A pesticide composition and use thereof for controlling fusarium diseases

Abstract

The present application relates to a kind of pesticide composition and its purposes for preventing and treating fusarium diseases, comprising active ingredient A and active ingredient B, the active ingredient A is Cyclobutrifluram, active ingredient B is Bifemetstrobin, the mass ratio of active ingredient A and active ingredient B is 1:40~22:1.The fungicidal effect of the pesticide composition of the present application under certain mass ratio is not the simple superposition of the activity of each component, has high antibacterial activity to the disease caused by fusarium (Fusarium), synergistic effect is remarkable, is safe to crops, has the effect of yield increase and harvest protection.

Description

A pesticide composition and its use in controlling Fusarium diseases. Technical Field

[0001] This invention relates to the field of pesticide and fungicide technology, specifically to a pesticide composition and its use in controlling Fusarium diseases. Background Technology

[0002] Fusarium is an important pathogen causing many soil-borne and seed-borne diseases, such as wheat stem rot, corn stem rot, wheat scab, and wheat root rot. The pathogens have similar compositions and are suitable for similar chemical pesticides. In addition, most regions generally adopt crop rotation systems. Under this background, long-term use of a single type of chemical pesticide can easily lead to excessive pesticide residues in the farmland environment, resulting in a decline in crop quality and damage to the ecological environment. It can also easily cause pathogens to develop resistance.

[0003] Cyclobutrifluram (trifluoropyridine amide) is an SDHI fungicide and nematicide with a phenylcyclobutylpyridine amide structure reported by Syngenta (CAS Registry No.: 1644251-74-0). It has a broad spectrum and high efficacy, can prevent the infestation of fungi such as Fusarium and root-knot nematodes, and can be transported to the above-ground parts of plants to prevent early diseases.

[0004] Bifemetstrobin is a methoxyacrylate fungicide developed by Sumitomo Chemical Co., Ltd., CAS Registry Number: 2454319-63-0, Chinese chemical name: (2Z)-3-methoxy-2-[(4-methyl[1,1'-biphenyl]-3-yl)oxy]prop-2-enoic acid methyl ester. It is an inhibitor of the outer Qo site (cyt b gene) of cytochrome bc1 (panthenol oxidase) in the respiratory chain mitochondrial complex III. Unlike other methoxyacrylate fungicides, it has an oxygen atom inserted between the methoxyacrylate and the benzene ring, exhibiting high activity against diseases such as rice blast, cucumber anthracnose, cucumber powdery mildew, wheat leaf rust, and corn gray spot.

[0005] This invention has conducted in-depth research on Fusarium diseases. Numerous experimental results show that the combination of the two provides a strong defense against a variety of Fusarium pathogens, has a significant synergistic effect, and helps delay the development of resistance, which is of great significance for ensuring the safe production of crops. Summary of the Invention

[0006] Based on the above, the present invention aims to provide a pesticide composition, mainly used to control diseases caused by pathogenic fungi of the genus Fusarium in plants. This pesticide composition or its formulation can enhance efficacy, reduce dosage, prolong the duration of action, delay the development of resistance, and has high safety for crops.

[0007] This objective is achieved through the following technical solution: a pesticide composition comprising active ingredient A and active ingredient B, wherein active ingredient A is Cyclobutrifluram and active ingredient B is Bifemetstrobin;

[0008] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:40 to 22:1 or any value between the above values;

[0009] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:30 to 22:1;

[0010] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:25 to 20:1;

[0011] Furthermore, based on a total weight of 100 wt% of the pesticide composition, the sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 1 to 90 wt%.

[0012] Furthermore, based on a total weight of 100 wt% of the pesticide composition, the sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 5 to 80 wt%.

[0013] Furthermore, in addition to the active ingredient, the pesticide composition also contains agriculturally permissible auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, binders, or carriers.

