Lip care sunscreen microcapsules and method of preparation and use in the preparation of a sunscreen and rejuvenating lip balm

Abstract

This invention discloses a lip care sunscreen microcapsule and its preparation method, as well as its application in the preparation of a sunscreen and revitalizing lip balm. The microcapsule comprises squalene, astaxanthin, pyrrolidone, candelilla wax, shea butter, lauryl laurate, zein, hydrolyzed corn starch octenyl succinate, silica, and benzyl ether myristate. This invention utilizes synthetic biology techniques to screen for a combination of bioactive substances—squalene / astaxanthin / pyrrolidone—with excellent anti-allergic properties. These substances can regulate the skin's immune response, reduce inflammation, and enhance the skin barrier function, thereby effectively reducing allergic reactions of sensitive skin to ultraviolet radiation.

Description

Technical Field

[0001] This invention belongs to the field of microcapsule application technology, specifically relating to a lip care sunscreen microcapsule and its preparation method, as well as its application in the preparation of sunscreen and revitalizing lip balm. Background Technology

[0002] In the field of lip care, there are currently various types of lip products on the market; among them, lip care products mainly focus on moisturizing and color enhancement. Common lip balms typically contain ingredients such as petrolatum, lanolin, and natural oils (such as jojoba oil and coconut oil). These ingredients form a protective film on the surface of the lips, reducing moisture evaporation and thus keeping the lips moisturized. For example, some well-known brands of lip balms can relieve dryness and peeling of the lips in a short time. As for lip products with color functions, such as lipsticks and lip glosses, in addition to adding pigments, they also contain certain moisturizing ingredients, providing a certain degree of repair while giving the lips color.

[0003] In terms of sun protection, there are many types of facial sunscreens. Their sun protection principle mainly involves adding organic sunscreens (such as para-aminobenzoic acid and its derivatives, cinnamic acid esters, etc.) and inorganic sunscreens (such as titanium dioxide, zinc oxide) to absorb or reflect ultraviolet rays. These sunscreens can effectively resist UVA and UVB rays, protecting facial skin from photoaging, sunburn, and other damage. However, these facial sunscreens are not specifically designed for the lips. Facial sunscreens are usually quite thick, causing noticeable discomfort when applied to the lips, affecting their feel and appearance. While some lipsticks or lip glosses containing sunscreen ingredients offer some sun protection and color benefits, their SPF is often low, failing to provide sufficient UV protection, and reapplication can easily ruin the original lip makeup. Furthermore, these products lack long-lasting sun protection, failing to effectively protect the lips from UV damage during prolonged outdoor activities. Color is also a factor; red is a universally flattering color.

[0004] Prodigiosin is a tripyrrole ring structure with a maximum absorption wavelength of 530 nm, synthesized by bacteria such as *Serratia marcescens*. Studies by Suryawanshi et al. have shown that prodigiosin, due to its photosensitive properties, can be used as an additive in sunscreen products to reduce UV damage to the skin. However, when used alone as a sunscreen agent, prodigiosin does not produce a meaningful SPF value, but it can increase the SPF value of sunscreen products or simple plant extracts.

[0005] Astaxanthin is a natural pigment with strong antioxidant capabilities. It is mainly found in marine organisms such as shrimp and crabs. Astaxanthin can absorb ultraviolet (UV) radiation, especially long-wave UVA, reducing its harmful effects on the skin. Simultaneously, its antioxidant properties can eliminate free radicals generated by UV radiation, preventing damage to skin cells and thus providing sun protection.

[0006] Pyrroloquinoline quinone (PQQ) exhibits maximum absorption at a wavelength of 250 nm, effectively preventing photoaging caused by ultraviolet radiation. Currently, existing active ingredient encapsulation technologies are mostly in the form of oil-in-water emulsions, which are not easily added to lip products. Summary of the Invention

[0007] The purpose of this section is to outline some aspects of embodiments of the present invention and to briefly describe some preferred embodiments. Simplifications or omissions may be made in this section, as well as in the abstract and title of this application, to avoid obscuring the purpose of these documents; however, such simplifications or omissions should not be construed as limiting the scope of the invention.

