A pesticide composition for controlling thrips of grape

Abstract

This invention belongs to the field of pesticide technology, specifically relating to a pesticide composition for controlling grape thrips. The pesticide composition for controlling grape thrips is an insecticidal composition, wherein the active ingredient of the insecticidal composition is a compound of methoxypiperidine ethyl ester and cyclophosphamide or rotenone; wherein the mass ratio of methoxypiperidine ethyl ester to cyclophosphamide is 1-8:12-1; and the mass ratio of methoxypiperidine ethyl ester to rotenone is 1-20:20-1. The combination of methoxypiperidine ethyl ester and cyclophosphamide or rotenone in this invention, through indoor activity tests, shows that the compound combination exhibits a synergistic effect against grape thrips, improving the control efficacy against grape thrips. Compared with single agents, it can reduce the risk of resistance, delay the emergence and development of resistance in thrips, and provide support for the management of thrips resistance and ensuring safe grape production.

Description

Technical Field

[0001] This invention belongs to the field of pesticide control technology, specifically relating to a pesticide composition for controlling grape thrips. Background Technology

[0002] Grapes are a common fruit in the market, highly valued for their diverse varieties, high nutritional and economic value. In recent years, with the expansion of grape cultivation, pest and disease problems have become increasingly prominent. The tobacco thrips (Thripstabaci lindeman) is one of the most significant pests affecting grapes. Belonging to the order Thysanoptera and family Thripidae, it feeds on the leaves, flowers, stems, and fruits of the plant using its rasping-sucking mouthparts. In grape-growing areas, tobacco thrips suck the sap from grapes, especially unripe fruit. The surface of the fruit may develop symptoms such as dents and black spots, and sometimes even perforation, affecting fruit yield and quality. Furthermore, tobacco thrips can transmit viruses, such as the common tobacco stripe virus (TSV), iris yellow spot virus (IYSV), and tomato spotted wilt virus (TSWV), further damaging grapes.

[0003] Currently, pesticides are the primary method of controlling thrips in grape production. However, because tobacco thrips widely infest more than 150 plant species, including onions, cotton, grapes, and lilies, they face significant selection pressure from various pesticides, leading to rapid development of resistance. Existing research has confirmed that tobacco thrips have developed resistance to several existing pesticides, including pyrethroids, carbamates, organophosphates, and spinosad. As resistance accumulates year by year, the efficacy of pesticides will gradually decrease, and they may even become ineffective. Therefore, evaluating and screening new pesticides is of great significance for managing tobacco thrips resistance and ensuring safe grape production.

[0004] Spiropidion (CAS: 1229023-00-0) is a spirocyclic ketoacid insecticide developed by Syngenta, and is the fourth spirocyclic ketoacid insecticide after Bayer's spirodiclofen, spirodiclofen, and spirotetramat. Spiropidion is a lipid biosynthesis inhibitor, primarily working by inhibiting the activity of ACCase during lipid synthesis in pests, thus disrupting lipid synthesis and blocking normal energy metabolism, ultimately leading to pest death. Furthermore, spirropidion not only has a unique chemical structure and novel mode of action, but also possesses bidirectional, systemic translocation properties, acting on both larval stages and disrupting adult oviposition. This reduces the initial insect population while inhibiting the hatching of eggs already laid in plant tissues or on the surface, resulting in a potent insecticidal effect. It can also be used as a resistance management tool. Although spirropidion has these advantages, as a single insecticidal active ingredient, its action sites are limited, and long-term use as a single agent carries a high risk of resistance development. Summary of the Invention

[0005] The purpose of this invention is to provide a pesticide composition for controlling grape thrips, which combines methoxypiperidine ethyl ester with existing insecticides through screening and compounding, exhibiting a synergistic effect, thereby improving the control efficacy against grape thrips and reducing the risk of resistance to single-agent pesticides.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] A pesticide composition for controlling grape thrips, which is an insecticidal composition, wherein the active ingredient of the insecticidal composition is a compound of methoxypiperidine ethyl ester and cyclophosphamide or rotenone; wherein the mass ratio of methoxypiperidine ethyl ester to cyclophosphamide is 1-8:12-1; and the mass ratio of methoxypiperidine ethyl ester to rotenone is 1-20:20-1.

[0008] An insecticide comprising the aforementioned insecticidal composition.

[0009] Preferably, the insecticidal composition accounts for 0.5-75% of the total mass of the insecticide, with the remainder being auxiliary ingredients permitted to be added to the pesticide.

[0010] Preferably, the insecticidal composition accounts for 5-45% of the total mass of the insecticide, with the remainder being auxiliary ingredients permitted to be added to the pesticide.

