preventing and treating hepatoenteric cysts of shrimp. The raw materials for preparation do not contain citronella oil, and the reduced part is allocated to sea buckthorn oil and lemon oil in proportion. The rest is the same as in Example 1.

[0065] Example 5

[0066] This embodiment provides a functional feed additive for preventing and treating hepatoenteric cysts of shrimp. The raw materials for preparation do not contain seabuckthorn oil, and the reduced part is allocated to citronella oil and lemon oil in proportion. Others are consistent with those in Example 1.

[0067] Example 6

[0068] This embodiment provides a functional feed additive for preventing and treating hepatoenteric cysts of shrimp. The raw materials for preparation do not contain lemon oil, and the reduced part is allocated to sea buckthorn oil and citronella oil in proportion. Others are consistent with those in Example 1.

[0069] Example 7

[0070] This example provides a functional feed additive for preventing and treating hepatoenteric cysts of shrimp. The raw materials for preparation do not contain Sophora flavescens extract, and the reduced portion is proportionally allocated to aloe extract and pomegranate peel extract (both from Preparation Example 1), and the rest are consistent with Example 1.

[0071] Example 8

[0072] This embodiment provides a functional feed additive for preventing and treating hepatoenteric cysts of shrimp. The preparation raw materials are consistent with Example 1 except that aloe extract is not included and the reduced portion is proportionally allocated to Sophora flavescens extract and pomegranate peel extract (both from Preparation Example 1).

[0073] Example 9

[0074] This example provides a functional feed additive for preventing and treating hepatoenteric cysts of shrimp. The raw materials for preparation are consistent with those of Example 1 except that the pomegranate peel extract is not included and the reduced portion is proportionally allocated to aloe extract and sophora flavescens extract (both from Preparation Example 1).

[0075] Example 10

[0076] This example provides a functional feed additive for preventing and treating hepatoenteric cysts of shrimp. The preparation raw materials are the same as those of Example 1 except that the plant extract provided in Preparation Example 1 is replaced by an equal amount of the plant extract provided in Preparation Example 4.

[0077] Embodiment 11

[0078] This embodiment provides a functional feed additive for preventing and treating hepatoenteric cysts of shrimp. The preparation raw materials are the same as those in Example 1, except that the plant extract provided in Preparation Example 1 is replaced by commercially available plant extracts (Sophora flavescens extract is purchased from Shaanxi Xinyanghe Biotechnology Co., Ltd., Aloe extract is purchased from Shanxi Fushengda Biotechnology Co., Ltd., and Pomegranate peel extract is purchased from Shaanxi Xinyanghe Biotechnology Co., Ltd.).

[0079] Comparative Example 1

[0080] This comparative example provides a functional feed additive for preventing and treating hepatoenteric cysts of shrimp. The raw materials for its preparation do not contain essential oils, and the reduced part is allocated to plant extracts and eugenol in proportion, and the rest is consistent with Example 1.

[0081] Comparative Example 2

[0082] This comparative example provides a functional feed additive for preventing and treating hepatoenteric cysts of shrimp. The raw materials for preparation do not contain plant extracts, and the reduced part is allocated to essential oils and eugenol in proportion, and the rest is consistent with Example 1.

[0083] Comparative Example 3

[0084] This comparative example provides a functional feed additive for preventing and treating hepatoenteric cysts of shrimp. The raw materials for preparation do not contain eugenol, and the reduced part is allocated to plant extracts and essential oils in proportion. The rest is consistent with Example 1.

[0085] Effect test:

[0086] The following tests selected healthy whiteleg shrimp juveniles that were robust, vigorous, and uniform in size, weighing 1.0-1.2 g and 4.0-4.3 cm in length as test materials.

[0087] The shrimp were tested for hepatocellular carcinoma infection, and the copy number of the parasite was 1×10 3 The formal test can start when the number of copies / μg is less than 100. The test is divided into 15 groups, each with 40 shrimps, and the test period is 28 days.