[0008] In view of the problems existing in the above and / or prior art, the present invention is proposed.

[0009] Therefore, the purpose of this invention is to overcome the shortcomings of the prior art and provide a multi-compartment microcapsule for lip care and sun protection.

[0010] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a multi-compartment microcapsule for lip care and sun protection, comprising: styracin, astaxanthin, pyrroloquinoline quinone, candelilla wax, shea butter, lauryl laurate, zein, hydrolyzed corn starch octenyl succinate, silica, and benzyl ether myristate.

[0011] Based on the percentage by weight of the raw materials, the pyrrolizin is 0.1%–5%, the astaxanthin is 0.1%–5%, the pyrroloquinoline quinone is 0.1%–5%, the candelilla wax is 5%–20%, the shea butter is 5%–20%, the lauryl laurate is 5%–10%, the zein is 1%–5%, the hydrolyzed corn starch octenyl succinate is 20%–40%, the silica is 10%–20%, and the benzyl ether myristate is 5%–10%.

[0012] As a preferred embodiment of the multi-chamber microcapsule for lip care and sun protection described in this invention, wherein the multi-chamber microcapsule achieves enzyme-controlled release.

[0013] Another objective of this invention is to overcome the shortcomings of the prior art and provide a method for preparing multi-compartment microcapsules for lip care and sun protection, comprising,

[0014] Heat the lysimachia christinae, astaxanthin, pyrroloquinoline quinone, candelilla wax, and shea butter until they are completely miscible to form phase A.

[0015] Lauryl lauryl ester, benzyl ether myristate, and water were heated until completely miscible to form phase B.

[0016] Phase A was slowly added to phase B at 80°C and stirred until homogeneous. Then, the mixture was homogenized under high pressure at 800 bar to obtain phase C.

[0017] Zeat protein and hydrolyzed corn starch octenyl succinate were dissolved in deionized water and hydrated overnight to obtain a transparent liquid. Then, the liquid was heated and stirred in a water bath at 90°C, silica was added, and the mixture was stirred until homogeneous to obtain phase D.

[0018] Mix phase C and phase D, stir evenly, and put into a micro high-pressure atomizing pump. Use an open container to hold liquid nitrogen, then aim the nozzle of the atomizing pump at the liquid nitrogen and start the pump to spray.

[0019] After spraying, the open container was placed in a freeze dryer and vacuum dried to obtain multi-chamber microcapsules.

[0020] In a preferred embodiment of the preparation method described in this invention, the heating temperature during the preparation of phase A is 80°C.

[0021] In a preferred embodiment of the preparation method described in this invention, the heating temperature during the preparation of phase B is 80°C.

[0022] In a preferred embodiment of the preparation method described in this invention, the high-pressure homogenization process during the preparation of the C phase is repeated 3 to 5 times.

[0023] In a preferred embodiment of the preparation method described in this invention, the nozzle used for spraying is 0.2 mm.

[0024] Another objective of this invention is to overcome the shortcomings of the prior art and provide an application of multi-compartment microcapsules for lip care and sun protection in the preparation of sun-protective and revitalizing lip balm.

[0025] As a preferred embodiment of the application described in this invention, the sunscreen and revitalizing lip balm comprises the following components by mass fraction:

[0026] Lip care sunscreen multi-compartment microcapsules 10%–30%, microcrystalline wax 10%–30%, mica 10%–15%, burdock seed oil 2%–10%, isononyl isononanoate 2%–10%, jojoba seed oil 2%–10%, polyglycerol-3-diisostearate 2%–5%, hydroxypropyl tetrahydropyranotriol 0.1%–2%, sodium hyaluronate 0.1%–2%, glucomannan 0.1%–2%, water to 100%.

