Fusion protein specifically binding to type Z nucleic acid and use thereof
By designing Zα domain and Fc segment fusion protein Zα-Fc of ZBP1, the existing Z-NAs labeling tools are solved, and efficient and specific labeling of Z-DNA and Z-RNA is achieved, providing a more economical and effective labeling tool.
Patent Information
- Application Number
- CN202411844546.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2023-12-15
- Filing Date
- 2024-12-13
- Publication Date
- 2025-05-02
AI Technical Summary
Existing Z-NAs labeling tools, especially commercial antibody Z-22, are difficult to effectively recognize and label different types and sequences of levonucleic acids in vivo, and the preparation cost of monoclonal antibodies is high, resulting in insufficient recognition and high cost.
The two Zα domains of ZBP1 were designed and purified to fuse with the Fc segment of mouse immunoglobulin to form the fusion protein Zα-Fc. This fusion protein binds Z-NAs through the Zα domain and the Fc segment binds to fluorescent secondary antibodies, achieving efficient labeling of Z-NAs.
Zα-Fc fusion proteins are able to efficiently recognize and label Z-DNA and Z-RNA in vivo, providing more realistic Z-NAs signals, reducing preparation costs, and improving label specificity and broad spectrum.