[0088] The functional feed additives for preventing and controlling hepatoenteric cysts of shrimp provided in the above Examples 1-11 and Comparative Examples 1-3 were tested. The control group was fed with a white shrimp formula (purchased from Guangdong Hengxing Feed Technology Co., Ltd.) infected with hepatoenteric cysts, and the white shrimp infected with hepatoenteric cysts in Examples 1-11 and Comparative Examples 1-3 were fed with a functional feed additive (50 mg / kg) added to the formula.

[0089] Growth indicators

[0090] After 28 days of feeding, the shrimp growth indicators include: survival rate SR (%), specific growth rate SGR (%, d-1), feed coefficient FCR (%). The calculation formula is as follows:

[0091] Survival rate SR (%) = number of shrimps remaining at the end of the experiment × 100 / number of shrimps released at the beginning of the experiment;

[0092] Specific growth rate SGR (%, d-1) = 100 × (ln final average weight - ln initial average weight) / experimental days;

[0093] Feed efficiency FE (%) = 100 × weight gain / (amount of feed fed - amount of residual feed).

[0094] The results are as follows:

[0095] Group Survival rate SR (%) Specific growth rate SGR (%) Feed efficiency FE (%) Example 1 45.00 1.76 0.13 Example 2 40.00 1.68 0.11 Example 3 42.50 1.70 0.12 Example 4 37.50 1.58 0.10 Example 5 35.00 1.55 0.09 Example 6 37.50 1.60 0.10 Example 7 35.00 1.53 0.09 Example 8 35.00 1.51 0.08 Example 9 32.50 1.48 0.07 Example 10 35.00 1,.62 0.10 Embodiment 11 32.50 1.59 0.09 Comparative Example 1 22.50 1.42 0.07 Comparative Example 2 20.00 1.36 0.06 Comparative Example 3 25.00 1.47 0.08 Control group 12.50 1.25 0.04

[0096] Nonspecific immunoenzyme activity assay

[0097] After the experiment, 3 shrimps were randomly selected from each group, and their hepatopancreas tissues were dissected and placed in 1.5 mL centrifuge tubes, and then quickly frozen in liquid nitrogen for determination. The preserved tissue samples were taken out of liquid nitrogen, 9 volumes (W / V) of physiological saline were added, and the samples were crushed with a tissue crusher, and then centrifuged at 5000r / min and 4°C for 10 minutes. The supernatant was diluted and then the enzyme activity and total protein were determined. The corresponding enzyme kits produced by Nanjing Jiancheng Technology Co., Ltd. were used to determine the nonspecific immune enzyme activities, including alkaline phosphatase (AKP), superoxide dismutase (SOD) and lysozyme (LZM).

[0098] The results are as follows:

[0099]

[0100]

[0101] The copy number of shrimp hepatocellular carcinoma

[0102] After the experiment, three shrimps were randomly selected from each group, and their hepatopancreas and intestines were dissected. The copy number of shrimp hepatopancreas and intestines was detected by qPCR. The results are as follows:

[0103] Group Hepatopancreas Intestinal Example 1 <![CDATA[1.146×10 2 ]]> 2.86 Example 2 <![CDATA[1.642×10 2 ]]> 3.21 Example 3 <![CDATA[1.564×10 2 ]]> 4.35 Example 4 <![CDATA[3.461×10 2 ]]> 23.14 Example 5 <![CDATA[2.673×10 2 ]]> 30.05 Example 6 <![CDATA[3.802×10 2 ]]> 26.73 Example 7 <![CDATA[5.631×10 2 ]]> 35.62 Example 8 <![CDATA[6.012×10 2 ]]> 37.09 Example 9 <![CDATA[6.694×10 2 ]]> 40.21 Example 10 <![CDATA[8.236×10 2 ]]> 33.25 Embodiment 11 <![CDATA[1.053×10 3 ]]> 42.38 Comparative Example 1 <![CDATA[3.562×10 3 ]]> 102.43 Comparative Example 2 <![CDATA[6.834×10 3 ]]> 189.76 Comparative Example 3 <![CDATA[2.047×10 3 ]]> 137.25 Control group <![CDATA[2.259×10 4 ]]> 362.7