[0027] In a preferred embodiment of the application described in this invention, the method for preparing the sunscreen and revitalizing lip balm includes,

[0028] Microcrystalline wax, mica, burdock seed oil, isononyl isononanoate, jojoba seed oil, and polyglycerol-3-diisostearate were mixed, stirred, dissolved, and heated until homogeneous to obtain the first phase.

[0029] Hydroxypropyltetrahydropyranotriol, sodium hyaluronate, glucomannan and water were mixed, stirred and dissolved, and heated until uniformly dissolved to obtain the second phase.

[0030] Mix the first and second phases evenly, add the lip care sunscreen multi-chamber microcapsules, keep warm and stir evenly, cool the paste to 62°C, pour it into a mold or tube to form and discharge, and obtain the sunscreen and revitalizing lip balm.

[0031] Beneficial effects of this invention:

[0032] (1) This invention uses synthetic biology technology to screen out a combination of bioactive substances with excellent anti-allergic properties, namely styracin / astaxanthin / pyrroloquinoline quinone. These substances can regulate the skin's immune response, reduce inflammation, and enhance the skin barrier function, thereby effectively reducing the allergic reaction of sensitive skin to ultraviolet rays.

[0033] (2) The present invention uses nanotechnology and low temperature spray freezing technology to prepare multi-chamber microcapsules, which can realize enzyme-controlled release.

[0034] (3) The present invention prepares a sunscreen and revitalizing lip balm that can gradually release functional components and has anti-aging, sun protection and self-coloring functions. Attached Figure Description

[0035] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Wherein:

[0036] Figure 1 This is a cryo-transmission electron microscope (CTEM) structural diagram of the sample in Example 1 of the present invention.

[0037] Figure 2 This is a graph showing the release time of the active ingredient in Example 2 of the present invention.

[0038] Figure 3 This is a diagram showing the anti-aging test results of the sample in Example 4 of the present invention.

[0039] Figure 4 This is a diagram showing the sun protection test results of the sample in Example 4 of the present invention. Detailed Implementation

[0040] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the examples in the specification.

[0041] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0042] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that is mutually exclusive with other embodiments.

[0043] The raw materials used in the embodiments of this invention are all commercially available products.

[0044] Example 1

[0045] Microcapsules were prepared according to the raw material ratios in Table 1:

[0046] (1) Heat pyrrolizin, astaxanthin, pyrroloquinoline quinone, candelilla wax, and shea butter at 80°C until they are completely miscible to form phase A;

[0047] (2) Heat lauryl laurate, PPG-3 benzyl ether myristate and water at 80°C until they are completely miscible to form phase B;

[0048] (3) At 80°C, phase A is slowly added to phase B and stirred until homogeneous. Then, the mixture is homogenized under high pressure for 5 times at 800 bar to obtain phase C.

[0049] (4) Corn glycerin and hydrolyzed corn starch octenyl succinate were dissolved in deionized water and hydrated overnight to obtain a transparent liquid. Then, the liquid was heated and stirred in a water bath at 90°C, silica was added, and the mixture was stirred until homogeneous to obtain phase D.

[0050] (5) Mix phase C and phase D, stir evenly, and put into a micro high-pressure atomizing pump. Use an open container to hold liquid nitrogen, then aim the atomizing pump nozzle at the liquid nitrogen, start the pump, and use a 0.2mm nozzle.

[0051] After spraying, the open container is placed in a freeze dryer and vacuum dried to obtain powder.

[0052] Table 1 Raw Material Proportions (Unit: g)

[0053]

[0054] The resulting microcapsules will disperse in the oil solution without precipitation and can form a homogeneous liquid at 80°C. If the composition is not in the correct proportion, microcapsules cannot be formed and cannot be uniformly dispersed in the oil solution, resulting in powder precipitation.

[0055] The structure of sample 1 was characterized using scanning electron microscopy, see [link to sample 1]. Figure 1 .

[0056] from Figure 1 It can be seen that the microcapsules prepared from sample 1 are microspheres with a multi-chamber structure.

[0057] Example 2

[0058] Weigh an appropriate amount of sample 1 from Example 1, add different amounts of α-amylase to make small tablets with a diameter of 10 mm, each tablet containing approximately 20 mg of styraxin.