[0011] Compared with the prior art, the present invention has the following beneficial effects:

[0012] This invention relates to a combination of methoxypiperidine ethyl ester and epoxim or rotenone. Indoor activity tests revealed that within a compound mass ratio range of 1-8:12-1, the combination of methoxypiperidine ethyl ester and epoxim showed a synergistic effect against *Tobacco thrips* in grapes; within a compound mass ratio range of 1-20:20-1, the combination of methoxypiperidine ethyl ester and rotenone also showed a synergistic effect. This indicates that the combination of methoxypiperidine ethyl ester with epoxim or rotenone can improve the control efficacy against *Tobacco thrips* in grapes. Compared with single agents, it can reduce the risk of resistance, delay the emergence and development of resistance in *Tobacco thrips*, and provide support for the management of *Tobacco thrips* resistance and ensure safe grape production. Detailed Implementation

[0013] The following is a clear and complete description of the technical solution of this invention patent. Obviously, the described embodiments are some, but not all, of the embodiments of this invention.

[0014] Example: Indoor toxicity test

[0015] 1. Test reagents

[0016] 95% methoxypiperazine technical grade, 95% cyclooxyfenozide technical grade, 95% rotenone technical grade

[0017] The original drug was dissolved in dimethyl sulfoxide and then diluted with distilled water containing 0.01% Triton X-100 to prepare a high-concentration single-dose stock solution. Multiple formulations were prepared, and each single agent and each formulation mixture was diluted with distilled water containing 0.01% Triton X-100 in equal proportions to form 5 mass concentration gradients. Each mass concentration solution was 50 mL and prepared for use.

[0018] 2. Test pests

[0019] The tobacco thrips were collected from the grape planting base of the Guangxi Academy of Agricultural Sciences. They were fed with fresh, clean, young grape leaves and continuously reared for four generations in an artificial climate chamber with a temperature of 25±1℃, a photoperiod of 16L / 8D, and a relative humidity of 70±5%. Second-instar nymphs of the same age were selected for toxicity testing.

[0020] 3. Test Methods

[0021] Take fresh, clean, young grape leaves, and cut leaf discs with a diameter of 2 cm. Immerse the leaf discs in the pesticide solution for 10 seconds, then remove and air dry. Take plastic centrifuge tubes and poke several small holes in the bottom and cap (the hole diameter should be large enough to prevent the test pests from escaping). Place the leaf discs into the plastic centrifuge tubes, three leaf discs per tube. Inoculate 15 test pests into each centrifuge tube, cap the tube, and observe under the original conditions. Set up four replicates for each concentration of pesticide solution, with each tube constituting one replicate. Include a treatment containing 0.01% Triton X-100 distilled water as a blank control.

[0022] After 48 hours, the mortality of the test insects was investigated (insects that could not move when lightly touched with a brush tip were considered dead), and the corrected mortality rate for each treatment was calculated. Regression analysis was performed on the logarithmic values ​​of the pesticide concentration and the corrected mortality rate probability values ​​for each treatment using DPS software to calculate the LC50 of the pesticide for each treatment. 50 .

[0023]

[0024] In the above formula: P -- mortality rate, in %; K -- number of dead insects; N -- total number of insects treated.

[0025]

[0026] In the above formula: P1 -- corrected mortality rate, in %; P t --Treatment mortality rate, in %; P0--Control mortality rate, in %.

[0027] 4. Pharmaceutical Evaluation

[0028] The co-toxicity coefficient (CTC value) of the mixture was calculated according to Sun Yunpei's method, and the combined effects were classified according to the following criteria: a CTC value ≥ 120 indicates a synergistic effect; a CTC value ≤ 80 indicates an antagonistic effect; and a CTC value < 80 < 120 indicates an additive effect. The experimental results are shown in Table 1-2.

[0029] Table 1. Combined Effect of Methoxypiperidine Ethyl Acetate and Epioxocetirizine on Grape Thrips

[0030]

[0031] Table 2. Combined Effect of Ethyl Methoxypiperidine and Rotenone on Thrips from Grapefruit

[0032]

[0033] The experimental results in Tables 1 and 2 show that, within the mass ratio range of 1-8:12-1, the combination of methoxypiperidine ethyl ester and epoxim showed a synergistic effect on grape thrips; and within the mass ratio range of 1-20:20-1, the combination of methoxypiperidine ethyl ester and rotenone showed a synergistic effect on grape thrips.

[0034] In summary, the combination of methoxypiperidine ethyl ester with cyclooxygenated chlorpyrifos or rotenone in this invention can improve the control effect against grape thrips. Compared with single agents, it can reduce the risk of resistance, delay the emergence and development of resistance in thrips, and provide support for the management of thrips resistance and the safe production of grapes.

Claims

1. A pesticide composition for controlling thrips of grape, which is an insecticidal composition, characterized by, The active ingredient of the insecticidal composition is a compound of methoxypiperidine ethyl ester and epoxim; wherein... The mass ratio of methoxypiperidine ethyl ester to epoxim is 1-8:12-1.

2. An insecticide, characterized in that, The insecticide includes the insecticidal composition of claim 1.

3. The insecticide of claim 2, wherein, The insecticidal composition comprises 0.5-75% of the total mass of the insecticide, with the remainder being auxiliary ingredients permitted to be added to the pesticide.

4. The insecticide of claim 2, wherein, The insecticidal composition accounts for 5-45% of the total mass of the insecticide, with the remainder being auxiliary ingredients permitted to be added to the pesticide.

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