Method for regulating activity of a pyrus self-incompatible gene pps-rnase promoter

By inhibiting the synthesis of brassinolide in the pear flower style and reducing the activity of the PpS-RNase gene, the problems of self-incompatibility and intergeneric hybridization difficulties in pear can be solved, thereby simplifying the management of pear breeding and improving the hybridization success rate.

CN119876456BActive Publication Date: 2026-08-07YANGZHOU UNIV
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Patent Information

Application Number
CN202510062023.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-01-15
Publication Date
2026-08-07
Estimated Expiration
2045-01-15

AI Technical Summary

Technical Problem

Pear's self-incompatibility makes pear orchard management difficult, and interbreeding between pear and apple genera is challenging, resulting in high pollen tube mortality. Existing technologies lack effective PpS-RNase gene regulation measures.

Method used

To reduce the PpS-RNase promoter activity in pear flower columns by inhibiting the synthesis of endogenous brassinolide, brassinolide was used as an inhibitor of brassinolide synthesis to treat pear flower columns and reduce the expression of the PpS-RNase gene.

Benefits of technology

Significantly reducing the expression of the pear PpS-RNase gene overcomes pear self-incompatibility, improves the success rate of intergeneric hybridization, and provides new ideas for pear breeding.

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Abstract

The application discloses a method for regulating activity of a pear self-incompatibility gene PpS-RNase promoter and belongs to the field of plant genetic engineering. The application determines a 1284bp promoter sequence of the pear PpS-RNase gene, and verifies that the activity of the pear PpS-RNase gene promoter is induced by brassinosteroid in a stable transformation system of Arabidopsis thaliana, and the synthesis of endogenous brassinosteroid can be inhibited to significantly reduce the expression of the pear PpS-RNase gene, thereby providing a new idea for solving pear self-incompatibility mediated by the pear PpS-RNase gene and interspecific hybrid breeding.
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