A recombinant Corynebacterium glutamicum with high 1,3-propanediol production and its construction method and use
By constructing recombinant Corynebacterium glutamicum, overexpressing key enzyme genes and enhancing the expression of enzymes related to the homoserine synthesis pathway, the existing microbial fermentation method has solved the problem of high cost and unstable yield in the production of 1,3-propylene glycol, and achieved high yield and low cost fermentation production results.
CN119752747APending Publication Date: 2025-04-04SHANGHAI RECOM BIOTECHNOLOGY CO LTD
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Patent Information
- Application Number
- CN202411782015.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-05
- Publication Date
- 2025-04-04
AI Technical Summary
Technical Problem
The existing microbial fermentation method has high cost, unstable yield and the need for exogenous addition of vitamin B12 when producing 1,3-propylene glycol.
Method used
By constructing recombinant Corynebacterium glutamicum, overexpressing the deaminase gene AAD, decarboxylase gene KDC, and aldehyde reductase gene yqhD, and enhancing the expression of enzyme genes related to the homoserine synthesis pathway, optimizing the fermentation conditions to increase the yield of 1,3-propylene glycol.
Benefits of technology
The yield of 1,3-propylene glycol in shake flask fermentation reached 6.7 g/L, and the yield of 54.3 g/L in fermentation tank was achieved, reducing the fermentation cost and improving production efficiency.
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Abstract
The present invention relates to a recombinant Corynebacterium glutamicum with high yield of 1,3-propylene glycol, wherein the recombinant Corynebacterium glutamicum can overexpress the following genes: deaminase gene AAD, decarboxylase gene KDC, and aldehyde reductase gene yqhD. The present invention enables Corynebacterium glutamicum to overexpress AAD gene, KDC gene and yqhD gene, and enhances the expression amount of homoserine synthesis pathway related enzyme genes, so that in the process of fermentation and production of 1,3-propylene glycol, the yield in the shake flask stage is as high as 6.7g / L, the yield in the fermentation tank is as high as 54.3g / L, the fermentation cost is low and the yield is high, the benefit of producing 1,3-propylene glycol is maximized, and it is suitable for practical promotion and application.
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