Preparation method, product and application of glass rose extract

Abstract

This invention provides a method for preparing Grasse rose extract, a product thereof, and its application, belonging to the field of cosmetic raw material technology. The method includes the following steps: Grasse rose is mixed with a solvent, and then flash extracted to obtain Grasse rose extract; wherein the solvent is an aqueous solution of butylene glycol. The Grasse rose extract prepared by this method has higher contents of astragaloside, ellagic acid, and isoquercitrin, and the ratio of these three components is more conducive to reducing melanin content and tyrosinase activity in melanocytes, providing a basis for its application in whitening cosmetics.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic raw material technology, and relates to a preparation method, product and application of Grasse rose extract. Background Technology

[0002] Grasse rose, also known as the centifolia rose (Rosa Centifolia L.), is a plant belonging to the genus Rosa in the family Rosaceae. It produces nodding flowers in late June or late July, solitary or rarely clustered, without bracts; often double-petaled; fragrant; the petals turn a translucent pink from the curve, very double, goblet-shaped, becoming looser when fully dispersed, revealing the tightly packed petals. The pedicels are slender, curved, and densely covered with glandular hairs; the sepals are ovate, with indistinct leaf-like tips, spreading, longer than the flower bud, and covered by the pedicel and bracts, which, like the pedicel and bracts, possess sticky aromatic glands. Grasse rose is an internationally renowned oil-producing rose, an important source plant for extracting cosmetic ingredients.

[0003] In his thesis "Research on Chemical Constituents and Quality Standards of Rose" (Master's thesis, Shandong University of Traditional Chinese Medicine, published online May 29, 2014), Ma Changhao conducted a preliminary study on the chemical components of dried flower buds of the perennial shrub rose (Rosa rugosa Thunb.), belonging to the genus Rosaceae. Through spectroscopic and chromatographic identification, six compounds were ultimately identified: gallic acid, protocatechuic acid, kaempferol, kaempferol-8-methyl ether, isoquercitrin, and astragaloside. Using gallic acid and hyperoside as reference standards, a high-performance liquid chromatography (HPLC) fingerprint of rose was established, providing a reference for rose identification and quality control. The ribosomal rDNA ITS sequences of different cultivated rose varieties were determined, providing a molecular biological basis for rose variety identification and germplasm resource optimization.

[0004] Chinese invention patent CN119032155A discloses a method for preparing fragrance extracts and / or absolutes, which involves contacting specific, fresh, withered, or dried solid natural materials with a first solvent system comprising at least one "green" alkane solvent containing at least eight carbon atoms. Another subject of this technology is the fragrance extract and absolute obtained by this preparation method, a composition comprising the extract and / or absolute, and the use of the "green" alkane solvent for extracting the extract and / or absolute without a solvent-based chemical odor. This disclosure does not investigate the content, proportion, or relationship between the active ingredient in the extract obtained by the extraction method and its whitening effect.

[0005] In summary, the optimal ratio of various whitening active ingredients in Grasse rose is still unclear in the current technology, and a preparation process for Grasse rose extract with a better ratio of each ingredient to enhance the whitening effect has not yet been provided. Summary of the Invention

[0006] In view of the above-mentioned problems in the prior art, the purpose of this invention is to provide a method for preparing Grasse rose extract, a product thereof, and its application. Using the melanin content and tyrosinase activity of B16-F10 cells as indicators, various ratios of the main rose monomers astragaloside, ellagic acid, and isoquercitrin were screened, successfully determining the optimal combination for whitening effect. Based on this, the Grasse rose extraction process was further screened and optimized. While maintaining this optimal monomer ratio, the monomer content in the Grasse rose extract was effectively increased by changing the preparation conditions in the extraction process, providing a Grasse rose extract cosmetic raw material with significant whitening effect, and providing a basis for its application in cosmetics.

[0007] To achieve the above-mentioned objectives, the present invention provides a method for preparing Grasse rose extract, comprising the following steps:

[0008] Grasse rose is mixed with a solvent and flash extracted to obtain Grasse rose extract;

[0009] The solvent is an aqueous solution of butanediol.