[0059] A drug dissolution apparatus was used, employing the slurry method, with 500 mL of pH 7.4 phosphate buffer (10% methanol) as the dissolution medium, at a temperature of (37±0.5)℃ and a rotation speed of 100 r / min.

[0060] Take a 5.0 mL sample every hour, and simultaneously add 5.0 mL of dissolution medium at the same temperature to the release system. After filtering with a 0.22 μm microporous membrane, dilute the filtrate appropriately with 10% methanol solution, and determine the content of styraxone by high performance liquid chromatography.

[0061] The cumulative release rate of the active ingredient is calculated using the following formula:

[0062]

[0063] In the formula, W0 is the active ingredient content (mg), Vt is the medium volume, Vs is the sampling volume, ci is the concentration of the active ingredient in the released liquid at time t for the i-th measurement, and ∑ci-1 is the sum of the active ingredient concentrations measured in the previous i-1 measurements.

[0064] The effect of α-amylase on the controlled release of active substances from microcapsules was investigated. The cumulative release rate of active substances from microcapsules versus time was tested for different enzyme concentrations. The results are as follows: Figure 2 As shown;

[0065] As can be seen from the figure, the higher the enzyme content, the faster the active ingredient is released. When no enzyme is present, the microcapsules only release a small amount due to temperature.

[0066] Example 3

[0067] (1) Sunscreen and revitalizing lip balm formula (all raw materials used are conventional cosmetic raw materials, purchased from cosmetic raw material companies such as Shanghai Puen Biochemical Co., Ltd. and Shanghai Kein Co., Ltd.):

[0068] Lip care sun protection multi-compartment microcapsules 20% (Sample 1 in Example 1);

[0069] Microcrystalline wax 25%;

[0070] 10% mica;

[0071] Burr hard walnut seed oil 8%;

[0072] Isononyl isononate 8%;

[0073] Jojoba seed oil 8%;

[0074] 4% polyglycerol-3-diisostearate;

[0075] Hydroxypropyltetrahydropyranotriol 1%;

[0076] Sodium hyaluronate 0.1%;

[0077] 1% glucomannan;

[0078] Replenish water to 100%.

[0079] (2) Preparation method of sunscreen and revitalizing lip balm: Mix microcrystalline wax, mica, burdock seed oil, isononyl isononanoate, jojoba seed oil and polyglycerol-3-diisostearate, stir and dissolve, heat and stir to 82℃ to disperse / dissolve evenly;

[0080] Hydroxypropyl tetrahydropyranotriol, sodium hyaluronate, glucomannan and water are mixed, stirred and dissolved, heated and stirred to 82°C until uniformly dispersed / dissolved, and then the two are mixed and stirred evenly.

[0081] Add multi-chamber microcapsules for lip care and sun protection, keep warm and stir to disperse evenly, cool the paste to 62°C, pour it into a mold or tube to form and discharge, and obtain the sun-protective and revitalizing lip balm.

[0082] Comparative Example 1

[0083] (1) Ingredients for sunscreen and revitalizing lip balm:

[0084] Lip care sunscreen multi-compartment microcapsules 20% (Sample 2 in Example 1, without active ingredients);

[0085] Microcrystalline wax 25%;

[0086] 10% mica;

[0087] Burr hard walnut seed oil 8%;

[0088] Isononyl isononate 8%;

[0089] Jojoba seed oil 8%;

[0090] 4% polyglycerol-3-diisostearate;

[0091] Hydroxypropyltetrahydropyranotriol 1%;

[0092] Sodium hyaluronate 0.1%;

[0093] 1% glucomannan;

[0094] Replenish water to 100%.

