Construction method of characteristic chromatogram of ginkgo leaf standard decoction and content determination method of multi-index components

The characteristic map of Ginkgo Leaf Standard Decoction was constructed through high-performance liquid chromatography, which solved the problem of insufficient detection attributes in the existing technology, achieved rapid and stable detection of the ingredients of Ginkgo Leaf Decoction, and improved the quality control ability of Chinese medicine decoctions.

CN120214185AActive Publication Date: 2025-06-27GUANGDONG YIFANG PHARMA
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Patent Information

Application Number
CN202311814943.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2023-12-27
Publication Date
2025-06-27
Estimated Expiration
2043-12-27

AI Technical Summary

Technical Problem

In the prior art, the quality control method of standard decoction of ginkgo leaves lacks special attributes, and traditional acid hydrolysis methods cannot effectively detect prototype components with good water-solubleness, making it difficult to achieve quality control of Chinese medicine decoctions.

Method used

The characteristic map of Ginkgo leaf standard decoction was constructed by high-performance liquid chromatography, and the effective flavonol glycosides were detected through gradient elution procedures and appropriate chromatographic conditions, and the content determination method of multi-index components was established to ensure the specificity and stability of the detection.

Benefits of technology

The rapid and stable detection of multiple index components in standard Ginkgo leaf decoctions has been achieved, which fully reflects the chemical composition characteristics of the decoctions, provides powerful quality control means, and lays the foundation for the standardization and quality evaluation of traditional Chinese medicine decoctions.

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Abstract

The invention relates to a construction method of a specific chromatogram of a ginkgo leaf standard decoction and a content determination method of multi-index components. The construction method of the characteristic chromatogram of the ginkgo leaf standard decoction comprises the following steps: mixing the ginkgo leaf standard decoction with a first solvent, and extracting to prepare a test solution; performing high performance liquid chromatography detection on the test solution, and constructing a characteristic chromatogram of the ginkgo leaf standard decoction; wherein the conditions of the high performance liquid chromatography detection are as follows: a chromatographic column is a T3 chromatographic column; the mobile phase comprises a mobile phase A and a mobile phase B, the mobile phase A comprises methanol and acetonitrile according to a volume ratio of (3-5): 1, the mobile phase B is a phosphoric acid aqueous solution with a volume percentage of 0.08-0.12%, and a gradient elution procedure is adopted. The specific chromatogram constructed by the method has many peaks, prototype components of flavonol glycoside effective components are detected, the detection is rapid and stable, and the specificity is strong.
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