[0014] Further, the wetting agent is selected from one or more of alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, soapberry powder, soapberry powder, SOPA, detergents, emulsifiers 2000 series, and wetting and penetrating agents F; and / or

[0015] Further, the dispersant is selected from one or more of the following: lignin sulfonate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers, and EO-PO graft copolymers; and / or

[0016] Further, the emulsifier is selected from one or more of the following: calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrene-phenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkylphenol ether phosphate; and / or

[0017] Furthermore, the thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and silica; and / or

[0018] Further, the disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0019] Furthermore, the antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0020] Furthermore, the defoamer is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of the fatty alcohols; and / or

[0021] Further, the solvent is selected from one or more of benzene, toluene, xylene, mesitylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, hydrocarbon carbonates, diesel oil, solvent oil, vegetable oil (soybean oil, corn oil, rapeseed oil, palm oil, etc.), vegetable oil derivatives, and water; and / or

[0022] Further, the preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon, and 1,2-benzisothiazolin-3-one; and / or

[0023] Further, the stabilizer is selected from one or more of the following: disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-methylphenol, triethanolamine oleate, epoxidized vegetable oil, kaolin, diatomaceous earth, bentonite, attapulgite, silica, talc, montmorillonite, and starch; and / or

[0024] Furthermore, the synergist is selected from synergistic phosphorus, synergistic ether; and / or

[0025] Furthermore, the carrier is selected from one or more of the following: ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.

[0026] Furthermore, the formulation of the pesticide composition is selected from solid formulations and / or liquid formulations and / or seed treatment formulations;

[0027] Furthermore, the solid dosage forms include powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules;

[0028] Furthermore, the liquid formulation includes soluble agents, colloids, oils, spreading oils, emulsions, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipids, suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspensions, microcapsule suspension-suspensions, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions;

[0029] Furthermore, the pesticide composition can be prepared into a pesticide-acceptable formulation, such as a microemulsion, emulsifiable concentrate, suspension concentrate, water-in-oil emulsion, water-dispersible granules, or seed treatment suspension.

[0030] The present invention also discloses the use of the pesticide composition described above in the prevention and control of plant diseases;

[0031] Furthermore, the plant disease is a plant disease caused by fungi;

[0032] Furthermore, the fungus is a member of the genus Fusarium.

[0033] Further, the *Fusarium* genus includes *Fusarium graminearum*, *Fusarium culmorum*, *Fusarium pseudograminearum*, *Fusarium oxysporum*, *Fusarium equiseti*, *Fusarium proliferatum*, *Fusarium chlamydosporum*, *Fusarium araliavenaceum*, *Fusarium solani*, *Fusarium asiaticum*, *Fusarium acuminatum*, *Fusarium moniliforme*, *Fusarium tricinctum*, *Fusarium poae*, *Fusarium lateritium*, and *Fusarium* pseudo-Verticillium. verticillioides), Fusarium concolor;

[0034] Furthermore, the Fusarium species mentioned are Fusarium graminearum and Fusarium culmorum;

[0035] Furthermore, the plant diseases caused by Fusarium are wheat stem base rot, corn stem base rot, wheat scab, and wheat root rot;

[0036] The specific pathogens causing wheat stem rot include: Fusarium graminearum, Fusarium culmorum, Fusarium pseudograminearum, Fusarium oxysporum, Fusarium equiseti, Fusarium proliferatum, Fusarium chlamydosporum, Fusarium ovalevenaceum, Fusarium solani, Fusarium asiaticum, and Fusarium acuminatum. This is a soil-borne fungal disease caused by Fusarium fungi infecting the base of wheat stems and can cause damage throughout the entire growth period. In the early stages of the disease, seeds fail to germinate properly, roots rot, and leaves turn yellow. As the disease progresses, browning occurs at the base of the stem, making it brittle and prone to breakage. Red or white mold forms at the nodes, ultimately leading to white ears, underdeveloped grains, or even no grains at all, severely impacting wheat yield. Furthermore, infection by the pathogen produces a series of biologically active secondary metabolites, primarily trichothecene toxins. These toxins inhibit the synthesis of cellular DNA, RNA, ribosomal proteins, and cellular proteins, activating ribotoxic stress responses. Ingestion of wheat containing these toxins poses a serious threat to the health and lives of humans and other mammals.