[0095] (2) Preparation method: Microcrystalline wax, mica, burdock seed oil, isononyl isononanoate, jojoba seed oil and polyglycerol-3-diisostearate are mixed, stirred and dissolved, and heated and stirred to 82°C to disperse / dissolve evenly;

[0096] Hydroxypropyl tetrahydropyranotriol, sodium hyaluronate, glucomannan and water are mixed, stirred and dissolved, and heated to 82°C to disperse / dissolve evenly. The two are mixed and stirred evenly, and then lip care sunscreen multi-chamber microcapsules are added. The mixture is kept warm and stirred evenly. The paste is cooled to 62°C and poured into a mold or tube to form and discharge, thus obtaining the sunscreen and revitalizing lip balm.

[0097] Comparative Example 2

[0098] (1) Sunscreen and revitalizing lip balm:

[0099] Lip care sunscreen multi-compartment microcapsules 20% (sample 8 in Example 1);

[0100] Microcrystalline wax 25%;

[0101] 10% mica;

[0102] Burr hard walnut seed oil 8%;

[0103] Isononyl isononate 8%;

[0104] Jojoba seed oil 8%;

[0105] 4% polyglycerol-3-diisostearate;

[0106] Hydroxypropyltetrahydropyranotriol 1%;

[0107] Sodium hyaluronate 0.1%;

[0108] 1% glucomannan;

[0109] Replenish water to 100%.

[0110] (2) Preparation method: Microcrystalline wax, mica, burdock seed oil, isononyl isononanoate, jojoba seed oil and polyglycerol-3-diisostearate are mixed, stirred and dissolved, and heated and stirred to 82°C to disperse / dissolve evenly;

[0111] Hydroxypropyl tetrahydropyranotriol, sodium hyaluronate, glucomannan and water are mixed, stirred and dissolved, and heated to 82°C for uniform dispersion / dissolution; the two are mixed and stirred evenly, lip care sunscreen multi-chamber microcapsules are added, and the mixture is kept warm and stirred evenly. The paste is cooled to 62°C, poured into a mold or tube to form and discharge, thus obtaining the sunscreen and revitalizing lip balm.

[0112] Comparative Example 3

[0113] (1) Sunscreen and revitalizing lip balm:

[0114] Lip care sunscreen multi-compartment microcapsules 20% (sample 9 in Example 1);

[0115] Microcrystalline wax 25%;

[0116] 10% mica;

[0117] Burr hard walnut seed oil 8%;

[0118] Isononyl isononate 8%;

[0119] Jojoba seed oil 8%;

[0120] 4% polyglycerol-3-diisostearate;

[0121] Hydroxypropyltetrahydropyranotriol 1%;

[0122] Sodium hyaluronate 0.1%;

[0123] 1% glucomannan;

[0124] Replenish water to 100%.

[0125] (2) Preparation method: Microcrystalline wax, mica, burdock seed oil, isononyl isononanoate, jojoba seed oil and polyglycerol-3-diisostearate are mixed, stirred and dissolved, and heated and stirred to 82°C to disperse / dissolve evenly;

[0126] Hydroxypropyl tetrahydropyranotriol, sodium hyaluronate, glucomannan and water are mixed, stirred and dissolved, and heated and stirred to 82°C until uniformly dispersed / dissolved;

[0127] Mix the two ingredients thoroughly, add the lip care sunscreen multi-chamber microcapsules, keep warm and stir to disperse evenly, cool the paste to 62°C, pour it into a mold or tube to form and discharge, and obtain the sunscreen revitalizing lip balm.

[0128] Comparative Example 4

[0129] (1) Sunscreen and revitalizing lip balm:

[0130] Lip care sunscreen multi-compartment microcapsules 20% (sample 10 in Example 1);

[0131] Microcrystalline wax 25%;

[0132] 10% mica;

[0133] Burr hard walnut seed oil 8%;

[0134] Isononyl isononate 8%;

[0135] Jojoba seed oil 8%;

[0136] 4% polyglycerol-3-diisostearate;

[0137] Hydroxypropyltetrahydropyranotriol 1%;

[0138] Sodium hyaluronate 0.1%;

[0139] 1% glucomannan;

[0140] Replenish water to 100%.