[0010] Preferably, the Grasse rose is dried Grasse rose; and the butanediol is 1,3-butanediol.

[0011] Preferably, the butanediol aqueous solution is a butanediol aqueous solution with a weight concentration of 20%-40%.

[0012] More preferably, the butanediol aqueous solution is a butanediol aqueous solution with a weight concentration of 30%.

[0013] Preferably, the ratio of Grasse rose to solvent is 1:60-80, in g / mL.

[0014] More preferably, the ratio of the Grasse rose to the solvent is 1:70, in g / mL.

[0015] Preferably, the flash extraction time is 20-40 seconds, and the flash extraction voltage is 80-100V.

[0016] More preferably, the flash extraction time is 30 seconds and the flash extraction voltage is 80V.

[0017] Preferably, the Grasse roses are pulverized before being mixed with the solvent.

[0018] Preferably, the flash extraction is followed by filtration and drying.

[0019] More preferably, the filtration is a filter plate filtration.

[0020] As an example of the present invention, the filter plate has a pore size of 0.45 μm.

[0021] More preferably, the drying is freeze drying.

[0022] On the other hand, the present invention provides Grasse rose extract prepared by the above-described preparation method.

[0023] Preferably, the Grasse rose extract contains ellagic acid, isoquercitrin, and astragaloside.

[0024] More preferably, in the Grasse rose extract, the concentration of ellagic acid is 50-95 mg / g, the concentration of isoquercitrin is 21-48 mg / g, and the concentration of astragaloside is 23-143 mg / g.

[0025] More preferably, the concentration ratio of astragaloside, ellagic acid, and isoquercitrin is 2.5-3.5::1.5-2.5:0.8-1.2, more preferably 2.9-3.1:1.9-2.1:0.9-1.1, and most preferably 3:2:1.

[0026] Furthermore, this invention provides the application of the Grasse rose extract prepared by the above method in cosmetics.

[0027] Finally, the present invention provides a cosmetic comprising Grasse rose extract prepared by the above-described method.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] This invention provides a method for preparing Grasse rose extract using flash extraction. Using the melanin content and tyrosinase activity of B16-F10 cells as indicators, this invention screened various ratios of the main components of Grasse rose—astragalin, ellagic acid, and isoquercitrin—and successfully determined the optimal combination for whitening effect. Based on the optimal ratio of astragalin, ellagic acid, and isoquercitrin, flash extraction technology was applied to the extraction of Grasse rose, and the process conditions were optimized, effectively increasing the monomer content in the Grasse rose extract. This provides a Grasse rose extract cosmetic raw material with significant whitening effects, providing a basis for its application in cosmetics. Detailed Implementation

[0030] Terminology and Declarations of this Invention:

[0031] 1. Articles “a,” “a kind,” and “the”: These include plural objects unless otherwise explicitly specified as a single (kind) object.

[0032] 2. Numerical Range: Unless otherwise expressly stated, all ranges or ratios disclosed herein shall be construed as including any and all subranges or subratios contained herein. For example, a stated range or ratio of 1 to 30 shall be considered to be included between the minimum value of 1 and the maximum value of 30, and includes any subranges or subratios, integers, decimals, or subranges or subratios consisting of integers or decimals, including endpoints.

[0033] The following non-limiting embodiments are intended to enable those skilled in the art to gain a more comprehensive understanding of the present invention, but do not limit the invention in any way. The following description is merely an exemplary illustration of the scope of protection of the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, which should also fall within the scope of protection of the present invention.

[0034] The present invention will be further described below by way of specific embodiments. Unless otherwise specified, all chemical reagents used in the embodiments of the present invention were obtained through conventional commercial means. Unless otherwise specified, all contents mentioned below are mass contents. Unless otherwise specified, it is understood that the process was carried out at room temperature.