[0037] The specific pathogens causing corn stalk rot include: Fusarium graminearum, Fusarium culmorum, Fusarium proliferatum, Fusarium solani, Fusarium moniliforme, Fusarium verticillioides, and Fusarium concolor.

[0038] The pathogens causing wheat scab include: Fusarium graminearum, Fusarium culmorum, Fusarium oxysporum, Fusarium equiseti, Fusarium avenaceum, Fusarium asiaticum, Fusarium acuminatum, Fusarium moniliforme, Fusarium tricinctum, Fusarium poae, and Fusarium lateritium.

[0039] The specific pathogens causing wheat root rot include: *Fusarium graminearum*, *Fusarium culmorum*, *Fusarium oxysporum*, *Fusarium proliferatum*, *Fusarium avenaceum*, and *Fusarium moniliforme*. The pathogens causing wheat root rot are complex, including both *Fusarium* species and *Bipolarissorokiniana*. Besides causing root rot, these pathogens also damage other parts of the wheat plant, causing seedling rot, stem base rot, leaf blight in mature plants, and ear rot, with some symptoms overlapping with those caused by pathogens causing wheat stem base rot.

[0040] The beneficial effects of this invention are:

[0041] 1) The pesticide composition of the present invention has high control activity against Fusarium diseases, which is conducive to the effective control of diseases. Under certain mass ratios, the control effect has a synergistic effect.

[0042] 2) The pesticide composition of the present invention can reduce the amount of pesticides used, is safe for crops, and has the effect of increasing yield and ensuring harvest. Detailed Implementation

[0043] To make the technical solution, objectives and advantages of the present invention clearer, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0044] Indoor toxicity testing:

[0045] Indoor bioassay for wheat scab

[0046] Test basis: NY / T 1156.2-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 2: Plate Test for Inhibition of Mycelial Growth of Pathogenic Fungi";

[0047] NY / T 1156.6-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Part 6: Determination of Combined Effects of Mixtures of Fungicides".

[0048] Tested pathogen: Wheat stem rot;

[0049] Wheat plants infected with stem rot were collected from wheat-growing areas in various cities of Shandong Province. Mold-covered areas at the junction of stem nodes and base of typical infected plants were cut and cultured on selective medium. Colonies exhibiting typical Fusarium colony characteristics were then transferred and preserved. Representative strains were selected for single-spore isolation, and molecular identification confirmed the strains as *Fusarium graminearum* and *Fusarium pseudograminearum*.

[0050] Test reagents: All test reagents were provided by the Group's R&D Center.

[0051] Reagent preparation: Dissolve the test drug in acetone first, then dilute it with 0.1% Tween 80 aqueous solution to prepare single-agent stock solutions, and set 5 series of mass concentrations according to the purpose of mixing and the drug activity.

[0052] Melt the PDA medium in a microwave oven and cool it to about 50°C. Following the principle of starting from low concentration and gradually increasing the concentration, take 1 mL of the prepared test solution and 9 mL of PDA medium and add them to a 9 cm diameter petri dish. Mix well to prepare a plate containing the drug at the corresponding concentration.

[0053] Inoculation: Under aseptic conditions, the activated pathogenic fungi were punched into a fungal cake using a 6mm diameter punch. After the drug-containing culture medium solidified, the fungal cake was placed in the center of the culture medium. Finally, the culture dish was sealed with sealing film and placed in an incubator at 28℃ and 70% relative humidity for cultivation. A blank control without the drug was set up. Each treatment was repeated 3 times.

[0054] Investigation: The growth of pathogenic fungal hyphae was investigated based on the growth of hyphae in blank control culture dishes. The diameter of colonies was measured with calipers in millimeters (mm). The diameter of each colony was measured vertically once using the cross-sectional method, and the average value was taken.

[0055] Calculation method: Based on the survey results, the mycelial growth inhibition rate is calculated according to the following formula, in percentage (%), and the calculation result is rounded to two decimal places.