[0141] (2) Preparation method: Microcrystalline wax, mica, burdock seed oil, isononyl isononanoate, jojoba seed oil and polyglycerol-3-diisostearate are mixed, stirred and dissolved, and heated and stirred to 82°C to disperse / dissolve evenly;

[0142] Hydroxypropyl tetrahydropyranotriol, sodium hyaluronate, glucomannan and water are mixed, stirred and dissolved, and heated and stirred to 82°C until uniformly dispersed / dissolved;

[0143] Mix the two ingredients thoroughly, add the lip care sunscreen multi-chamber microcapsules, keep warm and stir to disperse evenly, cool the paste to 62°C, pour it into a mold or tube to form and discharge, and obtain the sunscreen revitalizing lip balm.

[0144] Example 4

[0145] Moisturizing effect:

[0146] Samples: Example 3, Comparative Example 1, and commercially available samples.

[0147] Test objective: A single-center open trial, using subjective evaluations from more than 30 people after using the product, with before-and-after comparisons, to verify the product's efficacy.

[0148] Experimental participants: This test included 30 healthy female subjects aged 20-50 years. Volunteers with slight fine lines, dry lines or more obvious wrinkles on their lips were preferred. The participants were divided into three groups and three samples were tested in each group.

[0149] Test method:

[0150] Before and after comparison, volunteers' lips were tested before product use, 8 hours after product use, 7 days after product use, and 14 days after product use to measure the moisture content of the lip stratum corneum.

[0151] Sample usage instructions: Testers should apply the sample to their lips once a day, applying it evenly once.

[0152] Testing instruments:

[0153] Multi-probe skin testing system (Courage & Khazaka, Germany): Water content Corneometer probe for measuring moisture content.

[0154] Change rate of skin stratum corneum moisture content (au) (%) = (Measured value - Initial value) / Initial value × 100%

[0155] The specific results are shown in the table below.

[0156] Change rate of skin stratum corneum water content (au) 8H 7 days 14 days Example 3 7.24％ 14.43％ 12.43％ Comparative Example 1 3.47％ 9.36％ 7.13％ Commercially available products 5.37％ 10.45％ 8.91％

[0157] The water content of the stratum corneum is a commonly used indicator reflecting moisturizing effects. The data in the table above show that the components of this invention have good skin moisturizing capabilities.

[0158] Barrier repair effect:

[0159] Samples: Example 3, Comparative Example 1, and commercially available samples.

[0160] Test objective: A single-center open trial, using instrument testing with more than 30 people to verify the product's efficacy by comparing before and after use.

[0161] Experimental participants: This test included 30 healthy female subjects aged 20-50 years. Volunteers with slight fine lines, dry lines or more obvious wrinkles on their lips were preferred. The participants were divided into three groups and three samples were tested in each group.

[0162] Test method:

[0163] Before and after comparison, tests were conducted on the volunteers' lips before product use, 8 hours after product use, 7 days after product use, and 14 days after product use to measure transepidermal water loss. Sample usage method: Test participants applied the sample to their lips once daily.

[0164] Testing instruments:

[0165] Multi-probe skin testing system (Courage & Khazaka, Germany): Transdermal dehydration Tewameter probe.

[0166] The test results are as follows:

[0167] Changes in skin TEWL values 8H difference 7D difference 14-day difference Example 3 -15 -17 -21 Comparative Example 1 -11 -13 -15 Commercially available products -7 -8 -12

[0168] Transdermal water loss (TEWL), also known as transdermal water loss, is a commonly used indicator reflecting the function of the stratum corneum barrier. When the skin's stratum corneum is abnormal, damaged, or poorly cared for, the skin barrier will be impaired, leading to the skin losing its protective and moisturizing functions.

[0169] The data in the table above show that the components of this invention have good skin repair capabilities, can significantly reduce transepidermal water loss, and repair the skin.

[0170] Example 5

[0171] Anti-aging function:

[0172] Samples: Example 3, Comparative Examples 1, 2, 3, and 4

[0173] This test used human skin fibroblasts (HFF-1 cells) as model cells to determine the migration ability of fibroblasts under UVB damage.