[0035] The sources of some reagents in the following examples and comparative examples are shown in Table 1 below:

[0036] Table 1

[0037] name Manufacturer / Source Item number / specification 1,3-Butanediol Shanghai Aladdin Biochemical Technology Co., Ltd. 1kg Astragaloside Shanghai Yuanye Biotechnology Co., Ltd. 20mg Ellagic acid Shanghai Yuanye Biotechnology Co., Ltd. 20mg Isoquercitrin Shanghai Yuanye Biotechnology Co., Ltd. 20mg

[0038] The information on some of the instruments and equipment used in the following embodiments and comparative examples is shown in Table 2 below:

[0039] Table 2

[0040]

[0041]

[0042] In this invention, high-performance liquid chromatography (HPLC) was used to detect the content of astragalin and ellagic acid (isoquercetin) in the extracts of Grasse rose in each example / comparative example. The detection method is as follows:

[0043] (1) Chromatographic conditions and system suitability test: The Agilent 1260 Infinity high performance liquid chromatography system was used to perform quantitative analysis of the component content of the sample.

[0044] Instrument model: Agilent 1260; Column: ZORBAX SB-C18 (4.6mm×250mm, 5μm); Injection volume: 10μL; Detection wavelength: 254nm; Mobile phase: A: 0.3% formic acid aqueous solution; B: acetonitrile; Flow rate: 1.0mL / min.

[0045] The elution procedure is shown in Table 3 below.

[0046] Table 3

[0047] Time (min) Mobile phase A (%) Mobile phase B (%) 0 95 5 5 95 5 10 90 10 15 85 15 25 85 15 35 80 20 45 70 30 55 60 40

[0048] (2) Preparation of reference solutions: Accurately weigh ellagic acid, isoquercitrin and astragaloside, add methanol to prepare three solutions of 0.5 mg / ml. Then take 300 μl of each of the three 0.5 mg / ml solutions, mix them evenly and filter them through a 0.22 μm organic microporous filter. Take the filtrate to obtain the standard solution.

[0049] (3) Preparation of test solution: Take an appropriate amount of sample, weigh it accurately, prepare a 1 mg / ml test solution with methanol, pass it through a 0.22 μm organic microporous filter, and take the filtrate.

[0050] (4) Determination method: Accurately pipette 10 μL of the reference solution and the test solution into the liquid chromatograph and determine the result.

[0051] Experimental Example 1

[0052] Evaluation of the effects of mixtures of different proportions of astragaloside, ellagic acid and isoquercitrin on melanin content and tyrosinase activity in B16-F10 cells.

[0053] Astragaloside, ellagic acid, and isoquercitrin are all active ingredients found in Grasse rose. To investigate the optimal ratio of these three ingredients for optimal whitening effects, the following experiment was conducted.

[0054] Group 1: Accurately weigh astragalin and dissolve it in DMSO liquid to prepare a stock solution. Prepare solutions with fresh culture medium at concentrations of 10 μg / mL, 5 μg / mL, and 1 μg / mL.

[0055] Group 2: Accurately weigh ellagic acid and dissolve it in DMSO liquid to prepare a stock solution. Use fresh culture medium to prepare liquids with concentrations of 10 μg / mL, 5 μg / mL, and 1 μg / mL.

[0056] Group 3: Accurately weigh isoquercitrin and dissolve it in DMSO liquid to prepare a stock solution. Prepare solutions with fresh culture medium at concentrations of 10 μg / mL, 5 μg / mL, and 1 μg / mL.

[0057] Group 4: The stock solutions of astragaloside, ellagic acid and isoquercitrin were mixed in a ratio of 1:1:1 and prepared into liquids with concentrations of 10 μg / mL, 5 μg / mL and 1 μg / mL using fresh culture medium.

[0058] Group 5: The stock solutions of astragaloside, ellagic acid and isoquercitrin were mixed in a ratio of 2:1:1 and prepared into liquids with concentrations of 10 μg / mL, 5 μg / mL and 1 μg / mL using fresh culture medium.