[0056] D = D1 - D2

[0057] In the formula:

[0058] D – Colony growth diameter;

[0059] D1—colony diameter;

[0060] D2 – Diameter of the mushroom cake.

[0061]

[0062] I – Mycelial growth inhibition rate;

[0063] D0—Correlation diameter of the blank control group;

[0064] D t — Diameter of colonies grown after chemical treatment.

[0065] Experimental statistics: Data were processed using probability value analysis. The DPS statistical analysis system was used to analyze the data and determine the toxicity regression line and EC50. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0066] Sun Yunpei's method: The synergistic effect of drug mixtures is evaluated based on the co-toxicity coefficient (CTC). A CTC ≥ 120 indicates a synergistic effect; a CTC ≤ 80 indicates an antagonistic effect; and a CTC < 120 indicates an additive effect.

[0067] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0068]

[0069] In the formula:

[0070] ATI – Actual Measured Toxicity Index of Mixtures;

[0071] S – EC of standard bactericides 50 The unit is milligrams per liter (mg / L);

[0072] M – EC of the mixture 50 The unit is milligrams per liter (mg / L).

[0073] TTI = TI A *P A +TI B *P B

[0074] In the formula:

[0075] TTI – Theoretical Toxicity Index of Mixtures;

[0076] TIA —A. Toxicity index of drug A;

[0077] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0078] TI B —Toxicity index of drug B;

[0079] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0080]

[0081] In the formula:

[0082] CTC – Cotoxicity Coefficient;

[0083] ATI – Actual Measured Toxicity Index of Mixtures;

[0084] TTI – Theoretical Toxicity Index of Mixtures.

[0085] Experimental results:

[0086] Table 1 shows that the mixtures of Cyclobutrifluram and Bifemetstrobin at a mass ratio of 1:40 to 20:1 exhibited additive or synergistic effects on the control of Fusarium graminearum. Among these, the co-toxicity coefficient was >120 at a mass ratio of 1:25 to 20:1, demonstrating a synergistic effect. The highest co-toxicity coefficient (198.801) was observed at a Cyclobutrifluram to Bifemetstrobin ratio of 1:2. 50 The concentration was 3.862 mg / L, indicating a significant synergistic effect.

[0087] Table 1. Indoor assay results of different formulations against Fusarium graminearum

[0088]

[0089]

[0090] Table 2 shows that the mixture of Cyclobutrifluram and Bifemetstrobin at a mass ratio of 1:30 to 22:1 exhibited a synergistic effect against Fusarium graminearum. Specifically, the co-toxicity coefficient was >130 at a mass ratio of 1:18 to 14:1, demonstrating a synergistic effect. The highest co-toxicity coefficient (236.497) was observed at a Cyclobutrifluram to Bifemetstrobin ratio of 2:1.50 The concentration was 1.756 mg / L, indicating a significant synergistic effect.

[0091] Table 2. Indoor assay results of different formulations against Fusarium oxysporum.

[0092]

[0093] Formulation Examples

[0094] Preparation Example 1: 45% Cyclobutrifluram·bifemetstrobin water-dispersible granules (20:25)

[0095] Formulation: Cyclobutrifluram 20%, Bifemetstrobin 25%, Sodium dodecyl sulfate 3%, Naphthalene sulfonate formaldehyde condensate 8%, Sodium fatty alcohol polyoxyethylene ether sulfate 1%, Sodium polycarboxylate 2%, Sodium lignosulfonate 6.5%, Attapulgite 5%, Kaolin to make up the balance;

[0096] Preparation method: According to the formula ratio, add the active ingredients to the carrier, and add surfactants and other functional additives to it. Mix, and after air jet pulverization, add 10-25% water. Then knead, granulate, dry and sieve to obtain water-dispersible granules; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then sieve to obtain the product.