[0174] HFF-1 cells were divided into groups of 2.0 × 10⁻⁶. 5 Inoculate 70 μL per well into a culture-inserts (2-well, ibidi) 12-well plate at a density of 1 / mL, culture in complete medium for 24 h, remove the medium, and wash 3 times with PBS.

[0175] Then add 100 μL of PBS buffer to each well, and the sample (20 mg / cm²) is added. 2 Apply evenly to the 12-hole plate cover, cover the blank group with aluminum foil, and leave the model group untreated.

[0176] Then, it was irradiated for 5 minutes under a UVB (308nm) crosslinker, with a total energy of 40mJ / cm². 2 The supernatant was removed, and the medium was replaced with 5% FBS DMEM. The culture was continued for 24 hours. Images were taken and processed using ImageJ, and the scratch area was calculated.

[0177] See results Figure 3 The results indicate that the model group had weak migration ability after UVB irradiation, but the blank group without UVB stimulation closed the scratch area to a certain extent after 24 hours of culture. The sample in Example 3 also had cell migration ability compared with the model group, and the significance was **P<0.001.

[0178] There was no significant difference between the control group (Comparative Example 1) and the model group. Control groups 2, 3, and 4 showed some cell migration ability, but none were as effective as Example 3, indicating that the combination of the three active ingredients had a synergistic effect.

[0179] Example 6

[0180] Sun protection effect:

[0181] Samples: Example 3, Comparative Example 1.

[0182] This test used immortalized human epidermal cells (HaCaT cells) as model cells to determine the effect of the sample on the viability of skin cells under UVB damage.

[0183] HaCaT cells were 1.0 × 10⁻⁶ 5 At a density of 100 μL / mL, 100 μL was inoculated into each well of a 96-well plate, cultured in complete culture medium for 24 h, then the culture medium was removed and the plate was washed three times with PBS.

[0184] Then add 100 μL of PBS buffer to each well, and the sample (20 mg / cm²) is added. 2 The coating was evenly applied to the cap of a 96-well plate. The blank control group was shielded with aluminum foil. The plate was then irradiated with UVB (308nm) for 15 minutes using a UV crosslinker, with a total energy of 120mJ / cm². 2 ;

[0185] Remove the supernatant, replace with serum-free DMEM medium, and continue culturing for 24 hours;

[0186] After washing three times with PBS, 100 μL of 0.5 mg / mL MTT solution (prepared with DMEM) was added to each well. After incubation for 4 h, the solution was removed and 100 μL of LDMSO was added. The absorbance was measured at 490 nm.

[0187] Cell viability is calculated according to the following formula:

[0188] Cell viability (%) = As / Ac × 100%, where As is the absorbance of the test sample and Ac is the absorbance of the blank group.

[0189] See results Figure 4 This indicates that Example 3 showed a significant difference compared to the model group and possessed the function of preventing UVB to protect keratinocytes. However, the cell viability of the sample in Comparative Example 1 showed no significant difference compared to the model group.

[0190] As demonstrated by the moisturizing and barrier repair effects tests, anti-aging experiments, and sun protection tests, the sun-protective and revitalizing lip balm of this invention has multiple functions, with significantly better moisturizing, nourishing, repairing, and anti-inflammatory soothing effects than commercially available products. It also has anti-aging and sun protection effects. The active ingredient, natural red, achieves a red effect without the need for pigments, making it a multi-functional sun-protective and revitalizing lip balm.

[0191] This invention discloses a multi-compartment microcapsule for lip care and sun protection, which combines three effective ingredients: styracin, astaxanthin, and pyrrolidinequinoline quinone, providing anti-aging and sun protection effects. This invention utilizes a combination of nanotechnology and low-temperature spray freezing technology to produce the multi-compartment microcapsule, enabling enzyme-controlled release and significantly enhancing the transdermal absorption and bioavailability of the active ingredients.

[0192] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the present invention.