[0059] Group 6: The stock solutions of astragaloside, ellagic acid and isoquercitrin were mixed in a ratio of 3:1:1 and prepared into liquids with concentrations of 10 μg / mL, 5 μg / mL and 1 μg / mL using fresh culture medium.

[0060] Group 7: The stock solutions of astragaloside, ellagic acid and isoquercitrin were mixed in a ratio of 1:2:1 and prepared into liquids with concentrations of 10 μg / mL, 5 μg / mL and 1 μg / mL using fresh culture medium.

[0061] Group 8: The stock solutions of astragaloside, ellagic acid and isoquercitrin were mixed in a ratio of 1:3:1 and prepared into liquids with concentrations of 10 μg / mL, 5 μg / mL and 1 μg / mL using fresh culture medium.

[0062] Group 9: The stock solutions of astragaloside, ellagic acid and isoquercitrin were mixed in a ratio of 2:2:1 and prepared into liquids with concentrations of 10 μg / mL, 5 μg / mL and 1 μg / mL using fresh culture medium.

[0063] Group 10: The stock solutions of astragaloside, ellagic acid and isoquercitrin were mixed in a ratio of 3:2:1 and prepared into liquids with concentrations of 10 μg / mL, 5 μg / mL and 1 μg / mL using fresh culture medium.

[0064] Group 11: The stock solutions of astragaloside, ellagic acid and isoquercitrin were mixed in a ratio of 2:3:1 and prepared into liquids with concentrations of 10 μg / mL, 5 μg / mL and 1 μg / mL using fresh culture medium.

[0065] The experimental methods for studying the effects of the above 11 solutions on the melanin inhibition rate and tyrosinase inhibition rate of B16-F10 cells are as follows.

[0066] 1. NaOH method for detecting melanin inhibition rate in B16-F10 cells

[0067] (1)Planning

[0068] Collect B16-F10 cell solution at 1×10 6 Cells / well were seeded at a density of 6 cells / well in 6-well plates (with sterile PBS added to the remaining wells) and incubated in an incubator at 37°C with 5% CO2.

[0069] (2) Sample loading

[0070] When 80%-90% of the B16-F10 cells adhere to the culture medium, discard the culture medium and wash twice with PBS buffer. Prepare samples of the corresponding concentrations using the culture medium (for samples insoluble in the culture medium, prepare a stock solution using DMSO), and sterilize by membrane filtration. If the cells secrete melanin normally, load the samples directly. The blank group is the model group. If the cells secrete less melanin, add α-MSH to stimulate melanin secretion. The group containing only α-MSH is the model group, and the group without α-MSH is the blank group. Each group has two replicates.

[0071] (3) Cell lysis

[0072] Incubate for 24 hours. Aspirate the liquid from the wells, wash twice with PBS, add the sample again, and incubate for 12 hours. Aspirate the liquid from the wells. Add PBS slowly along the wall, wash twice, discard the PBS, and aspirate any remaining PBS with a 200 μL pipette. Add 400 μL of 1 mmol / L LPMSF cell lysis buffer to each well, incubate on ice for 5 minutes, and collect the cell suspension in a centrifuge tube. Centrifuge at 12000 rpm for 10 minutes at 4°C.

[0073] (4) Melanin determination

[0074] Transfer the supernatant to a new centrifuge tube and determine the total cellular protein content. Melanin precipitates at the bottom of the centrifuge tube. Add 300 μL of 1M NaOH lysis buffer (containing 10% DMSO) to the precipitate, vortex thoroughly, and incubate in a metal bath at 85°C for 20 min to completely dissolve the melanin precipitate. After removing the tube, vortex thoroughly again, and transfer 100 μL per well to a 96-well plate with three replicates. Measure the OD value at 405 nm.

[0075] (5) BCA method for determining cell protein concentration

[0076] Prepare the BCA solution according to the BCA kit instructions. Add 25 μL of the lysed total cell protein solution and water to a 96-well plate, then add 200 μL of BCA solution, making three sub-wells per tube. Incubate at 37°C for 30 min on a shaker, and measure the OD value at 562 nm. (Use the BCA kit and follow the kit instructions.) Calculate the total protein concentration based on the standard curve.