[0097] Preparation Example 2: 18% Cyclobutrifluram·bifemetstrobin emulsifiable concentrate (8:10)

[0098] Formula: Cyclobutrifluram 8%, Bifemetstrobin 10%, BHT 1.2%, propylene carbonate 10%, DMF 8%, dimethyl sulfoxide 5%, cyclohexanone 4%, calcium dodecylbenzenesulfonate 3%, fatty alcohol polyoxyethylene ether 12%, methyl oleate to make up the balance;

[0099] Preparation method: Add the active ingredients to the cosolvent according to the formulation ratio of the example, and add surfactants and other functional additives thereto. Stir and mix evenly in a stirring mixing tank to obtain emulsifiable oil.

[0100] Preparation Example 3: 7.5% Cyclobutrifluram·bifemetstrobin aqueous emulsion (2.5:5)

[0101] Formula: Cyclobutrifluram 2.5%, Bifemetstrobin 5%, Trimethylbenzene 12%, Cyclohexanone 8%, Calcium Dodecylbenzenesulfonate 1%, EO / PO Block Copolymer 4.5%, Glycerol 5%, Glycerin 1.5%, Silicone Defoamer 0.1%, Xanthan Gum 0.15%, Sodium Benzoate 0.1%, Deionized Water to make up the balance;

[0102] Preparation method: According to the formulation ratio in the example, dissolve the active ingredient in the solvent and add the emulsifier to form a homogeneous oil phase. Mix deionized water, antifreeze, and additives together to form a homogeneous aqueous phase. Under stirring, add the oil phase to the aqueous phase, stir evenly, and then shear to the qualified particle size. Then add defoamer, thickener, and other additives, and shear and stir evenly to form a well-dispersed water-emulsion formulation.

[0103] Preparation Example 4: 30% Cyclobutrifluram·bifemetstrobin suspension (14:16)

[0104] Formulation: Cyclobutrifluram 14%, Bifemetstrobin 16%, polyether 4%, naphthalene sulfonate formaldehyde condensate 3%, sodium dioctyl succinate sulfonate 3%, sodium polycarboxylate 1%, magnesium aluminum silicate 0.5%, xanthan gum 0.2%, ethylene glycol 5%, methylisothiazolinone 0.15%, silicone defoamer 0.5%, deionized water to make up the balance;

[0105] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension.

[0106] Field efficacy trials

[0107] Field efficacy trial of wheat stem base rot

[0108] Experimental basis: Refer to the industry standard "Guidelines for Field Efficacy Tests of Pesticides, Part 15: Control of Wheat Fusarium Head Blight by Fungicides (NY / T 1464.15-2007)";

[0109] Experimental crop: Wheat (Yannong 988);

[0110] Experiment location: Wheat field in Lugouya Village, Rizhao City, Shandong Province;

[0111] Experimental target: Diseased wheat samples were collected from the field before the experiment and identified as Fusarium pseudograss after molecular identification;

[0112] Experimental setup: Each cell was randomly distributed across multiple blocks, with a guard row surrounding each cell. Each treatment was replicated four times, with each cell measuring 20m. 2The cells are randomly arranged.

[0113] Application timing: Apply the pesticide once during the wheat's greening stage, and then apply it again 7 days later, for a total of 2 applications. Use conventional spraying methods to evenly spray the pesticide on both sides of the leaves. The spraying equipment is a backpack electric sprayer, and the water consumption is 60L / mu.

[0114] Test reagents:

[0115] Table 3 Experimental Treatment

[0116] Test treatment dosage (g ai / hm) 2 17.5% Cyclobutrifluram·bifemetstrobin emulsion (2.5:5) 60 2 30% Cyclobutrifluram·bifemetstrobin suspension (14:16) 60 3 25% Cyclobutrifluram suspension 50 4 30% Bifemetstrobin suspension 2005 Water control / surface

[0117] Survey and statistical methods: Two surveys were conducted, one at the wheat jointing stage (35 days after application of pesticides, visual observation) and the other at the wheat heading stage. Five sampling points were fixed along the diagonal of each plot, with 60 plants / 100-200 ears surveyed at each point. The wheat disease incidence and disease rate were recorded in detail.

