Application of CsZAT6 gene or protein in regulating theanine synthesis

By regulating theanine synthesis through the CsZAT6 gene and its encoded protein, and utilizing overexpression and silencing techniques, the problem of unclear theanine synthesis mechanism was solved, and the theanine content was effectively regulated, thus constructing a tea tree model for efficient theanine synthesis or reduction.

CN120041488BActive Publication Date: 2025-10-31ANHUI AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202510218264.2
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-02-26
Publication Date
2025-10-31
Estimated Expiration
2045-02-26

AI Technical Summary

Technical Problem

The molecular regulatory mechanisms of the efficient synthesis and decomposition of theanine in tea plants are still unclear, and the role of CsZATs family genes in the synthesis of theanine in tea plants has not been reported.

Method used

By mining the CsZAT6 transcription factor, the synthesis of theanine can be regulated by the CsZAT6 gene and its encoded protein. The theanine synthesis pathway can be regulated by CsZAT6 gene overexpression or silencing technology, including CsZAT6 gene expression cassettes, overexpression vectors and inhibitors such as siRNA, to achieve the regulation of theanine content.

Benefits of technology

Significantly increasing or decreasing theanine content, constructing tea tree models rich in or low in theanine, provides a new pathway for efficient theanine synthesis, and identifies the CsZAT6 gene as a key target for theanine synthesis.

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Abstract

This invention provides the application of the CsZAT6 gene or protein in regulating theanine synthesis, belonging to the field of molecular breeding technology. Specifically, the application of the CsZAT6 gene or its encoded protein in regulating theanine synthesis involves either negatively regulating the expression level of the CsZAT6 gene to reduce theanine synthesis or positively regulating its expression level to promote theanine synthesis. The CsZAT6 gene is a potential target gene for theanine synthesis. Experimental results show that the CsZAT6 gene can respond to IAA treatment, regulating the content of ethylamine and theanine in tea plants by modulating the CsAlaDC promoter.
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