[0077] Melanin inhibition rate = 1 - (OD value of experimental group / protein concentration) / (OD value of model group / protein concentration)

[0078] The experimental results of melanin inhibition rate of each group are shown in Table 4 below (mean ± standard deviation).

[0079] Table 4

[0080]

[0081]

[0082] It is evident that in group 10 (a ratio of astragaloside, ellagic acid, and isoquercitrin of 3:2:1), the melanin inhibition rate at different concentrations (10 μg / mL, 5 μg / mL, and 1 μg / mL) was the highest among all groups at the same concentration. This indicates that a ratio of astragaloside, ellagic acid, and isoquercitrin of 3:2:1 exhibits the optimal melanin inhibition effect.

[0083] 2. Determination of tyrosinase activity by L-DOPA oxidation method

[0084] Add 120 μg of total protein supernatant to a 1.5 mL centrifuge tube, and bring the volume to 400 μL with PBS buffer. Vortex mix and then add the mixture sequentially to a 96-well plate. Add 100 μL of 0.1% L-DOPA to each well. Set up three replicates for each group and incubate at 37°C in the dark for 1 h. Measure the OD value at 475 nm using a microplate reader.

[0085] Tyrosinase inhibition rate = 1 - (experimental group / model group)

[0086] The experimental results of tyrosinase inhibition in each group are shown in Table 5 below (mean ± standard deviation).

[0087] Table 5

[0088]

[0089] It is evident that in group 10 (a ratio of astragaloside, ellagic acid, and isoquercitrin of 3:2:1), the tyrosinase inhibition rate at different concentrations (10 μg / mL, 5 μg / mL, and 1 μg / mL) was the highest among all groups at the same concentration. This indicates that a ratio of astragaloside, ellagic acid, and isoquercitrin of 3:2:1 exhibits the optimal tyrosinase inhibitory effect.

[0090] In summary, when the ratio of astragaloside, ellagic acid, and isoquercitrin is 3:2:1, the inhibition rates of melanin and tyrosinase in B16-F10 cells at concentrations of 10, 5, and 1 μg / mL are the highest, resulting in the best whitening effect.

[0091] Example 1

[0092] A method for preparing Grasse rose extract.

[0093] Weigh 5g of naturally air-dried Grasse rose petals and crush them. Add 350mL of 30% 1,3-butanediol aqueous solution at a material-to-liquid ratio of 1:70. Perform flash extraction for 30s (90V). Filter the extract through a 0.45μm filter plate. Take half of the extract and freeze-dry it to obtain Grasse rose extract 1.

[0094] Comparative Example 1

[0095] Grasse rose extract was prepared by microwave reflux extraction.

[0096] Weigh 5g of naturally air-dried Grasse rose petals and crush them. Add 350mL of 30% 1,3-butanediol aqueous solution at a material-to-liquid ratio of 1:70. Microwave reflux extraction was performed for 150min. After cooling to room temperature, the extract was filtered through a 0.45μm filter plate and rotary evaporated to below 87.5mL to allow the ethanol to fully evaporate. 1,3-butanediol aqueous solution was added to 350mL. Half of the extract was freeze-dried to obtain Grasse rose extract D1.

[0097] Comparative Example 2

[0098] Grasse rose extract was prepared by microwave reflux extraction.

[0099] Compared to Comparative Example 1, the difference was that 350 mL of 30% 1,3-butanediol aqueous solution was replaced with 350 mL of deionized water; all other aspects remained the same. The resulting extract was designated as Grasse Extract D2.

[0100] Comparative Example 3

[0101] Grasse rose extract was prepared by ultrasound-assisted extraction.

[0102] Weigh 5g of naturally dried Grasse rose petals and crush them. Add 350mL of 75% ethanol solution at a material-to-liquid ratio of 1:70. Extract with ultrasonic assistance for 50min. After cooling to room temperature, filter the extract through a 0.45μm filter plate. Take half of the extract and freeze-dry it to obtain Grasse rose extract D3.