[0118] Grading method:

[0119] Grade 0: No lesions on leaf sheaths or stems;

[0120] Grade 1: Leaf sheath infection, but not invading the stem;

[0121] Grade 2: Lesions invade less than 1 / 4 of the stem circumference;

[0122] Grade 3: Lesions invade less than 1 / 4 to 1 / 2 of the stem circumference;

[0123] Grade 4: Lesions invade less than 1 / 2 to 3 / 4 of the stem circumference;

[0124] Level 5: Lesions invade less than 3 / 4 of the stem circumference, resulting in white ears or no ears due to disease.

[0125] Methods for calculating drug efficacy

[0126] Calculate the disease index and prevention efficacy using the following formula.

[0127]

[0128] Direct impact on crops: Observe whether the pesticide causes phytotoxicity to crops, and record the type and degree of phytotoxicity.

[0129] Effects on other organisms: Any apparent effects on other pests and diseases should be documented, including beneficial or ineffective effects; the effects of the pesticide on other non-target organisms in the test area should also be documented.

[0130] The test results are shown in the table below:

[0131] Table 4. Results of field efficacy trials of various treatment combinations for controlling wheat stem rot.

[0132]

[0133] Note: The percentage of prevention and control efficacy in the table above is the average of each replication; lowercase letters indicate significant differences at the 5% level.

[0134] During the experiment, wheat growth was observed to be good in all treatment plots, and no herbicide damage was observed in any treatment.

[0135] According to the field efficacy trial shown in Table 4 above, the control efficacy of 7.5% Cyclobutrifluram·bifemetstrobin emulsion (2.5:5) and 30% Cyclobutrifluram·bifemetstrobin suspension (14:16) against wheat stem base rot was 82.44% and 82.52% respectively, both showing good control effects.

[0136] No phytotoxicity or impact on crop growth and development was recorded during the experiment, indicating that the tested pesticide was safe for crops within the recommended dosage range. No effects on non-target organisms were found within the dosage range. No extreme weather events such as heavy rainfall or strong winds were recorded during the experiment.

[0137] Through indoor toxicity testing and field trials, the combination of Cyclobutrifluram and Bifemetstrobin described in this invention exhibits excellent control efficacy against wheat stem rot. The pesticide composition or formulation obtained by this invention demonstrates significant efficacy, exhibiting high efficiency, broad spectrum, low residue, long-lasting effect, and strong systemic properties. Furthermore, no phytotoxicity was observed in the experiments, indicating that the enhanced synergistic effect of the resulting pesticide composition or formulation reduces production and usage costs while ensuring crop safety.

[0138] Although the present invention has been described in detail above with general description and specific embodiments, some modifications or improvements can be made to it based on the present invention, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A pesticide composition, characterized in that: It contains active ingredient A and active ingredient B, wherein active ingredient A is Cyclobutrifluram and active ingredient B is Bifemetstrobin, and the mass ratio of active ingredient A to active ingredient B is 1:25 to 20:

1.

2. The pesticide composition according to claim 1, characterized in that, The mass ratio of active ingredient A to active ingredient B is 1:18 to 14:

1.

3. The pesticide composition according to claim 1, characterized in that, Based on a total weight of 100 wt% of the pesticide composition, the sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 1 to 90 wt%.

4. The pesticide composition according to claim 3, characterized in that, The sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 5-80 wt%.

5. The pesticide composition according to claim 1, characterized in that, In addition to the active ingredient, the pesticide composition contains agriculturally permitted auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, binders, or carriers.

6. The pesticide composition according to claim 5, characterized in that, The formulation of the pesticide composition is selected from microemulsions, emulsifiable concentrates, suspension concentrates, water-in-oil emulsions, water-dispersible granules, and seed treatment suspensions.

7. The use of the pesticide composition according to any one of claims 1-6 in the control of plant diseases, characterized in that, The plant diseases mentioned are wheat stem base rot, corn stem base rot, wheat scab, and wheat root rot.

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