[0104] From the above data, it can be found that the functional feed additive for preventing and treating hepatoenteric cysts of shrimp provided by the present invention can effectively improve the immunity of white shrimp, reduce the infection of hepatoenteric cysts during the cultivation of white shrimp, improve the survival rate and growth performance of white shrimp, inhibit and kill hepatoenteric cysts; Comparison of Examples 1-11 shows that the present invention can further improve the effect of the product by selecting a specific essential oil and a plant extract combination and adopting a specific extraction method compared with the prior art; Comparison of Example 1 and Comparative Examples 1-3 shows that the present invention can effectively improve the immunity of white shrimp, reduce the infection of hepatoenteric cysts during the cultivation of white shrimp, improve the survival rate and growth performance of white shrimp, and inhibit and kill hepatoenteric cysts by adopting a compound and synergistic effect of essential oil, plant extract and eugenol.

[0105] The applicant declares that the present invention uses the above-mentioned embodiments to illustrate the functional feed additive for preventing and treating hepatocellular carcinoma of shrimp and its preparation method and application, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0106] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0107] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A functional feed additive for preventing and treating hepatoenteric cysts of shrimp, characterized in that: The functional feed additive comprises, by weight, 5-20 parts of essential oil, 30-60 parts of plant extract, 0.5-3 parts of eugenol and 1-10 parts of emulsifier.

2. The functional feed additive for preventing and treating hepatoenteric cysts of shrimp according to claim 1, characterized in that: The essential oil includes any one of citronella oil, sea buckthorn oil, eucalyptus oil, garlic oil or lemon oil, or a combination of at least two of them, preferably a combination of citronella oil, sea buckthorn oil and lemon oil.

3. The functional feed additive for preventing and treating hepatoenteric cysts of shrimp according to claim 2, characterized in that: The mass ratio of the citronella oil, sea buckthorn oil and lemon oil is (1-3):(2-5):(3-6).

4. The functional feed additive for preventing and treating hepatoenteric cysts of shrimp according to any one of claims 1 to 3, characterized in that: The plant extract comprises any one of a Sophora flavescens extract, an Aloe vera extract, and a Pomegranate peel extract, or a combination of at least two of them, preferably a combination of a Sophora flavescens extract, an Aloe vera extract, and a Pomegranate peel extract.

5. The functional feed additive for preventing and treating hepatoenteric cysts of shrimp according to claim 4, characterized in that: The mass ratio of the Sophora flavescens extract, the Aloe vera extract and the Pomegranate peel extract is (2-4):(1-3):(3-5).

6. The functional feed additive for preventing and treating hepato-enteric cytozoa of shrimp according to any one of claims 1 to 5, characterized in that: The plant extract is obtained by extracting by a method comprising the following steps: The plant raw material is crushed, then mixed with enzyme and water for ultrasonic extraction, and filtered to obtain the plant extract.

7. The functional feed additive for preventing and treating hepatoenteric cysts of shrimp according to claim 6, characterized in that: The mass ratio of the plant raw material to water is 1:(25-35); Preferably, the enzymes include cellulase, pectinase, neutral protease and α-amylase in a mass ratio of (1.5-2.5):(0.5-1.5):(1.5-2.5):(0.5-1.5); Preferably, the mass of the enzyme is 0.1-2% of the mass of the plant raw material; Preferably, the extraction temperature is 30-60°C and the extraction time is 1-3h.

8. The functional feed additive for preventing and treating hepatoenteric cysts of shrimp according to any one of claims 1 to 7, characterized in that: The emulsifier includes any one of Tween 80, sucrose fatty acid ester or monoglyceride or a combination of at least two thereof.

9. A method for preparing a functional feed additive for preventing and treating hepatoenteric cytozoa of shrimp according to any one of claims 1 to 8, characterized in that: The preparation method comprises the following steps: The plant extract is mixed with essential oil, emulsifier and eugenol, and emulsified and homogenized to obtain the functional feed additive for preventing and treating hepatoenterozoa of shrimp.

10. Use of the functional feed additive for preventing and controlling hepatoenteric cytozoa of shrimp according to any one of claims 1 to 8 in the preparation of shrimp feed.

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