Primer probe combination and kit for detecting spina date seeds and fructus ziziphi spinosae and application of primer probe combination and kit

The design of specific primer probes through LMTIA technology solved the problem of identification of jujube seeds and jujube seeds, achieved rapid, sensitive and specific detection, and solved the difficulty of identification of adulteration and fraud of Chinese medicinal materials.

CN120350151APending Publication Date: 2025-07-22XUCHANG UNIV +1
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Patent Information

Application Number
CN202510391398.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-03-31
Publication Date
2025-07-22

AI Technical Summary

Technical Problem

It is difficult to accurately identify jujube seeds mixed with their fake jujube seeds. Traditional methods are professionally difficult and the results are inaccurate. The molecular biological identification method is costly and the specific PCR method cannot be accurately identified. The conventional real-time fluorescence quantitative PCR detection time is long.

Method used

Gradient melting temperature isothermal amplification (LMTIA) technology is used to design specific primer probes for jujube seeds and jujube seeds, establish a rapid detection method, and use the combination of LMTIA primer probes for detection.

Benefits of technology

The rapid, sensitive and specific detection of jujube seeds and jujube seeds are achieved, with the detection limit reaching 1pg/μL, which can identify the adulteration and fraud of Chinese medicinal materials, and improve the identification efficiency and accuracy.

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Abstract

The invention discloses a primer probe combination and a kit for detecting spina date seeds and fructus ziziphi spinosae and application of the primer probe combination and the kit, and belongs to the technical field of molecular detection. The LMTIA primer and probe combination provided by the invention comprises SZaoR-F1, SZaoR-B1, SZaoR-LF, SZaoR-LB, LZaoR-F3, LZaoR-B3, LZaoR-LB, SZR-LF Pr and LZR-LB Pr, and is characterized in that the SZaoR-LF and the LZaoR-LB are used as primers and probes of the LZaoIA; according to the present invention, the LMTIA technology is adopted, the LMTIA specific primers and the probe for the spina date seed and the jujube kernel are designed, the rapid detection method for the components of the spina date seed and the jujube kernel is established, and the technical support is provided for the identification of the traditional Chinese medicinal material spina date seed and the jujube kernel and the adulteration and counterfeiting identification of the traditional Chinese medicinal material.
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