[0103] The contents of astragaloside, ellagic acid and isoquercitrin in the Grasse rose extracts obtained in Example 1 and Comparative Examples 1-3 were detected by HPLC. The results are shown in Table 6 below (mean ± standard deviation).

[0104] Table 6

[0105] Ellagic acid (mg / g) Isoquercetin (mg / g) Astragaloside (mg / g) Grasse Rose Extract 1 69.88±0.11 34.19±0.62 102.61±0.74 Grasse Rose Extract D1 92.10±0.39 44.48±0.15 90.48±0.11 Grasse Rose Extract D2 17.60±0.56 17.19±0.09 52.78±0.45 Grasse Rose Extract D3 29.5±0.19 39.99±0.55 99.03±0.15

[0106] Grasse rose extract 1, Grasse rose extract D1, Grasse rose extract D2, and Grasse rose extract D3 were prepared into mixtures with weight concentrations of 2.5%, 1.25%, and 0.63% respectively using fresh culture medium. The inhibition rates of melanin and tyrosinase in each mixture on B16-F10 cells were detected using the method provided in Example 1. The experimental results of melanin inhibition rate are shown in Table 7 below (mean ± standard deviation).

[0107] Table 7

[0108]

[0109] The experimental results of tyrosinase inhibition rate are shown in Table 8 below (mean ± standard deviation).

[0110] Table 8

[0111]

[0112] The results showed that the proportions of astragalin, ellagic acid, and isoquercitrin in the Grasse rose extract obtained by flash extraction in Example 1 were closest to the optimal proportions for whitening effect, and it also exhibited the highest inhibition rates of melanin and tyrosinase in B16-F10 cells. These effects could not be achieved using microwave reflux or ultrasonic extraction methods.

[0113] Example 2

[0114] Weigh 5g of naturally air-dried Grasse rose petals and crush them. Add 350mL of 30% 1,3-butanediol aqueous solution at a material-to-liquid ratio of 1:70. Perform flash extraction for 30s (80V). Filter the extract through a 0.45μm filter plate. Take half of the extract and freeze-dry it to obtain Grasse rose extract 2g.

[0115] Example 3

[0116] Compared with Example 2, the only difference is that the voltage of the flash extraction is changed to 100V, and everything else is the same.

[0117] The resulting extract is designated as Grasse Rose Extract 3.

[0118] Example 4

[0119] Compared with Example 2, the only difference is that 300 mL of 30% 1,3-butanediol aqueous solution was added at a material-to-liquid ratio of 1:60; all other aspects are the same.

[0120] The resulting extract is designated as Grasse Rose Extract 4.

[0121] Example 5

[0122] Compared with Example 2, the only difference is that 400 mL of 30% 1,3-butanediol aqueous solution was added at a material-to-liquid ratio of 1:80; all other aspects are the same.

[0123] The resulting extract is designated as Grasse Rose Extract 5.

[0124] Example 6

[0125] Compared with Example 2, the only difference is that the flash extraction time is changed to 40 seconds, and everything else is the same.

[0126] The resulting extract is designated as Grasse Rose Extract 6.

[0127] Example 7

[0128] Compared with Example 2, the only difference is that the flash extraction time is changed to 20 seconds, and everything else is the same.

[0129] The resulting extract is designated as Grasse Rose Extract 7.

[0130] Example 8

[0131] Compared with Example 2, the only difference is that the concentration of the 1,3-butanediol aqueous solution is changed to 40%, and all other aspects are the same.

[0132] The resulting extract is designated as Grasse Rose Extract 8.

[0133] Example 9

[0134] Compared with Example 2, the only difference is that the concentration of the 1,3-butanediol aqueous solution is changed to 20%, and all other aspects are the same.

[0135] The resulting extract is designated as Grasse Rose Extract 9.

[0136] Comparative Example 4

[0137] Compared with Example 2, the only difference is that 1,3-butanediol is replaced with ethanol, and everything else is the same.

