B cell conserved epitope peptide of intracellular lawsonia intracellularis self-transcription protein a and its application in diagnosis

By displaying the B-cell conserved epitope peptide NN8 on the autotranscribed protein A on the surface of Lawsonia suis, combined with agglutination assay, the problem of insufficient specificity and sensitivity in the detection of Lawsonia suis in existing technologies has been solved, realizing rapid, simple and highly specific antibody detection, which is suitable for the accurate diagnosis of Lawsonia suis infection.

CN120248058BActive Publication Date: 2026-07-24YANGZHOU UNIV
4 Cites 0 Cited by

Patent Information

Application Number
CN202510452561.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-04-11
Publication Date
2026-07-24
Estimated Expiration
2045-04-11

AI Technical Summary

Technical Problem

Existing methods for detecting Lawsonia intracellularis in porcines lack specificity and sensitivity, making it difficult to quickly and accurately diagnose Lawsonia intracellularis infection in clinical settings. Traditional serological methods are cumbersome and prone to nonspecific reactions, and current technologies cannot achieve rapid and accurate detection of Lawsonia intracellularis infection in porcines.

Method used

The B-cell conserved epitope peptide NN8 on the self-transcribed protein A on the surface of Lawsonia intracellularis was displayed on the bacterial surface via a recombinant vector. Combined with an agglutination assay directly mediated by NN8-antibody, specific detection of LI antibody was achieved.

Benefits of technology

It enables rapid, simple, highly specific, and sensitive detection of Lawsonia suis intracellularis antibodies, and can detect specific serum antibodies 7 days after vaccination. It is suitable for assessing the level of vaccine immune protection and detecting LI infection.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The application discloses a B cell conservative epitope peptide on a porcine Lawsonia intracellularis (LI) self-transcription protein A (LatA) and application of the B cell conservative epitope peptide in LI specific antibody diagnosis. The B cell conservative epitope peptide of the application has a sequence of NGNGNPAN and is named as NN8. The B cell conservative epitope peptide can be expressed on the surface of an inert carrier S9H bacterium in a functional display mode. An NN8-antibody direct mediation agglutination test is established based on the surface display expression of the S9H bacterium, the LI antibody is specifically recognized by the NN8, and there is no cross reaction with serum antibodies of other porcine infectious diseases. Animal test results show that the method can detect specific serum antibodies 7 days after a weakly virulent strain infects and vaccinates Balb / c mice. The diagnosis method has strong specificity, good sensitivity, convenient operation, and the result is directly determined, and is expected to become a technical means for visual on-site precise and convenient diagnosis and vaccine evaluation of LI infection.
Need to check novelty before this filing date? Find Prior Art