InDel molecular marker for identifying resistance of tea tree anthracnose as well as primer and application of InDel molecular marker

By developing InDel molecular markers located on the Chr10 chromosome in tea trees, the problem of difficulty in identifying anthrax varieties in tea trees is solved in the prior art, and rapid and accurate resistance identification is achieved, shortening breeding cycles and reducing costs.

CN119753211APending Publication Date: 2025-04-04SOUTH CHINA AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202411940043.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-26
Publication Date
2025-04-04

AI Technical Summary

Technical Problem

The prior art is difficult to effectively identify and breed varieties that resist tea tree anthrax, and lacks efficient molecular markers for tea tree anthrax resistance.

Method used

An InDel molecular marker was developed, located at the 123508930bp position of the chromosome of Chr10 in tea tree. The specific InDel marker was identified through genome resequencing and RNA-seq information, and used to identify the anthrax resistance and sensory varieties of tea tree, and designed corresponding primers for PCR amplification and kit development.

Benefits of technology

It has achieved rapid, accurate and efficient identification of anthrax-resistant varieties in tea trees, shortened breeding cycles, reduced breeding costs, and provided an effective molecular marking tool for tea trees to resist anthrax breeding.

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Abstract

The invention discloses an InDel molecular marker for tea tree anthracnose resistance identification and primers and application of the InDel molecular marker. Based on re-sequencing of genomes of tea tree varieties with different resistances, a specific InDel marker is identified and obtained in a mutation site on a plant and pathogen interaction pathway gene, and the genotype at the position of the marker can be used for identifying anthracnose-resistant and susceptible varieties of tea trees. The InDel marker primer designed for the site can identify the tea tree in the seedling stage, the breeding period is shortened, the anthracnose-resistant variety of the tea tree can be rapidly, accurately and efficiently identified, and the InDel marker primer has wide applicability in tea tree breeding. Compared with a traditional breeding phenotype identification method, the InDel molecular marker primer is accurate and reliable in identification, breeding target progenies can be screened in advance, the breeding period is shortened, the breeding cost is reduced, and rich molecular markers are provided for genetic and genome research of tea trees so as to promote further research on anthracnose-resistant breeding plans of the tea trees.
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