Claims

1. A lip care sunscreen multi-compartment microcapsule characterized by: Including lycorine, astaxanthin, pyrroloquinoline quinone, candelilla wax, shea butter, lauryl laurate, zein, hydrolyzed corn starch octenyl succinate, silica, and PPG-3 benzyl ether myristate. Based on the percentage by weight of the raw materials, the following components are present: 0.1%–5% pyrrolizin, 0.1%–5% astaxanthin, 0.1%–5% pyrroloquinoline quinone, 5%–20% candelilla wax, 5%–20% shea butter, 5%–10% lauryl laurate, 1%–5% zein, 20%–40% hydrolyzed corn starch octenyl succinate, 10%–20% silica, and 5%–10% PPG-3 benzyl ether myristate. The preparation method of the lip care sunscreen multi-compartment microcapsule includes heating styrax, astaxanthin, pyrroloquinoline quinone, candelilla wax, and shea butter until completely mixed, which is phase A; Lauryl laurate, PPG-3 benzyl ether myristate, and water were heated until completely miscible to form phase B. Phase A was slowly added to phase B at 80°C and stirred until homogeneous. Then, the mixture was homogenized under high pressure at 800 bar to obtain phase C. Zeat protein and hydrolyzed corn starch octenyl succinate were dissolved in deionized water and hydrated overnight to obtain a transparent liquid. Then, the liquid was heated and stirred in a water bath at 90°C, silica was added, and the mixture was stirred until homogeneous to obtain phase D. Mix phase C and phase D, stir evenly, and put into a micro high-pressure atomizing pump. Use an open container to hold liquid nitrogen, then aim the nozzle of the atomizing pump at the liquid nitrogen and start the pump to spray. After spraying, the open container was placed in a freeze dryer and vacuum dried to obtain multi-chamber microcapsules.

2. The lip care suncare multi-compartment microcapsule of claim 1, wherein: The multi-chamber microcapsules enable enzyme-controlled release.

3. The lip care sunscreen multi-compartment microcapsule as described in claim 1, characterized in that: The heating temperature during the preparation of phase A is 80°C.

4. The lip care sunscreen multi-compartment microcapsule as described in claim 1, characterized in that: The heating temperature during the preparation of phase B is 80°C.

5. The lip care sunscreen multi-compartment microcapsule as described in claim 4, characterized in that: The high-pressure homogenization process for preparing the C phase is repeated 3 to 5 times.

6. The lip care sunscreen multi-compartment microcapsule as described in claim 5, characterized in that: The nozzle used for spraying is 0.2mm.

7. The use of the lip care sunscreen multi-compartment microcapsule as described in claim 1 or 2 in the preparation of a sunscreen and revitalizing lip balm.

8. The application as described in claim 7, characterized in that: The sunscreen and revitalizing lip balm comprises the following components by weight: Lip care sunscreen multi-compartment microcapsules 10%–30%, microcrystalline wax 10%–30%, mica 10%–15%, burdock seed oil 2%–10%, isononyl isononanoate 2%–10%, jojoba seed oil 2%–10%, polyglycerol-3-diisostearate 2%–5%, hydroxypropyl tetrahydropyranotriol 0.1%–2%, sodium hyaluronate 0.1%–2%, glucomannan 0.1%–2%, water to 100%.

9. The application as described in claim 8, characterized in that: The preparation method of the sunscreen and revitalizing lip balm includes, Microcrystalline wax, mica, burdock seed oil, isononyl isononanoate, jojoba seed oil, and polyglycerol-3-diisostearate were mixed, stirred, dissolved, and heated until homogeneous to obtain the first phase. Hydroxypropyltetrahydropyranotriol, sodium hyaluronate, glucomannan and water were mixed, stirred and dissolved, and heated until uniformly dissolved to obtain the second phase. Mix the first and second phases evenly, add the lip care sunscreen multi-chamber microcapsules, keep warm and stir evenly, cool the paste to 62°C, pour it into a mold or tube to form and discharge, and obtain the sunscreen and revitalizing lip balm.

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