[0138] The resulting extract is designated as Grasse Rose Extract D4.

[0139] The contents of ellagic acid, isoquercitrin, and astragaloside in Grasse rose extracts 1-9 and D4 were determined by HPLC. The results are shown in Table 9 below (mean ± standard deviation):

[0140] Table 9

[0141] Ellagic acid (mg / g) Isoquercetin (mg / g) Astragaloside (mg / g) Grasse Rose Extract 1 69.88±0.11 34.19±0.62 102.61±0.74 Grasse Rose Extract 2 94.88±0.11 47.19±0.62 141.61±0.74 Grasse Rose Extract 3 72.13±0.29 36.01±0.11 107.18±0.21 Grasse Rose Extract 4 67.51±0.39 31.29±0.15 99.13±0.12 Grasse Rose Extract 5 61.31±0.26 30.12±0.12 92.71±0.21 Grasse Rose Extract 6 53.41±0.19 24.13±0.13 79.11±0.41 Grasse Rose Extract 7 57.34±0.31 27.21±0.21 86.10±0.16 Grasse Rose Extract 8 50.27±0.23 26.12±0.31 53.17±0.62 Grasse Rose Extract 9 63.61±0.15 21.41±0.16 23.63±0.15 Grasse Rose Extract D4 34.55±0.15 26.22±0.48 67.34±0.51

[0142] The results showed that the contents of astragaloside, ellagic acid, and isoquercitrin in Grasse rose extract 2 were significantly increased and met the optimal ratio.

[0143] In summary, the highest melanin inhibition rate and tyrosinase inhibition rate were observed in B16-F10 cells when the ratio of astragaloside, ellagic acid, and isoquercitrin was 3:2:1. When the extract of Grasse rose was prepared using the process described in Example 2, the ratio of astragaloside, ellagic acid, and isoquercitrin in the extract met the optimal whitening effect, and the content of these three monomers was significantly increased compared to other examples. This invention is the first to determine the optimal whitening ratio of astragaloside, ellagic acid, and isoquercitrin in rose monomers, and provides a novel preparation process for Grasse rose extract, namely flash extraction. The Grasse rose extract prepared using this process meets the optimal ratio for whitening effect, and the content is significantly increased. This provides a cosmetic raw material for Grasse rose extract with whitening effects, providing a basis for its application in cosmetics.

[0144] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A method for preparing Grasse rose extract, characterized in that, Includes the following steps: Grasse rose is mixed with a solvent and flash extracted to obtain Grasse rose extract; The solvent is an aqueous solution of butanediol; The Grasse rose extract contains ellagic acid, isoquercitrin and astragaloside; The concentration ratio of astragaloside, ellagic acid, and isoquercitrin is 2.5-3.5:1.5-2.5:0.8-1.2; The Grasse rose is Grasse rose petals, the butanediol is 1,3-butanediol, the concentration of the butanediol aqueous solution is 30%, and the flash extraction time is 30 seconds.

2. The preparation method according to claim 1, characterized in that, The Grasse roses mentioned are dried Grasse roses.

3. The preparation method according to claim 1, characterized in that, The ratio of Grasse rose to solvent is 1:60-80, in g / mL.

4. The preparation method according to claim 1, characterized in that, The voltage for the flash extraction is 80-100V.

5. The preparation method according to claim 4, characterized in that, The voltage for the flash extraction is 80V.

6. The preparation method according to claim 1, characterized in that, The Grasse roses are pulverized before being mixed with the solvent, and the flash extraction is followed by filtration and drying.

7. The preparation method according to claim 6, characterized in that, The filtration is a filter plate filtration, and the drying is freeze drying.

8. Grasse rose extract prepared by any one of the preparation methods of claims 1-7.

9. The use of Grasse rose extract prepared by any one of claims 1-7 in cosmetics.

10. A cosmetic product, characterized in that, Includes Grasse rose extract prepared by the preparation method according to any one of claims 1-7.

Images